Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,323 T→A 5.3% intergenic (‑59/‑201) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3230TA 5.3% 396.3 / 7.1 138intergenic (‑59/‑201)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base T (59/71);  new base A (4/4);  total (63/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

Only 100 of 142 total aligned reads displayed.

CCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTC  >  NC_000913/2839174‑2839471
                                                                                                                                                     |                                                                                                                                                    
ccTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCTTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCt                                                                                                                                                      <  1:1438412/150‑1 (MQ=255)
ccTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCt                                                                                                                                                      >  1:959861/1‑150 (MQ=255)
 cTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCtt                                                                                                                                                     <  2:658305/150‑1 (MQ=255)
    gCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGca                                                                                                                                                  <  1:670275/150‑1 (MQ=255)
     cTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCCCCGTTTGCCGATTGTCCTTGcac                                                                                                                                                 >  2:175737/1‑150 (MQ=255)
     cTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGcac                                                                                                                                                 >  2:1858843/1‑150 (MQ=255)
       gACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACaa                                                                                                                                               <  1:510671/150‑1 (MQ=255)
            aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                          <  2:552687/150‑1 (MQ=255)
            aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                          <  1:1476668/150‑1 (MQ=255)
            aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                          <  1:1536591/150‑1 (MQ=255)
            aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGACACTACCAGCATCGTCGTCATCATTTTCCTCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTGTGCCGATTGTGCAAGGACAATcggc                                                                                                                                          <  2:265790/150‑1 (MQ=255)
             gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                         <  1:892175/150‑1 (MQ=255)
              ccATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgg                                                                                                                                        >  2:95412/1‑150 (MQ=255)
              ccATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgg                                                                                                                                        <  1:1743459/150‑1 (MQ=255)
                      tGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatt                                                                                                                                >  1:1745118/1‑149 (MQ=255)
                       gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGCTTGTCCTTGCACAATCGGCGGGAAAAatat                                                                                                                               >  2:1858823/1‑150 (MQ=255)
                       gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCAGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatat                                                                                                                               <  2:1519468/150‑1 (MQ=255)
                               gcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTACAGCATCGTCGTCATCATTTTAATCCCATTAAACCCAATCAACGCACTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGtta                                                                                                                       >  2:1884865/1‑148 (MQ=255)
                                 gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                                                                                                     <  2:66172/150‑1 (MQ=255)
                                 gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                                                                                                     <  2:1377873/150‑1 (MQ=255)
                                 gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATAAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                                                                                                     >  1:661750/1‑150 (MQ=255)
                                         gTCGCTTTTTAAACACCGGCGCGCTTCTACACTTACAACATAGTCGTCATCATTATCATCCCTTTACACGCAATCAAACCACTGTACTGCAACCTTTGCCGATAGTCCATGCACAATCGGCAGTAAAAAAATTCACCTGAACGGTTAcaa                                                                                                             >  2:558725/1‑149 (MQ=255)
                                           cGCATTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGAAATCAACGCAGTGTACTGCACCGTTTTCCGATTGTCCTTGCACACTCGGCGGGAAAAATATTCATGTGACCGGTTTCACaa                                                                                                           >  2:744654/1‑150 (MQ=255)
                                            gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCCCCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAACTATTCAGGTGACCGGTTTCACaaa                                                                                                          >  1:225389/1‑150 (MQ=255)
                                            gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTGTCACaaa                                                                                                          >  1:1517119/1‑150 (MQ=255)
                                              ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCCACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAata                                                                                                        <  2:1132827/150‑1 (MQ=255)
                                               tttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTAACAAatat                                                                                                       >  2:1185221/1‑150 (MQ=255)
                                                ttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAAtata                                                                                                      <  1:546052/150‑1 (MQ=255)
                                                      ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt                                                                                                <  1:1578052/150‑1 (MQ=255)
                                                       cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                               <  2:1653570/150‑1 (MQ=255)
                                                        ccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGa                                                                                              >  1:781187/1‑150 (MQ=255)
                                                            cgctcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTTCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACaa                                                                                          <  1:352801/150‑1 (MQ=255)
                                                            cgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACaa                                                                                          >  1:96619/1‑150 (MQ=255)
                                                               gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc                                                                                       <  1:1325390/150‑1 (MQ=255)
                                                                cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa                                                                                      >  1:206421/1‑150 (MQ=255)
                                                                 ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAATCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc                                                                                     <  2:1006436/150‑1 (MQ=255)
                                                                  tCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAct                                                                                    <  2:739970/150‑1 (MQ=255)
                                                                  tCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTATACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCCGCAAAAATATTCAGGTGACCGGTTCCACAAATATAAAAAATGAACTATTCaat                                                                                    >  1:1166183/1‑148 (MQ=255)
                                                                    gCCACTTCCAGCATCGTCGTCATCATTTTCTTCCCTTTACACGCCATCAACGCAGTGTACAGCACCGTTTGCCGATTGTACTTGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                  >  2:368148/1‑150 (MQ=255)
                                                                    gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                  >  2:1576641/1‑150 (MQ=255)
                                                                     ccACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTAActcta                                                                                 >  1:1791473/1‑149 (MQ=255)
                                                                      cACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctct                                                                                <  1:1668482/150‑1 (MQ=255)
                                                                         ttCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATGAACGCAGTGTACTGGACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATTAACAATTCACTCTCTTGc                                                                             <  1:837317/150‑1 (MQ=255)
                                                                           ccAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCtt                                                                           >  2:1087483/1‑150 (MQ=255)
                                                                            catcATCGTCGGCATGATTTTAATCCCTTTACACGCAATCAACCCTTTGTACTTCTCCGTTTTGGGATTGGCGTTGCTCATTCGGCGGGAAAAATATTCAGTGGACCGGTTTCACTAATATTAAAAATCAACATTTCACTCTCTTGCttc                                                                          <  2:630768/147‑2 (MQ=255)
                                                                            cAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTa                                                                          <  2:959861/150‑1 (MQ=255)
                                                                            cAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTa                                                                          <  1:465357/150‑1 (MQ=255)
                                                                              gCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAtt                                                                        <  1:426467/150‑1 (MQ=255)
                                                                               cATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTAttt                                                                       <  1:1344568/150‑1 (MQ=255)
                                                                                   gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                   >  1:1839540/1‑150 (MQ=255)
                                                                                      gtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAATATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACa                                                                <  2:439985/150‑1 (MQ=255)
                                                                                      gtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACa                                                                <  2:843596/150‑1 (MQ=255)
                                                                                      gtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTAACAAATATAAAAAATGCACAATTCACTCTCTTGCTTATTTAGTGACa                                                                <  1:755302/150‑1 (MQ=255)
                                                                                        catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                              <  1:1851214/150‑1 (MQ=255)
                                                                                        catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                              >  2:216977/1‑150 (MQ=255)
                                                                                            atTTTCATCCCTTTACACGCAATTAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                          >  1:721126/1‑150 (MQ=255)
                                                                                            atTTTCATCCCTTTACACGCAATCAACGCAGTGTACAGCACAGTTTGCCGATTGTCCTTGCACAAACGGCGGGAAAAATATACAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACACTCTTGCTTATTTAGTGACAACTAtt                                                          >  1:1673282/1‑150 (MQ=255)
                                                                                                    cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAttt                                                  <  2:575191/150‑1 (MQ=255)
                                                                                                       tttACACTAAATCATCTCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTTACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgt                                               <  1:1113871/150‑1 (MQ=255)
                                                                                                       tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgt                                               <  1:593835/150‑1 (MQ=255)
                                                                                                        ttccacGCAATCAACGCAGTGTACTTCAGCGGTTGCCGTTTGTCCATGCACAAGCGGCGGGAAAAATAATCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                              <  2:365157/147‑1 (MQ=255)
                                                                                                        ttACACGCAATCAACGCAGTGTTCAGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGTAAAATAATCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                              <  2:1481810/150‑1 (MQ=255)
                                                                                                        ttACAAGCAATCAACGCAGTGTACTGCACCGTTTGCAGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTGCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                              <  2:1163188/150‑1 (MQ=255)
                                                                                                           cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACATCTATTCATGATTTTGTGaaa                                           >  1:1446743/1‑150 (MQ=255)
                                                                                                           cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                           >  2:615292/1‑150 (MQ=255)
                                                                                                           cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                           >  2:1663774/1‑150 (MQ=255)
                                                                                                             cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAATcc                                         >  2:1776213/1‑150 (MQ=255)
                                                                                                               cAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgt                                       >  1:1552844/1‑149 (MQ=255)
                                                                                                                 aTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACTATTCACTCTCTTGCTTATTTCGTGACAACTATTCATGATTTTGTGAAACCGGtt                                     <  2:1343999/150‑1 (MQ=255)
                                                                                                                   cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                   <  1:1799854/150‑1 (MQ=255)
                                                                                                                   cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                   <  1:1705535/150‑1 (MQ=255)
                                                                                                                    aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                  >  2:921114/1‑150 (MQ=255)
                                                                                                                    aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                  >  2:1241112/1‑150 (MQ=255)
                                                                                                                     ccGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCtt                                 <  2:844760/149‑1 (MQ=255)
                                                                                                                      cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTa                                <  2:1072600/150‑1 (MQ=255)
                                                                                                                            gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg                          >  2:143242/1‑150 (MQ=255)
                                                                                                                            gtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAAAATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg                          <  1:117774/150‑1 (MQ=255)
                                                                                                                             tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGt                         >  2:415810/1‑150 (MQ=255)
                                                                                                                              aCTTCACCGTTTGCCGATTGTCCTTGCACAATCGGCGTGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCATATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGtt                        <  1:266949/150‑1 (MQ=255)
                                                                                                                                tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATCTTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTc                      <  2:493327/150‑1 (MQ=255)
                                                                                                                                tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTc                      >  2:456279/1‑150 (MQ=255)
                                                                                                                                 gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                     >  2:1597329/1‑150 (MQ=255)
                                                                                                                                  cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAg                    <  2:842017/150‑1 (MQ=255)
                                                                                                                                    ccgattgccgattgTCCTTGCACAATAGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                  <  1:340924/150‑1 (MQ=255)
                                                                                                                                    ccGTTTGCCTATTGTCATTTCAAAATCGGCGGGAAAAATATTCAGGTGACCTGTTTCACAAATATAAATAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                  <  1:225336/150‑1 (MQ=255)
                                                                                                                                    ccGTTTGCCGATTGTCCTTTCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                  <  1:61070/150‑1 (MQ=255)
                                                                                                                                      gTTTGCCGATTGTCCTTTCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc                <  1:907973/150‑1 (MQ=255)
                                                                                                                                       tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCg               >  2:2008/1‑150 (MQ=255)
                                                                                                                                         tGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc             >  2:785362/1‑150 (MQ=255)
                                                                                                                                          gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa            >  2:1488543/1‑150 (MQ=255)
                                                                                                                                            cGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtt          >  2:193472/1‑150 (MQ=255)
                                                                                                                                                tGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGTTTTCTTAATTCCGTTTCAGCATCGGCATTTTTc      >  1:193922/1‑150 (MQ=255)
                                                                                                                                                tGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTc      <  2:661750/150‑1 (MQ=255)
                                                                                                                                                tGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTc      <  1:175737/150‑1 (MQ=255)
                                                                                                                                                 gTCCTTGCACAATCGGCGGGAAAAATATTCAGGTTACCGGTTCCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTAAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc     <  2:1166183/150‑1 (MQ=255)
                                                                                                                                                 gTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc     >  2:440616/1‑150 (MQ=255)
                                                                                                                                                  tCCTTGCACAATCGGCGGGAAAAATATTCCTGGGACCGGTTTCACAAATAAAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGTTTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg    <  2:1227573/150‑1 (MQ=255)
                                                                                                                                                   ccTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTTTGAATCCGGTTTCTTAATTACTTTTCAGCATCGGCATTTTTCCGt   >  2:935381/1‑150 (MQ=255)
                                                                                                                                                   ccTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGt   >  1:401418/1‑150 (MQ=255)
                                                                                                                                                    cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCAAAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTTACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTc  <  2:79208/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTC  >  NC_000913/2839174‑2839471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: