Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,859,832 G→C 5.1% G25R (GGT→CGT)  pphB → serine/threonine‑specific protein phosphatase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,859,8320GC 5.1% 335.3 / 7.9 113G25R (GGT→CGT) pphBserine/threonine‑specific protein phosphatase 2
Reads supporting (aligned to +/- strand):  ref base G (48/59);  new base C (4/2);  total (52/61)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 119 total aligned reads displayed.

GGGAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTCGGTT  >  NC_000913/2859683‑2859981
                                                                                                                                                     |                                                                                                                                                     
gggAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAAAGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATg                                                                                                                                                       >  1:1640364/1‑150 (MQ=255)
gggAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAAACAATCCCCAACAAAATCGCCATATATGGTTCGTTGGTGATATTCATg                                                                                                                                                       >  1:862522/1‑150 (MQ=255)
 ggAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATgg                                                                                                                                                      >  1:722664/1‑150 (MQ=255)
  gAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATATGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGt                                                                                                                                                     <  2:720359/150‑1 (MQ=255)
  gAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGt                                                                                                                                                     <  1:868498/150‑1 (MQ=255)
      atTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAat                                                                                                                                                 >  2:1649644/1‑150 (MQ=255)
      atTAACGACAACTGACGAATAAAATAAAAACACACTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACTCTATCAAAAAATCAATGCCCATCACTATCTCCATAAATAGGTCGTTGGTGATTTTCATCGTGAat                                                                                                                                                 >  1:156639/1‑150 (MQ=255)
       ttAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAata                                                                                                                                                <  1:341028/150‑1 (MQ=255)
        tAAAGACAAATGACTAATAAACTAAAAACAACCTGTATAATATGAAAGCTTATTATACATGGTAAAACCATGCCATCTACACGCTATCAAAAAATCACTGCACATCACTATCGCCAAATATGGGTCGTTGGTGATATTCATGGTGAatat                                                                                                                                               <  1:299573/150‑1 (MQ=255)
         aaCGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATc                                                                                                                                              >  2:1302625/1‑150 (MQ=255)
         aaCGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATc                                                                                                                                              <  2:1121723/150‑1 (MQ=255)
          aCGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCa                                                                                                                                             >  2:1033253/1‑150 (MQ=255)
           cGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCACCATGAATATCac                                                                                                                                            <  2:1117380/150‑2 (MQ=255)
            gACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCACCATGAATATCacc                                                                                                                                           <  1:150688/150‑3 (MQ=255)
              cAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGtta                                                                                                                                         <  1:793422/150‑1 (MQ=255)
                aCTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACAATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGttttt                                                                                                                                       >  2:203350/1‑150 (MQ=255)
                   gACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGTTGATATTCATGGTGAATATCAGTTATTACa                                                                                                                                    >  2:248069/1‑150 (MQ=255)
                      taataaaataaAAACACCCTTTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGGCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATc                                                                                                                                 <  2:301693/149‑1 (MQ=255)
                        ataaaataaaAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATccc                                                                                                                               <  2:109981/150‑1 (MQ=255)
                        aaaaaataaaaACACCCTCTATAATAGTAAAGATTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATccc                                                                                                                               <  2:1191243/148‑1 (MQ=255)
                         taaaataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCg                                                                                                                              >  1:585012/1‑150 (MQ=255)
                         taaaataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCg                                                                                                                              >  2:1172032/1‑150 (MQ=255)
                         taaaataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCg                                                                                                                              <  2:1825509/150‑1 (MQ=255)
                          aaaataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGc                                                                                                                             <  1:1884814/150‑1 (MQ=255)
                          aaaataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGc                                                                                                                             <  1:1780703/150‑1 (MQ=255)
                           aaataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCTCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCt                                                                                                                            >  1:1135681/1‑150 (MQ=255)
                            aataaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCtt                                                                                                                           <  2:148062/150‑1 (MQ=255)
                              taaaaACACCCTGTATAATAGGACAGCTTATTTTACAGGGTAAAACCATGCCATCTTCACGCTATCAAAACATCAATGCCCATCACTATCGCCATCTATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTAc                                                                                                                         <  1:142552/150‑1 (MQ=255)
                              taaaaACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTTTCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCTTGGTGAATATCAGTTATTACAATCCCGCTTAc                                                                                                                         >  2:327147/1‑150 (MQ=255)
                               aaaaaCACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACa                                                                                                                        <  2:394826/150‑1 (MQ=255)
                                aaaaCACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACAt                                                                                                                       >  2:1583941/1‑150 (MQ=255)
                                         tGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAActctct                                                                                                              <  2:1639205/150‑1 (MQ=255)
                                             taataGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCttttt                                                                                                          <  1:1576635/150‑1 (MQ=255)
                                                 tggAAAGCTTATTTTACAGGGTAAAACCATGGCATCTACACGCTATCAAAAAATCAATGCCCATCAATATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTccc                                                                                                      <  2:649009/149‑1 (MQ=255)
                                                 aGGCAAGCTTATTTTACAGGGTAAAGCCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATTTGGGTCGTTGGTGATATTCATGCTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCttttttaac                                                                                                      >  2:1202674/1‑147 (MQ=255)
                                                  ggAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTcccc                                                                                                     >  1:1555119/1‑150 (MQ=255)
                                                   gAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCa                                                                                                    >  1:403410/1‑150 (MQ=255)
                                                         ttATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCAATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCg                                                                                              >  2:1608483/1‑150 (MQ=255)
                                                            ttttACAGTGTAAAAGCATGCCATCTACACGCTATCAAAAAATCAATGCCGATCACTATCGCCATATATGGGTCGTTTGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGact                                                                                           <  1:1169719/150‑1 (MQ=255)
                                                            ttttACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGact                                                                                           <  1:624735/150‑1 (MQ=255)
                                                                aCAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGacttact                                                                                       >  1:386967/1‑150 (MQ=255)
                                                                 cAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGacttactt                                                                                      >  2:738831/1‑150 (MQ=255)
                                                                 cAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGacttactt                                                                                      >  1:567055/1‑150 (MQ=255)
                                                                    ggTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTAtt                                                                                   >  1:1511296/1‑150 (MQ=255)
                                                                     gTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTAttt                                                                                  >  2:1020847/1‑150 (MQ=255)
                                                                     gTAAAACCATGCCATCAACACGCTATCAAATATCATTGCACATCAGTATCGCCATATATTTGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACTATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTc                                                                                 <  2:722565/150‑1 (MQ=255)
                                                                         aaCCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGt                                                                              <  1:1302625/150‑1 (MQ=255)
                                                                             aTGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGc                                                                          <  1:149251/150‑1 (MQ=255)
                                                                             aTGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGc                                                                          <  2:132895/150‑1 (MQ=255)
                                                                               gCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGa                                                                        <  2:1254874/150‑1 (MQ=255)
                                                                                ccATCTACACGCTATCAAAAAATCAATGCCCATCACTCTCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGat                                                                       <  1:1036346/150‑1 (MQ=255)
                                                                                   tCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGataat                                                                    >  2:1019588/1‑150 (MQ=255)
                                                                                   tCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATAGACTTACTTATTTCTGTCGGCGataat                                                                    >  1:935946/1‑150 (MQ=255)
                                                                                      acacGCCATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATAtt                                                                 >  1:790718/1‑150 (MQ=255)
                                                                                         cccTATAAAAAAATCAATGCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATc                                                             <  2:141878/148‑1 (MQ=255)
                                                                                         cGCTATCAAAAAATCAATTACCATCACTAACGCCATATATGGGTCGTTGGTGATAATCATGGTCAAAAACAGATATTACAATCACCCTTACATCAACTCTCTTATTTCCCCAAAATCGACTTACTTATTTCTGTAGACGATAATATTGAt                                                              >  1:1165910/1‑150 (MQ=255)
                                                                                         cGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGAt                                                              >  1:898043/1‑150 (MQ=255)
                                                                                          gCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTTAta                                                             >  1:57481/1‑149 (MQ=255)
                                                                                          gCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATc                                                             >  2:428584/1‑150 (MQ=255)
                                                                                          gCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATc                                                             >  2:597133/1‑150 (MQ=255)
                                                                                              tCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTgg                                                         >  1:1127202/1‑150 (MQ=255)
                                                                                              tCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTgg                                                         <  1:881929/150‑1 (MQ=255)
                                                                                              tCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTgg                                                         <  1:126913/150‑1 (MQ=255)
                                                                                              tCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTACCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATAGTgg                                                         >  1:1773671/1‑150 (MQ=255)
                                                                                               cAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTAAATGAACTCTCTTTTTTCCGAAAAATCGACTTACTTATTTCTTTCTTCGATAATATTGATCGTGGa                                                        >  1:306884/1‑150 (MQ=255)
                                                                                               aaaaataTCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGa                                                        <  1:1031385/149‑1 (MQ=255)
                                                                                               aaaaaaaTCAATGCCCATCACTATCTCCATATATGTGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGa                                                        <  2:1182343/149‑1 (MQ=255)
                                                                                                 aaaaaTCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATAACAATAACGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTTTTTCTGTCGGCGATAATATTGATCGTGGAcc                                                      >  1:729203/1‑150 (MQ=255)
                                                                                                  aaaaTCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCg                                                     <  1:1033253/150‑1 (MQ=255)
                                                                                                    aaTCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGga                                                   <  1:1868695/150‑1 (MQ=255)
                                                                                                          tGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCt                                             <  2:57951/150‑1 (MQ=255)
                                                                                                            cccATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTg                                           <  1:1004617/150‑1 (MQ=255)
                                                                                                            cccATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTg                                           <  1:1172032/150‑1 (MQ=255)
                                                                                                            cccATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCACAAAAAACGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTg                                           >  2:1911407/1‑150 (MQ=255)
                                                                                                              cATCACTATCGCCATATATGGGGCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGAc                                         <  2:161472/150‑1 (MQ=255)
                                                                                                               aTCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTAATACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTACTGTCGGCGATAATATTGATCGTTGACCGGAGAGTCTTGAct                                        >  1:1411426/1‑149 (MQ=255)
                                                                                                                       cGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTgcgc                                >  1:1850702/1‑150 (MQ=255)
                                                                                                                        gCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTAATTATATCTGTCGGCGAAAATATTGATCGAGGACCGGAGAGTCTTGACGTCCTGCGcc                               >  2:718202/1‑150 (MQ=255)
                                                                                                                          cATATATGGGTCGTTGGTGATATTCATGGTGTATATCAGTTTTTACAATCCCGCTTACTTCAACTCTCTTTTTTGCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTg                             <  2:1764208/150‑1 (MQ=255)
                                                                                                                           atatatGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGc                            <  1:1322473/150‑1 (MQ=255)
                                                                                                                           atatatGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGc                            <  1:1658409/150‑1 (MQ=255)
                                                                                                                            tatatGGGTCGTTGGTGATATTCATGTTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCt                           <  2:861486/150‑1 (MQ=255)
                                                                                                                               atGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGCCTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTaaa                        >  2:1426487/1‑150 (MQ=255)
                                                                                                                                tGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAaac                       <  1:1614438/150‑1 (MQ=255)
                                                                                                                                tGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAaac                       <  1:690269/150‑1 (MQ=255)
                                                                                                                                tGGGGCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAaac                       <  2:348777/150‑1 (MQ=255)
                                                                                                                                    tCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAaaccaac                   <  1:288699/150‑1 (MQ=255)
                                                                                                                                      ataactGATATTCACCATGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTACTGCGCCTGCTAAACCAAccc                 >  1:674868/6‑150 (MQ=255)
                                                                                                                                         actGATATTCACCATGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTgg              >  2:1480557/3‑150 (MQ=255)
                                                                                                                                         actGATATTCACCATGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGAGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTgg              >  1:1068107/3‑150 (MQ=255)
                                                                                                                                          gTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGt             >  2:1744764/1‑150 (MQ=255)
                                                                                                                                            gATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTACCCAAAATCGACTTAATTATTTCTGTCGGCGTTAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGttt           <  1:1012755/150‑1 (MQ=255)
                                                                                                                                             atatTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTa          <  2:1682023/150‑1 (MQ=255)
                                                                                                                                              tatTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTAc         >  2:391701/1‑150 (MQ=255)
                                                                                                                                                 tCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTc      <  1:874031/150‑1 (MQ=255)
                                                                                                                                                  cATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTCg     <  2:1119157/150‑1 (MQ=255)
                                                                                                                                                  cATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTCg     <  1:1583941/150‑1 (MQ=255)
                                                                                                                                                  cATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTCg     >  1:1487230/1‑150 (MQ=255)
                                                                                                                                                    tGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTCGGt   >  2:44645/1‑150 (MQ=255)
                                                                                                                                                     ggTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTGATTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTCATCGTGGACCGGAGAGTCTTGACTTCCTGCGCCAGCTAAACCAACCCTGGATAACGTCGGtt  >  2:1310762/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGCTTATTTTACAGGGTAAAACCATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATATCAGTTATTACAATCCCGCTTACATCAACTCTCTTTTTTCCCCAAAATCGACTTACTTATTTCTGTCGGCGATAATATTGATCGTGGACCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAACCCTGGTTTACGTCGGTT  >  NC_000913/2859683‑2859981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: