Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,322 C→T 5.7% intergenic (‑49/‑270) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3220CT 5.7% 415.8 / 9.4 139intergenic (‑49/‑270)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (68/63);  new base T (4/4);  total (72/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 149 total aligned reads displayed.

AATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACAC  >  NC_000913/2900173‑2900471
                                                                                                                                                        |                                                                                                                                                     
 aTGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTAAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTccccc                                                                                                                                                      <  2:457255/150‑1 (MQ=255)
         gcTAAACAGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGTATAAAAAATCCATTGATAACGCATTGAGACATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCA‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                                                                                                               <  2:417692/149‑1 (MQ=255)
         ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                              >  2:236366/1‑150 (MQ=255)
         ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                              >  1:203477/1‑150 (MQ=255)
           tAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGa                                                                                                                                            <  2:109676/150‑1 (MQ=255)
            aaaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                                                                                                           >  2:936641/1‑150 (MQ=255)
             aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCGGGGAGAAAAAATCCATTGCGAACGCATTGAGAGATTAGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGAGGTAGGGAAg                                                                                                                                          >  2:89758/1‑150 (MQ=255)
             aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAg                                                                                                                                          <  2:1034290/150‑1 (MQ=255)
             aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAg                                                                                                                                          >  1:1778286/1‑150 (MQ=255)
                 gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAc                                                                                                                                      >  1:814173/1‑150 (MQ=255)
                   tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCt                                                                                                                                    >  1:1888959/1‑150 (MQ=255)
                   tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATGGCGAGATTCGATTGACATAGTTAGCTCCAATTTCAGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                    <  2:1346992/150‑1 (MQ=39)
                    aTTCCCCACGGTAACTATTTCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAGCTTCCCTCTCGCGGGGGAAGTTACCtt                                                                                                                                   <  1:165384/150‑1 (MQ=255)
                    aTTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                                                                                                   <  1:1217101/150‑1 (MQ=255)
                      tCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                                                                                                 <  2:552262/150‑1 (MQ=255)
                      tCCCAATGGTTACTATTTCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTTACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑TAAAATAATGAAAGGTAAGTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                                                                                                 <  1:1236386/150‑1 (MQ=255)
                       cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtct                                                                                                                                >  1:1729270/1‑150 (MQ=255)
                       cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAACAAATGTAACTTCCCCCGCGAGAGGGAAGTTACCtttaa                                                                                                                                >  2:1439954/1‑148 (MQ=255)
                        ccACCGGTAACAATTGCGTTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTTACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                               <  1:1768207/150‑1 (MQ=255)
                        ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                               <  2:1109022/150‑1 (MQ=255)
                        ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGAGAGAGGGAAGTTACCTTtatc                                                                                                                               >  2:1276601/1‑150 (MQ=255)
                         cACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCa                                                                                                                              <  2:620307/150‑1 (MQ=255)
                           ccGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCAtt                                                                                                                            >  1:1605649/1‑150 (MQ=255)
                            cGGTAACAATTGCGGTTTTACCTTTCAGGTAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTa                                                                                                                           <  2:1160185/150‑1 (MQ=255)
                               tAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtat                                                                                                                        >  1:1311847/1‑148 (MQ=255)
                                   aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                    <  2:609428/150‑1 (MQ=255)
                                     ttGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                                                                  >  2:668374/1‑150 (MQ=255)
                                          gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                             <  2:1565279/150‑1 (MQ=255)
                                          gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                             <  2:1491681/150‑1 (MQ=255)
                                          gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                             >  1:510433/1‑150 (MQ=255)
                                                ccTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCAGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATaa                                                                                                       >  2:1191138/1‑150 (MQ=255)
                                                 cTTTCAGGGATAAAAAATCCATTGAGAAAGCATTGATAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                                                                                                         <  2:640428/150‑1 (MQ=255)
                                                 cTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAt                                                                                                      >  1:1110879/1‑150 (MQ=255)
                                                  tttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtt                                                                                                     >  1:132572/1‑150 (MQ=255)
                                                    tCAGGGAGAAAAAAACCATTGAGAACGCAATCAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATAAtgag                                                                                                   <  1:921424/150‑4 (MQ=255)
                                                     cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttttt                                                                                                  <  2:667993/150‑1 (MQ=255)
                                                       gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                <  1:1000323/150‑1 (MQ=255)
                                                       gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGAAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                <  1:671146/150‑1 (MQ=255)
                                                        ggATAAAAAATCCATTGAGAACGCATTGATAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAATGC‑‑‑AAAAAAAAATAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                                                               <  1:1070181/150‑1 (MQ=255)
                                                          agaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt                                                                                             >  2:187729/1‑150 (MQ=255)
                                                              aaaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg                                                                                         >  2:1652987/1‑150 (MQ=255)
                                                                    cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                   >  1:23889/1‑150 (MQ=255)
                                                                      ttGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACt                                                                                 <  1:936641/150‑1 (MQ=255)
                                                                         agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCt                                                                              <  2:966252/150‑1 (MQ=255)
                                                                           aaCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTaa                                                                            >  2:784902/1‑150 (MQ=255)
                                                                            aCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAt                                                                           <  1:558985/150‑1 (MQ=255)
                                                                               cATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                                                                        >  1:799231/1‑150 (MQ=255)
                                                                               cATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                                                                        <  1:1610254/150‑1 (MQ=255)
                                                                                 ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCAGTAAGTA‑‑‑AAAAAAAAAGAAATGTAACTTCCCCCGCGAGAGTGAAGTTACCTTTCTCATTATCTCTGGTAACTGGATATAATTTTTAGCATAAGGGCATAACTGCTAAtgtgtt                                                                      <  1:674962/150‑3 (MQ=255)
                                                                                  tGAGATATTCGATAGACATAGTTAGCCCCAATTTCCGTAAGGC‑‑‑AAAAAAAACGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTAATTATCTCTGGTTACTGTAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                     <  2:47579/150‑1 (MQ=255)
                                                                                   gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                                                                    <  2:1401897/150‑1 (MQ=255)
                                                                                     gagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCCTTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTa                                                                  >  2:1201976/1‑150 (MQ=255)
                                                                                     gagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGAATAAGGGCATAACTGCTAATGTGATCGTa                                                                  >  1:241020/1‑150 (MQ=255)
                                                                                       gaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAACAGAAAGGTAACTTCCACCGATAGAGTGAAGTTCCCTTTCTCATTAACTCTTGTAACTGGAGATAATTTTTCGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                >  1:106872/1‑150 (MQ=255)
                                                                                       gaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                >  1:1359955/1‑150 (MQ=255)
                                                                                          tCGATTGACATAGTTATCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcac                                                             <  2:123844/150‑1 (MQ=255)
                                                                                            gATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcacat                                                           >  1:32333/1‑149 (MQ=255)
                                                                                            gATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAg                                                           >  2:1034435/1‑150 (MQ=255)
                                                                                              ttGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATTATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg                                                         >  2:366002/1‑150 (MQ=255)
                                                                                               tGACATAGTTAGCTCCAATTTCCGAAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt                                                        <  1:1152408/150‑1 (MQ=255)
                                                                                                  cATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTATCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                     >  1:511793/1‑150 (MQ=255)
                                                                                                  aaTAGTTAGCTCCAATTTCCGTAATGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                     <  1:437923/149‑1 (MQ=255)
                                                                                                     aGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATAtt                                                  >  2:874516/1‑150 (MQ=255)
                                                                                                          gCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                             <  2:1631891/150‑1 (MQ=255)
                                                                                                           cTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                            >  1:1542696/1‑150 (MQ=255)
                                                                                                            tCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTg                                           >  1:793887/1‑150 (MQ=255)
                                                                                                             ccAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGt                                          >  2:1227499/1‑150 (MQ=255)
                                                                                                                   tCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                    >  1:750798/1‑150 (MQ=255)
                                                                                                                     cGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGAAATCACAGTGTGATATTCGTATTGTCAaataat                                  >  2:122770/1‑150 (MQ=255)
                                                                                                                      gTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                 >  1:609702/1‑150 (MQ=255)
                                                                                                                      gTAAGGA‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                 <  1:1322708/150‑1 (MQ=255)
                                                                                                                         aGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTa                              <  2:1461671/150‑1 (MQ=255)
                                                                                                                          ggC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaa                             >  1:739644/1‑150 (MQ=255)
                                                                                                                          ggC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaa                             >  2:242505/1‑150 (MQ=255)
                                                                                                                           gC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                            >  2:610446/1‑150 (MQ=255)
                                                                                                                            c‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAGCTTTCTCATAATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                           >  2:293751/1‑150 (MQ=255)
                                                                                                                            c‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                           <  1:146492/150‑1 (MQ=255)
                                                                                                                            c‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                           >  2:1864369/1‑150 (MQ=255)
                                                                                                                                 aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                         <  2:1814800/150‑1 (MQ=255)
                                                                                                                                 aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                         <  2:1763627/150‑1 (MQ=255)
                                                                                                                                 aaaaaaaaGAAACGTAACATCCCCCGCGACATGGAAGTTACCTTTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATATGGGCATAACTGCTAATGTGATCGTAATCACAGGGTGATATTCGTATTGTCAAATAATTTTAAAAAg                         <  2:312734/150‑1 (MQ=255)
                                                                                                                                   aaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACGTTTCGCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGATAATGTGATCGTAATCACAGTGTGATATTGGTATTGTCAAATAATTTTAAAAAGCa                       >  1:215455/1‑150 (MQ=255)
                                                                                                                                    aaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAGCTTTCTCATTATCTCTTGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAg                      <  2:1412410/150‑1 (MQ=255)
                                                                                                                                    aaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAg                      <  2:1600147/150‑1 (MQ=255)
                                                                                                                                       aagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAg                   >  1:128002/1‑150 (MQ=255)
                                                                                                                                           aagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg               <  2:1785412/150‑1 (MQ=255)
                                                                                                                                           aacccaaCTTCCCCCGCGACAGCGAAGTTACCATACACATTATCTCTGGTCACCGCCGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGGGTGATATTCGAATTGTCAAATAATTTTAAAAAGCAGAAGCGTg               <  2:1248209/145‑1 (MQ=255)
                                                                                                                                            agGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa              >  2:58383/1‑150 (MQ=255)
                                                                                                                                            agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa              >  1:389883/1‑150 (MQ=255)
                                                                                                                                            agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa              >  1:396770/1‑150 (MQ=255)
                                                                                                                                             ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt             >  2:738760/1‑150 (MQ=255)
                                                                                                                                             ggTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt             >  2:55030/1‑150 (MQ=255)
                                                                                                                                                  cTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActa        >  1:884140/1‑149 (MQ=255)
                                                                                                                                                    tCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGTAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACtctc      <  2:1311847/150‑1 (MQ=255)
                                                                                                                                                    tCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACtctc      <  2:1237746/150‑1 (MQ=255)
                                                                                                                                                    tCCCCAGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACtctc      <  1:1354509/150‑1 (MQ=255)
                                                                                                                                                     cccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTca     <  2:1576160/150‑1 (MQ=255)
                                                                                                                                                       cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca   >  2:598566/1‑150 (MQ=255)
                                                                                                                                                       cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca   >  2:1763386/1‑150 (MQ=255)
                                                                                                                                                       cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTATGATATTCGTATTGTCTAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca   >  1:1640411/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
AATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACAC  >  NC_000913/2900173‑2900471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: