Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 265,573 G→A 7.6% intergenic (‑30/+47) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,5730GA 7.6% 351.9 / 11.1 118intergenic (‑30/+47)ykfF/ykfBCP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (57/51);  new base A (6/3);  total (63/55)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 120 total aligned reads displayed.

CAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAAC  >  NC_000913/265427‑265722
                                                                                                                                                  |                                                                                                                                                     
  gACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGcc                                                                                                                                                  >  1:1323407/1‑150 (MQ=255)
    cGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTg                                                                                                                                                <  1:1626473/150‑1 (MQ=255)
      gAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCt                                                                                                                                              <  2:1117610/150‑1 (MQ=255)
        aGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTAGCGCTTGTCTCCGGGTGAAAGGCCCCTGTGGCAGCAACACGGATTGGGTCATTTTCTGGCTCCTGATAAAAGAAAACCCCGGCCGCCTGGTAg                                                                                                                                            >  1:145328/1‑150 (MQ=255)
           gACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCGGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTg                                                                                                                                         >  1:735452/1‑150 (MQ=255)
           gACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTAGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTg                                                                                                                                         >  1:1074164/1‑150 (MQ=255)
                 ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCgggg                                                                                                                                   <  1:1304482/150‑1 (MQ=255)
                   tGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTg                                                                                                                                 <  2:1145924/150‑1 (MQ=255)
                   tGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTg                                                                                                                                 <  2:1078076/150‑1 (MQ=255)
                       cggcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAtt                                                                                                                             <  1:975464/147‑1 (MQ=255)
                        gtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAACTGTCGGGGTGAAttt                                                                                                                            >  1:1743929/1‑150 (MQ=255)
                         tcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTg                                                                                                                           >  1:1068752/1‑150 (MQ=255)
                          cTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGc                                                                                                                          >  2:1524383/1‑150 (MQ=255)
                          cTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTCGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTCTCGGGGTGAATTTGc                                                                                                                          >  2:1689052/1‑150 (MQ=255)
                             ggAGTGTGATATTGCTGTACGTGGTAGTGTCCGCTTGCGCTTGTCGCCGGGGGAAAGGCCCCGGTGGCAGCAACTCTGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTgg                                                                                                                       <  2:1428437/149‑1 (MQ=255)
                                gtgtGATATTGCTGTACGTGGTAGTGACCGCTTGCTCTTGTCTCCGGGTGAAAGGCCCCGGTGTCATCAAAACGGATTGCGTCAATTACGGGCTCCAGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGTGGa                                                                                                                    >  1:212124/1‑150 (MQ=255)
                                gtgtGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGa                                                                                                                    >  1:32443/1‑150 (MQ=255)
                                gtgtGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTAGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCAAGCTGTCGGGGTGAATTTGCTGGGGa                                                                                                                    >  2:914510/1‑150 (MQ=255)
                                  gtgATATTGCTGTACGTGGTAGTGACCGCTTGCGCATGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAg                                                                                                                  <  1:818499/150‑1 (MQ=255)
                                   tgATATTGCTGTACGTGGTAGTGCCCGCTTGCGCTTGTCTCCGTGTGAAATGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCATGATAAAAGAAAACCCCGGCAGCCTGCTAGATGTCGGGGTGAAGTTTCTGGGTAAGa                                                                                                                 >  1:403218/1‑150 (MQ=255)
                                   tgATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGTCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGa                                                                                                                 <  1:1675134/150‑1 (MQ=255)
                                    gATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAt                                                                                                                >  2:1749004/1‑150 (MQ=255)
                                     atatTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAta                                                                                                               >  1:1522735/1‑150 (MQ=255)
                                        ttGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                            >  2:1750199/1‑150 (MQ=255)
                                         tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtactac                                                                                                           >  2:1482863/1‑150 (MQ=255)
                                          gCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                             >  1:1140233/1‑147 (MQ=255)
                                          gCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                             <  2:1140233/147‑1 (MQ=255)
                                          gCTGCACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                             <  2:909403/147‑1 (MQ=255)
                                          gCTGCACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                             >  1:909403/1‑147 (MQ=255)
                                           cTGTACGTGGTAGTGACCGCTTGCGCTGGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATCGGGCCAGTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATActacta                                                                                                         <  1:138829/150‑1 (MQ=255)
                                            tGTACTTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGTCCTCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAt                                                                                                        <  2:174664/150‑1 (MQ=255)
                                            tGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAt                                                                                                        >  2:291218/1‑150 (MQ=255)
                                             gTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATc                                                                                                       <  1:785353/150‑1 (MQ=255)
                                              tACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCa                                                                                                      <  1:1540458/150‑1 (MQ=255)
                                                cGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGt                                                                                                    >  1:1073260/1‑150 (MQ=255)
                                                 gTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTc                                                                                                   >  1:882125/1‑150 (MQ=255)
                                                 gTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTc                                                                                                   >  1:519627/1‑150 (MQ=255)
                                                  tGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGATGGGGAAGATACTACTATCAGTCg                                                                                                  >  2:170432/1‑150 (MQ=255)
                                                    gTAGTTACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGtt                                                                                                <  1:394940/150‑1 (MQ=255)
                                                    gTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGtt                                                                                                <  2:1169749/150‑1 (MQ=255)
                                                      aGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgc                                                                                              <  1:1608442/150‑1 (MQ=255)
                                                      aGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgc                                                                                              <  1:209643/150‑1 (MQ=255)
                                                       gTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGGTAGCTGTCGGGGTGAATTTGCTGGGGAAGAAACTACTATCAGTCGTtgct                                                                                             >  1:1518160/1‑150 (MQ=255)
                                                         gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                           >  2:841774/1‑150 (MQ=255)
                                                         gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                           >  1:507113/1‑150 (MQ=255)
                                                         gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                           <  2:44073/150‑1 (MQ=255)
                                                           ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAg                                                                                         <  2:410653/150‑1 (MQ=255)
                                                                tgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTcc                                                                                    >  1:626913/1‑150 (MQ=255)
                                                                   gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCgag                                                                                 >  1:634296/1‑150 (MQ=255)
                                                                   gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCgag                                                                                 >  2:536819/1‑150 (MQ=255)
                                                                       gTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                             <  1:868807/150‑1 (MQ=255)
                                                                        tctcCGGGCGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTgg                                                                            <  1:1207861/150‑1 (MQ=255)
                                                                         ctcCGGGTGAAAGGCCCCGTTAGCAGCAACACGGATTGGTTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                           <  1:50883/150‑1 (MQ=255)
                                                                         ctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                           >  2:613128/1‑150 (MQ=255)
                                                                           ccGGGGGAACGGCCCCGGTGGCCGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCGGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACa                                                                         <  1:1381134/150‑1 (MQ=255)
                                                                             gggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGa                                                                       >  2:1714973/1‑150 (MQ=255)
                                                                              ggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCTGTCTCCGAGAGTGGACAGaa                                                                      >  2:1797601/1‑150 (MQ=255)
                                                                                tGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACt                                                                    >  1:1224054/1‑150 (MQ=255)
                                                                                      gCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCa                                                              <  2:230260/150‑1 (MQ=255)
                                                                                       ccccGGTGGCAGCAACACGGATTGGGTCATTTTCTGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGCGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAg                                                             >  2:1398305/1‑150 (MQ=255)
                                                                                       ccccGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAg                                                             <  1:1595195/150‑1 (MQ=255)
                                                                                         ccGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCg                                                           <  2:134235/150‑1 (MQ=255)
                                                                                            gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttc                                                        <  2:612438/150‑1 (MQ=255)
                                                                                               gcagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttcttc                                                     <  2:624123/150‑1 (MQ=255)
                                                                                                cagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGGTGCAGTCTCCGAGAGTGGCCAGAACTTTCTCAGCGTTCTTcc                                                    >  2:1246424/1‑150 (MQ=255)
                                                                                                               ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAgg                                     >  2:990861/1‑150 (MQ=255)
                                                                                                               ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAgg                                     <  2:1053610/150‑1 (MQ=255)
                                                                                                               ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAgg                                     >  1:925525/1‑150 (MQ=255)
                                                                                                                gTCATATTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGt                                    >  2:493022/1‑150 (MQ=255)
                                                                                                                 tCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTc                                   >  1:76346/1‑150 (MQ=255)
                                                                                                                    ttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTcccg                                >  2:1625102/1‑149 (MQ=255)
                                                                                                                     tttCGGGCTCCTGATAACAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCCCCGAGAGTGGACAGAACTTTCTCAGCGTGCTTCCGGTCCGCAGTGAAGGTCCCTg                               <  2:245998/150‑1 (MQ=255)
                                                                                                                       tCGGGCTCCTGATACAAGACAACACCGGCAGCCTGCTAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCt                             <  1:661091/150‑1 (MQ=255)
                                                                                                                             tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                       <  2:1219043/150‑1 (MQ=255)
                                                                                                                              ccTGATAACAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACTGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGt                      <  2:1198052/150‑1 (MQ=255)
                                                                                                                              ccTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGt                      <  1:1000496/150‑1 (MQ=255)
                                                                                                                                 gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAt                   >  1:1897687/1‑150 (MQ=255)
                                                                                                                                   tAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGGCCCTGCTTTGTGGTCATTg                 >  2:216402/1‑150 (MQ=255)
                                                                                                                                    aaaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGa                <  2:1698646/150‑1 (MQ=255)
                                                                                                                                     aaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc               >  2:556591/1‑150 (MQ=255)
                                                                                                                                     aaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCACAGTGAAGGTCCCTGCTTTGTGGTCATTGAc               >  1:1582366/1‑150 (MQ=255)
                                                                                                                                      aaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACg              >  2:129469/1‑150 (MQ=255)
                                                                                                                                      aaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACg              <  1:602306/150‑1 (MQ=255)
                                                                                                                                       aGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGt             <  2:620747/150‑1 (MQ=255)
                                                                                                                                        caaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTa            <  1:804187/149‑1 (MQ=255)
                                                                                                                                         aaaaCCCGGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGTGAGTGGACAGAACTTTCTCAGCGTTCTTCGGGTCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg           <  2:1518160/150‑1 (MQ=255)
                                                                                                                                         aaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGGCAGAACTTTCTCAGCGGTCTTCCGGTCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACGTat           >  2:942138/1‑149 (MQ=255)
                                                                                                                                         aaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg           <  2:1315441/150‑1 (MQ=255)
                                                                                                                                         aaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg           <  1:536819/150‑1 (MQ=255)
                                                                                                                                          tcaCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGa          >  1:695634/3‑150 (MQ=255)
                                                                                                                                          aaaCCCCGGCAGCCTGCTAGCGGGCGGGGTGAATGTGCTGGGGAAGAAACTACTATCAGGCGTTGCGGCAGTCGCCGAGAGTGGACAGAACTTTCTCAGCGTTCATCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGa          <  2:225301/150‑1 (MQ=255)
                                                                                                                                            aCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACg        >  1:435373/1‑150 (MQ=255)
                                                                                                                                            aCCCCGGCAGCCTGCTAGCTCTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGAGAAGGTCCCTGCTTTGTGGTCAtagacgtagacg        >  1:463878/1‑150 (MQ=255)
                                                                                                                                             ccccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATAATACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt       >  2:1257139/1‑150 (MQ=255)
                                                                                                                                             ccccGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt       >  1:488240/1‑150 (MQ=255)
                                                                                                                                             ccccGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt       >  2:1756872/1‑150 (MQ=255)
                                                                                                                                              cccGTCAGCCTGCTAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTc      <  1:914510/150‑1 (MQ=255)
                                                                                                                                               ccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg     <  2:1019647/150‑1 (MQ=255)
                                                                                                                                                  gCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAAc  <  2:735452/150‑1 (MQ=255)
                                                                                                                                                  gCAGCCGGCTAGCTGTCGGGGTGAATTTGCTGTGGAAGATACTACTATCAGTCGTTGCGGCAGTCTCCGAGAGTTGACAGAACTTTCTCAGCGGTCTTCCGGTCCGCAGGGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAAc  <  1:1739929/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAAC  >  NC_000913/265427‑265722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: