Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,327 A→C 5.2% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3270AC 5.2% 429.3 / 10.2 146intergenic (‑54/‑265)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (69/68);  new base C (4/4);  total (74/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 151 total aligned reads displayed.

TGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAAC  >  NC_000913/2900179‑2900474
                                                                                                                                                       |                                                                                                                                                   
tGGCCTAAACCGCTATTCCCACCGGTAACAAGTGCGGTTTTACCTTTCAGGGAGATAAAATCCTTTGAGAACGCATGGAGAGCATCGAATGCCATAGTTAGATCCAAATTCAGTAAGGC‑‑‑AAAAAAAAAAAAAGGTAACTTCCCCCgagat                                                                                                                                                    >  2:1034569/1‑149 (MQ=255)
 ggCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                                                                                                                   >  2:1464506/1‑150 (MQ=255)
 ggCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                                                                                                                   <  1:394883/150‑1 (MQ=255)
   gcTAAACAGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGTATAAAAAATCCATTGATAACGCATTGAGACATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCA‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                                                                                                                  <  2:417692/149‑1 (MQ=255)
   ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                                 >  2:236366/1‑150 (MQ=255)
   ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                                 >  1:1565279/1‑150 (MQ=255)
       aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCGGGGAGAAAAAATCCATTGCGAACGCATTGAGAGATTAGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGAGGTAGGGAAg                                                                                                                                             >  2:89758/1‑150 (MQ=255)
            cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTAcc                                                                                                                                        <  2:1568769/150‑1 (MQ=255)
             tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                       <  2:1353533/150‑1 (MQ=255)
             tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcca                                                                                                                                       >  1:140473/1‑149 (MQ=255)
             tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCt                                                                                                                                       >  1:1888959/1‑150 (MQ=255)
             tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATGGCGAGATTCGATTGACATAGTTAGCTCCAATTTCAGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                       <  2:1346992/150‑1 (MQ=39)
              aTTCCCCACGGTAACTATTTCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAGCTTCCCTCTCGCGGGGGAAGTTACCtt                                                                                                                                      <  1:165384/150‑1 (MQ=255)
              aTTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                                                                                                      <  1:1217101/150‑1 (MQ=255)
                tCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                                                                                                    <  2:552262/150‑1 (MQ=255)
                  ccACCGGTAACAATTGCGTTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTTACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                                  <  1:1768207/150‑1 (MQ=255)
                  ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGATATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                                  <  1:787473/150‑1 (MQ=255)
                  ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                                  <  2:1109022/150‑1 (MQ=255)
                  ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGAGAGAGGGAAGTTACCTTtatc                                                                                                                                  >  2:1276601/1‑150 (MQ=255)
                   cACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCa                                                                                                                                 <  2:620307/150‑1 (MQ=255)
                      cGGTAACAATTGCGGTTTTACCTTTCAGGTAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTa                                                                                                                              <  2:1160185/150‑1 (MQ=255)
                           aCAATTGCGGTTTTACCTTTCCGGCACAAAAAATCCATTGACACCCCATTCACAGATTCTATTCACATAGTAACCTCCAATTTCCGTAAGCA‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATctct                                                                                                                         <  1:495293/150‑1 (MQ=255)
                            cAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTg                                                                                                                        >  1:887310/1‑150 (MQ=255)
                             aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                       <  2:609428/150‑1 (MQ=255)
                               ttGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                                                                     >  2:668374/1‑150 (MQ=255)
                                tGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTaa                                                                                                                    <  2:1772408/150‑1 (MQ=255)
                                    gTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                                <  2:1565279/150‑1 (MQ=255)
                                           cTTTCAGGGATAAAAAATCCATTGAGAAAGCATTGATAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                                                                                                            <  2:640428/150‑1 (MQ=255)
                                           cTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAt                                                                                                         >  1:1110879/1‑150 (MQ=255)
                                             ttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt                                                                                                       >  2:672566/1‑150 (MQ=255)
                                               aaGGGAGAAAAAATTCATTGAGAACGCATTGAGAGATTCGATTTACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAATATAGGTAACTTCCCCCGCGACAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttttt                                                                                                     <  1:260695/149‑1 (MQ=255)
                                                 gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                   <  1:1000323/150‑1 (MQ=255)
                                                 gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                   <  2:906038/150‑1 (MQ=255)
                                                    agaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt                                                                                                >  2:187729/1‑150 (MQ=255)
                                                    agaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCACATTATCTATGGTAACTGGAGATAAATTTTAGCAt                                                                                                >  1:1725356/1‑150 (MQ=255)
                                                        aaaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg                                                                                            >  2:1652987/1‑150 (MQ=255)
                                                                ttGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACt                                                                                    <  1:936641/150‑1 (MQ=255)
                                                                ttGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACt                                                                                    <  1:192953/150‑1 (MQ=255)
                                                                 tGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg                                                                                   >  1:1204859/1‑150 (MQ=255)
                                                                   agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGT‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCt                                                                                 <  1:707426/150‑1 (MQ=255)
                                                                   agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCt                                                                                 <  2:966252/150‑1 (MQ=255)
                                                                     aaCGCATTGGGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTaa                                                                               <  1:1317451/150‑1 (MQ=255)
                                                                     aaCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTaa                                                                               >  2:784902/1‑150 (MQ=255)
                                                                        gCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTGACCTTTCTCATTATCTATGGTAACTGGAGATAATTTTTAGCATAAGTGCATAACTGCTAAtgt                                                                            >  2:1245506/1‑150 (MQ=255)
                                                                         cATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                                                                           >  1:799231/1‑150 (MQ=255)
                                                                         cATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                                                                           <  1:1610254/150‑1 (MQ=255)
                                                                           ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCAGTAAGTA‑‑‑AAAAAAAAAGAAATGTAACTTCCCCCGCGAGAGTGAAGTTACCTTTCTCATTATCTCTGGTAACTGGATATAATTTTTAGCATAAGGGCATAACTGCTAAtgtgtt                                                                         <  1:674962/150‑3 (MQ=255)
                                                                            tGAGATATTCGATAGACATAGTTAGCCCCAATTTCCGTAAGGC‑‑‑AAAAAAAACGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTAATTATCTCTGGTTACTGTAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                        <  2:47579/150‑1 (MQ=255)
                                                                               gagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCCTTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTa                                                                     >  2:1201976/1‑150 (MQ=255)
                                                                               gagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGAATAAGGGCATAACTGCTAATGTGATCGTa                                                                     >  1:241020/1‑150 (MQ=255)
                                                                                cgaTTCGCTTGACATCTTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTaa                                                                    <  2:1663413/149‑1 (MQ=255)
                                                                                agaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTaa                                                                    >  2:1415766/1‑150 (MQ=255)
                                                                                 gaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAACAGAAAGGTAACTTCCACCGATAGAGTGAAGTTCCCTTTCTCATTAACTCTTGTAACTGGAGATAATTTTTCGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                   >  1:106872/1‑150 (MQ=255)
                                                                                  aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATc                                                                  >  1:1771828/1‑150 (MQ=255)
                                                                                    tCGATTGACATAGTTATCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcac                                                                <  2:123844/150‑1 (MQ=255)
                                                                                    tCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcac                                                                <  2:1625728/150‑1 (MQ=255)
                                                                                      gATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAg                                                              >  2:1034435/1‑150 (MQ=255)
                                                                                        ttGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATTATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg                                                            >  2:366002/1‑150 (MQ=255)
                                                                                          taCATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTACTCACAGTGTGa                                                         <  1:995250/149‑1 (MQ=255)
                                                                                          gACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                          <  2:1223811/150‑1 (MQ=255)
                                                                                            cATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTATCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                        >  1:511793/1‑150 (MQ=255)
                                                                                            aaTAGTTAGCTCCAATTTCCGTAATGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                        <  1:437923/149‑1 (MQ=255)
                                                                                              tAGTTAGCTCCAATTTCCATAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGatat                                                      >  1:1258068/1‑150 (MQ=255)
                                                                                                 ttAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCg                                                   <  1:236366/150‑1 (MQ=255)
                                                                                                    gCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                                <  1:520823/150‑1 (MQ=255)
                                                                                                    gCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAt                                                >  1:81165/1‑150 (MQ=255)
                                                                                                     cTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                               >  1:1542696/1‑150 (MQ=255)
                                                                                                         aaTTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCa                                           >  1:617632/1‑150 (MQ=255)
                                                                                                             tCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                       >  1:750798/1‑150 (MQ=255)
                                                                                                               cGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGAAATCACAGTGTGATATTCGTATTGTCAaataat                                     >  2:122770/1‑150 (MQ=255)
                                                                                                                gTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                    >  1:609702/1‑150 (MQ=255)
                                                                                                                gTAAGGA‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                    <  1:1322708/150‑1 (MQ=255)
                                                                                                                   aGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTa                                 >  2:499048/1‑150 (MQ=255)
                                                                                                                   aGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTa                                 <  2:1461671/150‑1 (MQ=255)
                                                                                                                    ggC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaa                                >  2:242505/1‑150 (MQ=255)
                                                                                                                     gC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                               >  2:610446/1‑150 (MQ=255)
                                                                                                                      c‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAGCTTTCTCATAATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                              >  2:293751/1‑150 (MQ=255)
                                                                                                                      c‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                              <  1:146492/150‑1 (MQ=255)
                                                                                                                      c‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                              >  2:1864369/1‑150 (MQ=255)
                                                                                                                           aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                            <  2:1763627/150‑1 (MQ=255)
                                                                                                                           aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                            <  2:1814800/150‑1 (MQ=255)
                                                                                                                           aaaaaaaaGAAACGTAACATCCCCCGCGACATGGAAGTTACCTTTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCATATGGGCATAACTGCTAATGTGATCGTAATCACAGGGTGATATTCGTATTGTCAAATAATTTTAAAAAg                            <  2:312734/150‑1 (MQ=255)
                                                                                                                             aaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACGTTTCGCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGATAATGTGATCGTAATCACAGTGTGATATTGGTATTGTCAAATAATTTTAAAAAGCa                          >  1:215455/1‑150 (MQ=255)
                                                                                                                              aaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAGCTTTCTCATTATCTCTTGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAg                         <  2:1412410/150‑1 (MQ=255)
                                                                                                                              aaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAg                         <  2:1600147/150‑1 (MQ=255)
                                                                                                                               aaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGa                        <  2:814173/150‑1 (MQ=255)
                                                                                                                                 aagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAg                      >  1:128002/1‑150 (MQ=255)
                                                                                                                                 aagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAg                      <  1:1345585/150‑1 (MQ=255)
                                                                                                                                      agGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa                 >  2:58383/1‑150 (MQ=255)
                                                                                                                                      agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa                 >  1:389883/1‑150 (MQ=255)
                                                                                                                                        gTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAtt               >  2:1234233/1‑150 (MQ=255)
                                                                                                                                         caaCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGGCAAATAATTTTAAAAAGCAGAAGCGTGATTa              >  2:127825/2‑150 (MQ=255)
                                                                                                                                            cTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActa           >  1:884140/1‑149 (MQ=255)
                                                                                                                                            cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc           >  1:1624656/1‑150 (MQ=255)
                                                                                                                                              tCCCCAGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACtctc         <  1:1354509/150‑1 (MQ=255)
                                                                                                                                               cccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTca        >  1:1071153/1‑150 (MQ=255)
                                                                                                                                                 cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca      >  2:598566/1‑150 (MQ=255)
                                                                                                                                                 cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca      <  2:1110879/150‑1 (MQ=255)
                                                                                                                                                     cgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACacaac  <  2:551424/150‑1 (MQ=255)
                                                                                                                                                     cgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTACCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACacaac  <  2:1791165/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
TGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAAC  >  NC_000913/2900179‑2900474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: