BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,907,487	T→A	6.6%	N152I (AAC→ATC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,487	0	T	A	6.6%	377.7 / 12.5	131	N152I (AAC→ATC)	eno	enolase
Reads supporting (aligned to +/- strand): ref base T (56/65); new base A (2/7); total (58/73)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 134 total aligned reads displayed.

TGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTG  >  NC_000913/2907338‑2907636

tGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGt                                                                                                                                                       >  2:383894/1‑150 (MQ=255)

   cTTTCGCTTTCAGAAGTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtca                                                                                                                                                    <  1:1454730/150‑1 (MQ=255)

   cTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtca                                                                                                                                                    <  1:19194/150‑1 (MQ=255)

       cGCTTTCAGAACTATTGCCAGGTGATGGAAAACTTCAGAACCCATGAGGATGGCTTCTTTCACAGTTTTCGCGACAACCGGCTGAATCATGAATTCCTTGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTcatcat                                                                                                                                                <  1:27553/150‑1 (MQ=255)

        gCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATAAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCatcatc                                                                                                                                               >  1:1744275/1‑150 (MQ=255)

         cTTTCAGAACTTTTTTCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCATCGTGCTCACCACCGTTGATGATGTTCATCATCg                                                                                                                                              <  2:423615/150‑1 (MQ=255)

          tttCAGAACTTTTGCCATGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCgg                                                                                                                                             <  2:781729/150‑1 (MQ=255)

          tttCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGAACATCATCAACgg                                                                                                                                             <  2:480777/150‑1 (MQ=255)

            tCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCGTGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGaa                                                                                                                                           >  1:1889091/1‑150 (MQ=255)

             cAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAAc                                                                                                                                          >  1:1638605/1‑150 (MQ=255)

             cAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAAc                                                                                                                                          >  2:417081/1‑150 (MQ=255)

             cAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGAACATCATCAACggtgg                                                                                                                                          <  1:12105/150‑4 (MQ=255)

             cAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATAGCAACGTTGTTGTCAGCGTGCTCACCACCGGTGATGATGAACatcatcaacgg                                                                                                                                          <  1:743193/150‑6 (MQ=255)

              aGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGAACatcatcaacggt                                                                                                                                         <  1:1112852/150‑7 (MQ=255)

               gAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCg                                                                                                                                        >  2:1718810/1‑150 (MQ=255)

               gAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGATGATGAACATCATCAACggtggtg                                                                                                                                        <  1:900455/150‑6 (MQ=255)

                aaCTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCgg                                                                                                                                       <  1:855891/150‑1 (MQ=255)

                 aCTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTTAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGc                                                                                                                                      <  2:1068149/150‑1 (MQ=255)

                 aCTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGc                                                                                                                                      >  2:47776/1‑150 (MQ=255)

                  cTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCa                                                                                                                                     <  2:763552/150‑1 (MQ=255)

                  cTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCa                                                                                                                                     <  1:795811/150‑1 (MQ=255)

                   ttttTCCAGGTGATGGAAAACTTAAGTTCCGATGCGGACGTCTTCTTTGACAGTTTTCGCGCAAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGATCCGGCAt                                                                                                                                    <  2:1197089/150‑1 (MQ=255)

                   ttttGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAt                                                                                                                                    <  2:610707/150‑1 (MQ=255)

                    tttGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACAGTTGATGATGTTCATCATCGGAACCGGCATa                                                                                                                                   >  1:1258947/1‑150 (MQ=255)

                     ttGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATag                                                                                                                                  <  1:47776/150‑1 (MQ=255)

                      tGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATaga                                                                                                                                 >  2:1363400/1‑150 (MQ=255)

                       gCCAGGTGATGGAAAACTTCAGAACCCATGCGGATTGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATagag                                                                                                                                <  2:1124988/150‑1 (MQ=255)

                          aGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTAt                                                                                                                             >  1:373365/1‑150 (MQ=255)

                               aTGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGcc                                                                                                                        <  2:9620/150‑1 (MQ=255)

                                tGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGccc                                                                                                                       >  1:1035954/1‑150 (MQ=255)

                                tGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGccc                                                                                                                       >  2:424576/1‑150 (MQ=255)

                                tGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGccc                                                                                                                       >  1:972440/1‑150 (MQ=255)

                                tGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGccc                                                                                                                       >  1:1217631/1‑150 (MQ=255)

                                 ggAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCTTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCg                                                                                                                      <  2:1766015/150‑1 (MQ=255)

                                  gAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCgg                                                                                                                     >  1:596604/1‑150 (MQ=255)

                                       cTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAc                                                                                                                <  1:521401/150‑1 (MQ=255)

                                        ttCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAcc                                                                                                               <  1:606010/150‑1 (MQ=255)

                                            gAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttc                                                                                                           >  1:1692780/1‑150 (MQ=255)

                                            gAACCCATGCGGATGGATTCTTTCACAGTTTTCGCGCGAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCCTCATCGGAACCGGCATAGAGTTTTTGCCCGGAGTACCgttc                                                                                                           <  2:383644/150‑1 (MQ=255)

                                            caaCCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttc                                                                                                           <  1:1782942/149‑1 (MQ=255)

                                              aCCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcag                                                                                                         >  2:1524233/1‑150 (MQ=255)

                                                  aTGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagttca                                                                                                     <  2:1008687/150‑1 (MQ=255)

                                                   tGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATGAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGttcagttcag                                                                                                    <  1:403271/150‑1 (MQ=255)

                                                   tGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGttcagttcag                                                                                                    >  2:159393/1‑150 (MQ=255)

                                                   tGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGttcagttcag                                                                                                    <  1:1013933/150‑1 (MQ=255)

                                                    gCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagttcctc                                                                                                   >  2:296756/1‑147 (MQ=255)

                                                        ttGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGAtg                                                                                               <  1:1655843/149‑1 (MQ=255)

                                                        aTGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGAtg                                                                                               <  1:1140049/150‑1 (MQ=255)

                                                        aTGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGAtg                                                                                               <  2:602326/150‑1 (MQ=255)

                                                            cttcttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCATCGATGTGCt                                                                                           >  1:133957/1‑150 (MQ=255)

                                                              tcttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg                                                                                         <  1:252148/150‑1 (MQ=255)

                                                                 ttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc                                                                                      <  1:1176861/150‑1 (MQ=255)

                                                                 ttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc                                                                                      >  1:1813900/1‑150 (MQ=255)

                                                                     caGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCg                                                                                  >  2:1131725/1‑150 (MQ=255)

                                                                        ttttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa                                                                               <  1:1206909/150‑1 (MQ=255)

                                                                         tttCGCGCCACCCGGCTGAATCATGAATTCCTGGATCTCAACGTTGGTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCAt                                                                              <  2:748207/150‑1 (MQ=255)

                                                                         tttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGATTACCGTTGAGTTCAGCGATGTGCTCGTACAGCGGCAt                                                                              >  1:360468/1‑150 (MQ=255)

                                                                           tCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATAc                                                                            <  2:1562295/150‑1 (MQ=255)

                                                                              cgcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCCCCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCAGCCCtt                                                                         >  1:105049/1‑150 (MQ=255)

                                                                               gcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCttt                                                                        >  2:822246/1‑150 (MQ=255)

                                                                               gcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCttt                                                                        <  2:1306986/150‑1 (MQ=255)

                                                                                ccAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTa                                                                       >  1:1158609/1‑150 (MQ=255)

                                                                                  aaCCGGCTGAATGATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGTTGATGTTCTTCATCGGAAACGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTGAGCGATGTGCTCGTACAGCGGCATACCTTTagc                                                                     <  1:1834764/150‑1 (MQ=255)

                                                                                  aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagc                                                                     <  1:1808754/150‑1 (MQ=255)

                                                                                  aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGGGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagc                                                                     <  1:1852876/150‑1 (MQ=255)

                                                                                   aCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagca                                                                    <  2:1683173/150‑1 (MQ=255)

                                                                                      ggCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCt                                                                 >  1:984659/1‑150 (MQ=255)

                                                                                        cTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTgc                                                               <  2:839183/150‑1 (MQ=255)

                                                                                        cTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTgc                                                               <  1:231071/150‑1 (MQ=255)

                                                                                            aTCTTGAATTCCTGGATATCAACGTTGTTGTCAGCGTGATCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGCAGTCCCGTTGAGTTCAGCGATGTGCTCGTACCGCTGCAGACCATTAGCAGCTgcagcc                                                           >  2:235133/1‑149 (MQ=255)

                                                                                                 gAATTCCTTGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCttt                                                      <  1:930851/150‑1 (MQ=255)

                                                                                                   aTTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGATTACCGTTCAGTTCAGCGAGGTCCTCGTAAAGCGGCATACATTTAGCAGAGGCCGCAGCTTTgg                                                    >  1:1432253/1‑150 (MQ=255)

                                                                                                     tCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg                                                  <  2:972440/150‑1 (MQ=255)

                                                                                                     tCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg                                                  <  1:772996/150‑1 (MQ=255)

                                                                                                       cTGGATATCAACGTTGGTGTCAGCGTGCTCACCACCGTTGATGATGGTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTAAGCAGCTGCAGCAGCTTTGGCGtt                                                <  1:1180318/150‑1 (MQ=255)

                                                                                                         ggATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAg                                              <  1:1569095/150‑1 (MQ=255)

                                                                                                         ggATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAg                                              >  2:229803/1‑150 (MQ=255)

                                                                                                           atatCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGcc                                            <  2:1294904/150‑1 (MQ=255)

                                                                                                             atCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCag                                          >  2:894899/1‑150 (MQ=255)

                                                                                                              tCAACGTTGTTGTCAGCTTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCaga                                         <  1:947351/150‑1 (MQ=255)

                                                                                                               cAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCagag                                        >  2:486384/1‑150 (MQ=255)

                                                                                                               cAACGTAGTTGTCAGCGAGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATATACAATTTGCCAGGTGTAGCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCATTCGCAGCTGCAGCAGCTTAGGAGTTAGCCacat                                        >  2:1355166/1‑147 (MQ=255)

                                                                                                                aaCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAgaga                                       <  2:1889091/150‑1 (MQ=255)

                                                                                                                 aCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCATCTGCAGCAGCTTTGGCGTTAGCCAGAGAt                                      <  2:1243311/150‑1 (MQ=255)

                                                                                                                  cggtgttgtCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTCCAGCAGCTTTGGCGTTAGCCAGAGATa                                     <  1:954746/147‑1 (MQ=255)

                                                                                                                  cGTTGTTGTCATCGTGCTCACCACCGTTGATGATGATCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATa                                     >  2:734647/1‑150 (MQ=255)

                                                                                                                  cGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATa                                     >  2:1189995/1‑150 (MQ=255)

                                                                                                                  cGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATa                                     <  2:1236700/150‑1 (MQ=255)

                                                                                                                    ttgttgTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCATCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACa                                   <  2:133957/150‑1 (MQ=255)

                                                                                                                        tgtCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCa                               >  2:1906275/1‑150 (MQ=255)

                                                                                                                           cAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGa                            >  1:1699103/1‑150 (MQ=255)

                                                                                                                            aGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                           >  1:413106/1‑150 (MQ=255)

                                                                                                                            aGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGAt                           >  1:599845/1‑150 (MQ=255)

                                                                                                                                  cTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGtt                     >  1:455840/1‑150 (MQ=255)

                                                                                                                                  cTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGtt                     >  2:252674/1‑150 (MQ=255)

                                                                                                                                  cTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCAAGAGATACAGCCAGGATTGCGtt                     >  1:521580/1‑150 (MQ=255)

                                                                                                                                      ccaccGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGCGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTcgcg                 >  1:1162428/1‑150 (MQ=255)

                                                                                                                                        accGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGcc               <  1:1347527/150‑1 (MQ=255)

                                                                                                                                              gatgatGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAAttt         >  1:1422854/1‑150 (MQ=255)

                                                                                                                                                tgatgTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTgg       >  2:1846438/1‑150 (MQ=255)

TGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCAGGATTGCGTTCGCGCCGAATTTGGATTTG  >  NC_000913/2907338‑2907636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`