Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,105,533 G→C 5.2% intergenic (+21/‑181) mltC → / → nupG membrane‑bound lytic murein transglycosylase C/nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,5330GC 5.2% 338.0 / 7.6 110intergenic (+21/‑181)mltC/nupGmembrane‑bound lytic murein transglycosylase C/nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base G (43/60);  new base C (3/3);  total (46/64)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 112 total aligned reads displayed.

GCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAAC  >  NC_000913/3105384‑3105681
                                                                                                                                                     |                                                                                                                                                    
gCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACg                                                                                                                                                      <  2:690144/150‑1 (MQ=255)
gCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACg                                                                                                                                                      >  2:1547397/1‑150 (MQ=255)
     tattatTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACACGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCt                                                                                                                                                 >  1:959875/1‑150 (MQ=255)
          ttaACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAggg                                                                                                                                            >  1:642876/1‑150 (MQ=255)
          ttaACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAggg                                                                                                                                            <  2:734045/150‑1 (MQ=255)
           taACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCACCTGACGATTCTCAgggg                                                                                                                                           >  1:160180/1‑150 (MQ=255)
             acacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAGCATCGTCAGGGGcg                                                                                                                                         <  1:1485696/150‑2 (MQ=255)
             acacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAGCATCGTCAGGGGcg                                                                                                                                         <  1:1709646/150‑2 (MQ=255)
             acacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCa                                                                                                                                         >  1:678216/1‑150 (MQ=255)
              cacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTATGCGGAATCTCGCCGTTATCTTTATAAACTGAATACCGCGCAAAAATCCTACCGCCCCCCATAATTCCATTAACCGCCCCTGCCGATGCTCAGGGGCaa                                                                                                                                        >  1:442170/1‑150 (MQ=255)
               acaaTGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCAGATAATTCCAATAACAGCCCCTGAACATGCTCAGGGGCaaa                                                                                                                                       >  1:1202243/4‑150 (MQ=255)
               acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTGCCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCata                                                                                                                                       >  2:707323/1‑148 (MQ=255)
               acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaa                                                                                                                                       <  2:1865966/150‑1 (MQ=255)
               acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACAGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaa                                                                                                                                       >  1:1408000/1‑150 (MQ=255)
               acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCACCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAGCATCGTCAGGGGcggt                                                                                                                                       <  1:1866428/150‑4 (MQ=255)
                 cATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaaaa                                                                                                                                     <  2:1134096/150‑1 (MQ=255)
                  aTGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAAt                                                                                                                                    <  2:736030/150‑1 (MQ=255)
                   tGACGCCGGGTGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATg                                                                                                                                   <  1:154988/150‑1 (MQ=255)
                   tGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATg                                                                                                                                   <  2:900928/150‑1 (MQ=255)
                    gACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCAGTTACCGCCCCTGCCGATGCTCAGGGGCAAAAATGt                                                                                                                                  >  2:1079980/1‑150 (MQ=255)
                      cGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTa                                                                                                                                <  1:1074357/150‑1 (MQ=255)
                         cGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGGTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcc                                                                                                                             <  1:1378560/150‑1 (MQ=255)
                         cGGGCGACGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAATTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATcc                                                                                                                             <  1:1198885/150‑1 (MQ=255)
                                    tATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAAtt                                                                                                                  >  1:1837514/1‑150 (MQ=255)
                                      tCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTc                                                                                                                >  1:1480365/1‑150 (MQ=255)
                                       cAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCTGGGGCAAAATTGTTATCCACATCAAAATTTCg                                                                                                               >  2:1040815/1‑150 (MQ=255)
                                          aCGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGttt                                                                                                            <  1:372691/150‑1 (MQ=255)
                                            gCTGACGACCCGCCATCCCTCTGCGGAATCTCTCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTg                                                                                                          <  2:1774310/150‑1 (MQ=255)
                                            gCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTCTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTg                                                                                                          <  1:272515/150‑1 (MQ=255)
                                            gCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTg                                                                                                          <  1:1116658/150‑1 (MQ=255)
                                              tGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCa                                                                                                        <  1:108534/150‑1 (MQ=255)
                                              tGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATATTTATAAAGGGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCa                                                                                                        <  2:1609940/150‑1 (MQ=255)
                                               gacgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                                                                                                       >  1:932562/1‑150 (MQ=255)
                                               gacgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                                                                                                       <  2:1733829/150‑1 (MQ=255)
                                                acgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCATAATTTCGTTTTGCaaa                                                                                                      <  2:1391644/150‑1 (MQ=255)
                                                 cgacCCGCCATCCATCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAt                                                                                                     >  1:638229/1‑150 (MQ=255)
                                                     ccGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCTCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTggg                                                                                                 <  2:1444487/150‑1 (MQ=255)
                                                         cATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATg                                                                                             >  1:1624334/1‑150 (MQ=255)
                                                              ctctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGc                                                                                        <  1:752251/150‑1 (MQ=255)
                                                              ctctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACAATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGc                                                                                        <  2:739069/150‑1 (MQ=255)
                                                               tctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCa                                                                                       <  2:219662/150‑1 (MQ=255)
                                                                 tGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGCGGCAAACATGTTATCCACAACACAAATTCGTTTTGCAAATTGGGAATGTTTGCAat                                                                                     >  2:909302/1‑150 (MQ=255)
                                                                   cGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAatta                                                                                   <  1:750400/150‑1 (MQ=255)
                                                                     taaTCTCGCCGTTATATATATAAAGTGAATACCGCGCAAAAATCCTACCGCAGCCGATAATTCAATTAAAATCACCTGACGATGCTCATTGTCAAAAATTTTATCCACTTCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAattatt                                                                                 <  1:57807/149‑1 (MQ=255)
                                                                       aTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCTATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTg                                                                               <  1:202947/150‑1 (MQ=255)
                                                                       aTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTg                                                                               <  1:693791/150‑1 (MQ=255)
                                                                        tctctcCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGc                                                                              <  2:1429476/150‑1 (MQ=255)
                                                                        tctcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGc                                                                              <  1:1251155/150‑1 (MQ=255)
                                                                          tcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCCCAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCca                                                                            >  2:1848464/1‑150 (MQ=255)
                                                                           cGCCGTTATCTTTATAAAGAGAATACCGCGCAAAAATCCTACCGCCTCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCcac                                                                           <  1:77102/150‑1 (MQ=255)
                                                                             ccGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAg                                                                         >  1:640337/1‑150 (MQ=255)
                                                                              cGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAgg                                                                        <  2:1064140/150‑1 (MQ=255)
                                                                              cGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACATCCCCTGACGATGCTCAGCGGCAAAAATGTTATCCACATCACAAATTCGATTTGCAAATTGGGAAAGTTTGCAATTATTTGCCAGAgg                                                                        >  1:139450/1‑150 (MQ=255)
                                                                                   tCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACa                                                                   <  1:1495523/150‑1 (MQ=255)
                                                                                     tttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaa                                                                 >  2:1540733/1‑150 (MQ=255)
                                                                                     tttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCACTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGGAATTATTTGCCACAGGTAACaaa                                                                 >  1:220590/1‑150 (MQ=255)
                                                                                      ttATAAAGTGAATACCGCTCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaaa                                                                <  2:1408000/150‑1 (MQ=255)
                                                                                      ttATAAAGTGAATACCGAGCAAAAATCCTACCGCCTCCGATAATTCCATTAACCGCCCCTGACGATGATCAGGGTCAAAAATTTTATCCACATCACAATTTAGTTTTGCAAATTGTGAATGTTTGCAATTATTTGCCACAGGTAACaaaa                                                                <  2:1202243/150‑1 (MQ=255)
                                                                                        ataAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTATGCAAATTCGGAATGTTTGCAATTATTTGCCAAAGGTAACaaaaaa                                                              >  2:1289378/1‑150 (MQ=255)
                                                                                         taAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGTTAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAc                                                             >  2:782559/1‑150 (MQ=255)
                                                                                         taAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAc                                                             >  1:358121/1‑150 (MQ=255)
                                                                                          aaaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                            <  2:1277123/150‑1 (MQ=255)
                                                                                          aaaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                            <  2:1540638/150‑1 (MQ=255)
                                                                                          aaaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                            <  1:341886/150‑1 (MQ=255)
                                                                                            aGTGAATACCGCGCAAAACTCCTACCGCCGCCTATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAg                                                          <  2:212156/150‑1 (MQ=255)
                                                                                              tGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTc                                                        <  1:1520091/150‑1 (MQ=255)
                                                                                                  aaCCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACAGTCCGCGa                                                   >  1:1022511/2‑150 (MQ=255)
                                                                                                    ccGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGaa                                                  >  1:4525/1‑150 (MQ=255)
                                                                                                        gcAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTg                                              <  2:959875/150‑1 (MQ=255)
                                                                                                         cAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGa                                             >  1:1452959/1‑150 (MQ=255)
                                                                                                         cAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTAATTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGa                                             >  1:1713336/1‑150 (MQ=255)
                                                                                                          aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGAt                                            <  1:357203/150‑1 (MQ=255)
                                                                                                          aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGAt                                            <  2:946363/150‑1 (MQ=255)
                                                                                                          aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGAt                                            <  2:1852489/150‑1 (MQ=255)
                                                                                                           aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGCAGTTGATa                                           >  1:1679297/1‑150 (MQ=255)
                                                                                                           aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATa                                           <  2:1480365/150‑1 (MQ=255)
                                                                                                           aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATa                                           <  2:650968/150‑1 (MQ=255)
                                                                                                            aaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAg                                          <  2:1528340/150‑1 (MQ=255)
                                                                                                            aaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAg                                          <  2:1814465/150‑1 (MQ=255)
                                                                                                                 cTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATcc                                     <  2:248314/150‑1 (MQ=255)
                                                                                                                      gccgccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCcatca                                <  2:670822/150‑1 (MQ=255)
                                                                                                                               aaTTCCATTAACCGACCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACg                       <  2:974754/150‑1 (MQ=255)
                                                                                                                                aTTCCATTAACAGCCACTGTAGATGCTCAGGGGCAATAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACgg                      <  1:122949/150‑1 (MQ=255)
                                                                                                                                 ttCCATTAACCGCCCCTGCCGATGCTCAGGGGCAAAAATGTTATCCACATCAAAATTTCGTTTTGCAAATTGGGAATTTTTGCAATTATTTTCCACATGTAACAAAAAAACAGTCCGCGAATTTGATAGAATCCCATAATCTCTCACGGt                     >  2:978856/1‑150 (MQ=255)
                                                                                                                                    cATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTACCAAAAACCCAGTCGGCGAAGTTGATAGAATCCCATCATATCGCACGGTCaa                  >  2:541814/1‑150 (MQ=255)
                                                                                                                                    cATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATACAATCATCTCGCACGGTCaa                  >  1:902436/1‑150 (MQ=255)
                                                                                                                                       tAACCGCCCCTGACGATGCTCAGGGGCAAATATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtg               <  2:442170/150‑1 (MQ=255)
                                                                                                                                       tAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtg               <  1:1185674/150‑1 (MQ=255)
                                                                                                                                        aaCCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgt              >  2:393145/1‑150 (MQ=255)
                                                                                                                                         aCCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg             >  1:926044/1‑150 (MQ=255)
                                                                                                                                         aCCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg             >  1:1814473/1‑150 (MQ=255)
                                                                                                                                         aCCGCCCCTGACGATGCTCAGGGGCAAAAATGATATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg             >  2:1099176/1‑150 (MQ=255)
                                                                                                                                           tgCCCCTGAGCATCGTCAGGGGCAAAAATGTTATCCACCTCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGCATCCCATCATCTCGCACGGTCAAATGTGCt           >  2:305844/2‑150 (MQ=255)
                                                                                                                                           tgCCCCTGAGCATCGTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCt           >  2:409378/2‑150 (MQ=255)
                                                                                                                                           cGCCCCAGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTTCAAATTGGGAATGTTTGCAATTATTAGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCt           <  2:1756108/150‑1 (MQ=255)
                                                                                                                                            gCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCtt          >  2:752748/1‑150 (MQ=255)
                                                                                                                                                cTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTc      <  1:1547397/150‑1 (MQ=255)
                                                                                                                                                 tGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCa     >  2:265791/1‑150 (MQ=255)
                                                                                                                                                  gACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaa    <  2:932562/150‑1 (MQ=255)
                                                                                                                                                    cGATGCTCAGGGGCAAAAATGTTATCCACATCACAAATTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAac  <  2:1556503/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCAATATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAAC  >  NC_000913/3105384‑3105681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: