Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,194,609 T→A 5.3% intergenic (+84/+114) yqiK → / ← sibD PHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,194,6090TA 5.3% 288.4 / 2.6 117intergenic (+84/+114)yqiK/sibDPHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein
Reads supporting (aligned to +/- strand):  ref base T (52/51);  new base A (4/4);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAG  >  NC_000913/3194461‑3194756
                                                                                                                                                    |                                                                                                                                                   
gcTCACTGGCGGCATTGACTTCACCCCTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt                                                                                                                                                    >  2:1897955/1‑150 (MQ=255)
 cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg                                                                                                                                                   >  1:250072/1‑150 (MQ=255)
     cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTgc                                                                                                                                               <  2:447899/150‑1 (MQ=255)
     cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc                                                                                                                                               <  1:808850/150‑1 (MQ=255)
     cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc                                                                                                                                               <  1:1439600/150‑1 (MQ=255)
     cTGGCGGCATTGACTTCACCATTAACCTCAACAACTCCAGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc                                                                                                                                               <  1:409734/150‑1 (MQ=255)
     cTGGCGGCATTGAATTCACGCTTAACCTCAACAACTCCCGTCGAAGATAAAGCAGAGTTAAACCTCTTCCAGTAATAATCCCTGGCCCTAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc                                                                                                                                               <  1:68956/150‑1 (MQ=37)
          ggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATATTTTATATCTTCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt                                                                                                                                          >  1:818897/1‑150 (MQ=255)
            cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCATGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGGTGCAGCAttt                                                                                                                                        >  1:995995/1‑150 (MQ=255)
            cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAttt                                                                                                                                        >  2:1877415/1‑150 (MQ=255)
             aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGAAGAGTAAAACCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt                                                                                                                                       >  1:1038012/1‑150 (MQ=255)
                gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg                                                                                                                                    <  1:1063334/150‑1 (MQ=255)
                  cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTCCGTg                                                                                                                                  >  1:1008485/1‑150 (MQ=255)
                     cACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTTCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg                                                                                                                               >  2:1582695/1‑150 (MQ=255)
                      aCCCTTAACCTCAAGAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCATAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCATATGATCAGGTTGTTGCAGCATTTTGCGTGTCgg                                                                                                                              <  2:718337/150‑1 (MQ=255)
                       cccTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc                                                                                                                             <  2:1643000/150‑1 (MQ=255)
                           tAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg                                                                                                                         >  1:868194/1‑150 (MQ=255)
                               cTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa                                                                                                                     >  2:480104/1‑150 (MQ=255)
                               cTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa                                                                                                                     >  2:1375467/1‑150 (MQ=255)
                                tCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg                                                                                                                    <  1:388508/150‑1 (MQ=255)
                                tCAACAACTCACGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCGGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg                                                                                                                    >  2:1146224/1‑150 (MQ=255)
                                 taacaaaTCCCGTCTAAGAAAATGCAGAGTAAACCCTATTCCAGGAATAATCCCTGGCCCAAAACGCCGGGGATTTTTTATATCTGCGTTTCGCTATAATGTGCAAATGATTAGGTTGTTTCAGCATTTTGCGTGACGGCTCGGGGAAgg                                                                                                                   <  2:1280276/149‑1 (MQ=21)
                                 caacaaCTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg                                                                                                                   <  2:1832250/150‑1 (MQ=255)
                                       cTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAc                                                                                                             >  1:1892386/1‑150 (MQ=255)
                                         cccGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTg                                                                                                           <  1:1484801/150‑1 (MQ=255)
                                          ccGTCGAAGAAAAAGCAGAGTACACCCTCTTCCAGGAATAATCCCTGGCCCAAACAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGGTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc                                                                                                          <  1:257895/150‑1 (MQ=255)
                                           cGTCGAAGAAATAGGAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCTGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCa                                                                                                         <  1:1746691/150‑1 (MQ=255)
                                           cGTCGAAGAAAAAGCGGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCa                                                                                                         <  2:202304/150‑1 (MQ=255)
                                              cGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCa                                                                                                      >  1:1697350/1‑150 (MQ=255)
                                               gaagaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCag                                                                                                     >  2:452369/1‑150 (MQ=255)
                                                 agaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAATGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg                                                                                                                   <  2:1263511/134‑1 (MQ=14)
                                                 agaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAgg                                                                                                                   >  1:1263511/1‑134 (MQ=17)
                                                 agaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag                                                                                                   >  1:602640/1‑150 (MQ=255)
                                                  gaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga                                                                                                  >  1:1384474/1‑150 (MQ=255)
                                                   aaaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaa                                                                                                 >  1:222650/1‑150 (MQ=255)
                                                    aaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa                                                                                                >  1:1259063/1‑150 (MQ=255)
                                                    aaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGaaa                                                                                                >  1:1352990/1‑150 (MQ=255)
                                                     aaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAg                                                                                               <  2:1183128/150‑1 (MQ=255)
                                                        gCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCAt                                                                                            >  2:1157807/1‑150 (MQ=255)
                                                        gCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCAt                                                                                            >  1:1768300/1‑150 (MQ=255)
                                                          agagTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAACAGCCGGGGATTTTTTATATCTGCGGTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTg                                                                                          <  1:946328/150‑1 (MQ=255)
                                                           gagTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGATGCAGCATTTTGCGTGACGGCTCTGGGAAGGCAAAACTGCATCAGAGAAAGCATTGc                                                                                         >  2:468281/1‑150 (MQ=255)
                                                             gTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGc                                                                                                                                     >  1:990168/1‑104 (MQ=11)
                                                               aaaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg                                                                                     >  2:1550849/1‑150 (MQ=255)
                                                               aaaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg                                                                                     <  1:1897955/150‑1 (MQ=255)
                                                                  cccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGt                                                                                  <  2:250072/150‑1 (MQ=255)
                                                                   ccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc                                                                                 >  1:1847017/1‑150 (MQ=255)
                                                                   ccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc                                                                                 <  1:1582695/150‑1 (MQ=255)
                                                                    ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCt                                                                                >  1:357841/1‑150 (MQ=255)
                                                                      ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTgg                                                                              >  1:528896/1‑150 (MQ=255)
                                                                          cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGAAGTTGCAGCTTTTTGCGTGACCGCTCggg                                                                                                                        >  1:1280854/1‑104 (MQ=11)
                                                                          cAGGAATAATCCCTGGACCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTTCAGCATTTTGCGTGACGGCTCGGGGATTGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGTCGCt                                                                          <  2:1815533/150‑1 (MQ=18)
                                                                           aGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTAGCGCtc                                                                         <  1:799494/150‑1 (MQ=255)
                                                                              aataatCCCTGGCCTAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtcatc                                                                      <  2:331271/150‑1 (MQ=255)
                                                                              aataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTCCGTTCCGCTAAAAAGTGCAAATGATCAGGATGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtcatc                                                                      <  1:961999/150‑1 (MQ=255)
                                                                                 aatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATcaac                                                                   <  2:1309972/150‑1 (MQ=255)
                                                                                  atCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGGTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATcaaca                                                                  <  1:722822/150‑1 (MQ=255)
                                                                                        ggCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCggg                                                            <  1:735566/150‑1 (MQ=255)
                                                                                        ggCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCggg                                                            <  1:1105654/150‑1 (MQ=255)
                                                                                        ggCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCggg                                                            <  1:1877415/150‑1 (MQ=255)
                                                                                          cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAAACGGGGa                                                          >  1:1387862/1‑150 (MQ=255)
                                                                                           ccAACAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGGGCAAATGATCAGGTTGGTGCAGCCTTTTGCGTGACGGCGCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAg                                                         <  1:168910/150‑1 (MQ=18)
                                                                                           ccAAAACGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGGTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAg                                                         <  2:1719921/150‑1 (MQ=255)
                                                                                           ccAAAAAGCCGGTGATTTTTTATATCTGCGTTCCTCTTAAAAGTGCAAATGTTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACATTCGGGGAg                                                         <  1:1843539/150‑1 (MQ=18)
                                                                                           ccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAg                                                         <  1:1127256/150‑1 (MQ=255)
                                                                                                aaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTcagc                                                    <  1:9879/150‑1 (MQ=255)
                                                                                                aaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTcagc                                                    <  1:1375467/150‑1 (MQ=255)
                                                                                                 aGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTcagca                                                   <  2:921457/150‑1 (MQ=255)
                                                                                                   ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGGTAAGGTTGTTGCCGCATTTTTCGTGGCGGCGCGGGGTAGGCAAAACAGCATGAGAGCAAGCATTTGGGGGGGGGTGGCGCTGATGAACAAAGGGGGTGTgaggcag                                                 >  2:810469/1‑146 (MQ=12)
                                                                                                   ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCGCGGGGAAGGCAAAACTGCATCAGAGAGCGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAgg                                                 >  2:371957/1‑150 (MQ=255)
                                                                                                     ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAgggg                                               >  1:701219/1‑150 (MQ=255)
                                                                                                     ggggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGATCATCAACAATCGGGGAGTCAGCAgggg                                               >  2:1734106/1‑150 (MQ=255)
                                                                                                       ggATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGc                                             <  1:1805154/150‑1 (MQ=255)
                                                                                                          ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTCCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGa                                          >  1:1460088/1‑150 (MQ=255)
                                                                                                          ttttttATATCTGCGTGCCGCTAAAAAGTGCAAATGATCAGGTGGTGGCAGCATTTTGCGTGCCGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGa                                          <  1:192200/150‑1 (MQ=255)
                                                                                                              ttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGGTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCTGGGAGTCAGCAGGGGGCTGAAACg                                      <  2:1305455/150‑1 (MQ=255)
                                                                                                               tataTCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAATGCATCAGAGAAAGCATTGCGGGGGGTCGGGCGCTCATCAACAATCGGGGGGACGCAAGGGGCTTCAACggg                                    >  2:1150094/1‑150 (MQ=14)
                                                                                                               tataTCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCGCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACgg                                     <  2:784136/150‑1 (MQ=255)
                                                                                                                    cTGCGTTCGGCTAAAAAGTGAAACTGATCAGGGTGTTGCTGAATTTTGCGGGACGGCGCGGGGAAGGCAAAAATGAATCAGATAAAGCATTGCTTGGTGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGTCTGAAACGGGAAAg                                <  2:300150/150‑1 (MQ=18)
                                                                                                                      gCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGcc                              >  2:504431/1‑150 (MQ=255)
                                                                                                                           ccGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGGGTCAGCAGGGGGCTGAAACGGTAAAGCCCCTcc                         >  2:1152544/1‑150 (MQ=255)
                                                                                                                            cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAACGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTccc                        <  1:1497236/150‑1 (MQ=255)
                                                                                                                            cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTccc                        >  1:1426553/1‑150 (MQ=255)
                                                                                                                              cTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAACACTGCCTCAGAGAAAGCATTGCGAGGGGTCAGGCGCTCATCAACAATCGGGGAGACTCCAGGGGCATGAACGGGGAAGcccccaccgca                     >  1:1804149/1‑143 (MQ=17)
                                                                                                                               tAAAACGGGCAACTGATCCGGGTGGTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGCGAAAGCATTGCGTGGGGGCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCgaa                     <  1:159463/150‑1 (MQ=18)
                                                                                                                               tAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCgaa                     >  1:49085/1‑150 (MQ=255)
                                                                                                                                aaaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCgaag                    >  2:1504348/1‑150 (MQ=255)
                                                                                                                                 aaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCgaaga                   >  1:646336/1‑150 (MQ=255)
                                                                                                                                   aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGaagaag                 <  1:794611/150‑1 (MQ=255)
                                                                                                                                   aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGaagaag                 <  1:1690526/150‑1 (MQ=255)
                                                                                                                                      tGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAACGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAgggg              <  1:452369/150‑1 (MQ=255)
                                                                                                                                        cAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCCTCAGAGAAAGCATTGCGTGGGGTCAGGCGCTCATCAACAATCGGGGTGAAGCAAGGGGCAGAAACGGGAAAGGCCCTGCCGAACAAggggcgg           >  1:129836/1‑147 (MQ=14)
                                                                                                                                        cAAATGATCAGGTTGTTGCAGCATTTTGAGTGACGGCTCGCGGAAGGCAAAAATGCATCCGAGAAAGCATTGCGTTCGGTCTGGCTCTCAACTACAATCGAGGATTCAGCAGGCGGCTGAAACAGCAACGCCCCTGCCGAAGACGGGGGc            >  2:361284/1‑150 (MQ=255)
                                                                                                                                           aTGATCAGGATGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCCCGCATCAACAATCGCGTAGTCAGCAGGGGGCTCAAACCGGACAGCCCCGCCCGAATAAGGGGGccat         >  2:1761987/1‑148 (MQ=255)
                                                                                                                                             gATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTAc       >  2:1233971/1‑150 (MQ=255)
                                                                                                                                              aTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACa      <  2:696321/150‑1 (MQ=255)
                                                                                                                                               tCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCTTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAg     >  1:178131/1‑150 (MQ=255)
                                                                                                                                                cAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGGGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGa    <  1:455815/150‑1 (MQ=255)
                                                                                                                                                  gtttgttgCAGCATTTTGCGTGACGGCTCTGGGAAGTCAAAACTGCATCAGAGAAAGCATTGCGTTGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAATAAGGGGGCAGGACAGaat  >  2:985544/3‑149 (MQ=18)
                                                                                                                                                  ggTTGTTGCAGCATTTTGCGTGACGGCTCGGGTAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGATCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAg  <  2:868194/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAG  >  NC_000913/3194461‑3194756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: