Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,246,564 C→A 5.3% intergenic (+42/‑88) exuT → / → exuR hexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,246,5640CA 5.3% 398.4 / 6.5 133intergenic (+42/‑88)exuT/exuRhexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (57/68);  new base A (5/3);  total (62/71)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

CAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCA  >  NC_000913/3246416‑3246712
                                                                                                                                                    |                                                                                                                                                    
cAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCa                                                                                                                                                     <  1:832953/150‑1 (MQ=255)
cAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCa                                                                                                                                                     <  2:659793/150‑1 (MQ=255)
 aGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGCTCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAg                                                                                                                                                    >  2:710691/1‑150 (MQ=255)
  gtgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACTATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAgg                                                                                                                                                   <  2:159515/150‑1 (MQ=255)
   tgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggc                                                                                                                                                  <  2:863075/150‑1 (MQ=255)
    gtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggcg                                                                                                                                                 >  2:331188/1‑150 (MQ=255)
       cGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCt                                                                                                                                              <  1:1420754/150‑1 (MQ=255)
           cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCttttt                                                                                                                                          <  1:526903/150‑1 (MQ=255)
           cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCttttc                                                                                                                                          <  1:1070840/150‑2 (MQ=255)
            tGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtc                                                                                                                                         <  2:1167908/150‑1 (MQ=255)
             gTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtca                                                                                                                                        >  2:1082910/1‑150 (MQ=255)
               tAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatc                                                                                                                                      <  1:6467/150‑1 (MQ=255)
               tAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttgaaggg                                                                                                                                      <  1:684516/150‑7 (MQ=255)
                aGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatca                                                                                                                                     >  2:213677/1‑150 (MQ=255)
                 ggTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCAc                                                                                                                                    <  2:827254/150‑1 (MQ=255)
                  gTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCTGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACt                                                                                                                                   >  2:1501395/1‑150 (MQ=255)
                   tGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTg                                                                                                                                  <  2:1571311/150‑1 (MQ=255)
                    gcgcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGc                                                                                                                                 <  1:751090/150‑1 (MQ=255)
                       cTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAg                                                                                                                              >  2:6222/1‑150 (MQ=255)
                         ggTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCCCACCTCAACATTAACATTACGTGTAAGTTTTAGTTTCCATTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATAACTGCGAgaa                                                                                                                            >  1:1782588/1‑148 (MQ=255)
                          gTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTag                                                                                                                           >  2:932397/1‑150 (MQ=255)
                           ttATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTTGCGGAGGAAACACATAACGATCCCGAACCGCAACATTAACGTTTGGTGTAAGTTTTATTTTCCATTAAAACCCGCCTTGTAAGTCTGCTTTTTCATCACTGCGATTaga                                                                                                                          >  2:372513/1‑150 (MQ=255)
                              tCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCCTCACTGCGAGTAGAGCt                                                                                                                       >  1:1696498/1‑150 (MQ=255)
                                tGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACCCAGAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTaa                                                                                                                     >  1:936753/1‑150 (MQ=255)
                                tGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTaa                                                                                                                     >  1:391785/1‑150 (MQ=255)
                                 ggACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTaaa                                                                                                                    >  2:1910055/1‑150 (MQ=255)
                                   aCCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACt                                                                                                                  >  1:1089034/1‑150 (MQ=255)
                                      gtgtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACCTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGCCTTTTTCATCACTGCGAGTAGAGATAAACTCga                                                                                                               >  2:1330731/1‑149 (MQ=255)
                                       tgtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCACTTCAAAGCCGCCTTCTCAGGCCGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCa                                                                                                              >  1:15671/1‑150 (MQ=255)
                                        gtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATAACTGCGAGTAGAGCTAAACTCGCaa                                                                                                             >  1:1664418/1‑150 (MQ=255)
                                         ttGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCTCCCCGCAACATTACCGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagt                                                                                                            <  2:1077759/150‑3 (MQ=255)
                                         ttGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagt                                                                                                            <  1:622482/150‑3 (MQ=255)
                                          tGCAGAACAAACCGGCGATTGAGGTGGCGCTGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGGATGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTGTTTCATCACTGGGAGTAGAGCTATAGTCGCaaaa                                                                                                           >  1:1327805/1‑150 (MQ=255)
                                          tGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCaaaa                                                                                                           >  2:797686/1‑150 (MQ=255)
                                          tGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCaaaa                                                                                                           >  2:1845887/1‑150 (MQ=255)
                                           gCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTGTTAGGTTCCCTTCAAAGCCGCCTTCTCAGGCGGGTTTTTCATAACTGCGAGTAGAGCTAAACTAGCAAAAg                                                                                                          >  2:980254/1‑150 (MQ=255)
                                            cAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagtgat                                                                                                         <  1:1902142/150‑6 (MQ=255)
                                             aGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTg                                                                                                        <  1:455024/150‑1 (MQ=255)
                                              gAACAAACCGGCGATTGAGGTGGCGCAGGACACACATAACGATCCCGCACCGCAACATTAAAGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGAGGCTTTTTCATCTCTGCGAGTAGAGCTAAACACGCAAAAGTgg                                                                                                       >  1:48378/1‑150 (MQ=255)
                                              gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTgg                                                                                                       <  2:1531202/150‑1 (MQ=255)
                                              gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTgg                                                                                                       >  1:1636299/1‑150 (MQ=255)
                                                aCAAACCGGCGATTGAGGTGGAGCAGGAAACAGATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGta                                                                                                     <  1:276443/150‑1 (MQ=255)
                                                 cAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCATTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGATAAACTCGCAAAAGTGGtat                                                                                                    >  2:205470/1‑150 (MQ=255)
                                                  aaaCCGGCGATTGAGGTGTCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGtata                                                                                                   <  2:1648761/150‑1 (MQ=255)
                                                  aaaCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGtata                                                                                                   <  1:557903/150‑1 (MQ=255)
                                                  aaaCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGtata                                                                                                   <  2:244616/150‑1 (MQ=255)
                                                   aaCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATaa                                                                                                  >  1:600861/1‑150 (MQ=255)
                                                    aCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAAc                                                                                                 >  1:611132/1‑150 (MQ=255)
                                                          gATTGAGGTGGCGCAGGACACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTAAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAatat                                                                                           <  1:297291/150‑1 (MQ=255)
                                                          gATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAatat                                                                                           >  2:156845/1‑150 (MQ=255)
                                                          gATTGAGGGGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAatat                                                                                           <  1:1407556/150‑1 (MQ=255)
                                                              gAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCATCATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTTCGAGTAGAGCTAAACTCGCAAATGTGGTAGACCAAATATAGTc                                                                                       >  1:329503/1‑150 (MQ=255)
                                                                   ggCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCg                                                                                  <  2:729487/150‑1 (MQ=255)
                                                                     cgcAGGAAACACATAACGATCCCGCACCGCCCCATTAACGTTTCGTGGAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTGTCATCCCTGCGAGTAGAGCTAAACTAGCAAAACTGGGATTACAATTATAGTCTGCCGTa                                                                                >  2:1032645/1‑150 (MQ=255)
                                                                       catgAAACACATAAGGATGCCTCACCGCAACATTAAAGTTTCGTTTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCTGCTTTTTCATCACTTAGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAATTATAGTCTGCCGTATc                                                                              <  1:275223/147‑1 (MQ=255)
                                                                       cAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATc                                                                              >  1:1144658/1‑150 (MQ=255)
                                                                        aGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCa                                                                             >  2:1620214/1‑150 (MQ=255)
                                                                              cacaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTg                                                                       >  2:434840/1‑150 (MQ=255)
                                                                              cacaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGACGTATCATGCCTg                                                                       >  2:1823783/1‑150 (MQ=255)
                                                                               acaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCAGCCGTCGCAGGCGGATTTTTCATAACTGCGAGTAGAGCTAAACACGCAAAAGTGGTATAACAAATATAGTCTTCCGTTACATGCCTgg                                                                      >  1:1150796/1‑150 (MQ=255)
                                                                                caTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACTAATATAGTCTGCCGTATCATGCCTg                                                                       >  2:578814/1‑148 (MQ=255)
                                                                                caTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTg                                                                       <  1:578814/148‑1 (MQ=255)
                                                                                caTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                     <  1:1309943/150‑1 (MQ=255)
                                                                                  tAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAgc                                                                   <  1:115115/150‑1 (MQ=255)
                                                                                  tAACGATCACGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTACAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAACCTCGCAAAAGTGGTATAACAAATATAGTCTGCCGAATCATGACTGGAgc                                                                   >  1:520087/1‑150 (MQ=255)
                                                                                     cGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAATAGTGGGATAACAATTATTGTCTGCCGTATCATGCCTGGCGAGCa                                                                >  2:188062/1‑150 (MQ=255)
                                                                                     cGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCat                                                               <  1:1853700/150‑1 (MQ=255)
                                                                                      gATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCat                                                               <  2:67088/150‑1 (MQ=255)
                                                                                      gATACCGCACCGCAACATTTACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCat                                                               <  1:1240526/150‑1 (MQ=255)
                                                                                       aTCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTACAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                              <  1:676733/150‑1 (MQ=255)
                                                                                       aTCCCGCAACGCAACATTAACGTTTCGTGTAAGTATTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                              <  1:1746025/150‑1 (MQ=255)
                                                                                               ccgcaACATTAACTTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATc                                                      >  2:19453/1‑150 (MQ=255)
                                                                                               ccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATc                                                      <  1:1002707/150‑1 (MQ=255)
                                                                                                  caACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGTAAATCACt                                                   >  2:1491667/1‑150 (MQ=255)
                                                                                                  caACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACt                                                   <  1:806556/150‑1 (MQ=255)
                                                                                                      aTTAACGTTACGTGTAAGATTTAGTTTCACTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGGAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGTAGCGCATATGGATATCACTGAAc                                               >  2:404244/1‑150 (MQ=255)
                                                                                                            gTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGcc                                         <  1:331188/150‑1 (MQ=255)
                                                                                                               tCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGATTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGtt                                      <  2:1434771/150‑1 (MQ=255)
                                                                                                                 gtgtAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGATATCACTGAACCACGCCGTTTg                                    <  1:265003/150‑1 (MQ=255)
                                                                                                                 gtgtAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTg                                    <  2:1099530/150‑1 (MQ=255)
                                                                                                                    tAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATAATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTAt                                 >  2:986658/1‑150 (MQ=255)
                                                                                                                         tttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGTTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAATTCACTGAACCACGCCGTTTGTATcaaca                            >  1:367553/1‑150 (MQ=255)
                                                                                                                         tttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaaca                            <  1:1578680/150‑1 (MQ=255)
                                                                                                                         tttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaaca                            <  1:1648019/150‑1 (MQ=255)
                                                                                                                             gTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACtt                        >  2:1477673/1‑150 (MQ=255)
                                                                                                                               ttCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGc                      <  1:156845/150‑1 (MQ=255)
                                                                                                                                tCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGcc                     <  1:532339/150‑1 (MQ=255)
                                                                                                                                  ccTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGc                   >  1:427855/1‑150 (MQ=255)
                                                                                                                                   tgaaaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCt                  >  2:835682/5‑150 (MQ=255)
                                                                                                                                    ttCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTg                 <  2:1577963/150‑1 (MQ=255)
                                                                                                                                    gaaaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTg                 >  1:1197223/4‑150 (MQ=255)
                                                                                                                                      cAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAc               >  1:1318764/1‑150 (MQ=255)
                                                                                                                                      aaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGACGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAc               >  1:975273/2‑150 (MQ=255)
                                                                                                                                       aaaGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAcc              >  2:4533/1‑150 (MQ=255)
                                                                                                                                       aaaGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCAGGAGCGCATATGGAAATCACTGAACCACGCCGGTTGTATCAACAACTTGCCGCGGAcc              >  1:1177247/1‑150 (MQ=255)
                                                                                                                                        aaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCt             >  1:1899607/1‑150 (MQ=255)
                                                                                                                                         aGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTg            <  1:1848841/150‑1 (MQ=255)
                                                                                                                                              ccTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAaga       <  1:932397/150‑1 (MQ=255)
                                                                                                                                                 tctgAGGCTGCTTTTTGATCACTGCGAGTAGAGCTAAAGTCGCAAAAGTGGTATAACAAATATAGTGTGCCGTATCATGCCTGGAGCTCATATGGATATCACTGAACCACGCCGTTTGTATCTACAACTTGCCGCTGACCTGAAAGAgcg    <  2:1782588/150‑1 (MQ=255)
                                                                                                                                                   tcAGGCGGATTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTTTCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCa  <  1:213677/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCA  >  NC_000913/3246416‑3246712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: