Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,109 A→T 5.8% K766* (AAA→TAA) ‡ yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1090AT 5.8% 336.8 / 5.9 117K766* (AAA→TAA) ‡yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (53/57);  new base T (3/4);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 120 total aligned reads displayed.

CTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGT  >  NC_000913/3290961‑3291258
                                                                                                                                                    |                                                                                                                                                     
ctcGGTAGCTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACCACACAGTTTGTGGTGCCAAACAGAGGGGCAATGGTAGCTGCGAGTTTCAACGCCAATATCGGTTATCGCGGACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                                      >  1:1731554/1‑150 (MQ=255)
ctcGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAAAAAACAAAACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                                      >  1:948708/1‑150 (MQ=255)
  cGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAAc                                                                                                                                                    <  1:646133/150‑1 (MQ=255)
     tAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGt                                                                                                                                                 >  2:105236/1‑150 (MQ=255)
        aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCAAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCACCGGTAAACCGTTGc                                                                                                                                              >  1:1688024/1‑150 (MQ=255)
         tactacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGcc                                                                                                                                             >  2:1562982/1‑150 (MQ=255)
          actaaGCAGCTGCCCGATAACGTCTATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTTCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGGGATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGccc                                                                                                                                            <  2:1312901/150‑1 (MQ=255)
           ctacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgat                                                                                                                                           <  1:990183/150‑4 (MQ=255)
           ctacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgat                                                                                                                                           <  1:1353003/150‑4 (MQ=255)
           ctacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCt                                                                                                                                           >  2:437522/1‑150 (MQ=255)
             acGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCttt                                                                                                                                         >  2:1258651/1‑150 (MQ=255)
              cGCAGCTGCCCGATCACGGCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcgc                                                                                                                                        <  1:920175/150‑7 (MQ=255)
              cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcgc                                                                                                                                        <  1:1747965/150‑7 (MQ=255)
              cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                        >  2:1654326/1‑150 (MQ=255)
              cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATAGCGGTTATCGCGTACGTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                        >  2:1388057/1‑150 (MQ=255)
               gCAGCCGCCCGATAACCTCCCTCTTGAACAAACAACACAGTCTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAAAATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTgg                                                                                                                                       <  2:1217100/150‑1 (MQ=255)
                ccgCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                                                                      <  1:1114246/148‑1 (MQ=255)
                  gctgctCTATAACGTCGATCTTGATCAAACAACACAGTTTGTGGTGCCTAACAGAGGTTCTATTGTAGCGGCGCGGTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCATCGATCGCAACGGTAAACCGTTGACCTTTGgcgc                                                                                                                                    <  1:903817/150‑1 (MQ=255)
                   cTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGGAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTGGGCGct                                                                                                                                   >  2:1051441/1‑150 (MQ=255)
                    tGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGctc                                                                                                                                  <  2:1586866/150‑1 (MQ=255)
                      cccGATAACGTCGATCTTGAACAAACAGCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGGTACAGTCAGCGATCGCAACGGTAGGCCGTTGCCCTTTGgcgcgcgg                                                                                                                                >  2:1265043/1‑146 (MQ=255)
                        cGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGCTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                                                                              >  1:32673/1‑150 (MQ=255)
                        cGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACACAGGTGCAATTGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                                                                              <  1:1099640/150‑1 (MQ=255)
                           tAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCAAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAg                                                                                                                           >  1:1148970/1‑150 (MQ=255)
                            aaCGTCGATCATGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGc                                                                                                                          <  1:903547/150‑1 (MQ=255)
                             aCGTCGATCTAGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCa                                                                                                                         <  1:127095/150‑1 (MQ=255)
                               gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                                                                       <  1:1514622/150‑1 (MQ=255)
                               gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                                                                       <  1:748879/150‑1 (MQ=255)
                                   ctCTTGAACAAACAACCCAGCTTGGCGCGCCCAACAGAGGCGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGGCCGCGATCGCAACGGTAAACCGTTGCCCTATGGCGCTCTTGCCAGCAACgatg                                                                                                                   <  2:105681/149‑1 (MQ=255)
                                   aTCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                                                                   <  1:846449/150‑1 (MQ=255)
                                   aTCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                                                                   <  2:955918/150‑1 (MQ=255)
                                   aTCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTGTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                                                                   <  1:1182486/150‑1 (MQ=255)
                                   aTCTTGAACAAACAACACAGTGTGTGGTGCCCCACAGAGGTGCAATGGTAGCGGCGCGTTGCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCGTGCCAGCAACgatg                                                                                                                   >  2:1194396/1‑150 (MQ=255)
                                    tCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatga                                                                                                                  <  1:1648072/150‑1 (MQ=255)
                                      ttGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                                                                                >  2:1608423/1‑150 (MQ=255)
                                       tGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATAc                                                                                                               <  2:1598710/150‑1 (MQ=255)
                                       tGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGACAGCGATCGCAACGGTAAACCTTTGCCCTTAGGCGATCTTGCCAGCAACGAGGATAc                                                                                                               >  2:1113806/1‑150 (MQ=255)
                                        gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCATTGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACg                                                                                                              >  2:787466/1‑150 (MQ=255)
                                         aacaaacaACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                                                                             <  1:682773/150‑1 (MQ=255)
                                         aacaaacaACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                                                                             >  1:1467068/1‑150 (MQ=255)
                                           caaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                           <  1:1004622/150‑1 (MQ=255)
                                           caaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                           >  2:551054/1‑150 (MQ=255)
                                           ataacaacACAGTTTGTGGTGCCCAACAGAGGGGAATTGGTTGCGGCGCGTGTCAACGCAAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                           <  1:1858687/148‑1 (MQ=255)
                                            aaacaaCACAGTTTGTGGTGCCGACCAGAGGTGCAATGGTAGCGGCGCGTGTCACGGGCAATATCGGTTTTCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAGCCGTTGCCGGTTGGCGCGGTTGGCGGGAAGGATGGTGCggggg                                                                                                          >  2:674609/1‑149 (MQ=255)
                                             aacaacACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGca                                                                                                         >  1:676913/1‑150 (MQ=255)
                                             aacaacACAGTTTGCGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACTGGGca                                                                                                         <  2:1433262/150‑1 (MQ=255)
                                                   accgTTTGGGGCGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCATTATCGGTTTTCGAGAACTTGTTACAGTCAGCGATCGCAACGTTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                                                                   <  2:284968/147‑1 (MQ=255)
                                                   acaGTTTGTGGTGCCCAACAGCGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                                                                   <  2:1833818/150‑1 (MQ=255)
                                                      gTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAt                                                                                                <  2:1662069/150‑1 (MQ=255)
                                                      gTTTGTGGGGCCCAACAGAGGGGCTATGGGAGCGGCGCGTTTCAACGCCAATAGCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAt                                                                                                <  2:1423287/150‑1 (MQ=255)
                                                       tttGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtc                                                                                               <  1:905962/150‑1 (MQ=255)
                                                          gtggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCTATCTCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtc                                                                                            <  2:675834/150‑1 (MQ=255)
                                                          gtggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtc                                                                                            <  1:103213/150‑1 (MQ=255)
                                                           tggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtcg                                                                                           <  1:437522/150‑1 (MQ=255)
                                                           tggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtcg                                                                                           >  1:579399/1‑150 (MQ=255)
                                                             gtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAt                                                                                         <  1:68267/150‑1 (MQ=255)
                                                                  cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCCGCAACGATGATGCGGGGCAACAAAGTTTGGTCGATGAggg                                                                                    >  2:1580561/1‑150 (MQ=255)
                                                                  cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGAGGATACGGGGCAACAAAGTATCGTCGATGAggg                                                                                    >  2:1049669/1‑150 (MQ=255)
                                                                    aCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                                                                                  <  1:1543898/150‑1 (MQ=255)
                                                                          gTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATAc                                                                            >  2:1495820/1‑150 (MQ=255)
                                                                            gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                          >  2:1528314/1‑150 (MQ=255)
                                                                             cAATGGTAGCGGCGCGTTTAAACGCCAATATCGGATATCGCGTACTTGTTACAGTCTGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                                                                         <  2:1520799/150‑1 (MQ=255)
                                                                                      cggcgCGTTTCAACGACAATATCGGTTATCGCTTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTg                                                                <  2:588509/150‑1 (MQ=255)
                                                                                       ggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                               >  2:678576/1‑150 (MQ=255)
                                                                                        gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTggg                                                              <  2:1574691/150‑1 (MQ=255)
                                                                                            gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCATTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGatat                                                          >  1:711597/1‑150 (MQ=255)
                                                                                            gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGACCTTTGGAGCTCTTTCCAGAAACGATGCTACGGGGCAACAAAGTATCGTAGAGGAGGGCGGCAGACGAGATCTCTCTGGGagat                                                          >  2:1253704/1‑150 (MQ=255)
                                                                                             tttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATc                                                         >  2:1484021/1‑150 (MQ=255)
                                                                                             tttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATc                                                         <  2:1467068/150‑1 (MQ=255)
                                                                                              ttCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTGTCTGGGAGATCg                                                        >  2:317225/1‑150 (MQ=255)
                                                                                                 aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGCTGATACGGGGCCACAAAGGATCGTCGATGAGGGCGGCATACGATATCTCTCTGGGATATCGAGt                                                     >  1:1412567/1‑150 (MQ=255)
                                                                                                 aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCGTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAGGAGGGCGGCATACGATATCTCTCTGGGATATCGAGt                                                     >  2:48972/1‑150 (MQ=255)
                                                                                                   cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                   >  1:1021339/1‑150 (MQ=255)
                                                                                                     ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGACAGCACCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAAt                                                 >  1:1152057/1‑150 (MQ=255)
                                                                                                              gTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCt                                        >  1:1525164/1‑150 (MQ=255)
                                                                                                               ctctCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTg                                       <  1:674609/147‑1 (MQ=255)
                                                                                                                    gcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTg                                  <  2:428771/150‑1 (MQ=255)
                                                                                                                       tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTAc                               >  2:1334317/1‑150 (MQ=255)
                                                                                                                        aCTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACg                              >  2:1476165/1‑150 (MQ=255)
                                                                                                                            gTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTgg                          >  1:1358536/1‑150 (MQ=255)
                                                                                                                               aCAGGCAGCGATCGCAACGGTAAACCGGTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa                       <  1:523197/150‑1 (MQ=255)
                                                                                                                                cAGTCAGCGATCGCAACGGTAAGCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTCGGGaa                      >  1:73499/1‑150 (MQ=255)
                                                                                                                                cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGaa                      >  2:1248990/1‑150 (MQ=255)
                                                                                                                                cAGTCAGCGAACGCAACGGCAAACCGTTGCCCTTTGGCGCTCTTGCCACCAACGATGAAACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTAAATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGaa                      >  1:1903907/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCATCAAAGTCTCGTGGATGAGGGCGGCATACTATATCTCTCTGGGATTTCGTGTAACTCACAAAGCTGGAGTGTCGGGTGGGGaatg                    >  1:1455017/1‑148 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                    >  2:1848440/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                    >  1:1816256/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCGGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                    >  1:804067/1‑150 (MQ=255)
                                                                                                                                   ccAGCGAACGCAACGGCAAACCGGTGCCCTTTGGCGCTCTTGCCCGCAACGAAGCAACGTGGCAACAAAGTATAGTCGAAGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATc                   <  1:1051441/149‑1 (MQ=255)
                                                                                                                                    cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa                  >  1:1019338/1‑150 (MQ=255)
                                                                                                                                      gCGATCGCCACTGTAAACCGATGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg                <  2:1419604/150‑1 (MQ=255)
                                                                                                                                        gATCGCAACGGTAAACCGGTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCa              <  2:1763445/150‑1 (MQ=255)
                                                                                                                                         aTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACATAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAg             <  2:482728/150‑1 (MQ=255)
                                                                                                                                          tCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGa            <  1:1654326/150‑1 (MQ=255)
                                                                                                                                          gggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGa            >  2:1613116/3‑150 (MQ=255)
                                                                                                                                           ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt           >  1:1904485/2‑150 (MQ=255)
                                                                                                                                           ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt           >  1:194427/2‑150 (MQ=255)
                                                                                                                                           cGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt           <  1:1265043/150‑1 (MQ=255)
                                                                                                                                                 ggTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaacaa     <  1:551054/150‑1 (MQ=255)
                                                                                                                                                    aaaCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTCTACGCTGGGGAAATCAGGCAGATCAACAATGt  >  2:1526689/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGT  >  NC_000913/3290961‑3291258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: