Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,300,232 A→T 5.4% L89F (TTA→TTT yhbQ → GIY‑YIG nuclease superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,300,2320AT 5.4% 343.9 / 4.5 117L89F (TTA→TTTyhbQGIY‑YIG nuclease superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (63/47);  new base T (4/3);  total (67/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTGATGC  >  NC_000913/3300083‑3300381
                                                                                                                                                      |                                                                                                                                                      
cTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTa                                                                                                                                                        <  2:1401856/150‑1 (MQ=255)
  gcgcGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAg                                                                                                                                                      <  2:1316580/150‑1 (MQ=255)
   cgcgGGAAAGCAGAACTACCGCTGGTGGTAGCCGCGCCAGCCGGCGTGCGTTCGCTGGCCCTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGc                                                                                                                                                     <  2:1192821/150‑1 (MQ=255)
     cgGGAAACGAGAACTAACGCGGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAg                                                                                                                                                   <  2:681850/150‑1 (MQ=255)
           aGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCCGGGTTTGCAGAGCTGTTAAGCAGTCTGCa                                                                                                                                            >  2:178104/1‑150 (MQ=255)
           aGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCa                                                                                                                                            <  1:1770287/150‑1 (MQ=255)
              agaACTCACGCTGGGGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGCGCGTCTGGTAGCGGAAGGCGCAGGGCTTGCAGACTGCTTAACAGCTCTGCAAAc                                                                                                                                         <  1:1573371/150‑1 (MQ=255)
              agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGACTGCTTAACAGCTCTGCAAAc                                                                                                                                         <  1:1887805/150‑1 (MQ=255)
              agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTACAGAGCTGTTAAGCAGTCTGCAAAc                                                                                                                                         <  2:512061/150‑1 (MQ=255)
               gaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCCGGGTTTGCACAGCTGATAAGCAGACTGCAAAcc                                                                                                                                        >  2:852858/1‑150 (MQ=255)
                 aCTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAAcccc                                                                                                                                      <  2:784858/150‑1 (MQ=255)
                  cTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCg                                                                                                                                     >  2:1289914/1‑150 (MQ=255)
                  cTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCg                                                                                                                                     >  1:1646735/1‑150 (MQ=255)
                   tAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAACCGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCgg                                                                                                                                    <  1:1195126/150‑1 (MQ=255)
                     aCGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGgag                                                                                                                                  <  1:372550/150‑1 (MQ=255)
                       gcgggggtTTTCCGCGCCAGTGGGCGAGCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCCAACGGAAAAACGCGCGGCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGAt                                                                                                                                <  2:471662/144‑1 (MQ=255)
                       gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGGTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGCGCTGTTAAGCAGTCTGCAAACCCCGGAGAt                                                                                                                                >  2:128345/1‑150 (MQ=255)
                        cTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGAtt                                                                                                                               >  1:1879120/1‑150 (MQ=255)
                          ggTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTaa                                                                                                                             >  2:1139186/1‑150 (MQ=255)
                            tgtTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGTGATTaaaa                                                                                                                           >  1:85472/1‑150 (MQ=255)
                              ttttCCTCGCCAGTGGGCGATCGATCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTACCGCAAGGCGCCGGGTTTGCAGAGCTGTTAAGCAGTCTGCATACCCCGGAGATTAAAAGc                                                                                                                         >  2:1851763/1‑150 (MQ=255)
                               tttCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAGCCCCGGAGATTAAAAGCg                                                                                                                        >  1:496402/1‑150 (MQ=255)
                                 tCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAACGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGAt                                                                                                                      <  1:1424782/150‑1 (MQ=255)
                                  ccGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGAtt                                                                                                                     <  1:576895/150‑1 (MQ=255)
                                    gcgcCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGTGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGTCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGa                                                                                                                   <  1:304628/150‑1 (MQ=255)
                                    gcgcCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGGCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGa                                                                                                                   <  2:1549795/150‑1 (MQ=255)
                                     cgcCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGaa                                                                                                                  <  1:541022/150‑1 (MQ=255)
                                      gcCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGaaa                                                                                                                 <  1:1020309/150‑1 (MQ=255)
                                       ccAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAAt                                                                                                                >  1:776070/1‑150 (MQ=255)
                                          gTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGcg                                                                                                             >  1:446420/1‑149 (MQ=255)
                                          gTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCt                                                                                                             >  2:1528574/1‑150 (MQ=255)
                                            gggCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCg                                                                                                           >  2:628628/1‑150 (MQ=255)
                                              gCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTg                                                                                                         <  2:544684/150‑1 (MQ=255)
                                              gCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCGGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTg                                                                                                         <  2:549696/150‑1 (MQ=255)
                                                   cGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACt                                                                                                    >  1:395561/1‑150 (MQ=255)
                                                       cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAAc                                                                                                >  1:571975/1‑150 (MQ=255)
                                                        gCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAAcc                                                                                               >  1:944441/1‑150 (MQ=255)
                                                           ggCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAgg                                                                                            <  2:1082335/150‑1 (MQ=255)
                                                             ggTTACGGGCGGAATTTCGCGTTAAGCAACTGACGAAACTGTAAATAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGcc                                                                                          <  2:1884240/149‑1 (MQ=255)
                                                              gTTACGGGCGGAATATCGCTGTAAGCAACTGACCAAACGGCAAACAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCg                                                                                         <  2:411172/150‑1 (MQ=255)
                                                              gTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCg                                                                                         >  1:871301/1‑150 (MQ=255)
                                                                    ggCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACg                                                                                   <  2:166168/150‑1 (MQ=255)
                                                                    ggCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACg                                                                                   >  2:1322035/1‑150 (MQ=255)
                                                                      cGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCACCCAGGCCGGTAACGcc                                                                                 >  1:181191/1‑150 (MQ=255)
                                                                          atatCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTc                                                                             <  1:726946/150‑1 (MQ=255)
                                                                            atCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGTGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAg                                                                           >  1:738661/1‑150 (MQ=255)
                                                                            atCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAg                                                                           >  2:19687/1‑150 (MQ=255)
                                                                            atCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAg                                                                           >  1:579250/1‑150 (MQ=255)
                                                                             tCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAgc                                                                          >  2:1512802/1‑150 (MQ=255)
                                                                             tCGCGATAAGCAACTGACCAAACGGCAAAAAGAGCTTCAGGTATCCGAAGGCGCACCGATTCAAGATCTGTTAAGCAGTCTGCAAACCCACGAGAATAAAAGCGATTGAAATGCTCCATATACTCAACCAGGCCCGTAACGCCATTCAgc                                                                          >  2:1870150/1‑150 (MQ=255)
                                                                              cgcgTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGTTACTCAACCAGGCCGGTAACGCCATTCAgcg                                                                         >  1:392399/1‑150 (MQ=255)
                                                                              cgcgTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTCGTAGCGGAAGCCGCCGGGTTTGAAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAACAGCGATAGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAgcg                                                                         >  2:735546/1‑150 (MQ=255)
                                                                               gcgTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTAAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCACCCAGGCCGGTAACGCCATTCAgcgc                                                                        >  1:255703/1‑150 (MQ=255)
                                                                                cgTTAAGCAACTGACCAAACGGCAAAAAGACCGTCTGGTAGCGGAATGCGCATGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGcgcg                                                                       <  1:1031574/150‑1 (MQ=255)
                                                                                 gTTAAGCAACTGACCAACCGGCAAACCGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGt                                                                      <  2:355559/150‑1 (MQ=255)
                                                                                 gTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGt                                                                      <  2:607291/150‑1 (MQ=255)
                                                                                   tAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCa                                                                    >  1:1723979/1‑150 (MQ=255)
                                                                                       cAACTGCCCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCCGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCg                                                                >  2:1374966/1‑150 (MQ=255)
                                                                                        aaCTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGc                                                               >  2:1755533/1‑150 (MQ=255)
                                                                                         aCTGACCAAACGGCAAAAAGATCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCt                                                              <  2:1157064/150‑1 (MQ=255)
                                                                                          cTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTa                                                             >  1:944246/1‑150 (MQ=255)
                                                                                            gACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTaaa                                                           >  2:1904454/1‑150 (MQ=255)
                                                                                             aCCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCGCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAAc                                                          <  2:178866/150‑1 (MQ=255)
                                                                                              ccAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACg                                                         >  1:1366310/1‑150 (MQ=255)
                                                                                                 aaCGGCAAATAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATg                                                      <  1:147004/150‑1 (MQ=255)
                                                                                                    ggCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAACAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTAc                                                   >  2:152017/1‑150 (MQ=255)
                                                                                                     gCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTAcc                                                  >  1:1268279/1‑150 (MQ=255)
                                                                                                     gCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTAcc                                                  >  2:1606630/1‑150 (MQ=255)
                                                                                                     gCAAAAAGAGCGGCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTAcc                                                  <  1:332692/150‑1 (MQ=255)
                                                                                                         aaaGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGa                                              >  2:715545/1‑150 (MQ=255)
                                                                                                         aaaGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGa                                              >  1:1043973/1‑150 (MQ=255)
                                                                                                           agagCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCAGGAAg                                            >  1:1817058/1‑150 (MQ=255)
                                                                                                              gCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGgcg                                         >  1:137731/1‑150 (MQ=255)
                                                                                                                  cTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCttt                                     <  2:1017254/150‑1 (MQ=255)
                                                                                                                   tgggaccGGAAGGCGCAGGTTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAACAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTc                                    <  1:1761619/144‑1 (MQ=255)
                                                                                                                   tGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTTAGCTGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTc                                    <  2:1796807/150‑1 (MQ=255)
                                                                                                                   tGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCCTCCGCTAAAGGATGTACCTGGAAGGCGCTTTc                                    >  2:1023089/1‑150 (MQ=255)
                                                                                                                   tGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTc                                    <  2:1728055/150‑1 (MQ=255)
                                                                                                                   tGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTc                                    <  2:1293161/150‑1 (MQ=255)
                                                                                                                      tAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATCCTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTCGAAGGCGCTATCAGt                                 >  1:323809/1‑150 (MQ=255)
                                                                                                                        gCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGc                               >  1:605303/1‑150 (MQ=255)
                                                                                                                          ggAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGccc                             <  2:1646735/150‑1 (MQ=255)
                                                                                                                          ggAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGAGTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTCACGCCATTCCGCGCGTCATCCGCGAAACGATGTACCTGGAAGGCGCTTTCAGTGccc                             >  1:1365441/1‑150 (MQ=255)
                                                                                                                              ggCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCATTCAGTGCCCGGcc                         >  2:149996/1‑150 (MQ=255)
                                                                                                                                cgcAGGGTTTGCAGAGCTGTTTAGCAGACTGCAAACCGCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGGCCTTCAGCGCGTCCTCCCCTAAACGATGTACCTGGAAGGCGCTTTCAGTGACCGGACac                       >  2:284659/1‑149 (MQ=255)
                                                                                                                                cgcAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAg                       >  2:1527675/1‑150 (MQ=255)
                                                                                                                                 gcAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGc                      >  1:296816/1‑150 (MQ=255)
                                                                                                                                 gcAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGc                      >  1:1545872/1‑150 (MQ=255)
                                                                                                                                   ggggTTTGCAGACTGCTTAACAGCTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCgg                    >  1:231728/2‑150 (MQ=255)
                                                                                                                                   ggggTTTGCAGACTGCTTAACAGCTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCgg                    >  2:536725/2‑150 (MQ=255)
                                                                                                                                   ggggTTTGCAGACTGCTTAACAGCTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCgg                    >  2:529363/2‑150 (MQ=255)
                                                                                                                                   aGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCgg                    <  1:1528574/150‑1 (MQ=255)
                                                                                                                                    gggTTTGCAGACTGCTTAACCGCTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCCGCGGc                   >  1:775906/1‑150 (MQ=255)
                                                                                                                                    gggTGTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAACTGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGc                   <  1:928132/150‑1 (MQ=255)
                                                                                                                                     ggTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCAACCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCa                  >  2:811073/1‑150 (MQ=255)
                                                                                                                                          gCAGAGCTGTTAAGCAGGCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCa             <  1:1442398/150‑1 (MQ=255)
                                                                                                                                           cAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAg            <  1:1527675/150‑1 (MQ=255)
                                                                                                                                           cAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAg            >  1:525197/1‑150 (MQ=255)
                                                                                                                                              agCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATc         <  1:178104/150‑1 (MQ=255)
                                                                                                                                                      aaGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGGCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTGATgc  <  2:1386130/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTGATGC  >  NC_000913/3300083‑3300381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: