Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,377,770 A→C 5.6% intergenic (‑350/+45) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,7700AC 5.6% 318.4 / 5.2 114intergenic (‑350/+45)sspA/rpsIstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base A (65/41);  new base C (3/4);  total (69/45)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

AAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTC  >  NC_000913/3377624‑3377919
                                                                                                                                                  |                                                                                                                                                     
aaaaGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGc                                                                                                                                                    >  2:641087/1‑150 (MQ=255)
          cgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTc                                                                                                                                          >  2:24622/1‑150 (MQ=255)
          cgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTc                                                                                                                                          >  1:1469217/1‑150 (MQ=255)
          cgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGGTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGttttttt                                                                                                                                          <  2:647322/150‑2 (MQ=255)
          cgcTTTAGTCTGAAATAGGTGACTTAGTCCCTTATTGGCTATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCATTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttttta                                                                                                                                          <  1:1000434/150‑2 (MQ=255)
            cTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGtttttttat                                                                                                                                        <  2:202122/150‑4 (MQ=255)
             tttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGaa                                                                                                                                       <  1:798452/150‑1 (MQ=255)
              ttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTATTTCGaaa                                                                                                                                      >  2:1456478/1‑150 (MQ=255)
               tAGTCTGAAATAGTTGACTTAGTCCCATATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGtttttttatagc                                                                                                                                     <  2:693069/150‑7 (MQ=255)
                 gTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAAtt                                                                                                                                   >  1:840250/1‑150 (MQ=255)
                    tGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTGTTCGAAAATTGtt                                                                                                                                >  2:351911/1‑150 (MQ=255)
                     gAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGATTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGGGGGTTTTTTCGAAAAtttttt                                                                                                                               >  2:275783/1‑150 (MQ=255)
                      aaaTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt                                                                                                                              >  2:305886/1‑150 (MQ=255)
                      aaaTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt                                                                                                                              >  2:1439272/1‑150 (MQ=255)
                       aaTAGTTGACTTAGTCCCTTCTTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTc                                                                                                                             <  1:346768/150‑1 (MQ=255)
                         tAGTTGACTTCGTCCCTTATTGGCGATGGGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCGGATTCAGATTTTAGCTATAAAAAAACCCGCCGACGCGGGTTTTTTCGAAAATTGTTTTCTg                                                                                                                           <  2:1501698/150‑1 (MQ=255)
                         tAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTg                                                                                                                           >  1:1326848/1‑150 (MQ=255)
                           gTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGcc                                                                                                                         >  2:848909/1‑150 (MQ=255)
                             tGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCgg                                                                                                                       <  2:385175/150‑1 (MQ=255)
                             ggACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCgg                                                                                                                       <  1:1584074/149‑1 (MQ=255)
                                cTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGc                                                                                                                    <  2:1479843/150‑1 (MQ=255)
                                    gTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGaa                                                                                                                >  1:153026/1‑150 (MQ=255)
                                      cccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                              <  1:174145/150‑1 (MQ=255)
                                      cccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                              >  1:1071015/1‑150 (MQ=255)
                                      cccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                              >  1:1490958/1‑150 (MQ=255)
                                      cccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGCCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGTTTTTACCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTTTTTTCTGCCGGACAAGAAGc                                                                                                              >  2:757933/1‑150 (MQ=255)
                                           aTTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAAtt                                                                                                         >  1:699003/1‑150 (MQ=255)
                                              ggCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCATATTTTAGCTATAAAAAAAAACGCCGAAGCGGGTTTTTTCGAAAATTGTTATATGCCGGAGAAGAAGCCAATAAAc                                                                                                      >  1:832764/1‑150 (MQ=255)
                                              ggCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCTAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGTAGCAGAAGCCAATTAAc                                                                                                      >  2:143398/1‑150 (MQ=255)
                                              ggCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAAc                                                                                                      >  1:1716363/1‑150 (MQ=255)
                                               gCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg                                                                                                     <  1:1513426/150‑1 (MQ=255)
                                               gCGATGTGGTATTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTGTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGCAAATTAACg                                                                                                     >  1:984790/1‑150 (MQ=255)
                                                cGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGt                                                                                                    >  2:824390/1‑150 (MQ=255)
                                                  aTGTGGTTTTTGTATTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGttt                                                                                                  <  1:777630/150‑1 (MQ=255)
                                                   tgtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCTGATTTTAGCTATAAAAAAACCCGCCGCAGCGGGTTTTTTCGAATATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg                                                                                                 <  1:984565/150‑1 (MQ=255)
                                                   tgtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg                                                                                                 >  2:1142238/1‑150 (MQ=255)
                                                   tgtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg                                                                                                 <  2:1791509/150‑1 (MQ=255)
                                                   tgtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGAAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg                                                                                                 <  1:419277/150‑1 (MQ=255)
                                                     tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                               <  1:491755/150‑1 (MQ=255)
                                                       gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgaga                                                                                             >  1:682446/1‑150 (MQ=255)
                                                       gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgaga                                                                                             >  1:1399368/1‑150 (MQ=255)
                                                       gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAACCGGGTTTTTTCGAAAATTTTTTTCTGCCGGAGCATAAGCCAATTAACGTTTGgaga                                                                                             >  1:1294299/1‑150 (MQ=255)
                                                        tttttGTTTTACCTGTCTGACAGGTGGCAGCAAAAAGCAGCTTTCCAGTTTTTACGCTGATTCAGATTGTAGCTATAAAATAACCCGCCGAAGAGGGTTTTTTCGAAAATAGTTTTATGCCTGAGCAGAAGCCAATTAACGTGTGGAGaa                                                                                            >  1:1209312/1‑150 (MQ=255)
                                                         ttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATCAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAAc                                                                                           <  2:1685588/150‑1 (MQ=255)
                                                             gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                       >  1:1651077/1‑150 (MQ=255)
                                                             gTTTTACCTGTCTGTCAGGGGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                       <  2:540388/150‑1 (MQ=255)
                                                              ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCAAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTCTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg                                                                                      >  1:809047/1‑150 (MQ=255)
                                                              ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTATCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTGTGGAGAACTGCgg                                                                                      <  1:80144/150‑1 (MQ=255)
                                                                 tACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacg                                                                                   <  2:719302/150‑1 (MQ=255)
                                                                  aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgc                                                                                  >  2:1515366/1‑149 (MQ=255)
                                                                  aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacga                                                                                  >  2:1064301/1‑150 (MQ=255)
                                                                   ccTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgac                                                                                 <  1:1578450/150‑1 (MQ=255)
                                                                       tctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGacgacgacg                                                                             <  2:1789138/150‑1 (MQ=255)
                                                                         tgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTg                                                                           >  1:512099/1‑150 (MQ=255)
                                                                          gtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGc                                                                          >  2:851557/1‑150 (MQ=255)
                                                                            cAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGCCGTCCtt                                                                        >  1:483410/1‑150 (MQ=255)
                                                                             aGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCttt                                                                       >  1:465076/1‑150 (MQ=255)
                                                                              ggTGGCAGCAAAAAGCTCCTTTCCAGGTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAATATTGTTTTCTGCCGGAGCAGCAGCCAATTAACGTTTGGAGAGCTGCGGTCGACGCCTTGCTTTa                                                                      >  2:302224/1‑150 (MQ=255)
                                                                              ggTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTa                                                                      <  2:1326848/150‑1 (MQ=255)
                                                                                tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                    >  1:882921/1‑150 (MQ=255)
                                                                                tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACg                                                                    >  2:677156/1‑150 (MQ=255)
                                                                                 ggCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGc                                                                   <  2:1297424/150‑1 (MQ=255)
                                                                                    agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa                                                                <  1:1456015/150‑1 (MQ=255)
                                                                                      caAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGTAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAcc                                                              >  1:1336401/1‑150 (MQ=255)
                                                                                       aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCAGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCg                                                             >  1:1228457/1‑150 (MQ=255)
                                                                                             cAACTTTCGAGTTTTTACGCTGATTCAGATTTTGGCTAAAAAACAACCCGCCGACGCGGGTTTTTTCGAAACTTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTc                                                       <  2:327875/150‑1 (MQ=255)
                                                                                                cTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTa                                                    <  1:1456478/150‑1 (MQ=255)
                                                                                                cTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTa                                                    <  2:1170273/150‑1 (MQ=255)
                                                                                                 tttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTAc                                                   >  1:1302282/1‑150 (MQ=255)
                                                                                                  ttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACg                                                  <  1:1604213/150‑1 (MQ=255)
                                                                                                     cAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGtta                                               >  1:1884164/1‑149 (MQ=255)
                                                                                                     cAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTc                                               >  1:1795813/1‑150 (MQ=255)
                                                                                                     cAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTc                                               >  1:855888/1‑150 (MQ=255)
                                                                                                      aGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCa                                              >  2:1246532/1‑150 (MQ=255)
                                                                                                      aGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCa                                              >  2:1729800/1‑150 (MQ=255)
                                                                                                       gTTTTTACGCTGATTCAGATTTTAGCTTTAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                             >  1:567194/1‑150 (MQ=255)
                                                                                                       gTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                             >  1:1308094/1‑150 (MQ=255)
                                                                                                        tttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAc                                            <  1:1082657/150‑1 (MQ=255)
                                                                                                         ttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGCCGACGTGCTATACGCACCCCGACTTTCTTACGTTCCAcc                                           >  1:1776258/1‑150 (MQ=255)
                                                                                                         ttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAcc                                           <  2:1603997/150‑1 (MQ=255)
                                                                                                               gCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCTGCGCAGAAGCCAATTAACGTTTGGAGCACTGCGGAAGACGACGAGCTTTACGCAGACCGACTTGCTTTCGTTCAACCTCACGa                                     >  2:1277768/1‑150 (MQ=255)
                                                                                                               gCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGa                                     >  2:268551/1‑150 (MQ=255)
                                                                                                                  gATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCg                                  <  1:24622/150‑1 (MQ=255)
                                                                                                                    ttCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGCCTTTCTTACGTTCAACCTGACGAGCGTc                                >  1:852624/1‑150 (MQ=255)
                                                                                                                    ttCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTATTCTGCCGTAGCAGAAGCCAATTAACGTTTGGAGACCTGCGGACGAAGACGTTCTTTACGCAGACGGACTTTCTTACGTTCAACCTGACGAGCGTc                                >  2:1840531/1‑150 (MQ=255)
                                                                                                                       aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACg                             <  2:818100/150‑1 (MQ=255)
                                                                                                                        gATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTATTACGTTCAACCTGACGAGCGTCACGa                            >  2:1691123/1‑150 (MQ=255)
                                                                                                                         aTTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAg                           <  1:1842050/150‑1 (MQ=255)
                                                                                                                                 tataAAAAAACCCGCCGCAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAAAGTTTGGAGAACTGCGGACGACGACGTTCTTTACGCAGAACGAATTTCTTACGTTCAACCTCACTAGCGTCTCGAGTAACGAAg                   >  1:912249/1‑150 (MQ=255)
                                                                                                                                 tataAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAg                   <  1:1543767/150‑1 (MQ=255)
                                                                                                                                  tcgaaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGAATTTCTTACGTTCAACCTGAAGAGCGTCACGAGTAACGAAGc                  >  2:1474992/4‑150 (MQ=255)
                                                                                                                                   taAAAAAACCCGCCGAAGCTGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCGAATTAACGGTTGGATAACTGCGGACGACGACGTGCTTTACGAAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGcc                 <  1:911777/150‑1 (MQ=255)
                                                                                                                                   taAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGcc                 >  1:1106934/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACCTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGCGCGTCACGAGTAACGAAGCCAg               >  1:506636/1‑150 (MQ=255)
                                                                                                                                      aaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc              <  2:512099/150‑1 (MQ=255)
                                                                                                                                      aaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc              >  1:1345910/1‑150 (MQ=255)
                                                                                                                                         aaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGAGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGGGTAACGAAGCCAGCttt           >  2:1027004/1‑150 (MQ=255)
                                                                                                                                            ccgccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGAGTCACGAGTATCGACGCCAGCTTTACg        >  1:1184994/1‑150 (MQ=255)
                                                                                                                                             cgccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGc       >  1:667606/1‑150 (MQ=255)
                                                                                                                                                  aaGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTc  <  2:840250/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTC  >  NC_000913/3377624‑3377919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: