Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,406,129 A→T 5.4% E71D (GAA→GAT)  accC → acetyl‑CoA carboxylase, biotin carboxylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,406,1290AT 5.4% 411.8 / 7.3 136E71D (GAA→GAT) accCacetyl‑CoA carboxylase, biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (69/58);  new base T (6/2);  total (76/60)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

AAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGC  >  NC_000913/3405980‑3406278
                                                                                                                                                     |                                                                                                                                                     
aaaGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGaa                                                                                                                                                       >  2:1366696/1‑150 (MQ=255)
  aGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGAATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAAt                                                                                                                                                     <  2:716143/150‑1 (MQ=255)
     aCTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCAc                                                                                                                                                  >  2:1604821/1‑150 (MQ=255)
         ggCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGgc                                                                                                                                              >  2:121706/1‑150 (MQ=255)
           cATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGgcgc                                                                                                                                            <  1:265002/150‑1 (MQ=255)
           aaTCTAGACTGTCGCGGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTTTGACTGGCAGATGATACGGTCGGTATTGGCCCTGCTCCGGCAGTAAAAATTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGgctc                                                                                                                                            <  1:477548/149‑3 (MQ=255)
            aTCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGGTGATTTCAGCGgcgct                                                                                                                                           <  2:1419576/150‑2 (MQ=255)
            aTCAAGACTGTCGCGGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCa                                                                                                                                           <  2:1168576/150‑1 (MQ=255)
              cAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACCCGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTATAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGt                                                                                                                                         >  2:331213/1‑150 (MQ=255)
              cAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGt                                                                                                                                         >  1:1273400/1‑150 (MQ=255)
              cAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGt                                                                                                                                         >  2:636140/1‑150 (MQ=255)
               aaGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTa                                                                                                                                        >  2:402256/1‑150 (MQ=255)
               aaGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCGCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTa                                                                                                                                        <  2:1849794/150‑1 (MQ=255)
                   cTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCaa                                                                                                                                    <  1:797334/150‑1 (MQ=255)
                     gTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAatc                                                                                                                                  >  1:1609278/1‑150 (MQ=255)
                     gTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAatc                                                                                                                                  >  2:50879/1‑150 (MQ=255)
                             gCACTCCAGCGCGGATCGCGATCTAACACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGAAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCggg                                                                                                                          <  2:706113/150‑1 (MQ=255)
                             gCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGACATCACCGGCGCAGTAGCAATCCATCCggg                                                                                                                          >  1:148630/1‑150 (MQ=255)
                             gCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCggg                                                                                                                          >  2:717141/1‑150 (MQ=255)
                             gCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCggg                                                                                                                          >  2:1108306/1‑150 (MQ=255)
                             gCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCggg                                                                                                                          >  1:1822836/1‑150 (MQ=255)
                                cTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGACCATCCCGGCAATCATCAGCGCCGCTGAAATCCCCGGCGCAGTAGCAATCCCTCCGGGTTa                                                                                                                       >  1:1723736/1‑150 (MQ=255)
                                cTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTa                                                                                                                       <  2:791442/150‑1 (MQ=255)
                                 tCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTAc                                                                                                                      <  1:1033921/150‑1 (MQ=255)
                                      cgcgGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCtt                                                                                                                 >  1:75751/1‑150 (MQ=255)
                                      cgcgGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCtt                                                                                                                 <  2:521924/150‑1 (MQ=255)
                                        cgGATCGCTATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGAATTGGCCCTGCTCAGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTcc                                                                                                               <  2:286382/150‑1 (MQ=255)
                                        cgGATCGCGATCTAAAACACGTATTACTGGCAGATGAAATGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTcc                                                                                                               <  2:1897484/150‑1 (MQ=255)
                                        cgGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTcc                                                                                                               <  1:983110/150‑1 (MQ=255)
                                             cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTcc                                                                                                          <  1:341724/150‑1 (MQ=255)
                                             cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTcc                                                                                                          >  1:1061784/1‑150 (MQ=255)
                                              gcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCg                                                                                                         <  1:1529136/150‑1 (MQ=255)
                                              gcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCg                                                                                                         >  2:574586/1‑150 (MQ=255)
                                              gcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCg                                                                                                         >  2:864878/1‑150 (MQ=255)
                                                gATCTAAACCACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCgag                                                                                                       <  2:90263/150‑1 (MQ=255)
                                                gATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCgag                                                                                                       >  1:213387/1‑150 (MQ=255)
                                                 aTCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCgaga                                                                                                      <  1:1657106/150‑1 (MQ=255)
                                                   cTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAAc                                                                                                    <  1:1635328/150‑1 (MQ=255)
                                                    tAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACg                                                                                                   <  2:604759/150‑1 (MQ=255)
                                                      aaaCACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGcc                                                                                                 >  2:1163544/1‑150 (MQ=255)
                                                       aaCACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCa                                                                                                <  2:649386/150‑1 (MQ=255)
                                                         cacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAAc                                                                                              >  1:998729/1‑150 (MQ=255)
                                                             tATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTg                                                                                          >  2:95901/1‑150 (MQ=255)
                                                             tATTACTGGCAGATGAAACGGGCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTg                                                                                          <  1:1218123/150‑1 (MQ=255)
                                                              aTTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGc                                                                                         <  2:227840/150‑1 (MQ=255)
                                                                tACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCg                                                                                       >  1:1024538/1‑150 (MQ=255)
                                                                    ggCATATGAATCGGTCTGTATTGGCCCTGCTCCGGCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCATTCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCa                                                                                   <  2:1505054/150‑1 (MQ=255)
                                                                    ggCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCACTCCAGCCGGGTTACGGCTTCCACGCCGAGAACGCCAACTTTGCCGAGCa                                                                                   >  1:474355/1‑150 (MQ=255)
                                                                    ggCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCa                                                                                   >  2:555690/1‑150 (MQ=255)
                                                                    ggCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCCCAGTAGCAATCCATCCGGGTTCCGGCTTCCTCTCCGAGACCGCCAACTTTGCCGCGCa                                                                                   >  2:254352/1‑150 (MQ=255)
                                                                     gCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTAATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAg                                                                                  <  1:1775891/150‑1 (MQ=255)
                                                                         aTGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAACAACCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTg                                                                              >  2:1316763/1‑150 (MQ=255)
                                                                          tGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGCACGCCCACTTTGCCGAGCAGGTTGa                                                                             >  1:1197197/1‑150 (MQ=255)
                                                                                ggTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTc                                                                       >  2:1087947/1‑150 (MQ=255)
                                                                                  tCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCCATCCATCCGGGTTACGCCTTCCTATCCGAGAACGCCAACTTTGCCGAGCAGGTAGAACGCTCCg                                                                     >  2:306534/1‑150 (MQ=255)
                                                                                    tGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCACCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGCGACCGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGc                                                                   >  2:110227/1‑150 (MQ=255)
                                                                                     gTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCt                                                                  >  1:775042/1‑150 (MQ=255)
                                                                                     gTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCt                                                                  >  1:1329677/1‑150 (MQ=255)
                                                                                      tATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCtt                                                                 >  2:868211/1‑150 (MQ=255)
                                                                                          ggCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCCAC‑TTGCCGAGCAGGTTGAACGCTCCGGCTTTTTCt                                                            >  1:1156403/1‑150 (MQ=255)
                                                                                          ggCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATc                                                             >  2:248319/1‑150 (MQ=255)
                                                                                           gCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGCACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCt                                                            >  1:119169/1‑150 (MQ=255)
                                                                                            cccTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCtt                                                           >  1:1601222/1‑150 (MQ=255)
                                                                                               tGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGATACGGCTTCCTCTCCGACAACGCCAACTTTGCCTAGCAGGTTGAACGCTCCGGCTTTATCTTCaa                                                        >  1:896656/1‑149 (MQ=255)
                                                                                                 cTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTg                                                      >  1:1488769/1‑150 (MQ=255)
                                                                                                  tCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTgg                                                     <  2:133908/150‑1 (MQ=255)
                                                                                                   ccGTGAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGc                                                    >  2:465071/1‑150 (MQ=255)
                                                                                                    cGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGcc                                                   <  2:1692179/150‑1 (MQ=255)
                                                                                                     gTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCGTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGccc                                                  <  1:1002084/150‑1 (MQ=255)
                                                                                                      tCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCg                                                 >  2:828473/1‑150 (MQ=255)
                                                                                                         gTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGata                                              >  1:174901/1‑148 (MQ=255)
                                                                                                         gTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGaaa                                              >  2:1807739/1‑150 (MQ=255)
                                                                                                         gTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGaaa                                              <  1:1901289/150‑1 (MQ=255)
                                                                                                         gTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGaaa                                              >  2:1133617/1‑150 (MQ=255)
                                                                                                          tAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAg                                             <  1:351269/150‑1 (MQ=255)
                                                                                                           aaaaaGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGACCGCCAACTTTGCCGCGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAACGc                                            >  1:1169469/1‑150 (MQ=255)
                                                                                                           aaaaaGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGc                                            <  2:1235844/150‑1 (MQ=255)
                                                                                                              aaGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGa                                         <  1:135406/150‑1 (MQ=255)
                                                                                                                 ttATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAAc                                      <  1:651012/150‑1 (MQ=255)
                                                                                                                  tagcTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGCGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAAcc                                     <  2:1483296/147‑1 (MQ=255)
                                                                                                                    tCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGATAGCAGAAACCAt                                   >  2:42396/1‑150 (MQ=255)
                                                                                                                      tGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGTCCGAAAGCAGAAACCATTc                                 >  1:532802/1‑150 (MQ=255)
                                                                                                                      tGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTc                                 >  1:400906/1‑150 (MQ=255)
                                                                                                                       gAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCTAGCAGGTTGAACGCTCCGGCTTTAACTTCATTGGCCCGAAAGCAGAAACCATTCg                                >  1:179890/1‑150 (MQ=255)
                                                                                                                            tCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGa                           >  1:1255166/1‑150 (MQ=255)
                                                                                                                              ccGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATg                         >  1:628588/1‑150 (MQ=255)
                                                                                                                                ggCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATggg                       <  1:505661/150‑1 (MQ=255)
                                                                                                                                 gCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGc                      <  2:1634978/150‑1 (MQ=255)
                                                                                                                                   aaTCATCATCGCCGCTGATATCACCGGCGCAGTATCAATCAATCCGGGTTACGGCTTCCTCTCCGAGACCGCCTACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAATCAGAAACCATTCGCCTGTTGGGCGa                    <  1:325874/150‑1 (MQ=255)
                                                                                                                                     tcatcaGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACa                  <  1:1829106/150‑1 (MQ=255)
                                                                                                                                        tcaGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAg               <  2:1061784/150‑1 (MQ=255)
                                                                                                                                         caGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGt              >  2:937208/1‑150 (MQ=255)
                                                                                                                                          tgcgcCGGTGATTTCAGCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTa             >  1:51909/2‑150 (MQ=255)
                                                                                                                                          aGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTa             <  1:489701/150‑1 (MQ=255)
                                                                                                                                           gcgcCGGTGATTTCAGCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTAt            >  1:1853845/1‑150 (MQ=255)
                                                                                                                                           gcgcCGGTGATTTCAGCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTAt            >  2:1834346/1‑150 (MQ=255)
                                                                                                                                           gcgcCGGTGATTTCAGCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTAGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTAt            >  1:42539/1‑150 (MQ=255)
                                                                                                                                           gcgcCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTAt            >  1:907182/1‑150 (MQ=255)
                                                                                                                                            cgccgcTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATc           <  1:940515/150‑1 (MQ=255)
                                                                                                                                                gcTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCa       >  1:19030/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCTTTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGC  >  NC_000913/3405980‑3406278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑