Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,458,1100CA100.0% 388.1 / NA 114T578T (ACC→ACAgspDgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (58/56);  total (58/56)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 117 total aligned reads displayed.

ACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457964‑3458256
                                                                                                                                                  |                                                                                                                                                  
actaagcAAAACTCCGTGCTGGGCAACACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACagag                                                                                                                                                 <  1:344378/144‑1 (MQ=255)
aCTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACagag                                                                                                                                                 >  2:655895/1‑150 (MQ=255)
 cTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgc                                                                                                                                                >  1:921308/1‑150 (MQ=255)
 cTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgc                                                                                                                                                <  1:1377385/150‑1 (MQ=255)
     tCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcgc                                                                                                                                            <  2:845936/150‑1 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                           >  2:1285472/1‑150 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                           >  1:1906914/1‑150 (MQ=255)
      cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCGTGGCGATAATCCTTTAGTGTGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                           >  2:238893/1‑150 (MQ=255)
             ccGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCa                                                                                                                                    <  1:1003037/150‑1 (MQ=255)
             ccGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCa                                                                                                                                    <  1:845305/150‑1 (MQ=255)
                ggCTGGTCAAAACCGGTGAAACGGTGGGCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAcc                                                                                                                                 <  2:1278410/149‑1 (MQ=255)
                 gCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCt                                                                                                                                >  1:1147345/1‑150 (MQ=255)
                  cTGGTCAAAACCGGTGAAAGGGTGTTACTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCATGTGTCAAAGGTTCGTCTGCTTGGCGATATTCGTTTAGTGGGGCAACTCTTACGCTATACCTCCACAGAGCGCGGTAAACGCaaggtg                                                                                                                               <  2:1104878/150‑5 (MQ=255)
                    ggTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAt                                                                                                                             >  1:542252/1‑150 (MQ=255)
                      tCAACACCCGTGAAACGGTGGGACTGGGCGGATTGCAGGATGATTTTTCTAAAGAGCAAGTGGCAAAGGTTCCTCTGCTTGGCGAAATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATgg                                                                                                                           <  2:771112/150‑1 (MQ=255)
                      tCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATgg                                                                                                                           >  2:842615/1‑150 (MQ=255)
                      tCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATgg                                                                                                                           <  1:876149/150‑1 (MQ=255)
                      gcAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCATCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATgg                                                                                                                           <  1:523055/149‑1 (MQ=255)
                       cAAAACCGGGGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGt                                                                                                                          <  2:1441999/150‑1 (MQ=255)
                          aaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTTGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAtt                                                                                                                       <  2:697972/150‑1 (MQ=255)
                          aaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAtt                                                                                                                       <  2:57159/150‑1 (MQ=255)
                           aCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAttt                                                                                                                      >  2:690499/1‑150 (MQ=255)
                            ccGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGAAGGTATTTa                                                                                                                     >  2:957557/1‑150 (MQ=255)
                              ggTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtc                                                                                                                   <  1:1567447/150‑1 (MQ=255)
                              ggTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtc                                                                                                                   >  1:697850/1‑150 (MQ=255)
                              ggTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtc                                                                                                                   >  1:1772013/1‑150 (MQ=255)
                                tGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccg                                                                                                                 <  1:727309/150‑1 (MQ=255)
                                   aaCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCTACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgtcc                                                                                                              >  1:996757/1‑150 (MQ=255)
                                    aCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgtccg                                                                                                             <  2:751506/150‑1 (MQ=255)
                                    aCGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgtccc                                                                                                             >  2:1882956/1‑149 (MQ=255)
                                     cGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCGATACCTCCACAGCGCGCGCTAAGCGCAACCTGATGGTTTTTTtccgtccgc                                                                                                            >  2:1087356/1‑149 (MQ=255)
                                     cGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCGTTAGTGGGGCAGCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcga                                                                                                            >  2:1079763/1‑150 (MQ=255)
                                      ggtggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcgac                                                                                                           <  1:1468863/150‑1 (MQ=255)
                                       gtggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCcgacg                                                                                                          <  2:1137324/150‑1 (MQ=255)
                                         ggtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGAt                                                                                                        <  2:547308/150‑1 (MQ=255)
                                              tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTTTTTATCCGTCCGACGATTATcc                                                                                                   >  2:1692999/1‑150 (MQ=255)
                                              tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                   >  1:558509/1‑150 (MQ=255)
                                              tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAAATCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                   >  1:1038441/1‑150 (MQ=255)
                                              tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGAAAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTAAACCTCCACAGAGCGCGATAAACGCAACCTGATGGTATTTAGCCGACAGACGATTATcc                                                                                                   >  1:629254/1‑150 (MQ=255)
                                                 gCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTg                                                                                                <  2:1729425/150‑1 (MQ=255)
                                                  cGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGa                                                                                               <  2:388649/150‑1 (MQ=255)
                                                        gCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCACCTCTTACGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCGGACGATTATCCGTGACgatga                                                                                         >  2:145615/1‑150 (MQ=255)
                                                        gCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTTTACCTCCACAGAGCGCGCTAACCGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatga                                                                                         >  1:407991/1‑150 (MQ=255)
                                                            gatgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGtt                                                                                     <  1:1349508/150‑1 (MQ=255)
                                                               gatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTAt                                                                                  >  2:151595/1‑150 (MQ=255)
                                                                 tttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCg                                                                                <  2:542252/150‑1 (MQ=255)
                                                                 tttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCg                                                                                >  1:1484307/1‑150 (MQ=255)
                                                                  ttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGc                                                                               <  2:228684/150‑1 (MQ=255)
                                                                   tttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCt                                                                              <  1:1810096/150‑1 (MQ=255)
                                                                    ttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGAGGATGTTTATCGCTc                                                                             >  2:1422643/1‑150 (MQ=255)
                                                                     tCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCa                                                                            <  1:1473992/150‑1 (MQ=255)
                                                                      cTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCAc                                                                           >  1:1439734/1‑150 (MQ=255)
                                                                      cTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCAc                                                                           >  2:1633322/1‑150 (MQ=255)
                                                                      cTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTAAc                                                                           >  2:1311119/1‑150 (MQ=255)
                                                                        aaaGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTg                                                                         <  2:1147345/150‑1 (MQ=255)
                                                                           gagCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCa                                                                      >  1:1023812/1‑150 (MQ=255)
                                                                             tcAAGTGTCTAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACTCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGTTGATGTTAATCGCTCACTGTCaaa                                                                    <  1:1413094/149‑1 (MQ=255)
                                                                             gCAAGTGGCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTAAACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCaaa                                                                    <  2:201517/150‑1 (MQ=255)
                                                                              cAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGAAGATTATCCTTGACGATGATGTTTATCTCTCACTGTCaaaa                                                                   >  2:1026744/1‑150 (MQ=255)
                                                                               aaGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTGCGCTATACCTCCACAGAGCGCTCTAATCGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAag                                                                  <  2:835875/150‑1 (MQ=255)
                                                                                      aaaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCATCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATa                                                           <  2:888954/150‑1 (MQ=255)
                                                                                       aaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATac                                                          >  2:960613/1‑150 (MQ=255)
                                                                                       aaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATac                                                          <  1:690499/150‑1 (MQ=255)
                                                                                         ggTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGTTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATacac                                                        <  1:1285472/150‑1 (MQ=255)
                                                                                          gTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACAcc                                                       <  2:1023812/150‑1 (MQ=255)
                                                                                            tCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAAGGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     <  2:1293045/150‑1 (MQ=255)
                                                                                            tCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     <  1:1764314/150‑1 (MQ=255)
                                                                                                 tGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTAcc                                                >  2:1759/1‑150 (MQ=255)
                                                                                                  gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCg                                               <  1:1318736/150‑1 (MQ=255)
                                                                                                  gCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACATGATGGTATTTTTCCGTCCGACGATTATCCGTGCCGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCg                                               >  2:266400/1‑150 (MQ=255)
                                                                                                     tGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGTGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCa                                            >  1:1411440/1‑150 (MQ=255)
                                                                                                        cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         >  1:766230/1‑150 (MQ=255)
                                                                                                          atatTCCTTAAGTGGGGCACCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGc                                       <  2:158946/150‑1 (MQ=255)
                                                                                                           tatTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGca                                      <  1:1293440/150‑1 (MQ=255)
                                                                                                           tatTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGca                                      <  1:1585299/150‑1 (MQ=255)
                                                                                                            atTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaa                                     <  2:888083/150‑1 (MQ=255)
                                                                                                             ttCCTTTAGTGGGGCAACTCTTCCTCTATACCTCCAAAGAGCGCGCTAAACGCAACCTGATGGTATTAATCCGTCAGACGATTATCCGTGACGATGATGTTTATCGCTCACTGGCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaac                                    <  1:288534/150‑1 (MQ=255)
                                                                                                               ccTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaacaa                                  >  2:33945/1‑150 (MQ=255)
                                                                                                               ccTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACACAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGcaacaa                                  >  1:1496010/1‑150 (MQ=255)
                                                                                                                   tAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCTGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGc                              <  1:1118769/150‑1 (MQ=255)
                                                                                                                      tGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGa                           >  2:850762/1‑150 (MQ=255)
                                                                                                                       ggggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGAt                          >  1:1088234/1‑150 (MQ=255)
                                                                                                                       ggggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGAt                          >  2:1615226/1‑150 (MQ=255)
                                                                                                                       ggggCAACTCTTCCGCTATACCTCCACAGAGCGCGCGAAACGCAACCTGATGGTATATATCCGTACTACGATTATCCGTGACGATGATGTTTATCGCTCAAAGTCAAAAGAGAAAGACACCCGTTCCCGGCCGGAGCAACCACCGCGGag                          >  2:1731165/1‑149 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAGCGCAACCTGATGGTATTTATCCGTCCGACGATTATACGTGACGATGATTTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                        >  2:891470/1‑150 (MQ=255)
                                                                                                                         ggCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCg                        >  1:1859950/1‑150 (MQ=255)
                                                                                                                            aaCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAATAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACg                     <  1:198080/150‑1 (MQ=255)
                                                                                                                              ctctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACggg                   >  1:228329/1‑150 (MQ=255)
                                                                                                                              ctctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGGTACCGTCAGGAGCAACAACAGCGGATCGACGGGa                  >  2:1741943/1‑150 (MQ=255)
                                                                                                                                ctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGaa                 >  1:1073195/1‑150 (MQ=255)
                                                                                                                                   ccGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATc              <  1:439467/150‑1 (MQ=255)
                                                                                                                                     gCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaa            <  1:27307/150‑1 (MQ=255)
                                                                                                                                         taCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgc        >  1:192597/1‑150 (MQ=255)
                                                                                                                                           ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCGGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcgc      >  2:1743841/1‑150 (MQ=255)
                                                                                                                                           ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcgc      >  1:1430533/1‑150 (MQ=255)
                                                                                                                                           ccTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgcgc      >  1:1693918/1‑150 (MQ=255)
                                                                                                                                              ccACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAATACAAAAGCGCTgg   >  1:931136/1‑150 (MQ=255)
                                                                                                                                              ccACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTgg   >  1:1778255/1‑150 (MQ=255)
                                                                                                                                              ccACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTgg   >  2:1052793/1‑150 (MQ=255)
                                                                                                                                               cacaGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCTACGGGAAATCAAAAGCGCTGGt  >  1:428179/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457964‑3458256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: