Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,486,218 G→T 5.4% Q33H (CAG→CAT) ‡ crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,2180GT 5.4% 309.9 / 5.5 104Q33H (CAG→CAT) ‡crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base G (40/58);  new base T (3/3);  total (43/61)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 110 total aligned reads displayed.

CAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACG  >  NC_000913/3486069‑3486367
                                                                                                                                                     |                                                                                                                                                     
cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCCAGAGCACGCTTATTCACCAg                                                                                                                                                       >  1:1027928/1‑150 (MQ=255)
cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAg                                                                                                                                                       <  1:422936/150‑1 (MQ=255)
 aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGTATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                                                                                                                      <  1:1459389/150‑1 (MQ=255)
 aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                                                                                                                      >  1:1454740/1‑150 (MQ=255)
 aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGAAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAgg                                                                                                                                                      <  2:1604200/150‑1 (MQ=255)
     gcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGTATAACCGCTCAAGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGa                                                                                                                                                  <  1:1031793/150‑1 (MQ=255)
       tttATGTGGCTCTGGAGAAAGCTTATAAAAGCGGATAACCGCGCATGGGGCTTGTAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACTCTTATTCACCAGGGTGaaa                                                                                                                                                <  1:1740998/149‑1 (MQ=255)
         tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                                                                                                                              >  1:1318982/1‑150 (MQ=255)
             ttgCTCTGGAGAAAGCTTATAACAGAGGAAAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCgg                                                                                                                                          <  1:1389690/148‑1 (MQ=255)
             tGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCgg                                                                                                                                          <  1:697356/150‑1 (MQ=255)
              ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                                                                                                                         <  1:1279592/150‑1 (MQ=255)
               gcactGGAGAAAGCTTACAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCCAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                                                        <  2:302381/147‑1 (MQ=255)
               gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                                                        <  2:22046/150‑1 (MQ=255)
               gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                                                        >  1:859574/1‑150 (MQ=255)
               gCTCTGGAGAAAGCTTAAAACAGCGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTTTTCACCCTGGTGAATAAGCgtgc                                                                                                                                        <  2:398692/150‑4 (MQ=255)
                ctctGGAGAAAGCTTATAACAGCGTATAACCGCGCATGGTGCTTGGCAAACCGCAAAAAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCACGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa                                                                                                                                       <  1:230591/150‑1 (MQ=255)
                 tctGGAGAAAGCTTATAACAGAGTATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTTTTCACCCTGGTGAATAAGCgtgctc                                                                                                                                      <  1:677164/150‑6 (MQ=255)
                   tGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCACACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                                                                                                                                    >  2:1333780/1‑150 (MQ=255)
                      agaATGCTTATAACAGAGCATAACCGCGCATTGGGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                 <  2:961418/150‑1 (MQ=255)
                      agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                 <  2:423524/150‑1 (MQ=255)
                       gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta                                                                                                                                >  1:186325/1‑150 (MQ=255)
                            gttATAACTGAGGAAAACCGCGCATGGTGGTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGGTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                                                                                                                           <  2:1155482/149‑1 (MQ=255)
                            cTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                                                                                                                           >  1:1111307/1‑150 (MQ=255)
                             ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                                                          <  2:1522329/150‑1 (MQ=255)
                             ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                                                          <  1:1765848/150‑1 (MQ=255)
                               ataACCGCGTATAACCGCGCAGGGTGCTTGGCAAACCTCACACAGACCCGACTCTCGAATGGTTCTTGTCTCCTGGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                                                                                                                        <  1:1222086/150‑1 (MQ=255)
                               ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                                                                                                                        <  2:848121/150‑1 (MQ=255)
                                  aCAGATGATAACCGCGCATGGTGCTTGGCAAACCGCAAACCGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                                                     <  2:736103/150‑1 (MQ=255)
                                  aCAGAGGATAAACGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACAATCATAAGTACACATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCTGAAACGCTGTACTACATCGTTa                                                                                                                     >  2:1685207/1‑150 (MQ=255)
                                   cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                                                    >  2:1416869/1‑150 (MQ=255)
                                   cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGATCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                                                    <  2:1339832/150‑1 (MQ=255)
                                     gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAg                                                                                                                  <  2:972665/150‑1 (MQ=255)
                                       ggATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc                                                                                                                <  2:746687/150‑1 (MQ=255)
                                        gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCGCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGct                                                                                                               <  2:1220699/150‑1 (MQ=255)
                                               gcgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc                                                                                                        <  1:903339/150‑1 (MQ=255)
                                                 gcATGGTGCTTTGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAg                                                                                                      <  1:685015/150‑1 (MQ=255)
                                                    tGGTGCTTGGCAATCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTCTACTACATCGTTAATGGCTCTGTGGCAGTGc                                                                                                   >  2:495217/1‑150 (MQ=255)
                                                    tGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTAACCAGCGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGATCTGTGGAAgatc                                                                                                   >  2:1352436/1‑147 (MQ=255)
                                                     ggTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACTTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                                                  <  1:1232068/150‑1 (MQ=255)
                                                      gTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTg                                                                                                 <  2:1373516/150‑1 (MQ=255)
                                                         cTTGTCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                                                              <  2:516923/150‑1 (MQ=255)
                                                            ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa                                                                                           >  1:1296677/1‑150 (MQ=255)
                                                             gCAAACCGCAAACAGACCCGACTCTCGAATGGTGCTTGTCTAATTGCAACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAg                                                                                          <  2:1074073/150‑1 (MQ=255)
                                                              cAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGa                                                                                         >  1:62321/1‑150 (MQ=255)
                                                              cAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGa                                                                                         <  2:1755571/150‑1 (MQ=255)
                                                               aaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTTATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                                                        >  1:970842/1‑150 (MQ=255)
                                                               aaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                                                        <  2:1537131/150‑1 (MQ=255)
                                                                 aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                                                      >  1:1037853/1‑150 (MQ=255)
                                                                 aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                                                      >  1:1073088/1‑150 (MQ=255)
                                                                  ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTAACCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGaa                                                                                     >  2:576459/1‑150 (MQ=255)
                                                                    ccAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAaga                                                                                   <  1:1284498/149‑1 (MQ=255)
                                                                     cacaCAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                                                  <  2:1701628/147‑1 (MQ=255)
                                                                     cAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                                                  >  1:1282816/1‑150 (MQ=255)
                                                                     cAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                                                  >  2:921958/1‑150 (MQ=255)
                                                                       aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagagtt                                                                                <  2:172954/150‑3 (MQ=255)
                                                                       aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg                                                                                <  1:963889/150‑1 (MQ=255)
                                                                       aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg                                                                                <  2:631375/150‑1 (MQ=255)
                                                                       aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg                                                                                <  1:814631/150‑1 (MQ=255)
                                                                             cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGa                                                                          <  2:1629138/150‑1 (MQ=255)
                                                                               cGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaaa                                                                        >  2:1329661/1‑150 (MQ=255)
                                                                                  ctctCGAATGGTTATTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGTGTGAAAAAGAGGAAACTCTGTACTACATCGTTAAATGCTCTGTGGCAGTTCTGATCAAAGACGAAGAGGGTAAAGAAATGa                                                                     <  1:46038/150‑1 (MQ=255)
                                                                                   taaagAATGGTTCTTGTCTCAATGCCCCATTCATAACGACCCATCCCAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTAATACATCGTTAAAGCCCCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGAt                                                                    <  2:291228/146‑1 (MQ=255)
                                                                                     tcGAATGGTTGTTGTCTCATTGCCACTATCATAAGTACCCATCCAAGAGCACGCTTATTCACCCGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                                                  <  2:1435686/150‑1 (MQ=255)
                                                                                      cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAGTGCTCct                                                                 >  1:90121/1‑150 (MQ=255)
                                                                                               cTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                                                        <  1:563398/150‑1 (MQ=255)
                                                                                               cTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                                                        <  2:235392/150‑1 (MQ=255)
                                                                                                   tctcATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAAt                                                    <  2:1186297/150‑1 (MQ=255)
                                                                                                    ctcATTGCCACATTCATAAGTACCCATCAAAGAGCACGCTTATTCACAAGAGTGAAAAAGCGGAGACGCTGTCCTACATCGTTAAAGGCTCTGTCGCAGAGCTGATCAAAGCGTCAGAGGGTAAAGAAATGATCCTCTCCTATCTGCATc                                                   >  1:275180/1‑150 (MQ=255)
                                                                                                      cATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAg                                                 >  1:1672690/1‑150 (MQ=255)
                                                                                                        ttGCCACATTCATAAGTACCCCTCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAggg                                               <  1:1375378/150‑1 (MQ=255)
                                                                                                        ttGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAggg                                               <  1:1708006/150‑1 (MQ=255)
                                                                                                        ttGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAggg                                               <  2:7494/150‑1 (MQ=255)
                                                                                                          gCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTg                                             >  1:1296422/1‑150 (MQ=255)
                                                                                                          gCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTg                                             >  1:429052/1‑150 (MQ=255)
                                                                                                            cacaTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAt                                           >  1:1685168/1‑150 (MQ=255)
                                                                                                              aaTTCATAAGTACACATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAttt                                         <  1:1256913/149‑1 (MQ=255)
                                                                                                                 tCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAt                                      >  1:1090596/1‑150 (MQ=255)
                                                                                                                  cATAAGTACCCATCCAAGAGCACGCTTATTCTCCAGGGGGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGTTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                     <  2:755925/150‑1 (MQ=255)
                                                                                                                    tAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTgg                                   <  2:186325/150‑1 (MQ=255)
                                                                                                                     aaGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGTAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc                                  <  1:956449/150‑1 (MQ=255)
                                                                                                                      aGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCg                                 >  2:1502190/1‑150 (MQ=255)
                                                                                                                          cccATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACt                             >  2:1599623/1‑150 (MQ=255)
                                                                                                                           ccATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTg                            <  2:653357/150‑1 (MQ=255)
                                                                                                                            cATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGTCGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                           >  2:1759114/1‑150 (MQ=255)
                                                                                                                               ccAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGcc                        >  1:491963/1‑150 (MQ=255)
                                                                                                                               ccAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGcc                        <  2:811492/150‑1 (MQ=255)
                                                                                                                                    agCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCGTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGttt                   >  1:356227/1‑150 (MQ=255)
                                                                                                                                     ccACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTg                  <  2:255372/149‑1 (MQ=255)
                                                                                                                                       ccGCTTTTTCACCCTGGTGAATAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGaa                >  1:1838235/2‑150 (MQ=255)
                                                                                                                                       aCGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGaa                <  1:1722395/150‑1 (MQ=255)
                                                                                                                                        gtcTTATTCACCAGGGTGAAAAAGCTGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag               <  2:768072/148‑1 (MQ=255)
                                                                                                                                        cGCTTTTTCACCCTGGTGAATAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGCTTTTATTGGCGAACTGGGCCTGTTTGaat               >  2:1374092/1‑149 (MQ=255)
                                                                                                                                        cGCTTTTTCACCCTGGTGAATAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag               >  2:27324/1‑150 (MQ=255)
                                                                                                                                        cGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag               >  2:752870/1‑150 (MQ=255)
                                                                                                                                           ttATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAgg            <  2:1393516/150‑1 (MQ=255)
                                                                                                                                            tATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAggg           >  2:557044/1‑150 (MQ=255)
                                                                                                                                               tCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGTGAACTGGGCCTGTTTGAAGAGGGCCa        >  1:784013/1‑150 (MQ=255)
                                                                                                                                                  ccAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGa     >  1:1229226/1‑150 (MQ=255)
                                                                                                                                                   cAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGCCCTCTTCAAACAGGCCCAgttc    <  1:827928/150‑4 (MQ=255)
                                                                                                                                                     gggTGAAAAAGCGTATACTGTGTACTTCATCGTTTAAGGCTCTGTGTCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTTAATGAGGGTGATTTTATTGGCGAACTTGGCCTGTTTGAAGAGGGCCAGGAACg  <  2:923731/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACG  >  NC_000913/3486069‑3486367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: