Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 265,583 A→G 7.3% intergenic (‑40/+37) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,5830AG 7.3% 364.6 / 14.2 123intergenic (‑40/+37)ykfF/ykfBCP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (58/56);  new base G (6/3);  total (64/59)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 124 total aligned reads displayed.

AGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCT  >  NC_000913/265435‑265732
                                                                                                                                                    |                                                                                                                                                     
   gACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCGGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTg                                                                                                                                                   >  1:735452/1‑150 (MQ=255)
   gACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTAGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTg                                                                                                                                                   >  1:1074164/1‑150 (MQ=255)
        cccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                              <  1:1384139/150‑1 (MQ=255)
        cccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGGGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                              <  1:1713509/150‑1 (MQ=255)
         ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCgggg                                                                                                                                             <  1:1304482/150‑1 (MQ=255)
         ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTAGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGACgggg                                                                                                                                             >  2:370103/1‑150 (MQ=255)
           tGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTg                                                                                                                                           <  2:1145924/150‑1 (MQ=255)
           tGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTg                                                                                                                                           <  2:1078076/150‑1 (MQ=255)
           tGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCGGGGtt                                                                                                                                           <  1:819342/150‑2 (MQ=255)
           cggTCGTCTTCGAGTGTGATATTGCTGTACGTGGAAGTGACCGCTTGCGCTTGGCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGAAAAAAGAAAACCCCGACAGCTAGCAGGCTGCCGGGGtt                                                                                                                                           <  2:771729/149‑2 (MQ=255)
               cgtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAtt                                                                                                                                       <  1:541090/150‑1 (MQ=255)
               cggcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAtt                                                                                                                                       <  1:975464/147‑1 (MQ=255)
                gtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAACTGTCGGGGTGAAttt                                                                                                                                      >  1:1743929/1‑150 (MQ=255)
                 tcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTg                                                                                                                                     >  1:1068752/1‑150 (MQ=255)
                  cTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGc                                                                                                                                    >  2:1524383/1‑150 (MQ=255)
                        gtgtGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGa                                                                                                                              >  1:32443/1‑150 (MQ=255)
                        gtgtGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTAGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCAAGCTGTCGGGGTGAATTTGCTGGGGa                                                                                                                              >  2:914510/1‑150 (MQ=255)
                          gtgATATTGCTGTACGTGGTAGTGACCGCTTGCGCATGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAg                                                                                                                            <  1:818499/150‑1 (MQ=255)
                           tgATATTGCTGTACGTGGTAGTGCCCGCTTGCGCTTGTCTCCGTGTGAAATGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCATGATAAAAGAAAACCCCGGCAGCCTGCTAGATGTCGGGGTGAAGTTTCTGGGTAAGa                                                                                                                           >  1:403218/1‑150 (MQ=255)
                           tgATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGTCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGa                                                                                                                           <  1:1675134/150‑1 (MQ=255)
                            gATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAt                                                                                                                          >  2:1749004/1‑150 (MQ=255)
                             atatTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAta                                                                                                                         >  1:1522735/1‑150 (MQ=255)
                                ttGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                                      >  2:1750199/1‑150 (MQ=255)
                                 tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtactac                                                                                                                     >  2:1482863/1‑150 (MQ=255)
                                  gCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                       <  2:1140233/147‑1 (MQ=255)
                                  gCTGCACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                       <  2:909403/147‑1 (MQ=255)
                                  gCTGCACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                       >  1:909403/1‑147 (MQ=255)
                                   cTGTACGTGGTAGTGACCGCTTGCGCTGGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATCGGGCCAGTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATActacta                                                                                                                   <  1:138829/150‑1 (MQ=255)
                                    tGTACTTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGTCCTCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAt                                                                                                                  <  2:174664/150‑1 (MQ=255)
                                     gTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATc                                                                                                                 <  1:785353/150‑1 (MQ=255)
                                      tACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCa                                                                                                                <  1:1540458/150‑1 (MQ=255)
                                        cGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGt                                                                                                              >  1:1073260/1‑150 (MQ=255)
                                         gTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTc                                                                                                             >  1:882125/1‑150 (MQ=255)
                                          tGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGATGGGGAAGATACTACTATCAGTCg                                                                                                            >  2:170432/1‑150 (MQ=255)
                                            gTAGTTACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGtt                                                                                                          <  1:394940/150‑1 (MQ=255)
                                            gTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGtt                                                                                                          <  2:1169749/150‑1 (MQ=255)
                                              aGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgc                                                                                                        <  1:209643/150‑1 (MQ=255)
                                              aGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgc                                                                                                        <  1:1608442/150‑1 (MQ=255)
                                               gTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGGTAGCTGTCGGGGTGAATTTGCTGGGGAAGAAACTACTATCAGTCGTtgct                                                                                                       >  1:1518160/1‑150 (MQ=255)
                                               gTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgct                                                                                                       >  1:1155970/1‑150 (MQ=255)
                                                 gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                                     >  1:507113/1‑150 (MQ=255)
                                                 gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                                     <  2:44073/150‑1 (MQ=255)
                                                   ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAg                                                                                                   <  2:410653/150‑1 (MQ=255)
                                                        tgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTcc                                                                                              >  1:626913/1‑150 (MQ=255)
                                                           gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCgag                                                                                           >  2:536819/1‑150 (MQ=255)
                                                                tctcCGGGCGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTgg                                                                                      <  1:1207861/150‑1 (MQ=255)
                                                                 ctcCGGGTGAAAGGCCCCGTTAGCAGCAACACGGATTGGTTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                                     <  1:50883/150‑1 (MQ=255)
                                                                   ccGGGGGAACGGCCCCGGTGGCCGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCGGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACa                                                                                   <  1:1381134/150‑1 (MQ=255)
                                                                     gggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGa                                                                                 >  2:1714973/1‑150 (MQ=255)
                                                                      ggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCTGTCTCCGAGAGTGGACAGaa                                                                                >  2:1797601/1‑150 (MQ=255)
                                                                        tGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACt                                                                              >  1:1224054/1‑150 (MQ=255)
                                                                              gCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCa                                                                        <  2:230260/150‑1 (MQ=255)
                                                                               ccccGGTGGCAGCAACACGGATTGGGTCATTTTCTGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGCGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAg                                                                       >  2:1398305/1‑150 (MQ=255)
                                                                               ccccGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAg                                                                       <  1:1595195/150‑1 (MQ=255)
                                                                                 ccGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCg                                                                     <  2:134235/150‑1 (MQ=255)
                                                                                    gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttc                                                                  <  2:612438/150‑1 (MQ=255)
                                                                                       gcagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttcttc                                                               <  2:624123/150‑1 (MQ=255)
                                                                                        cagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGGTGCAGTCTCCGAGAGTGGCCAGAACTTTCTCAGCGTTCTTcc                                                              >  2:1246424/1‑150 (MQ=255)
                                                                                             acacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTAAGCGTTCTTCCGGTcc                                                         >  2:1383692/1‑150 (MQ=255)
                                                                                                    ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTTGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGa                                                  <  1:291218/150‑1 (MQ=255)
                                                                                                       ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAgg                                               >  2:990861/1‑150 (MQ=255)
                                                                                                       ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAgg                                               >  1:925525/1‑150 (MQ=255)
                                                                                                       ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAgg                                               <  2:1053610/150‑1 (MQ=255)
                                                                                                            ttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTcccg                                          >  2:1625102/1‑149 (MQ=255)
                                                                                                             tttCGGGCTCCTGATAACAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCCCCGAGAGTGGACAGAACTTTCTCAGCGTGCTTCCGGTCCGCAGTGAAGGTCCCTg                                         <  2:245998/150‑1 (MQ=255)
                                                                                                               tCGGGCTCCTGATACAAGACAACACCGGCAGCCTGCTAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCt                                       <  1:661091/150‑1 (MQ=255)
                                                                                                                     tCCTGATAAAAGAAAAGCGCGGCATCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACATAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                                 <  2:1225240/150‑1 (MQ=255)
                                                                                                                     tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                                 <  2:1219043/150‑1 (MQ=255)
                                                                                                                      ccTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGt                                <  1:1000496/150‑1 (MQ=255)
                                                                                                                         gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAt                             >  1:1897687/1‑150 (MQ=255)
                                                                                                                         gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAt                             >  1:481825/1‑150 (MQ=255)
                                                                                                                         gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCTGCTTTGTGGTCAtt                            <  2:1122471/150‑1 (MQ=255)
                                                                                                                           tAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGGCCCTGCTTTGTGGTCATTg                           >  2:216402/1‑150 (MQ=255)
                                                                                                                            aaaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGa                          <  2:1698646/150‑1 (MQ=255)
                                                                                                                             aaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                         >  2:556591/1‑150 (MQ=255)
                                                                                                                              aaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACg                        <  1:602306/150‑1 (MQ=255)
                                                                                                                               aGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGt                       <  2:620747/150‑1 (MQ=255)
                                                                                                                                caaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTa                      <  1:804187/149‑1 (MQ=255)
                                                                                                                                 aaaaCCCGGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGTGAGTGGACAGAACTTTCTCAGCGTTCTTCGGGTCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg                     <  2:1518160/150‑1 (MQ=255)
                                                                                                                                 aaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGGCAGAACTTTCTCAGCGGTCTTCCGGTCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACGTat                     >  2:942138/1‑149 (MQ=255)
                                                                                                                                 aaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg                     <  2:1315441/150‑1 (MQ=255)
                                                                                                                                 aaaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg                     <  1:536819/150‑1 (MQ=255)
                                                                                                                                  tcaCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGa                    >  1:695634/3‑150 (MQ=255)
                                                                                                                                  tcaCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGa                    >  1:702291/3‑150 (MQ=255)
                                                                                                                                  aaaCCCCGGCAGCCTGCTAGCGGGCGGGGTGAATGTGCTGGGGAAGAAACTACTATCAGGCGTTGCGGCAGTCGCCGAGAGTGGACAGAACTTTCTCAGCGTTCATCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGa                    <  2:225301/150‑1 (MQ=255)
                                                                                                                                   caCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc                   >  2:58910/2‑150 (MQ=255)
                                                                                                                                    aCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACg                  >  1:435373/1‑150 (MQ=255)
                                                                                                                                     ccccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATAATACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt                 >  2:1257139/1‑150 (MQ=255)
                                                                                                                                     ccccGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt                 >  1:488240/1‑150 (MQ=255)
                                                                                                                                     ccccGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt                 >  2:1756872/1‑150 (MQ=255)
                                                                                                                                      cccGTCAGCCTGCTAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTc                <  1:914510/150‑1 (MQ=255)
                                                                                                                                       ccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg               <  2:1019647/150‑1 (MQ=255)
                                                                                                                                       ccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg               >  1:156399/1‑150 (MQ=255)
                                                                                                                                          gCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAAc            <  2:735452/150‑1 (MQ=255)
                                                                                                                                            aGCCTGCTAGCTGTGGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGAGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTCTACGTcaatgac          <  2:323291/150‑7 (MQ=255)
                                                                                                                                             gCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGc         >  1:1138/1‑150 (MQ=255)
                                                                                                                                               cTGCTAGCTGGCGGGGTGAATTTGCTGGGGACGACACTACTATCAGTCGATGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGccc       <  1:193819/150‑1 (MQ=255)
                                                                                                                                                 gCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCgg     >  1:1420530/1‑150 (MQ=255)
                                                                                                                                                 gCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGGCGTTGCTGCAGGCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCgg     <  2:1708673/150‑1 (MQ=255)
                                                                                                                                                    aGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCt  <  2:1074164/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCT  >  NC_000913/265435‑265732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: