Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,704 G→T 5.9% intergenic (‑84/+21) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7040GT 5.9% 237.2 / 3.2 95intergenic (‑84/+21)yhfG/ppiAputative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base G (25/63);  new base T (2/3);  total (29/66)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

CTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491555‑3491852
                                                                                                                                                      |                                                                                                                                                    
cTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAg                                                                                                                                                      <  1:1242407/150‑1 (MQ=255)
 tGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGa                                                                                                                                                     >  1:110047/1‑150 (MQ=255)
  gAAAATTACGCTTACGCGGTAGCTCCGAGAGAGGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAt                                                                                                                                                    <  1:269792/150‑1 (MQ=255)
  gAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCTGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAAGAt                                                                                                                                                    >  1:905315/1‑150 (MQ=255)
    aaaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTg                                                                                                                                                  <  2:216113/150‑1 (MQ=255)
    aaaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTg                                                                                                                                                  <  1:2899/150‑1 (MQ=255)
     aaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGc                                                                                                                                                 >  2:221406/1‑150 (MQ=255)
       ttacgattacgCTGAAGCTCCCAGAGACGGAACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGccc                                                                                                                                               <  2:1623213/150‑1 (MQ=255)
        tacgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcg                                                                                                                                              >  1:314579/1‑150 (MQ=255)
          cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCCGGGAAAAATTcccccc                                                                                                                                             >  2:1660665/1‑148 (MQ=255)
          cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcg                                                                                                                                            <  1:378250/150‑1 (MQ=255)
          cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTACACTTGATGCCTCCCTGAACGTCTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcg                                                                                                                                            >  1:1300576/1‑150 (MQ=255)
          cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGATTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTTCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcg                                                                                                                                            <  2:1104574/150‑1 (MQ=255)
            attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCTCGGGCAATCTTGCCcgcgct                                                                                                                                          <  1:1037094/150‑2 (MQ=255)
            attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcgcg                                                                                                                                          <  2:228941/150‑1 (MQ=255)
              tacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCgag                                                                                                                                        <  2:429793/150‑1 (MQ=255)
              accgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCgag                                                                                                                                        <  1:1776276/148‑1 (MQ=255)
               acgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGGGAGTGGCTTCACTGGATGCCTCCCTTACCGTGCCCATTTCCCCCAATTATAAGCGGCAAATCCGGGTAACGCCGGGAGAGCATAAGCGCGGGCAAGATTGCCCGCGCgaga                                                                                                                                       <  1:1703665/150‑1 (MQ=255)
                   tGAAGCTCCCAGACCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTCCCACAAGTATAATCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATc                                                                                                                                   <  1:496036/150‑1 (MQ=255)
                   ccaaGCTCCCAGAGACGGGACTTTTGCAAATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATATGCCCCAAGAATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATc                                                                                                                                   <  1:1898870/148‑1 (MQ=255)
                    gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCa                                                                                                                                  <  2:20444/150‑1 (MQ=255)
                    gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCa                                                                                                                                  <  1:1024200/150‑1 (MQ=255)
                    gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATACGGGTTACGCGGGGAGATCAGAAGCGCGTTCAAGCTTGCCCGCGCGATAACTCa                                                                                                                                  >  2:278604/1‑150 (MQ=255)
                     atgcgcccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGACCATTTGCCACAATTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAt                                                                                                                                 <  1:922388/145‑1 (MQ=255)
                     aaGCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTTATGCCTCCCTGAACGTGCCCATTTGCCACAAGAATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAt                                                                                                                                 <  1:1592048/150‑1 (MQ=255)
                      aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAtt                                                                                                                                <  2:529839/150‑1 (MQ=255)
                      aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTGACGCCGGGAGAGCAGAAGCGCGGGCAAGACTGCCCGCGCGAGAAATCAtt                                                                                                                                <  1:1540235/150‑1 (MQ=255)
                             agagACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAAATTTCCCGCGCGAGAAATAATTACGGCAg                                                                                                                         >  1:12542/1‑150 (MQ=255)
                                    ggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCGAGATTTACCGCGGCAGCAATCATTTCGGCgggccgttt                                                                                                                  >  1:907583/1‑144 (MQ=255)
                                     gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg                                                                                                                 <  1:473583/150‑1 (MQ=255)
                                     gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg                                                                                                                 <  1:1847590/150‑1 (MQ=255)
                                        ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                              <  2:1647129/150‑1 (MQ=255)
                                            gCTTATCGGTGTGTTTATACTCTTGATGCCTCCCTGAACGTTTCCATTTGCCACATCTCTAAGCGGCTAATCCGGGTTACGCAGGGAGAGCAGAAGCGCGGGCAAGTTTGCCCGCGCGAGAAATCATTACGGCAGTACTTTAGCGGAAAg                                                                                                          <  2:1388411/150‑1 (MQ=255)
                                            gCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAATCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAg                                                                                                          <  2:1550876/150‑1 (MQ=255)
                                            gCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAg                                                                                                          <  1:1524231/150‑1 (MQ=255)
                                             cTTATCGGTGAGGTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGACGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAgg                                                                                                         <  1:227973/150‑1 (MQ=255)
                                              ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                        <  2:1579494/150‑1 (MQ=255)
                                              ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTACCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                        >  2:1368098/1‑150 (MQ=255)
                                               tATCGGTGAGTTTCTTCACTTGCTGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGCTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCCCGAGAAATCATTACCGCCGGACTTTTGCGGAAAGGaa                                                                                                       >  2:991956/1‑149 (MQ=255)
                                               tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCCGGCAAGCATGGCCGCCCGGCAAATAATTAATGGCGGCCTTTAGGGGAAAgggt                                                                                                       >  1:1867058/1‑148 (MQ=255)
                                                  gggTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAc                                                                                                    <  2:1601288/149‑1 (MQ=255)
                                                  cGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAc                                                                                                    <  1:605403/150‑1 (MQ=255)
                                                  cGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAc                                                                                                    <  2:1342982/150‑1 (MQ=255)
                                                      gAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGGGAGAAATCATTACGGCAGGACGTTTGCGGAAAGGATAACGAcc                                                                                                >  2:94801/1‑150 (MQ=255)
                                                       aGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCg                                                                                               <  2:486727/150‑1 (MQ=255)
                                                        gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATATGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGCAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAATACGGCAGGACTTTAGCGGAAAGGATAACTGCCgg                                                                                              >  1:1222643/1‑150 (MQ=255)
                                                          ttcttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGtt                                                                                            <  1:1076601/150‑1 (MQ=255)
                                                            cttcACTTGATGCCGCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGtttt                                                                                          <  1:602319/150‑1 (MQ=255)
                                                               cACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAATACGGCAGGACTTTAGCGGAAAGGAAAACTACCGGTTTTGAc                                                                                       >  2:14398/1‑150 (MQ=255)
                                                                   tGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                   <  2:314579/150‑1 (MQ=255)
                                                                   tGAAGCCTCCCTTAACGTGCCCATTTGCCACAAGTATCAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                   <  2:1143318/150‑1 (MQ=255)
                                                                           cccTGAACGTGTCCTTGTGCCTCAAGTCTAAGCGGCAAATCCTGGTTACGCCGGGAGAGCAGAAGCGCTGGCAAGATTGCCCGCGCGAGAATTCATTAAGGCAGGACTTTAGCGTAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  1:1176467/150‑1 (MQ=255)
                                                                           cccTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCTGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  1:1175320/150‑1 (MQ=255)
                                                                           cccTGAACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  2:877065/150‑1 (MQ=255)
                                                                           cccTGAACGGGGCCATTTGCCACCAGTATAAGCGGCAAATCCGGGCTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGACAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  1:519440/150‑1 (MQ=255)
                                                                               caaCGTGTCCAATTGCCACAAGTAAAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACg                                                                       <  2:1670556/149‑1 (MQ=255)
                                                                                 aCGTGTCCATTTGCCACAAGTATAAGCGGCAACTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGa                                                                     <  2:86706/150‑1 (MQ=255)
                                                                                    tgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTGACGCGCGACAAAAAAATTATGCAGGTCATTATGGGAGACGATAACAACCCGGTTTGACGGCCCCTTGAGGTACgggacc                                                                  >  2:924721/1‑146 (MQ=255)
                                                                                    tgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCa                                                                  >  1:1359855/1‑150 (MQ=255)
                                                                                           ttGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCAt                                                           <  2:845286/150‑1 (MQ=255)
                                                                                             gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTTCCCGCGCGAGAAAACATTACGGCAGGTCATTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGa                                                         >  1:1258614/1‑150 (MQ=255)
                                                                                              ccACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAGTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAg                                                        <  2:104893/150‑1 (MQ=255)
                                                                                               cacaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGGTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGt                                                       <  2:389697/150‑1 (MQ=255)
                                                                                                  caGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTGTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCgg                                                    <  1:1406631/149‑1 (MQ=255)
                                                                                                   aGTATAAGCGTCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGTGCAAGATTGCCCGCGCGAGAAATCATTACGTCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGc                                                   <  2:1338989/150‑1 (MQ=255)
                                                                                                        aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg                                              <  2:220315/150‑1 (MQ=255)
                                                                                                               aaaTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGATAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATc                                       <  2:1005198/150‑1 (MQ=255)
                                                                                                                aaTCCGGGTTACGCCGGGAGCGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCt                                      <  1:377545/150‑1 (MQ=255)
                                                                                                                aaTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCt                                      <  2:1106262/150‑1 (MQ=255)
                                                                                                                    cGGGTTACGCCTGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                  <  2:980923/150‑1 (MQ=255)
                                                                                                                    cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                  <  1:1102692/150‑1 (MQ=255)
                                                                                                                     gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATTAGTCGGCACCTGGGAAATCTTATCg                                 <  2:1300576/150‑1 (MQ=255)
                                                                                                                     gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCg                                 <  1:221406/150‑1 (MQ=255)
                                                                                                                           cGCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCGCGCGAGAAATCATTACGGCAGGGCTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGGCATGAGTCGGCCCCGGGGAAATCTTTTCGGCAACg                           >  1:1262305/1‑150 (MQ=255)
                                                                                                                                   gagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACATTAGCGGAAAGGATAACTACCGGTATTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATAGGCAACGTCCATGcc                   >  1:68182/1‑150 (MQ=255)
                                                                                                                                    atttctcgcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCt                  >  2:1064663/8‑150 (MQ=255)
                                                                                                                                      cAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCttt                >  2:1259365/1‑150 (MQ=255)
                                                                                                                                       aGAATCGCGGGCAAGATTTCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               <  2:905315/150‑1 (MQ=255)
                                                                                                                                       aGAAGCGCGGGCAAGAGTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               <  1:1344244/150‑1 (MQ=255)
                                                                                                                                        gAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTca              <  2:399166/150‑1 (MQ=255)
                                                                                                                                           gcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGATTTGCCGGCACATTCTGGTACGGCCCAACGGCAGGAGTCGGCACCTGGGAAATGTTATCGGCAACTTCAATGCCTTTcccca           >  2:1340839/1‑150 (MQ=255)
                                                                                                                                            cgcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcaccac          <  2:1772453/150‑1 (MQ=255)
                                                                                                                                             gcgGGCATGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt         <  2:1716286/150‑1 (MQ=255)
                                                                                                                                             gcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt         <  2:524017/150‑1 (MQ=255)
                                                                                                                                               cgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACtt        >  1:996277/1‑150 (MQ=255)
                                                                                                                                               cgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACATGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACtt        >  1:462120/1‑150 (MQ=255)
                                                                                                                                                gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:1121146/1‑150 (MQ=255)
                                                                                                                                                gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:1396995/1‑150 (MQ=255)
                                                                                                                                                gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:159984/1‑150 (MQ=255)
                                                                                                                                                gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGAGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:1724848/1‑150 (MQ=255)
                                                                                                                                                gggCAAGATTGCACGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGCATAACTACCGGTTTTGACGGCACATTCTGGCACTGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       <  1:1164349/150‑1 (MQ=255)
                                                                                                                                                   cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc    <  2:1567636/150‑1 (MQ=255)
                                                                                                                                                   cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc    <  2:907583/150‑1 (MQ=255)
                                                                                                                                                    aaGATTGCCCGCGCGATAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTAATTGTTTTGACAGTACATTCTGGTACGGACCAAAGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCAAGCCTTTCCCCACTAAACCa   <  2:290734/150‑1 (MQ=255)
                                                                                                                                                    aaGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCa   <  1:219508/150‑1 (MQ=255)
                                                                                                                                                     aGATTGCCCGCGCGAGAAATCATTACTGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTTGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCaa  <  2:110047/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491555‑3491852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: