Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,705 A→C 6.7% intergenic (‑85/+20) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7050AC 6.7% 255.5 / 6.6 94intergenic (‑85/+20)yhfG/ppiAputative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base A (24/62);  new base C (4/3);  total (28/66)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

TGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491556‑3491852
                                                                                                                                                      |                                                                                                                                                   
tGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGa                                                                                                                                                     >  1:110047/1‑150 (MQ=255)
 gAAAATTACGCTTACGCGGTAGCTCCGAGAGAGGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAt                                                                                                                                                    <  1:269792/150‑1 (MQ=255)
 gAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCTGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAAGAt                                                                                                                                                    >  1:905315/1‑150 (MQ=255)
   aaaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTg                                                                                                                                                  <  1:2899/150‑1 (MQ=255)
   aaaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTg                                                                                                                                                  <  2:216113/150‑1 (MQ=255)
    aaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGc                                                                                                                                                 >  2:221406/1‑150 (MQ=255)
      ttacgattacgCTGAAGCTCCCAGAGACGGAACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGccc                                                                                                                                               <  2:1623213/150‑1 (MQ=255)
       tacgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcg                                                                                                                                              >  1:314579/1‑150 (MQ=255)
         cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCCGGGAAAAATTcccccc                                                                                                                                             >  2:1660665/1‑148 (MQ=255)
         cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcg                                                                                                                                            <  1:378250/150‑1 (MQ=255)
         cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTACACTTGATGCCTCCCTGAACGTCTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcg                                                                                                                                            >  1:1300576/1‑150 (MQ=255)
         cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGATTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTTCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcg                                                                                                                                            <  2:1104574/150‑1 (MQ=255)
           attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCTCGGGCAATCTTGCCcgcgct                                                                                                                                          <  1:1037094/150‑2 (MQ=255)
           attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcgcg                                                                                                                                          <  2:228941/150‑1 (MQ=255)
             tacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCgag                                                                                                                                        <  2:429793/150‑1 (MQ=255)
             accgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCgag                                                                                                                                        <  1:1776276/148‑1 (MQ=255)
              acgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGGGAGTGGCTTCACTGGATGCCTCCCTTACCGTGCCCATTTCCCCCAATTATAAGCGGCAAATCCGGGTAACGCCGGGAGAGCATAAGCGCGGGCAAGATTGCCCGCGCgaga                                                                                                                                       <  1:1703665/150‑1 (MQ=255)
                  tGAAGCTCCCAGACCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTCCCACAAGTATAATCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATc                                                                                                                                   <  1:496036/150‑1 (MQ=255)
                  ccaaGCTCCCAGAGACGGGACTTTTGCAAATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATATGCCCCAAGAATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATc                                                                                                                                   <  1:1898870/148‑1 (MQ=255)
                   gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCa                                                                                                                                  <  2:20444/150‑1 (MQ=255)
                   gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCa                                                                                                                                  <  1:1024200/150‑1 (MQ=255)
                   gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATACGGGTTACGCGGGGAGATCAGAAGCGCGTTCAAGCTTGCCCGCGCGATAACTCa                                                                                                                                  >  2:278604/1‑150 (MQ=255)
                    atgcgcccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGACCATTTGCCACAATTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAt                                                                                                                                 <  1:922388/145‑1 (MQ=255)
                    aaGCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTTATGCCTCCCTGAACGTGCCCATTTGCCACAAGAATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAt                                                                                                                                 <  1:1592048/150‑1 (MQ=255)
                     aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAtt                                                                                                                                <  2:529839/150‑1 (MQ=255)
                     aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTGACGCCGGGAGAGCAGAAGCGCGGGCAAGACTGCCCGCGCGAGAAATCAtt                                                                                                                                <  1:1540235/150‑1 (MQ=255)
                            agagACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAAATTTCCCGCGCGAGAAATAATTACGGCAg                                                                                                                         >  1:12542/1‑150 (MQ=255)
                                   ggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCGAGATTTACCGCGGCAGCAATCATTTCGGCgggccgttt                                                                                                                  >  1:907583/1‑144 (MQ=255)
                                    gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg                                                                                                                 <  1:1847590/150‑1 (MQ=255)
                                    gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg                                                                                                                 <  1:473583/150‑1 (MQ=255)
                                       ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                              <  2:1647129/150‑1 (MQ=255)
                                           gCTTATCGGTGTGTTTATACTCTTGATGCCTCCCTGAACGTTTCCATTTGCCACATCTCTAAGCGGCTAATCCGGGTTACGCAGGGAGAGCAGAAGCGCGGGCAAGTTTGCCCGCGCGAGAAATCATTACGGCAGTACTTTAGCGGAAAg                                                                                                          <  2:1388411/150‑1 (MQ=255)
                                           gCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAATCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAg                                                                                                          <  2:1550876/150‑1 (MQ=255)
                                           gCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAg                                                                                                          <  1:1524231/150‑1 (MQ=255)
                                            cTTATCGGTGAGGTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGACGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAgg                                                                                                         <  1:227973/150‑1 (MQ=255)
                                             ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                        <  2:1579494/150‑1 (MQ=255)
                                             ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTACCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                        >  2:1368098/1‑150 (MQ=255)
                                              tATCGGTGAGTTTCTTCACTTGCTGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGCTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCCCGAGAAATCATTACCGCCGGACTTTTGCGGAAAGGaa                                                                                                       >  2:991956/1‑149 (MQ=255)
                                              tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCCGGCAAGCATGGCCGCCCGGCAAATAATTAATGGCGGCCTTTAGGGGAAAgggt                                                                                                       >  1:1867058/1‑148 (MQ=255)
                                                 gggTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAc                                                                                                    <  2:1601288/149‑1 (MQ=255)
                                                 cGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAc                                                                                                    <  1:605403/150‑1 (MQ=255)
                                                 cGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAc                                                                                                    <  2:1342982/150‑1 (MQ=255)
                                                     gAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGGGAGAAATCATTACGGCAGGACGTTTGCGGAAAGGATAACGAcc                                                                                                >  2:94801/1‑150 (MQ=255)
                                                      aGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCg                                                                                               <  2:486727/150‑1 (MQ=255)
                                                       gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATATGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGCAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAATACGGCAGGACTTTAGCGGAAAGGATAACTGCCgg                                                                                              >  1:1222643/1‑150 (MQ=255)
                                                         ttcttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGtt                                                                                            <  1:1076601/150‑1 (MQ=255)
                                                           cttcACTTGATGCCGCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGtttt                                                                                          <  1:602319/150‑1 (MQ=255)
                                                              cACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAATACGGCAGGACTTTAGCGGAAAGGAAAACTACCGGTTTTGAc                                                                                       >  2:14398/1‑150 (MQ=255)
                                                                  tGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                   <  2:314579/150‑1 (MQ=255)
                                                                  tGAAGCCTCCCTTAACGTGCCCATTTGCCACAAGTATCAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                   <  2:1143318/150‑1 (MQ=255)
                                                                          cccTGAACGTGTCCTTGTGCCTCAAGTCTAAGCGGCAAATCCTGGTTACGCCGGGAGAGCAGAAGCGCTGGCAAGATTGCCCGCGCGAGAATTCATTAAGGCAGGACTTTAGCGTAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  1:1176467/150‑1 (MQ=255)
                                                                          cccTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCTGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  1:1175320/150‑1 (MQ=255)
                                                                          cccTGAACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  2:877065/150‑1 (MQ=255)
                                                                          cccTGAACGGGGCCATTTGCCACCAGTATAAGCGGCAAATCCGGGCTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGACAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                           <  1:519440/150‑1 (MQ=255)
                                                                              caaCGTGTCCAATTGCCACAAGTAAAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACg                                                                       <  2:1670556/149‑1 (MQ=255)
                                                                                aCGTGTCCATTTGCCACAAGTATAAGCGGCAACTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGa                                                                     <  2:86706/150‑1 (MQ=255)
                                                                                   tgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTGACGCGCGACAAAAAAATTATGCAGGTCATTATGGGAGACGATAACAACCCGGTTTGACGGCCCCTTGAGGTACgggacc                                                                  >  2:924721/1‑146 (MQ=255)
                                                                                   tgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCa                                                                  >  1:1359855/1‑150 (MQ=255)
                                                                                          ttGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCAt                                                           <  2:845286/150‑1 (MQ=255)
                                                                                            gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTTCCCGCGCGAGAAAACATTACGGCAGGTCATTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGa                                                         >  1:1258614/1‑150 (MQ=255)
                                                                                             ccACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAAGTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAg                                                        <  2:104893/150‑1 (MQ=255)
                                                                                              cacaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGGTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGt                                                       <  2:389697/150‑1 (MQ=255)
                                                                                                 caGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTGTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCgg                                                    <  1:1406631/149‑1 (MQ=255)
                                                                                                  aGTATAAGCGTCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGTGCAAGATTGCCCGCGCGAGAAATCATTACGTCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGc                                                   <  2:1338989/150‑1 (MQ=255)
                                                                                                       aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg                                              <  2:220315/150‑1 (MQ=255)
                                                                                                              aaaTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGATAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATc                                       <  2:1005198/150‑1 (MQ=255)
                                                                                                               aaTCCGGGTTACGCCGGGAGCGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCt                                      <  1:377545/150‑1 (MQ=255)
                                                                                                               aaTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCt                                      <  2:1106262/150‑1 (MQ=255)
                                                                                                                   cGGGTTACGCCTGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                  <  2:980923/150‑1 (MQ=255)
                                                                                                                   cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                  <  1:1102692/150‑1 (MQ=255)
                                                                                                                    gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATTAGTCGGCACCTGGGAAATCTTATCg                                 <  2:1300576/150‑1 (MQ=255)
                                                                                                                    gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCg                                 <  1:221406/150‑1 (MQ=255)
                                                                                                                          cGCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCGCGCGAGAAATCATTACGGCAGGGCTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGGCATGAGTCGGCCCCGGGGAAATCTTTTCGGCAACg                           >  1:1262305/1‑150 (MQ=255)
                                                                                                                                  gagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACATTAGCGGAAAGGATAACTACCGGTATTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATAGGCAACGTCCATGcc                   >  1:68182/1‑150 (MQ=255)
                                                                                                                                   atttctcgcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCt                  >  2:1064663/8‑150 (MQ=255)
                                                                                                                                     cAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCttt                >  2:1259365/1‑150 (MQ=255)
                                                                                                                                      aGAATCGCGGGCAAGATTTCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               <  2:905315/150‑1 (MQ=255)
                                                                                                                                      aGAAGCGCGGGCAAGAGTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               <  1:1344244/150‑1 (MQ=255)
                                                                                                                                       gAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTca              <  2:399166/150‑1 (MQ=255)
                                                                                                                                          gcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGATTTGCCGGCACATTCTGGTACGGCCCAACGGCAGGAGTCGGCACCTGGGAAATGTTATCGGCAACTTCAATGCCTTTcccca           >  2:1340839/1‑150 (MQ=255)
                                                                                                                                           cgcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcaccac          <  2:1772453/150‑1 (MQ=255)
                                                                                                                                            gcgGGCATGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt         <  2:1716286/150‑1 (MQ=255)
                                                                                                                                            gcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt         <  2:524017/150‑1 (MQ=255)
                                                                                                                                              cgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACtt        >  1:996277/1‑150 (MQ=255)
                                                                                                                                              cgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACATGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACtt        >  1:462120/1‑150 (MQ=255)
                                                                                                                                               gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:1121146/1‑150 (MQ=255)
                                                                                                                                               gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:1396995/1‑150 (MQ=255)
                                                                                                                                               gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:159984/1‑150 (MQ=255)
                                                                                                                                               gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGAGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:1724848/1‑150 (MQ=255)
                                                                                                                                               gggCAAGATTGCACGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGCATAACTACCGGTTTTGACGGCACATTCTGGCACTGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       <  1:1164349/150‑1 (MQ=255)
                                                                                                                                                  cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc    <  2:1567636/150‑1 (MQ=255)
                                                                                                                                                  cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc    <  2:907583/150‑1 (MQ=255)
                                                                                                                                                   aaGATTGCCCGCGCGATAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTAATTGTTTTGACAGTACATTCTGGTACGGACCAAAGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCAAGCCTTTCCCCACTAAACCa   <  2:290734/150‑1 (MQ=255)
                                                                                                                                                   aaGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCa   <  1:219508/150‑1 (MQ=255)
                                                                                                                                                    aGATTGCCCGCGCGAGAAATCATTACTGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTTGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCaa  <  2:110047/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491556‑3491852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: