Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,529,746 A→T 5.4% E133D (GAA→GAT hslR → ribosome‑associated heat shock protein Hsp15

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,529,7460AT 5.4% 356.8 / 5.7 120E133D (GAA→GAThslRribosome‑associated heat shock protein Hsp15
Reads supporting (aligned to +/- strand):  ref base A (67/46);  new base T (5/2);  total (72/48)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 122 total aligned reads displayed.

TATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGT  >  NC_000913/3529600‑3529895
                                                                                                                                                  |                                                                                                                                                     
  tGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGCCGCCAGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACTGTGATAAACt                                                                                                                                                  >  2:926200/1‑150 (MQ=255)
   gAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTg                                                                                                                                                 <  1:1746350/150‑1 (MQ=255)
     agagACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTc                                                                                                                                               <  1:834404/150‑1 (MQ=255)
     agagACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTc                                                                                                                                               >  1:1655391/1‑150 (MQ=255)
      gagaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTcc                                                                                                                                              >  2:672778/1‑149 (MQ=255)
       agaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTcgc                                                                                                                                             <  1:247293/150‑3 (MQ=255)
       agaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCAc                                                                                                                                             >  2:1442094/1‑150 (MQ=255)
        gaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTACCCATGCCGCACCCGGACCGACGCCCGGACAAAACAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCAcc                                                                                                                                            <  1:196486/150‑1 (MQ=255)
        gaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCAcc                                                                                                                                            >  2:203321/1‑150 (MQ=255)
        gaCTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCAcc                                                                                                                                            <  1:879467/150‑1 (MQ=255)
         aCTGTGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTACCCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTTATTCACTGTCGccg                                                                                                                                           <  1:47447/150‑2 (MQ=255)
              ggAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAag                                                                                                                                      >  1:1218921/1‑150 (MQ=255)
              ggAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAag                                                                                                                                      >  2:1660858/1‑150 (MQ=255)
                 aactgtAGAGAAACGAGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAagaga                                                                                                                                   <  2:798468/147‑1 (MQ=255)
                    tgtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgat                                                                                                                                >  1:461748/1‑150 (MQ=255)
                    tgtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgat                                                                                                                                >  1:1597821/1‑150 (MQ=255)
                     gtAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAgatg                                                                                                                               >  2:808250/1‑150 (MQ=255)
                      tAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAAATTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGTATAACTGTCACCTGCAAGAGAgatga                                                                                                                              <  1:526504/150‑1 (MQ=255)
                        gagaAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGAtt                                                                                                                            <  1:31207/150‑1 (MQ=255)
                           aaaCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATg                                                                                                                         >  2:1024672/1‑150 (MQ=255)
                             aCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGCGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGcc                                                                                                                       >  1:1018509/1‑150 (MQ=255)
                             aCGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGACGCACCCGGCCCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGcc                                                                                                                       <  1:561531/150‑1 (MQ=255)
                               gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGCCGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAACCACGGCGACAGTGAATAACTGTCCCCTGCAAGAGAGATGATTATGCCGc                                                                                                                     >  2:1765496/1‑150 (MQ=255)
                               gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGc                                                                                                                     >  1:1532198/1‑150 (MQ=255)
                               gcgAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGc                                                                                                                     >  1:1417565/1‑150 (MQ=255)
                                        tGGCGCTGTCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAAATGTCACCTGCAAGAGAGATTATTATGCCGCAACATGAcc                                                                                                            <  2:546134/150‑1 (MQ=255)
                                        tGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGAcc                                                                                                            <  1:1070112/150‑1 (MQ=255)
                                            gcTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGAGTTAACCCCGGCGACAGTGAATAACTGTCACCTGCACGAGAGATGATTATGCCGCAACATGACCAAtt                                                                                                        >  1:1176728/1‑150 (MQ=255)
                                             cTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTa                                                                                                       <  1:1613983/150‑1 (MQ=255)
                                                 cACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATc                                                                                                   >  1:670356/1‑150 (MQ=255)
                                                 cACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATc                                                                                                   >  1:432658/1‑150 (MQ=255)
                                                         cTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTg                                                                                           <  1:1082192/150‑1 (MQ=255)
                                                           tAATGCCTTAACCATGCCGCACCCGTACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAATCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCTCAACATGACCAATTACATCGCTATCTGtt                                                                                         <  1:92486/150‑1 (MQ=255)
                                                           tAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGtt                                                                                         <  2:1500748/150‑1 (MQ=255)
                                                             aTGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTg                                                                                       <  2:915698/150‑1 (MQ=255)
                                                              tGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGa                                                                                      >  2:1074058/1‑150 (MQ=255)
                                                               gCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGCCAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGaa                                                                                     >  1:475022/1‑150 (MQ=255)
                                                                ccTTAACCATGCCGCACCCGGACCGGCGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGaaa                                                                                    >  2:796670/1‑150 (MQ=255)
                                                                ccTTAACCATGCCGCACCCGGACCGACGCCGGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGaaa                                                                                    <  2:1552562/150‑1 (MQ=255)
                                                                   tAACCATGCCGCACCAGGACAGACGCGCGGACAAAAAAGAGCGCCGCGACCTGTTAAGATTTAAACAAGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACt                                                                                 <  1:336471/150‑1 (MQ=255)
                                                                    aaCCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACtt                                                                                <  2:1361158/150‑1 (MQ=255)
                                                                     aCCCTGCCGCACCCGGCCCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAAGTACATCGCTATCTGGTTGAAAACttt                                                                               >  2:297526/1‑150 (MQ=255)
                                                                     aCCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACttt                                                                               >  1:1851420/1‑150 (MQ=255)
                                                                      ccttGCCGAACCCGGACCGACGCCCGGACAAAAATGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACATGCAAGAGAGATGATTATGCAGCTAAATGACCAATTACATCGCTATCTGTTTGAAAACTTTg                                                                              <  1:1004665/147‑1 (MQ=255)
                                                                      ccATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGAGAGTGAATAACGGTCACCTGCAAGCGAGATGATTATGCCGCAACATGACCAATTACTTAGCTTTCTGTTTGAAATCtttc                                                                              >  1:1260723/1‑149 (MQ=255)
                                                                      ccATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTg                                                                              >  1:1887214/1‑150 (MQ=255)
                                                                            cGCACCCCGACCGACGCCCGGACAAAAAACAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTgc                                                                        <  2:168909/150‑1 (MQ=255)
                                                                                cccGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAGCTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcgg                                                                    >  1:1214828/1‑150 (MQ=255)
                                                                                cccGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCgcgg                                                                    >  1:1056085/1‑150 (MQ=255)
                                                                                  ctgACCGACGCCCGGACAAAACAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTATGCCGTGCgcggcg                                                                  <  2:1011251/148‑1 (MQ=255)
                                                                                      ccGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACt                                                              <  1:765868/150‑1 (MQ=255)
                                                                                        gACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTgg                                                            <  2:620070/150‑1 (MQ=255)
                                                                                          cGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTa                                                          >  2:1451833/1‑150 (MQ=255)
                                                                                            cccGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAAc                                                        >  2:50263/1‑150 (MQ=255)
                                                                                            cccGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAAc                                                        <  2:1025077/150‑1 (MQ=255)
                                                                                                gACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGCCCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGtt                                                    <  1:501618/150‑1 (MQ=255)
                                                                                                gACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTCCAAGAGAGCTGATTATGCCGCATCATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTACCCGtt                                                    >  1:293445/1‑150 (MQ=255)
                                                                                                 aCAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGttt                                                   >  2:1823332/1‑150 (MQ=255)
                                                                                                    aaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCgg                                                <  1:547627/150‑1 (MQ=255)
                                                                                                    aaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCgg                                                <  1:352143/150‑1 (MQ=255)
                                                                                                    aaaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTACgg                                                >  1:1758064/1‑150 (MQ=255)
                                                                                                     aaaaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGa                                               >  2:7084/1‑150 (MQ=255)
                                                                                                       aaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGATTAACTGTCACCTGCAAGAGAGATGATTATGCCGCAAAATGACCATTTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAAGTGGTAACCGTTTCGGata                                             >  1:789284/1‑148 (MQ=255)
                                                                                                       aaGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATCCCGCAACATGACCAAGTACAGCGCTATCTGTTTGAAAAATTGGCCGTGCTCTGAGAACTGGTAACCGGTTCGGaaa                                             >  1:789285/1‑150 (MQ=255)
                                                                                                        agagCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAAc                                            >  2:1755132/1‑150 (MQ=255)
                                                                                                        agagCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAAc                                            <  2:1331550/150‑1 (MQ=255)
                                                                                                            cgccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTg                                        <  2:1655391/150‑1 (MQ=255)
                                                                                                             gccgcGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGc                                       <  1:570343/150‑1 (MQ=255)
                                                                                                                    ccTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGAt                                >  1:1386344/1‑150 (MQ=255)
                                                                                                                     cTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATc                               <  2:1853965/150‑1 (MQ=255)
                                                                                                                       gTTACGATTTAAACACGGCGACTGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCt                             <  2:1270616/150‑1 (MQ=255)
                                                                                                                       gTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCt                             >  1:1629654/1‑150 (MQ=255)
                                                                                                                       gTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCATGCAACAGATCCt                             >  2:411610/1‑150 (MQ=255)
                                                                                                                         tACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTg                           <  2:1090474/150‑1 (MQ=255)
                                                                                                                         tACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTg                           <  2:1719052/150‑1 (MQ=255)
                                                                                                                         tACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTg                           <  2:1849949/150‑1 (MQ=255)
                                                                                                                          aCGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTTTCTGTTTGAAGACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTga                          >  2:1191179/1‑150 (MQ=255)
                                                                                                                           cGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCACCATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAACCCCTGCAACAGATCCTTgag                         >  1:112855/1‑150 (MQ=255)
                                                                                                                           cGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgag                         <  2:445874/150‑1 (MQ=255)
                                                                                                                            gATTTAAACACGGCGTCATTGTATAACTGTCACCTGCAAGAGAGATGCTTTTGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGTAAACCCTGCAACAGATCCTTgaga                        <  1:926200/150‑1 (MQ=255)
                                                                                                                            gATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgaga                        >  1:316822/1‑150 (MQ=255)
                                                                                                                            gATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTATGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTgaga                        >  2:764944/1‑150 (MQ=255)
                                                                                                                              tttAAACACGGCGACAGTGAATATCTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAAc                      <  1:1653878/150‑1 (MQ=255)
                                                                                                                               ttAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAccc                     >  1:1098790/1‑150 (MQ=255)
                                                                                                                                tAACCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTAAATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAACCCCTGCAACAGATCCTTGAGAACCa                    >  2:906314/1‑150 (MQ=255)
                                                                                                                                tAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCa                    >  2:477208/1‑150 (MQ=255)
                                                                                                                                tAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATAATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCATGCAACATATCCTTGAGAAcaa                    >  2:920432/1‑148 (MQ=255)
                                                                                                                                 aaaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCAc                   <  1:1442094/150‑1 (MQ=255)
                                                                                                                                  aaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACg                  <  2:1218921/150‑1 (MQ=255)
                                                                                                                                  aaCACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACg                  >  1:251515/1‑150 (MQ=255)
                                                                                                                                   tgcaggtgACAGTTATTCACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGa                 >  1:1809870/8‑150 (MQ=255)
                                                                                                                                       ggtgACAGTTATTCACTGTCGCCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTAt             >  1:1517370/4‑150 (MQ=255)
                                                                                                                                        gCGACAGTGAATAACTGTCACCTGGAAGAGAGATGATTATGCCGCAACATGACCAAATACAACGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAAGTGGTAACCGTTTCGGAAACCCTGCAGCAGATCCTTGGGCACCACGATTaaa            >  1:332376/1‑148 (MQ=255)
                                                                                                                                           aCAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGc         >  2:284272/1‑150 (MQ=255)
                                                                                                                                            cAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCa        >  2:1901692/1‑150 (MQ=255)
                                                                                                                                             aGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAg       >  2:87440/1‑150 (MQ=255)
                                                                                                                                              gTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGCTTATCCGCAGc      >  1:691757/1‑150 (MQ=255)
                                                                                                                                              gTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATAGCTATCTGTTTGAAAACTTTGACGTGCGCGGCGAACTGGTAACCGTTTAGGAAACCCGGCAACAGATCCTTGAGAACATCGATTATCCGCAGc      >  1:904335/1‑150 (MQ=255)
                                                                                                                                                  aTAACTGTCACCTGCAAGATAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGt  <  2:502401/150‑1 (MQ=255)
                                                                                                                                                  aTAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGt  >  1:1726876/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TATGAAGAGACTGCGGAAAGTGTAGAGAAACGCGAAAAAATGGCGCTGGCACGTAAACTTAATGCCTTAACCATGCCGCACCCGGACCGACGCCCGGACAAAAAAGAGCGCCGCGACCTGTTACGATTTAAACACGGCGACAGTGAATAACTGTCACCTGCAAGAGAGATGATTATGCCGCAACATGACCAATTACATCGCTATCTGTTTGAAAACTTTGCCGTGCGCGGCGAACTGGTAACCGTTTCGGAAACCCTGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGT  >  NC_000913/3529600‑3529895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: