Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,304 A→G 5.3% intergenic (‑39/+12) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,3040AG 5.3% 404.3 / 8.9 134intergenic (‑39/+12)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (65/61);  new base G (4/3);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 135 total aligned reads displayed.

CCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGG  >  NC_000913/3918157‑3918452
                                                                                                                                                   |                                                                                                                                                    
ccTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgc                                                                                                                                                    >  1:1197661/1‑150 (MQ=255)
   gCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccgc                                                                                                                                                 >  1:1882898/1‑150 (MQ=255)
      aTTTACGCATTTTGGAAGCGGCGACCATCACCATCGCTTTAGTGATCTTTTGCGTGTTCTGGCCGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTGGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                                                                              <  1:29643/150‑1 (MQ=255)
       tttACGCATTTTGGACGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTCCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                                                                             <  1:801579/150‑2 (MQ=255)
       tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTGTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGCGCTTCTCCGCAATGCCTTGCGGCCTGCCCTACGGCAAGCCGCCAGa                                                                                                                                             >  1:927476/1‑150 (MQ=255)
         tACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                                                                           >  2:1249759/1‑150 (MQ=255)
         tACGCATGTTGGAAGCTGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                                                                           <  1:882806/150‑1 (MQ=255)
          aCGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGCCGt                                                                                                                                          >  2:826771/1‑150 (MQ=255)
           cGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGTGGCCTGCCCTAAGGCAAGCCGCCAGACGtt                                                                                                                                         >  2:1439212/1‑150 (MQ=255)
           cGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGCCGtt                                                                                                                                         >  2:936427/1‑150 (MQ=255)
              aTTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                                                                      <  2:1798700/150‑1 (MQ=255)
              aTTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                                                                      >  1:1524647/1‑150 (MQ=255)
                    gAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGAtt                                                                                                                                >  1:1150555/1‑150 (MQ=255)
                     aaGGGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTg                                                                                                                               <  2:1441684/150‑1 (MQ=255)
                     aaGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTg                                                                                                                               <  1:960805/150‑1 (MQ=255)
                      aGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTgg                                                                                                                              >  2:2581/1‑150 (MQ=255)
                      aGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTgg                                                                                                                              >  1:8981/1‑150 (MQ=255)
                        cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                                                                            <  1:1794727/150‑1 (MQ=255)
                        cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                                                                            <  2:719803/150‑1 (MQ=255)
                        cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCACGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                                                                            <  2:1392717/150‑1 (MQ=255)
                         ggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                                           >  1:1083843/1‑150 (MQ=255)
                           cgACCATCTCCCTCGCTTTAGTGATCTTTTGCGGGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTACGGAAAGCCGCCAGCCTTTACCAGGAGTGGGTTgg                                                                                                                         >  2:315586/1‑149 (MQ=255)
                           cgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGc                                                                                                                         >  1:1229372/1‑150 (MQ=255)
                              ccATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCttt                                                                                                                      >  2:1385474/1‑150 (MQ=255)
                              ccATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCttt                                                                                                                      >  2:726844/1‑150 (MQ=255)
                                aTCTCCATCGCTTTAGTGATCTTTTGCTTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGa                                                                                                                    <  2:1704373/150‑1 (MQ=255)
                                  ctcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                                  <  1:142914/150‑1 (MQ=255)
                                   tcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGAATGGGTTGCTTTGAAgg                                                                                                                 >  1:980890/1‑150 (MQ=255)
                                   tcCATCGCTTTAGTGATCTTTTGCGTGTTATGGACGCTTGGGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                                                                                 <  2:958376/150‑1 (MQ=255)
                                     cATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGaa                                                                                                               >  2:1690856/1‑150 (MQ=255)
                                     cATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGaa                                                                                                               >  2:1792201/1‑150 (MQ=255)
                                      aTCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGCCCTTAACAGGATTGGGGTGGTTTGAAGGaaa                                                                                                              >  2:1733041/1‑149 (MQ=255)
                                       tCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATc                                                                                                             >  2:1822671/1‑150 (MQ=255)
                                            ttAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGa                                                                                                        <  2:1612963/150‑1 (MQ=255)
                                              aGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATg                                                                                                      <  1:1834503/150‑1 (MQ=255)
                                               gTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGc                                                                                                     >  1:812827/1‑150 (MQ=255)
                                                    cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTc                                                                                                <  2:647756/150‑1 (MQ=255)
                                                     tttgGCGTGCTCTGGACGCTGGCGATCTTACTACGAATCTCTTTTCCGCCGGCCATGAGCTGCTCCTCAATGCCTTGCGGCCGGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCa                                                                                               <  2:294673/150‑1 (MQ=255)
                                                       ttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGc                                                                                             >  2:818497/1‑150 (MQ=255)
                                                        tGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCt                                                                                            >  1:1342422/1‑150 (MQ=255)
                                                          cGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTg                                                                                          <  1:1608754/150‑1 (MQ=255)
                                                          cGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTg                                                                                          <  1:812373/150‑1 (MQ=255)
                                                               taatgtcGCTTGCGATCTTTCTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGATTCGAGGATGCCTTTCAGCTTGCCTTc                                                                                     <  2:949067/144‑1 (MQ=255)
                                                               tCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTc                                                                                     <  1:1762669/150‑1 (MQ=255)
                                                                cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCg                                                                                    <  2:49264/150‑1 (MQ=255)
                                                                  ggccGCTTGCGATCTTACTACGTAGCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTTAGGCAAGCCGCCAGACGTTACGAGGATTGGGTTGCTTTGAAGTAATCGATGATGCCTTTCAGCTTGCCTTCGAt                                                                                  <  1:1097662/147‑1 (MQ=255)
                                                                   gACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAtt                                                                                 <  2:570813/150‑1 (MQ=255)
                                                                    aCGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTTCGGCCTGCCCAAAGGCAATACGCGAGTCGTTACAAGGAATGGGTAGCTTTGAAGGAATCGAGGATGCCTATCAGCTTGCCTTCGAttt                                                                                >  1:903281/1‑150 (MQ=255)
                                                                      gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                                                                              <  2:814318/150‑1 (MQ=255)
                                                                      gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                                                                              <  2:1112663/150‑1 (MQ=255)
                                                                      gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                                                                              <  1:409530/150‑1 (MQ=255)
                                                                      gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                                                                              <  1:1541495/150‑1 (MQ=255)
                                                                      gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTGCTCCTCAATGCCTTGCGTCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                                                                              <  1:1721159/150‑1 (MQ=255)
                                                                        ttGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtc                                                                            >  2:611531/1‑150 (MQ=255)
                                                                         tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                           <  2:1054197/150‑1 (MQ=255)
                                                                         tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                           <  1:41017/150‑1 (MQ=255)
                                                                         tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTCGAAGGAAGCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                           >  2:1007077/1‑150 (MQ=255)
                                                                           cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGtt                                                                         >  2:708021/1‑150 (MQ=255)
                                                                             aTCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGt                                                                       <  1:54512/150‑1 (MQ=255)
                                                                              tCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa                                                                      <  2:1066098/150‑1 (MQ=255)
                                                                               cTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg                                                                     <  2:847909/150‑1 (MQ=255)
                                                                                ttACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCGGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCCGCTTGGCTTCGATTTCGTCGTTGTGGc                                                                    >  2:1774798/1‑150 (MQ=255)
                                                                                ttACTACGTATCACTTTTGCGCCGGCCATGAGCTTCCCCTCAATGCCTTGCGGCCTGGCCTAAGGCAAGCCGCGAGACGTTACCAGGATTGGGTGGCTTTGAAGGAATCGAGGATTCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGc                                                                    <  1:1435593/150‑1 (MQ=255)
                                                                                      cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCgg                                                              <  1:1343025/150‑1 (MQ=255)
                                                                                         aTCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGGTTGCCTTCGATTTCGTCGGTGTAGCCACCGGTcg                                                           >  2:1751461/1‑149 (MQ=255)
                                                                                           ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                         >  2:1211701/1‑150 (MQ=255)
                                                                                           ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                         >  1:834897/1‑150 (MQ=255)
                                                                                           ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                         >  1:1301093/1‑150 (MQ=255)
                                                                                            tctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt                                                        <  1:216115/150‑1 (MQ=255)
                                                                                            tctTGTGCGCCGGCCAGGTGCTTCTCCTCACTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGGTACCCGGCTTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCGTCGATTTCGTCGTTGTAGCCACCGGTCTGGt                                                        <  1:1334293/150‑1 (MQ=255)
                                                                                             ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGATGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                                       >  1:1057371/1‑150 (MQ=255)
                                                                                              ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTg                                                      <  1:33375/150‑1 (MQ=255)
                                                                                              ttttGAGCCGTCCATGAGCTTCTCCTCAATTCCTTGCGGCCTTCACTAAGGCATGCCGCCAGTCGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGGTTGCCTTCGATTTCGTCGTTGTAGCCACGGGTCTGGTTg                                                      <  2:1361998/150‑1 (MQ=255)
                                                                                                    gcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATctct                                                >  2:1739062/1‑150 (MQ=255)
                                                                                                           aTGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTCGCCACCGGGCTGGTTGAGCTCTTgcagca                                         <  2:1168257/150‑1 (MQ=255)
                                                                                                           aTGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCa                                         >  2:1887292/1‑150 (MQ=255)
                                                                                                            tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCaa                                        >  2:1830438/1‑150 (MQ=255)
                                                                                                             gAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAAc                                       <  1:1455208/150‑1 (MQ=255)
                                                                                                              aGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACg                                      <  2:908686/150‑1 (MQ=255)
                                                                                                                    tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCAGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGt                                <  1:1403006/150‑1 (MQ=255)
                                                                                                                     cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTg                               >  1:1347122/1‑150 (MQ=255)
                                                                                                                     cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTg                               >  1:170804/1‑150 (MQ=255)
                                                                                                                      ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCCTCAACGGAGCGTGa                              >  1:1712948/1‑150 (MQ=255)
                                                                                                                      ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa                              <  1:1822671/150‑1 (MQ=255)
                                                                                                                       tcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGAt                             <  2:458459/150‑1 (MQ=255)
                                                                                                                             ccTTGCGGCCTGCCCTACGGCAAGCCGCCAGACGTTACCAGGATTGTGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                       <  1:1476667/150‑1 (MQ=255)
                                                                                                                              ctggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTc                      >  1:739459/4‑150 (MQ=255)
                                                                                                                               ttGCGGCCTGCCCTAATGCTAGCCGCCAGACGTTACCTGGATTGGGTTGCTTTGAAGGTATAGAGTATGCCTTTCAGCTTGCATTCGATTTCGTCGTTGTAGCATCCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCg                     <  1:669665/150‑1 (MQ=255)
                                                                                                                               ttGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCg                     >  1:58839/1‑150 (MQ=255)
                                                                                                                               ttGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCg                     >  1:1251310/1‑150 (MQ=255)
                                                                                                                                ggCGGCTTGCCTTAGGGCAGGCCGCAAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCgc                    >  1:678378/2‑149 (MQ=255)
                                                                                                                                  cGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCATCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACg                  <  1:347896/150‑1 (MQ=255)
                                                                                                                                    gcttGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTa                >  2:208291/4‑150 (MQ=255)
                                                                                                                                       tGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGc             >  1:714182/1‑150 (MQ=255)
                                                                                                                                         cccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca           >  1:578115/1‑150 (MQ=255)
                                                                                                                                         cccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCGTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAGCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGccc           >  2:1814048/1‑149 (MQ=255)
                                                                                                                                           cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc         <  2:1342422/150‑1 (MQ=255)
                                                                                                                                               ggCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCagag     >  2:460172/1‑150 (MQ=255)
                                                                                                                                                 cAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCg   >  2:1129976/1‑150 (MQ=255)
                                                                                                                                                  aaGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCgg  <  1:1806813/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGG  >  NC_000913/3918157‑3918452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: