Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,926 A→T 5.7% intergenic (‑113/+86) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,9260AT 5.7% 376.9 / 7.3 132intergenic (‑113/+86)ppiC/yifNpeptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
Reads supporting (aligned to +/- strand):  ref base A (68/54);  new base T (4/4);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 135 total aligned reads displayed.

TACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAAC  >  NC_000913/3959778‑3960075
                                                                                                                                                    |                                                                                                                                                     
tACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAt                                                                                                                                                      >  2:600091/1‑150 (MQ=255)
 aCAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATc                                                                                                                                                     >  1:729396/1‑150 (MQ=255)
    agtatatGCAGGGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGATTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGTGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCgg                                                                                                                                                  <  1:1731672/147‑1 (MQ=255)
    aGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGACGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCgg                                                                                                                                                  <  1:1708692/150‑1 (MQ=255)
    aGGATATGCAGTGCTGCTGCTGGTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCGGAAAATCCggg                                                                                                                                                 <  2:830145/150‑1 (MQ=255)
          tGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCtttt                                                                                                                                            <  1:1233187/150‑3 (MQ=255)
           gCAGTTCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGTCAAAAGCCGGGAAAATCCGGGCTTCGc                                                                                                                                           <  2:585235/150‑1 (MQ=255)
           gCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGc                                                                                                                                           >  1:1399869/1‑150 (MQ=255)
            cAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTTGCTGGTACATATATGGCATAAGCCCGGATTTTCCGGGCttttgc                                                                                                                                          <  1:1321112/150‑5 (MQ=255)
              gTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAgg                                                                                                                                        <  2:1482243/150‑1 (MQ=255)
                gctgctgctGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTc                                                                                                                                      <  1:1402485/150‑1 (MQ=255)
                gctgctgctGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTc                                                                                                                                      <  1:1024064/150‑1 (MQ=255)
                gctgctgctGTTTTTGCCATGAACGTGCCTTGAGTGAATAAATGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGACAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGTAAAATCCGGGCTTCGCAGGTc                                                                                                                                      >  2:1624956/1‑150 (MQ=255)
                    ctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAGTCCGGGCTTCGCAGGTCCCTc                                                                                                                                  >  1:1454694/1‑150 (MQ=255)
                     tgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCa                                                                                                                                 >  2:1494915/1‑150 (MQ=255)
                      gctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCaa                                                                                                                                >  1:154888/1‑150 (MQ=255)
                        tgTTTTTTCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGAATGATCCGCAGATGCTAACGTAATTGTCAGTGAGGGAGTGGCGGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCACTCAAgg                                                                                                                              <  2:1438360/150‑1 (MQ=255)
                        tgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgg                                                                                                                              >  2:824912/1‑150 (MQ=255)
                          tttttGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgggg                                                                                                                            <  2:539736/150‑1 (MQ=255)
                           ttttGCCATGATCGTGCCTTGAGTTAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATCGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGga                                                                                                                           <  2:971813/150‑1 (MQ=255)
                                ccATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                      >  1:1577784/1‑150 (MQ=255)
                                ccATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                      >  2:419450/1‑150 (MQ=255)
                                ccATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                      >  2:422950/1‑150 (MQ=255)
                                ccATGATCGTGCCTTGAGCGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                      >  2:1429079/1‑150 (MQ=255)
                                      tCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAgg                                                                                                                <  2:73788/150‑1 (MQ=255)
                                      tCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATATTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCTAGGGGAGAAAACTTAgg                                                                                                                >  2:445772/1‑150 (MQ=255)
                                              tGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                                                                        >  2:735514/1‑150 (MQ=255)
                                                   gAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                                                                   >  1:1135329/1‑150 (MQ=255)
                                                      taatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                                                                <  1:1102301/150‑1 (MQ=255)
                                                         ttGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                                                                             <  1:1844972/150‑1 (MQ=255)
                                                         ttGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                                                                             <  1:1506769/150‑1 (MQ=255)
                                                          tGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                                                                            >  1:618425/1‑150 (MQ=255)
                                                          tGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                                                                            >  1:650298/1‑150 (MQ=255)
                                                           gTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                                                                           >  2:344405/1‑150 (MQ=255)
                                                           gTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                                                                           >  2:1817035/1‑150 (MQ=255)
                                                           gTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                                                                           >  2:1809644/1‑150 (MQ=255)
                                                           gTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                                                                           >  1:1487635/1‑150 (MQ=255)
                                                           gTCAGGAGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGta                                                                                           >  2:1436674/1‑149 (MQ=255)
                                                            tCAGGCGGCGAATTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                                                                          <  2:952441/150‑1 (MQ=255)
                                                             cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGGCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                                                                                         <  2:1076726/150‑1 (MQ=255)
                                                              aGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                                                                        >  1:1314403/1‑150 (MQ=255)
                                                                   gcgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                                                                   <  2:519023/150‑1 (MQ=255)
                                                                    cgATTTTAGGATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                                                                  <  1:305488/150‑1 (MQ=255)
                                                                    cgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGGCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGTGCCTCTATGGGCTACAAAGTCATTATCggg                                                                                  <  1:1152806/150‑1 (MQ=255)
                                                                     gATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                                                                 >  1:1813042/1‑150 (MQ=255)
                                                                     gATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCGCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                                                                 >  2:1370304/1‑150 (MQ=255)
                                                                       ttttAGCATGATCCGCAGACGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                                                                               >  1:1837032/1‑150 (MQ=255)
                                                                        tttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                                                                              <  2:865255/150‑1 (MQ=255)
                                                                           aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                                                                           >  1:808284/1‑150 (MQ=255)
                                                                            gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCGGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGCCTACAAAGTCATTATCGGGGATGGtta                                                                          >  2:1268241/1‑150 (MQ=255)
                                                                               tGATCCGCAGAAGCTAACGTAATTGTCAGACAGGGAGTGGCTGGTACATATATGGCAAACGCCCGGAAAATCCTGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGCGCCTCTATGGGCTACAAAGTCATTAACGGGGATGGttattt                                                                       >  1:51764/1‑150 (MQ=255)
                                                                                 aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGATACAAAGTCATTATCGGGGATGGttattttt                                                                     >  1:1898328/1‑149 (MQ=255)
                                                                                  tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                                                                    >  2:1263658/1‑150 (MQ=255)
                                                                                   ccGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                                                                   >  2:5108/1‑150 (MQ=255)
                                                                                    ctcAGATTCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATTAATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                                                                  <  1:1428253/148‑1 (MQ=255)
                                                                                       aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCa                                                               <  1:1447505/150‑1 (MQ=255)
                                                                                         aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAt                                                             >  2:1386880/1‑150 (MQ=255)
                                                                                                cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg                                                      <  2:1882054/150‑1 (MQ=255)
                                                                                                cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg                                                      >  1:436316/1‑150 (MQ=255)
                                                                                                   aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa                                                   >  1:1632961/1‑150 (MQ=255)
                                                                                                        tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATgg                                              <  1:448475/150‑1 (MQ=255)
                                                                                                         cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGc                                             >  1:18692/1‑150 (MQ=255)
                                                                                                          cggcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCTGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCa                                            <  2:1898070/147‑1 (MQ=255)
                                                                                                           gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGTAAAAACCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGACATTATCGGGGATGGTTATTTTATTGTCAATTTATCAGCGATATGGCat                                           >  2:1474253/1‑150 (MQ=255)
                                                                                                           gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCat                                           >  1:671248/1‑150 (MQ=255)
                                                                                                           gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCat                                           >  1:585622/1‑150 (MQ=255)
                                                                                                                 taGTTGCTGGTACATATATGGCATACGCCCGGAAATTCCGGGATTCGCAGGTCGCTAATGTGGATAAAACTTAGGTCCTCTATGGGCTACAAAGTCATTATAGGGGTTGGGTATTTTATTGTCAATTTATGAGCGATATGGCattgattg                                     <  1:930683/149‑1 (MQ=255)
                                                                                                                  aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAAGAGCGATATGGCAttgattga                                    <  2:1676476/150‑1 (MQ=255)
                                                                                                                   gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGAc                                   <  1:586294/150‑1 (MQ=255)
                                                                                                                    tGGCTGGTACATATATGGCAAAAGCCCGGAACATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACt                                  <  2:1126942/150‑1 (MQ=255)
                                                                                                                    tGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTAATTGTCAATTTATGAGAGATATGGCATTGATTTACt                                  >  1:1193384/1‑150 (MQ=255)
                                                                                                                     ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTTTTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACtt                                 >  2:1430341/1‑150 (MQ=255)
                                                                                                                     ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACtt                                 >  1:1059416/1‑150 (MQ=255)
                                                                                                                     ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACtt                                 >  2:1154720/1‑150 (MQ=255)
                                                                                                                       cTGTTACATATATGGCAAAAGCCAGGAATATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTTTTGTCATTTTTTGAGCGATATGTCTTTGATTGTCTtga                               <  2:254594/150‑1 (MQ=255)
                                                                                                                       cTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga                               >  1:418150/1‑150 (MQ=255)
                                                                                                                        tGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtgat                              >  2:1775703/1‑150 (MQ=255)
                                                                                                                           tACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAATTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGAc                           >  2:1210762/1‑150 (MQ=255)
                                                                                                                            aCATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTTTCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGAcc                          >  1:255863/1‑150 (MQ=255)
                                                                                                                             cATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCg                         >  2:608707/1‑150 (MQ=255)
                                                                                                                                    ggCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTg                  <  2:224019/150‑1 (MQ=255)
                                                                                                                                    ggCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTg                  <  2:1399869/150‑1 (MQ=255)
                                                                                                                                    ggCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGACAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTg                  <  2:367052/150‑1 (MQ=255)
                                                                                                                                    ctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTg                  >  1:1638065/6‑150 (MQ=255)
                                                                                                                                     tgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGt                 >  1:580709/5‑150 (MQ=255)
                                                                                                                                      cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTa                <  1:1448592/150‑1 (MQ=255)
                                                                                                                                       aaaaGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTAt               <  1:1607179/150‑1 (MQ=255)
                                                                                                                                        aaaGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATg              <  1:490763/150‑1 (MQ=255)
                                                                                                                                          aGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTc            <  1:520923/150‑1 (MQ=255)
                                                                                                                                          aGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTc            <  1:1779629/150‑1 (MQ=255)
                                                                                                                                           gCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCt           >  1:531197/1‑150 (MQ=255)
                                                                                                                                             ccGGCAACTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATCGGCGACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGc         <  2:383611/150‑1 (MQ=255)
                                                                                                                                             ccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGTTGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGCCCGGCCTCTGTTTGTCTgg         >  2:1357556/1‑149 (MQ=255)
                                                                                                                                              cGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGca        >  1:1777887/1‑150 (MQ=255)
                                                                                                                                              cGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGca        <  2:729396/150‑1 (MQ=255)
                                                                                                                                              cGGAAAATCCGGGCTTAGCAGGTACCTCAAGGGGTTAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGTATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGca        <  1:41667/150‑1 (MQ=255)
                                                                                                                                               ggAATATCCGTGCTTCTCATGTACCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACATAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaa       <  1:250348/150‑1 (MQ=255)
                                                                                                                                                  aaaGCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaacca    <  2:1835821/150‑1 (MQ=255)
                                                                                                                                                    aTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaaccaac  >  2:1696475/1‑150 (MQ=255)
                                                                                                                                                    aTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaaccaaa  >  1:321935/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAAC  >  NC_000913/3959778‑3960075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: