Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,967,404 G→T 6.9% V330F (GTT→TTT)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,967,4040GT 6.9% 367.0 / 9.6 127V330F (GTT→TTT) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (53/64);  new base T (4/6);  total (57/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAA  >  NC_000913/3967256‑3967553
                                                                                                                                                    |                                                                                                                                                     
        aaGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCATCTTTGGTGCGGCGCGTAACGTGGAAGAGGGAGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACg                                                                                                                                              <  2:529208/150‑1 (MQ=255)
           tgtTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAag                                                                                                                                           <  1:1675207/150‑1 (MQ=255)
           tgtTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAag                                                                                                                                           <  1:1408282/150‑1 (MQ=255)
               gACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGtt                                                                                                                                       <  1:1356317/150‑1 (MQ=255)
                 ccGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAAAGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTTTCGGGACGGCGCTTTTCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTa                                                                                                                                     >  1:160576/1‑150 (MQ=255)
                   ggtggtGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTaaa                                                                                                                                   <  1:1154981/150‑1 (MQ=255)
                    gtggtgTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAg                                                                                                                                  <  1:1260174/150‑1 (MQ=255)
                    gtggtgTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAg                                                                                                                                  <  2:815246/150‑1 (MQ=255)
                        tgtgGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACAATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAc                                                                                                                              <  1:248067/150‑1 (MQ=255)
                          tgGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAg                                                                                                                            >  1:921849/1‑150 (MQ=255)
                           gtaTGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCCGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAgg                                                                                                                           <  2:912015/148‑1 (MQ=255)
                           ggATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAgg                                                                                                                           >  2:1724729/1‑150 (MQ=255)
                           ggATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGAGAGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGCCGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAgc                                                                                                                           >  1:487379/1‑149 (MQ=255)
                             aTGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCa                                                                                                                         <  1:1371354/150‑1 (MQ=255)
                              tGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCaa                                                                                                                        >  1:1471924/1‑150 (MQ=255)
                               gCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGCGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAATATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAAc                                                                                                                       >  1:1706023/1‑150 (MQ=255)
                                ccAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCCGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTATAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACa                                                                                                                      >  1:1805716/1‑150 (MQ=255)
                                ccAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACa                                                                                                                      >  1:1160770/1‑150 (MQ=255)
                                  aaCGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATg                                                                                                                    >  2:1760078/1‑150 (MQ=255)
                                   aCGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATgg                                                                                                                   <  2:1783628/150‑1 (MQ=255)
                                       ccTGCATCGTGCGAAACGCTTCTTTGGTGCGGCGCGTAAGGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTATAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACt                                                                                                               <  2:1545821/150‑1 (MQ=255)
                                       ccTGCATCGTCCGACACGCTTCTTTGGAGCTGCGCGTAACGTGGAAGCGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATTGAACt                                                                                                               <  2:234937/150‑1 (MQ=255)
                                         tGCATCGTCCGAAACGCTTTTTTGGGGCGGCGCGTAACGTGTAAGTGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAATATTTTAAAGGTACAGGCAACATGGAACTGc                                                                                                             <  2:1542195/150‑1 (MQ=255)
                                          gCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCa                                                                                                            >  2:144307/1‑150 (MQ=255)
                                          gCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCa                                                                                                            >  1:847744/1‑150 (MQ=255)
                                           cATCGTCCGAAACTCTTCTTTGGAGCGGCGCTTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCAc                                                                                                           <  2:108533/150‑1 (MQ=255)
                                            aTCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCAcc                                                                                                          <  1:175983/150‑1 (MQ=255)
                                            aTCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGAAGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCAcc                                                                                                          >  2:452852/1‑150 (MQ=255)
                                             tCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACct                                                                                                         >  1:403371/1‑150 (MQ=255)
                                                tCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCCGCCTGACCATTATCGCGCCGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACctctc                                                                                                      >  1:460541/1‑150 (MQ=255)
                                                 ccGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGCCCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGTCGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACctcgct                                                                                                     >  2:171205/1‑150 (MQ=255)
                                                  cGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTATAAAGGTACAGGCAACATGGAACTGCACCtcactc                                                                                                    >  2:1474176/1‑150 (MQ=255)
                                                  cGAAAAGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTGCAGGCAACATGGAACTGCACCcctctc                                                                                                    >  1:1497322/1‑150 (MQ=255)
                                                   gAAACGCTTCTTTGGTGCGGCGCGTCACTTGGAAGACGGCTGCAGCCTGACCATTCTCGCGACGGCGCTTATCGATACCGGTACTAAAAGGGACGAAGTTAGCTACGAAGAGTTTAAAGTTACAGGCAACATGGAACTGCACCTCTCTCg                                                                                                   <  1:79940/150‑1 (MQ=255)
                                                     aaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTa                                                                                                 <  1:144307/150‑1 (MQ=255)
                                                     aaCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTa                                                                                                 <  2:732266/150‑1 (MQ=255)
                                                      aCGCTTCTTTGGTGCGGCTCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCGTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTaa                                                                                                <  1:590664/150‑1 (MQ=255)
                                                       cgcgTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTGATCTACGAAGAGTTTAAAGGGACAGGCAACATGGAACTGCACCTCTCTCGTAAg                                                                                               <  2:967184/150‑1 (MQ=255)
                                                       cGCTTCTTTGGTGCGGCGCGTAACGTGTAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAg                                                                                               <  2:1199297/150‑1 (MQ=255)
                                                       cGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAg                                                                                               <  2:1289672/150‑1 (MQ=255)
                                                       cGCTTCTTTGGGGCGGCGCGTAAGGTGGAAGAGGCCGGCAGCCTGACCATTATCGCGAGGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTAAGTTGAGTTTAAAGGTACATGCAACATGGCACTGCACCACTCTCGTatg                                                                                               <  2:306795/150‑3 (MQ=255)
                                                         cttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGAt                                                                                             <  2:669373/150‑1 (MQ=255)
                                                           tcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTATAATGGACGAAGTTATCTACGAAGTGTTTAAAGGTACAGGCAACATGGTACTGCACCTCTCTCGTAAGATCg                                                                                           <  1:793953/150‑1 (MQ=255)
                                                              ttGGTGCGGCGCGTAACGTGGAAGAGGGTGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTg                                                                                        <  1:546059/150‑1 (MQ=255)
                                                              ttGGTGCCGCGCGTAACGGGGAAGAGGGCGGCAGCCTGACCATTCTCGCGACGGCGCTTATCGATACCGTTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTg                                                                                        <  2:291807/150‑1 (MQ=255)
                                                                   gcggcgCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGaaaaa                                                                                   >  1:154944/1‑150 (MQ=255)
                                                                   gcggcgCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGaaaaa                                                                                   >  2:1880512/1‑150 (MQ=255)
                                                                     ggcgCGTAACGGGGAAGAGGGCGGCAGGCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcg                                                                                 <  2:351165/150‑1 (MQ=255)
                                                                        gcgTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGt                                                                              <  1:834871/150‑1 (MQ=255)
                                                                          gTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGTTGAAAAACGCGTCt                                                                            >  2:1458714/1‑150 (MQ=255)
                                                                               gTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCg                                                                       >  1:953534/1‑150 (MQ=255)
                                                                               gTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCg                                                                       >  2:1788048/1‑150 (MQ=255)
                                                                                tGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGTAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCgg                                                                      >  2:1473738/1‑150 (MQ=255)
                                                                                 ggAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGc                                                                     >  1:604066/1‑150 (MQ=255)
                                                                                   aaGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTa                                                                   <  1:1749719/150‑1 (MQ=255)
                                                                                       gggCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCgc                                                               >  1:1818218/1‑149 (MQ=255)
                                                                                         gcgggAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACt                                                             <  2:1443439/150‑1 (MQ=255)
                                                                                         gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTGTATAAGGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGCAAAACGCGTCTTCCCGGCTATCGACt                                                             >  1:303942/1‑150 (MQ=255)
                                                                                         gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACt                                                             <  2:15854/150‑1 (MQ=255)
                                                                                         gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACt                                                             <  2:1546107/150‑1 (MQ=255)
                                                                                         gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACt                                                             <  2:452685/150‑1 (MQ=255)
                                                                                           ggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTAc                                                           <  1:1736022/150‑1 (MQ=255)
                                                                                           ggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTAc                                                           <  2:1683824/150‑1 (MQ=255)
                                                                                            gcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTTAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACa                                                          <  2:404111/150‑1 (MQ=255)
                                                                                            gcAGCCGGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACa                                                          <  2:411099/150‑1 (MQ=255)
                                                                                              aGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAc                                                        <  2:1828491/150‑1 (MQ=255)
                                                                                               gCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACCAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAcc                                                       >  1:663925/1‑150 (MQ=255)
                                                                                                ccTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTATCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCg                                                      >  1:163002/1‑150 (MQ=255)
                                                                                                 cTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGt                                                     >  2:225631/1‑150 (MQ=255)
                                                                                                     ccATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTg                                                 <  1:1891578/150‑1 (MQ=255)
                                                                                                      cATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTgg                                                <  1:1512553/150‑1 (MQ=255)
                                                                                                      cATTATCGCGACGGCGATTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCGATCGACTACAACCGTTCTgg                                                <  2:1493265/150‑1 (MQ=255)
                                                                                                         tATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGTTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAc                                             >  1:556418/1‑150 (MQ=255)
                                                                                                           tCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAccc                                           <  1:386646/150‑1 (MQ=255)
                                                                                                            cgcgACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCg                                          <  1:782531/150‑1 (MQ=255)
                                                                                                            cgcgACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCg                                          <  1:405019/150‑1 (MQ=255)
                                                                                                            cgcgACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGATCTGGTACCCg                                          >  2:1053650/1‑150 (MQ=255)
                                                                                                            cgcgACCGCGCTTTTCGATACCGCTGCTATAATCGACGAAGTTATCTAGTAAGAGTTAAAAGGTACAGGCAACATGCAACTGCACCTCTCTCGGAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTGCTGGTACCCg                                          <  1:310713/150‑1 (MQ=255)
                                                                                                                    cgcTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCATCATGGAACTGCACCTCTCTCGTAAGATCGATGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAaagaag                                  <  2:1124598/150‑1 (MQ=255)
                                                                                                                    cgcTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAaagaag                                  >  2:675618/1‑150 (MQ=255)
                                                                                                                      cgtATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGTTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAagag                                <  2:143678/148‑1 (MQ=255)
                                                                                                                      attATCGATACAGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGTTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAagag                                <  2:149276/149‑1 (MQ=255)
                                                                                                                       ttATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgc                               <  1:910394/150‑1 (MQ=255)
                                                                                                                       ttATCGATACCGGTGCTAAAATGCACGAAGTTATCTACGCAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgc                               <  2:1678955/150‑1 (MQ=255)
                                                                                                                        tATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgct                              >  2:1280704/1‑150 (MQ=255)
                                                                                                                            gATACCGGTTCTAAAATGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc                          >  1:1829541/1‑150 (MQ=255)
                                                                                                                            gATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAACCGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc                          >  1:1387246/1‑150 (MQ=255)
                                                                                                                              tACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                        <  2:1471924/150‑1 (MQ=255)
                                                                                                                              tACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                        <  2:1479226/150‑1 (MQ=255)
                                                                                                                              tACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                        <  2:1721996/150‑1 (MQ=255)
                                                                                                                               acaggTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg                       <  2:1703551/147‑1 (MQ=255)
                                                                                                                                 cGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAATGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGAc                     <  2:95266/150‑1 (MQ=255)
                                                                                                                                 cGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGAc                     >  1:1586831/1‑150 (MQ=255)
                                                                                                                                  ggTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGAca                    >  1:1864027/1‑149 (MQ=255)
                                                                                                                                     tCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAg                 >  2:1004416/1‑150 (MQ=255)
                                                                                                                                      cTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCCCGACTCAgg                >  1:61452/1‑150 (MQ=255)
                                                                                                                                            tGGACTAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAAc          <  1:1262942/150‑1 (MQ=255)
                                                                                                                                              gACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTg        <  1:1094441/150‑1 (MQ=255)
                                                                                                                                               aCGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc       >  1:735112/1‑150 (MQ=255)
                                                                                                                                                    gTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGTTTAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaa  <  2:1160770/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAA  >  NC_000913/3967256‑3967553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: