Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,051,259 G→T 5.6% noncoding (224/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,2590GT 5.6% 430.2 / 11.1 142noncoding (224/245 nt)csrCCsrC sRNA sequesters CsrA, a carbon flux regulator
Reads supporting (aligned to +/- strand):  ref base G (73/61);  new base T (3/5);  total (76/66)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 145 total aligned reads displayed.

GTCAGGAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACG  >  NC_000913/4051111‑4051408
                                                                                                                                                    |                                                                                                                                                     
gTCAGGAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGt                                                                                                                                                      >  1:1309504/1‑150 (MQ=255)
       aGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGt                                                                                                                                               >  2:354041/1‑150 (MQ=255)
         aCAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCg                                                                                                                                             <  2:194281/150‑1 (MQ=255)
          cAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGc                                                                                                                                            >  2:1053701/1‑150 (MQ=255)
           aaaCGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                                                                           <  2:441492/150‑1 (MQ=255)
              cGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCttt                                                                                                                                        <  2:14430/150‑1 (MQ=255)
                tCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCttttt                                                                                                                                      <  1:1248984/150‑1 (MQ=255)
                tCCGGAGACGTAATTAAACGGAAATGGAATAAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGTTTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGAATGAGTAATCTGTCGCCttttt                                                                                                                                      >  2:245236/1‑150 (MQ=255)
                 ccGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttttt                                                                                                                                     >  2:335085/1‑150 (MQ=255)
                 ccGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtttttt                                                                                                                                     <  1:823350/150‑1 (MQ=255)
                   ggAGACGTAAATAAACGGAAATGGACACAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCTTTTTTcc                                                                                                                                   <  1:1856992/150‑2 (MQ=39)
                    gagaCGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCtt                                                                                                                                  <  1:1904449/150‑1 (MQ=255)
                      gaCGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTATGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttg                                                                                                                                >  1:985482/1‑150 (MQ=255)
                      gaCGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTATGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttg                                                                                                                                >  1:978779/1‑150 (MQ=255)
                        cGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                                                                              >  2:1152773/1‑150 (MQ=255)
                          tAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttg                                                                                                                            <  2:1583122/150‑1 (MQ=255)
                           aaTTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttgc                                                                                                                           <  1:1536973/150‑1 (MQ=255)
                            aTTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTtgcttgct                                                                                                                          >  2:1667672/1‑150 (MQ=255)
                            aTTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTtgcttgct                                                                                                                          <  1:1842960/150‑1 (MQ=255)
                             ttAAACGGAAATGTAATCAACACGGTTTCTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTgcttgctt                                                                                                                         <  1:199140/150‑1 (MQ=255)
                               aaaCGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTc                                                                                                                       >  2:1614813/1‑150 (MQ=255)
                                    gAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                                                                  <  1:1226122/150‑1 (MQ=255)
                                    gAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                                                                  >  2:390628/1‑150 (MQ=255)
                                    gAAATGGAATCAACACGGATGGTTCCGGCTAAAGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                                                                  <  1:1317156/150‑1 (MQ=255)
                                     aaaTGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAg                                                                                                                 <  1:987572/150‑1 (MQ=255)
                                     aaaTGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAg                                                                                                                 <  1:1521942/150‑1 (MQ=255)
                                            aTCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgc                                                                                                          <  2:1694470/150‑1 (MQ=255)
                                               aaCACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCcgccgc                                                                                                       >  1:432458/1‑150 (MQ=255)
                                                 cacGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTAGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                                                                     >  2:88217/1‑150 (MQ=255)
                                                    ggATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATc                                                                                                  <  1:1827300/150‑1 (MQ=255)
                                                        tgatCCGGCTAAAGGAAAAACAGTGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAAACTGTCGCCTTTTTTCTTTGCTAGCTTTCTGTTAGATTCCGCCGCAAATCtata                                                                                              <  1:245236/147‑1 (MQ=255)
                                                        tGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtata                                                                                              <  2:1360156/150‑1 (MQ=255)
                                                         ttccgggCTAACGCAAACACCGGGTGTGTTGGCGGCCTGCATGGTTTGTATGACCCGTTACGGGTTTTGAGTCAGGAAAAACGGCGACTGTGTAATCTGGCGCCTTTTTTGTTTGCTTGCTTTCTGTTCGATTCCGCCGCAAATCTATAc                                                                                             <  2:411346/145‑1 (MQ=255)
                                                         gTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGGTTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATAc                                                                                             <  1:1486758/150‑1 (MQ=255)
                                                            ccGGCTAAAGGAAAATCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                                                                          >  1:101023/1‑150 (MQ=255)
                                                            ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                                                                          >  2:1543627/1‑150 (MQ=255)
                                                            ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                                                                          >  1:260712/1‑150 (MQ=255)
                                                            ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                                                                          >  2:451849/1‑150 (MQ=255)
                                                              ggCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAAt                                                                                        <  1:1040105/150‑1 (MQ=255)
                                                              ggCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAAt                                                                                        >  1:668515/1‑150 (MQ=255)
                                                                  aaaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATaaaa                                                                                    >  2:1055881/1‑150 (MQ=255)
                                                                   aaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTGTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                                                                                   >  1:1445789/1‑150 (MQ=255)
                                                                    agtataaaCAGGGTGTGTTGGCGGACTGGAAGGATTGTAAGACCGGTTAAGGGTTATGAGTCAGGTAAAAAGTCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACg                                                                                  <  1:63684/145‑1 (MQ=255)
                                                                    aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACg                                                                                  >  2:1559959/1‑150 (MQ=255)
                                                                    aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACg                                                                                  >  2:1771504/1‑150 (MQ=255)
                                                                      gAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGc                                                                                >  1:779843/1‑150 (MQ=255)
                                                                             tgggTGTGTTGGGGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCATGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGa                                                                         <  2:186507/149‑1 (MQ=255)
                                                                               ggtggggtggCGGCCTGCAAGGAGTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                                                                       <  2:1584005/150‑1 (MQ=255)
                                                                               ggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                                                                       <  1:1140102/150‑1 (MQ=255)
                                                                               ggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                                                                       <  2:1865798/150‑1 (MQ=255)
                                                                               ggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                                                                       >  1:1682731/1‑150 (MQ=255)
                                                                                   ggtTGGCGGCCTGCAAGGATTGTAAGACCAGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAAcc                                                                   <  1:940093/149‑1 (MQ=255)
                                                                                       ggcggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCATTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAATCGGCTAAAAGACGAACCATTa                                                               <  1:256620/150‑1 (MQ=255)
                                                                                       ggcggcCAGCAAGGAATGTAAGACCCGTTAAGCGTTATGAGTCAGGTAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTACTTTGCTTGCTTTCTGTTTGATTCCTCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCAGTa                                                               >  2:1234379/1‑150 (MQ=255)
                                                                                          ggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa                                                           >  2:535604/1‑150 (MQ=255)
                                                                                           gcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCATGAAAAAAGGCTACAGAGTAATCTGTAGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa                                                           <  1:93652/150‑1 (MQ=255)
                                                                                             cTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAc                                                         <  1:108238/150‑1 (MQ=255)
                                                                                                  aGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca                                                    >  1:373608/1‑150 (MQ=255)
                                                                                                  aGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca                                                    <  1:32631/150‑1 (MQ=255)
                                                                                                   ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatcat                                                   <  2:958945/150‑1 (MQ=255)
                                                                                                     aTTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCt                                                 >  1:1397455/1‑150 (MQ=255)
                                                                                                      ttGTAAGACCAGTTAAGGGTTATGAGTCATGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCtt                                                <  1:708024/150‑1 (MQ=255)
                                                                                                       tGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTc                                               >  1:54112/1‑150 (MQ=255)
                                                                                                        gTAAGACAAGTTTAGGGTTATGAGTCAGGAAATAAGGATACATAGTAATCTGTCGCCTTTTTTCTTTTCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCa                                              <  2:1267767/150‑1 (MQ=255)
                                                                                                         tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCaa                                             <  2:76180/150‑1 (MQ=255)
                                                                                                            gACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGATGAACCATTATGAAACCATCATCTTCAAACt                                          <  2:1309504/150‑1 (MQ=255)
                                                                                                            gACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACt                                          >  1:37451/1‑150 (MQ=255)
                                                                                                             aCCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATTCTGAATAAAACGGCGTAAAGACGAACCATTAAGAAACCATCATCTTCAAACta                                         >  2:1608579/1‑149 (MQ=255)
                                                                                                              cccGTTAATGGTTATGAGTCAGGAAAAAACGCGACAGAGTAATCTGTCGCCTTTTTTATTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTTAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCa                                        <  2:899140/150‑1 (MQ=255)
                                                                                                              cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCa                                        <  1:1497972/150‑1 (MQ=255)
                                                                                                              cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCa                                        >  2:536533/1‑150 (MQ=255)
                                                                                                              cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCa                                        >  2:457542/1‑150 (MQ=255)
                                                                                                               ccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTCCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCAc                                       <  1:354041/150‑1 (MQ=255)
                                                                                                                cGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACg                                      <  2:1232297/150‑1 (MQ=255)
                                                                                                                cGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACg                                      >  2:818128/1‑150 (MQ=255)
                                                                                                                cGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACg                                      >  1:1828930/1‑150 (MQ=255)
                                                                                                                cGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTATTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAATGACGAACCATTATGAAACCATCATCTTCAAACTCACg                                      >  1:1000563/1‑150 (MQ=255)
                                                                                                                  ttAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACgca                                    <  2:1575723/150‑1 (MQ=255)
                                                                                                                       ggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTATTTTCTTGCTTTATGTTATATTACGCCGCAAATCTATAATGAATAAAACCGCTAAAAGACGAACCAATATGAAACCATCATCTTCAAACTAAAGCAGCaaa                               >  2:1413502/1‑150 (MQ=255)
                                                                                                                          tATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGCACCATTATGAAACCATCATCTTCAAACTCACGCAGCATAGGt                            >  1:1236513/1‑150 (MQ=255)
                                                                                                                               gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGc                       <  2:1151032/150‑1 (MQ=255)
                                                                                                                               gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGc                       >  1:260786/1‑150 (MQ=255)
                                                                                                                                tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCa                      <  2:1802663/150‑1 (MQ=255)
                                                                                                                                 cAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaa                     >  2:1131935/1‑150 (MQ=255)
                                                                                                                                  aGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaaa                    >  2:1470469/1‑150 (MQ=255)
                                                                                                                                   ggAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCGGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAACGGTCAGGCaaaa                   >  1:316368/1‑150 (MQ=255)
                                                                                                                                   ggAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaaaa                   >  1:753039/1‑150 (MQ=255)
                                                                                                                                   ggAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCaaaa                   >  1:667669/1‑150 (MQ=255)
                                                                                                                                      aaaaaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCTGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAgcg                <  1:666200/150‑1 (MQ=255)
                                                                                                                                        aaaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGTCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGGCATGCAAAAGcgcg              <  2:1415754/150‑1 (MQ=255)
                                                                                                                                           ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAAACATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCg           >  1:1250938/1‑150 (MQ=255)
                                                                                                                                           ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTACGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCg           >  1:641134/1‑150 (MQ=255)
                                                                                                                                           ggCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCg           <  2:755434/150‑1 (MQ=255)
                                                                                                                                            gCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGa          >  1:1815365/1‑150 (MQ=255)
                                                                                                                                            gCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGa          >  2:1837655/1‑150 (MQ=255)
                                                                                                                                            gCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGa          <  1:1366144/150‑1 (MQ=255)
                                                                                                                                             cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGAGCGTCGaa         >  2:1596028/1‑150 (MQ=255)
                                                                                                                                                  gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGATCCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAacc    >  1:1543568/1‑149 (MQ=255)
                                                                                                                                                    gTAATCTGTCGCCTTTTTTCTTTGCTTTCTTTCTGTTAGATTCCGCCGCAAATCAATTCTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCCTCGAAAAACAcg  >  2:1598975/1‑150 (MQ=255)
                                                                                                                                                    gTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACAcg  >  2:1121222/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCAGGAAGACAAACGTCCGGAGACGTAATTAAACGGAAATGGAATCAACACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCATCTTCAAACTCACGCAGCAAAGGTCATGCAAAAGCGCGTCGAAAAACACG  >  NC_000913/4051111‑4051408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: