Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,062,811 C→A 5.9% L189M (CTG→ATG) ‡ yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,062,8110CA 5.9% 372.2 / 7.5 127L189M (CTG→ATG) ‡yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (57/61);  new base A (7/2);  total (64/63)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

AAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTG  >  NC_000913/4062662‑4062960
                                                                                                                                                     |                                                                                                                                                     
caaCAGGGCGGGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGGcc                                                                                                                                                       <  1:1853544/149‑1 (MQ=255)
     gggCGTGGCCTTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGGTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAAt                                                                                                                                                  <  2:1663962/150‑1 (MQ=255)
      ggCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATg                                                                                                                                                 <  1:1315307/150‑1 (MQ=255)
         gTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACa                                                                                                                                              <  1:717036/150‑1 (MQ=255)
           ggCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACaaa                                                                                                                                            >  1:1502002/1‑150 (MQ=255)
            gCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaag                                                                                                                                           >  1:1280199/1‑150 (MQ=255)
             cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaaga                                                                                                                                          <  2:1009156/150‑1 (MQ=255)
             cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaaga                                                                                                                                          <  2:1140717/150‑1 (MQ=255)
               ttGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGAAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTATTTGTCCTGAATGACAaagaac                                                                                                                                        >  2:965290/1‑149 (MQ=255)
                tttCGATACAGTTTTTGAATTTTCTGCGTTTGCATTATTTACATGGTTTTGCAGTGGCTTATTATGTTTTAAAGCAGGCATCTGTTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       <  1:388445/148‑1 (MQ=255)
                tGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGTCTTATTAGGATTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTTCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       >  1:975019/1‑150 (MQ=255)
                tGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       >  1:1150513/1‑150 (MQ=255)
                tGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       <  1:1402503/150‑1 (MQ=255)
                 gTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTTGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAagag                                                                                                                                      <  1:329677/150‑1 (MQ=255)
                  tCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAgg                                                                                                                                     <  1:1731004/150‑1 (MQ=255)
                  tCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAgg                                                                                                                                     <  1:628273/150‑1 (MQ=255)
                   cGATTCCGTGGTGGGATTTTCTGCGTTGGCAGTATTTACCTGGCTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGACCTGAATGACAAAGAAGAGGc                                                                                                                                    <  1:212760/150‑1 (MQ=255)
                    gATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaagaaaataat                                                                                                                                   <  2:1761602/150‑7 (MQ=255)
                        ccGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTACCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgt                                                                                                                               >  2:1500182/1‑150 (MQ=255)
                          gtggtgGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtcc                                                                                                                             >  1:9438/1‑150 (MQ=255)
                          gtggtgGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtcc                                                                                                                             >  2:63519/1‑150 (MQ=255)
                           tggttgCATTTTCTGCGTTGGCAGTATTTACCTGGTTGGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtccg                                                                                                                            <  1:244497/150‑1 (MQ=255)
                           tggtggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtccg                                                                                                                            <  2:940912/150‑1 (MQ=255)
                             gtggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCACTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATGAATGACAAAGAATAGGCACCGTCCGtt                                                                                                                          >  1:929554/1‑150 (MQ=255)
                              tggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTg                                                                                                                         <  1:437543/150‑1 (MQ=255)
                               ggCATTTTCTGCGTTGGCATTATTTACCTGGTTTGGCAGTGGCTTATTATGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGa                                                                                                                        <  1:1487471/150‑1 (MQ=255)
                                gCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAg                                                                                                                       >  1:1848860/1‑150 (MQ=255)
                                 cATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAgg                                                                                                                      <  2:1491023/150‑1 (MQ=255)
                                        tGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGaaaaa                                                                                                               >  2:1750568/1‑150 (MQ=255)
                                          cGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaag                                                                                                             <  2:1637802/150‑1 (MQ=255)
                                           gTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaaga                                                                                                            <  1:1003354/150‑1 (MQ=255)
                                             tGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaagaag                                                                                                          <  2:1847020/150‑1 (MQ=255)
                                              ggCAGTATTTACCTGGTTTGCCTGTGGCTTATTAGGTTTTAAAGCAGGCTTCTGGTTCTATTACCTTATTGTGATTGACGTAGGAATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAagaaga                                                                                                         >  2:71293/1‑150 (MQ=255)
                                               gCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGAc                                                                                                        >  1:859575/1‑150 (MQ=255)
                                               gCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTAGAGGTGAAAAAAGAATAc                                                                                                        >  1:1043608/1‑150 (MQ=255)
                                                      ttACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCAt                                                                                                 >  1:614595/1‑150 (MQ=255)
                                                        aCCTGGTTTGTCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCg                                                                                               <  2:775999/150‑1 (MQ=255)
                                                        aCCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCg                                                                                               >  1:1629961/1‑150 (MQ=255)
                                                          cTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTGCTGAATGACAAATAAGATGCACCGTCCGTTGAGTTGAAAAAAGAAGACTGAGCATCGaa                                                                                             >  1:133092/1‑150 (MQ=255)
                                                            ggTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTTGTTCTCTTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAGGAAGACGGAGCATCGaaaa                                                                                           <  2:524661/150‑1 (MQ=255)
                                                              tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                         <  2:1225708/150‑1 (MQ=255)
                                                              tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                         <  2:34692/150‑1 (MQ=255)
                                                                 ggCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACAcc                                                                                      >  1:85868/1‑150 (MQ=255)
                                                                   cAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAg                                                                                    <  2:1371461/150‑1 (MQ=255)
                                                                    aGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGt                                                                                   <  1:623215/150‑1 (MQ=255)
                                                                    aGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGt                                                                                   <  1:1658581/150‑1 (MQ=255)
                                                                    aGTGGCTTATTAGGTGTTAAAGCAGGCATCTGGTTCTATTCCCTTAATGTGATTGCCGTAGGCATTATTATTATCTTTGTCCTGAATGACAAAGAAGCGGCACCGTCAGTTCAGGTGAAAAAAGAAGACGGAGAATCGAAAACCACCAGt                                                                                   >  2:349914/1‑150 (MQ=255)
                                                                       ggCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGATGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTAtt                                                                                >  1:524770/1‑149 (MQ=255)
                                                                       ggCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTTAAAAAAGAAGACTGAGATTAGAAAAACAACAGTAtt                                                                                >  1:1693039/1‑149 (MQ=255)
                                                                        gCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGa                                                                               >  1:892675/1‑150 (MQ=255)
                                                                        gCTTATTAGGTTTTAAAGCACGCATCTGGTACTATTCCCTTATTGTGATTGACGTAGGCATTATTATTTTCTTTTTCATGAATGACAAAGAAGAGTCACCGTCAGTTTAGTTGAAAAAATAAGACGGAGCATCGAAAAACACCAGTAtta                                                                               >  1:270868/1‑148 (MQ=255)
                                                                          ttattaGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGAcc                                                                             >  2:463602/1‑150 (MQ=255)
                                                                              ttggTTTTAAAGCAGGCAGCTGGTTATTTTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                         <  2:1200111/148‑1 (MQ=255)
                                                                              taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                         >  2:1021065/1‑150 (MQ=255)
                                                                              taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                         <  2:814389/150‑1 (MQ=255)
                                                                               acgTTTTAAAGGAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGt                                                                        <  1:511181/148‑1 (MQ=255)
                                                                               aGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACTAAGTAGAGGCACCGTCCGTTGAGGTGAAAAATGAAGACGGAGCATCGAAAAACACCAGTATGGCCTCGGt                                                                        >  1:743006/1‑150 (MQ=255)
                                                                               aGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTTAAAAAAGAAGACGGAGCATCGAAAAACACAAGTATGACCTCGGt                                                                        >  1:361755/1‑150 (MQ=255)
                                                                                ggTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTCGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTg                                                                       <  2:1394843/150‑1 (MQ=255)
                                                                                 gTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAATAAGACGGATCATCGAAAAACACCAGTATGACCTCGGTGc                                                                      >  1:1193879/1‑150 (MQ=255)
                                                                                 gTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGGAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGc                                                                      <  1:945227/150‑1 (MQ=255)
                                                                                  ttttAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTAATGAATGACAAAGAAGAGGCACCGTCAGTTGAGGTGAAAAAAGAAGACGGAGCATAGAAAAACACCACTATGACCTCGGTgat                                                                     >  1:344838/1‑148 (MQ=255)
                                                                                     tAAAGCATGCATCTGGTTATTATCCCTTATTGGGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaa                                                                  <  2:1387611/150‑1 (MQ=255)
                                                                                     tAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaa                                                                  >  2:1562396/1‑150 (MQ=255)
                                                                                      aaaGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaaa                                                                 >  2:1792909/1‑150 (MQ=255)
                                                                                        aGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGa                                                               <  2:1305450/150‑1 (MQ=255)
                                                                                        aGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGa                                                               <  2:1458544/150‑1 (MQ=255)
                                                                                         gCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGAc                                                              <  1:1287785/150‑1 (MQ=255)
                                                                                                tCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTa                                                       <  1:1409021/150‑1 (MQ=255)
                                                                                                    gTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTg                                                   <  2:823163/150‑1 (MQ=255)
                                                                                                       cTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCt                                                >  1:1640566/1‑150 (MQ=255)
                                                                                                       cTATACCCTTATTGTGATTGCCGTATGCATTATTATTTTCTTTGACCCGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCt                                                <  1:220753/150‑1 (MQ=255)
                                                                                                         aTTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTa                                              >  2:1229400/1‑150 (MQ=255)
                                                                                                               ttATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGACCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCtt                                        <  2:1837190/150‑1 (MQ=255)
                                                                                                                tATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCttt                                       >  1:606817/1‑150 (MQ=255)
                                                                                                                 aTTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCtttt                                      <  2:1502002/150‑1 (MQ=255)
                                                                                                                 aTTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCtttt                                      <  2:1720356/150‑1 (MQ=255)
                                                                                                                      gATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGt                                 >  1:869177/1‑150 (MQ=255)
                                                                                                                          gCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttc                             <  2:620116/150‑1 (MQ=255)
                                                                                                                          gCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttc                             >  1:901257/1‑150 (MQ=255)
                                                                                                                           ccGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttct                            >  1:1096319/1‑150 (MQ=255)
                                                                                                                            cGTAGGCTTTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGAACCGGCCGTTGAGGTGAAATAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTTTCGCTTTTAACGTcttctt                           <  1:1768333/150‑1 (MQ=255)
                                                                                                                              tAGGCTTTATTATTTTCTTTGTCCTGAATGACAATGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCg                         <  1:784635/150‑1 (MQ=255)
                                                                                                                              tAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCg                         >  2:357287/1‑150 (MQ=255)
                                                                                                                                 gCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTACAGTCTTCTTGGttt                      >  1:1143147/1‑150 (MQ=255)
                                                                                                                                  cATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTa                     >  1:1628224/1‑150 (MQ=255)
                                                                                                                                     tattattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgc                  <  2:1205108/150‑1 (MQ=255)
                                                                                                                                      attattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgcg                 <  2:606817/150‑1 (MQ=255)
                                                                                                                                         tttttCTTTGTCCTGAATGACAAAGAAGAGGAACCGTCCGTTGAGGTGAATAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGtt              <  1:965290/149‑1 (MQ=255)
                                                                                                                                           tttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTa            >  2:1586527/1‑150 (MQ=255)
                                                                                                                                           tttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTa            <  1:100629/150‑1 (MQ=255)
                                                                                                                                           cttCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAAAGTCTTATACTTTTACGCGGTTTa            >  1:424430/2‑150 (MQ=255)
                                                                                                                                            ttCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTaa           >  2:451311/1‑149 (MQ=255)
                                                                                                                                            ttCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACAATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTaa           >  1:837057/1‑149 (MQ=255)
                                                                                                                                             tCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCATTATGCCCTCGGTGCTGAAAGACTAAACTATCTGGCTTATCGCTATTAACGTCTTCTTCGTTTACTCGGTTTACt          >  2:353694/1‑150 (MQ=255)
                                                                                                                                              cTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtg         >  2:943770/1‑150 (MQ=255)
                                                                                                                                                  gTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGc     <  1:485143/150‑1 (MQ=255)
                                                                                                                                                   tCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTggac    >  1:1291493/1‑148 (MQ=255)
                                                                                                                                                   tCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAACACGGAGCATCGAAAAACACAAGTATGACCTAGGTGCTGAAATACAACACTATCTGGCTTATCTCTTTTAACTTCTTCTTCGTTTACGCGGTTTACTTTggac    >  2:1151424/1‑148 (MQ=255)
                                                                                                                                                     cTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTg  >  2:1068247/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTG  >  NC_000913/4062662‑4062960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: