Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,062,813 G→T 5.0% L189L (CTG→CTT) ‡ yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,062,8130GT 5.0% 387.0 / 7.4 128L189L (CTG→CTT) ‡yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (61/60);  new base T (4/3);  total (65/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 128 total aligned reads displayed.

CAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTG  >  NC_000913/4062665‑4062960
                                                                                                                                                    |                                                                                                                                                   
cAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGa                                                                                                                                                    >  1:1205108/1‑150 (MQ=255)
  gggCGTGGCCTTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGGTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAAt                                                                                                                                                  <  2:1663962/150‑1 (MQ=255)
   ggCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATg                                                                                                                                                 <  1:1884917/150‑1 (MQ=255)
   ggCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATg                                                                                                                                                 >  1:823163/1‑150 (MQ=255)
   ggCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATg                                                                                                                                                 <  2:1068553/150‑1 (MQ=255)
   ggCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATg                                                                                                                                                 <  1:1315307/150‑1 (MQ=255)
    gCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTAGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGa                                                                                                                                                >  2:1217196/1‑150 (MQ=255)
      gTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACa                                                                                                                                              <  1:717036/150‑1 (MQ=255)
        ggCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACaaa                                                                                                                                            >  1:1502002/1‑150 (MQ=255)
          cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaaga                                                                                                                                          <  2:1009156/150‑1 (MQ=255)
          cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaaga                                                                                                                                          <  2:1140717/150‑1 (MQ=255)
            ttGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGAAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTATTTGTCCTGAATGACAaagaac                                                                                                                                        >  2:965290/1‑149 (MQ=255)
             tttCGATACAGTTTTTGAATTTTCTGCGTTTGCATTATTTACATGGTTTTGCAGTGGCTTATTATGTTTTAAAGCAGGCATCTGTTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       <  1:388445/148‑1 (MQ=255)
             tGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGTCTTATTAGGATTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTTCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       >  1:975019/1‑150 (MQ=255)
             tGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       <  1:1402503/150‑1 (MQ=255)
             tGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAagaaga                                                                                                                                       >  1:1150513/1‑150 (MQ=255)
               tCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAgg                                                                                                                                     <  1:628273/150‑1 (MQ=255)
                  aTACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAc                                                                                                                                  <  2:1553619/150‑1 (MQ=255)
                     ccGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTACCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgt                                                                                                                               >  2:1500182/1‑150 (MQ=255)
                       gtggtgGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtcc                                                                                                                             >  1:9438/1‑150 (MQ=255)
                       gtggtgGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtcc                                                                                                                             >  2:63519/1‑150 (MQ=255)
                        tggttgCATTTTCTGCGTTGGCAGTATTTACCTGGTTGGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtccg                                                                                                                            <  1:244497/150‑1 (MQ=255)
                        tggtggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtccg                                                                                                                            <  2:940912/150‑1 (MQ=255)
                          gtggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCACTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATGAATGACAAAGAATAGGCACCGTCCGtt                                                                                                                          >  1:929554/1‑150 (MQ=255)
                           tggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTg                                                                                                                         <  1:437543/150‑1 (MQ=255)
                            ggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGa                                                                                                                        >  1:941017/1‑150 (MQ=255)
                             gCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAg                                                                                                                       >  1:1848860/1‑150 (MQ=255)
                              cATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAgg                                                                                                                      <  2:1491023/150‑1 (MQ=255)
                                     tGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGaaaaa                                                                                                               >  2:1750568/1‑150 (MQ=255)
                                        gTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaaga                                                                                                            <  1:1474947/150‑1 (MQ=255)
                                        gTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaaga                                                                                                            <  1:1003354/150‑1 (MQ=255)
                                          tGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaagaag                                                                                                          <  2:1847020/150‑1 (MQ=255)
                                            gCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTAGAGGTGAAAAAAGAATAc                                                                                                        >  1:1043608/1‑150 (MQ=255)
                                               gTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGa                                                                                                     <  2:1774862/150‑1 (MQ=255)
                                                   ttACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCAt                                                                                                 >  1:614595/1‑150 (MQ=255)
                                                     aCCTGGTTTGTCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCg                                                                                               <  2:775999/150‑1 (MQ=255)
                                                     aCCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCg                                                                                               >  1:1629961/1‑150 (MQ=255)
                                                      ccTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGa                                                                                              >  2:716200/1‑150 (MQ=255)
                                                       cTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTGCTGAATGACAAATAAGATGCACCGTCCGTTGAGTTGAAAAAAGAAGACTGAGCATCGaa                                                                                             >  1:133092/1‑150 (MQ=255)
                                                         ggTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTTGTTCTCTTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAGGAAGACGGAGCATCGaaaa                                                                                           <  2:524661/150‑1 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                         <  2:34692/150‑1 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                         <  2:1225708/150‑1 (MQ=255)
                                                              ggCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACAcc                                                                                      >  1:85868/1‑150 (MQ=255)
                                                              ggCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCCTCAGTTGAGGTGAAAAAAGAAGACGGAGCATAGAAAAACAcc                                                                                      >  2:1322951/1‑150 (MQ=255)
                                                                cAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAg                                                                                    <  2:1371461/150‑1 (MQ=255)
                                                                 aGTGGCTTTTTAGGGTTTAAAGCAGGCATGTGGGTCTATTCCCTTATTGTGATTGCCGGTGGCATTATATTTTCTTTGTCCTTAATGACAAAGAAGAGGAACCGTCAGTTGAGGTGAAAAAAGTAGACGGAGCATCGAAAAACACCAGTa                                                                                  <  2:1444551/150‑1 (MQ=255)
                                                                 aGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGt                                                                                   <  1:1658581/150‑1 (MQ=255)
                                                                 aGTGGCTTATTAGGTGTTAAAGCAGGCATCTGGTTCTATTCCCTTAATGTGATTGCCGTAGGCATTATTATTATCTTTGTCCTGAATGACAAAGAAGCGGCACCGTCAGTTCAGGTGAAAAAAGAAGACGGAGAATCGAAAACCACCAGt                                                                                   >  2:349914/1‑150 (MQ=255)
                                                                    ggCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGATGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTAtt                                                                                >  1:524770/1‑149 (MQ=255)
                                                                    ggCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTTAAAAAAGAAGACTGAGATTAGAAAAACAACAGTAtt                                                                                >  1:1693039/1‑149 (MQ=255)
                                                                     gCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGa                                                                               >  1:892675/1‑150 (MQ=255)
                                                                     gCTTATTAGGTTTTAAAGCACGCATCTGGTACTATTCCCTTATTGTGATTGACGTAGGCATTATTATTTTCTTTTTCATGAATGACAAAGAAGAGTCACCGTCAGTTTAGTTGAAAAAATAAGACGGAGCATCGAAAAACACCAGTAtta                                                                               >  1:270868/1‑148 (MQ=255)
                                                                       ttattaGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGAcc                                                                             >  2:463602/1‑150 (MQ=255)
                                                                           taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                         <  2:814389/150‑1 (MQ=255)
                                                                           taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                         >  2:1021065/1‑150 (MQ=255)
                                                                           taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                         <  1:871737/150‑1 (MQ=255)
                                                                            aGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGTCACCGTCCGTTGAGTTGAAAAAAGAAGACGGAGCATCTAAAAACACCAGTATGACATAGGt                                                                        >  1:367150/1‑150 (MQ=255)
                                                                            aGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTTAAAAAAGAAGACGGAGCATCGAAAAACACAAGTATGACCTCGGt                                                                        >  1:361755/1‑150 (MQ=255)
                                                                             ggTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTg                                                                       >  2:437809/1‑150 (MQ=255)
                                                                              gTTTTATAGAAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTTTCCTGAATGACAAAGAAGAGGCTCCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGc                                                                      <  1:248009/150‑1 (MQ=255)
                                                                              gTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAATAAGACGGATCATCGAAAAACACCAGTATGACCTCGGTGc                                                                      >  1:1193879/1‑150 (MQ=255)
                                                                              gTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGGAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGc                                                                      <  1:945227/150‑1 (MQ=255)
                                                                               ttttAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTAATGAATGACAAAGAAGAGGCACCGTCAGTTGAGGTGAAAAAAGAAGACGGAGCATAGAAAAACACCACTATGACCTCGGTgat                                                                     >  1:344838/1‑148 (MQ=255)
                                                                                  tAAAGCATGCATCTGGTTATTATCCCTTATTGGGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaa                                                                  <  2:1387611/150‑1 (MQ=255)
                                                                                  tAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaa                                                                  >  2:1562396/1‑150 (MQ=255)
                                                                                   aaaGCATGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaaa                                                                 <  1:1716043/150‑1 (MQ=255)
                                                                                   aaaGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaaa                                                                 >  2:1792909/1‑150 (MQ=255)
                                                                                     aGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGa                                                               <  2:1458544/150‑1 (MQ=255)
                                                                                      gCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGTCTCCGTCCGTTGAGGTGAATAAAGAAGGCGGAGCATCGATAAAGACCAGTATCCCCTCGGTGCTGAAAGAc                                                              >  1:69992/1‑150 (MQ=255)
                                                                                      gCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGAc                                                              <  1:1287785/150‑1 (MQ=255)
                                                                                       cAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACa                                                             >  1:447968/1‑150 (MQ=255)
                                                                                             tCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTa                                                       <  1:1409021/150‑1 (MQ=255)
                                                                                                 gTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTg                                                   <  2:823163/150‑1 (MQ=255)
                                                                                                    cTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCt                                                >  1:1640566/1‑150 (MQ=255)
                                                                                                    cTATACCCTTATTGTGATTGCCGTATGCATTATTATTTTCTTTGACCCGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCt                                                <  1:220753/150‑1 (MQ=255)
                                                                                                      aTTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTa                                              >  2:1229400/1‑150 (MQ=255)
                                                                                                            ttATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGACCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCtt                                        <  2:1837190/150‑1 (MQ=255)
                                                                                                             tATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCttt                                       >  1:606817/1‑150 (MQ=255)
                                                                                                              aTTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCtttt                                      <  2:1720356/150‑1 (MQ=255)
                                                                                                                   gATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGt                                 >  1:869177/1‑150 (MQ=255)
                                                                                                                       gCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttc                             >  1:556653/1‑150 (MQ=255)
                                                                                                                       gCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttc                             <  2:620116/150‑1 (MQ=255)
                                                                                                                        ccGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttct                            >  1:1096319/1‑150 (MQ=255)
                                                                                                                         cGTAGGCTTTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGAACCGGCCGTTGAGGTGAAATAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTTTCGCTTTTAACGTcttctt                           <  1:1768333/150‑1 (MQ=255)
                                                                                                                           tAGGCTTTATTATTTTCTTTGTCCTGAATGACAATGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCg                         <  1:784635/150‑1 (MQ=255)
                                                                                                                              gCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTACAGTCTTCTTGGttt                      >  1:1143147/1‑150 (MQ=255)
                                                                                                                               cATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTa                     >  1:1628224/1‑150 (MQ=255)
                                                                                                                                  tattattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgc                  <  2:1205108/150‑1 (MQ=255)
                                                                                                                                   attattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgcg                 <  2:606817/150‑1 (MQ=255)
                                                                                                                                        tttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTa            <  1:100629/150‑1 (MQ=255)
                                                                                                                                        tttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTa            >  2:1586527/1‑150 (MQ=255)
                                                                                                                                         ttCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTaa           >  2:451311/1‑149 (MQ=255)
                                                                                                                                         ttCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACAATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTaa           >  1:837057/1‑149 (MQ=255)
                                                                                                                                          tCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCATTATGCCCTCGGTGCTGAAAGACTAAACTATCTGGCTTATCGCTATTAACGTCTTCTTCGTTTACTCGGTTTACt          >  2:353694/1‑150 (MQ=255)
                                                                                                                                           cTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGCCGGAGCATCGAAAAACACCACTATGACCTCGGTGCTGAAAGACAAAACTATCTCGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtg         >  1:975812/1‑150 (MQ=255)
                                                                                                                                           cTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtg         >  2:943770/1‑150 (MQ=255)
                                                                                                                                               gTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGc     >  2:16414/1‑150 (MQ=255)
                                                                                                                                               gTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGc     <  1:485143/150‑1 (MQ=255)
                                                                                                                                                tCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTggac    >  1:1291493/1‑148 (MQ=255)
                                                                                                                                                tCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAACACGGAGCATCGAAAAACACAAGTATGACCTAGGTGCTGAAATACAACACTATCTGGCTTATCTCTTTTAACTTCTTCTTCGTTTACGCGGTTTACTTTggac    >  2:1151424/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTG  >  NC_000913/4062665‑4062960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: