Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,464 T→A 5.9% intergenic (‑63/+51) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4640TA 5.9% 219.9 / 2.8 78intergenic (‑63/+51)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base T (42/30);  new base A (3/2);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

GTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATA  >  NC_000913/4070315‑4070613
                                                                                                                                                     |                                                                                                                                                     
gTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                                                                                       <  2:1131240/150‑1 (MQ=255)
 tGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTg                                                                                                                                                      <  2:1524755/150‑1 (MQ=255)
   aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggg                                                                                                                                                    <  2:935292/150‑1 (MQ=255)
     aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTggggt                                                                                                                                                  >  1:1261069/1‑149 (MQ=255)
     aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                                  >  2:463868/1‑150 (MQ=255)
     aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                                  <  2:1075837/150‑1 (MQ=255)
        cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                                                                               <  1:1028346/150‑1 (MQ=255)
           ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                                                                                            <  2:451903/150‑1 (MQ=255)
           ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                                                                                            <  2:444738/150‑1 (MQ=255)
            cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGtt                                                                                                                                           <  1:1591283/150‑1 (MQ=255)
               ggCAAGTGAAACGGTCTGTCCACTTCAATGCATATTGGTTATTTGCATACAAGTCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTACCCTCTCCCCAAAGGGGCGAggggaccg                                                                                                                                        <  2:739839/150‑6 (MQ=255)
               ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTACCCTCTCCCCAAAGGGGCGAggggaccg                                                                                                                                        <  2:733086/150‑6 (MQ=255)
                gCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAggg                                                                                                                                       <  1:27792/150‑1 (MQ=255)
                  aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgag                                                                                                                                     <  2:1515279/150‑1 (MQ=255)
                  aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgag                                                                                                                                     <  2:1511317/150‑1 (MQ=255)
                    gTGACACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                                   <  1:1896438/150‑1 (MQ=255)
                    gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                                   >  1:309184/1‑150 (MQ=255)
                      gAAACGGTCTGTCCACTGCAATTCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAggg                                                                                                                                 <  2:1062394/150‑1 (MQ=255)
                       aaaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGTCACCTAAATTAACCACATACAGCCACCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGGCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgggg                                                                                                                                <  1:1892676/150‑1 (MQ=39)
                        aaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGATAGGGTTAGGGAGAGGGGa                                                                                                                               >  2:917623/1‑150 (MQ=255)
                           ggTCTGTCCTCTGCAATGCATATTGTTGTTTTGCATACAAGGCACCTTAATTAACGACCTACAGCCGCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGTGAGGGTTAGGGAGAGGGGaaaa                                                                                                                            <  2:914321/150‑1 (MQ=38)
                            gTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACCGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAAc                                                                                                                           <  1:467439/150‑1 (MQ=255)
                                  ccACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACAGCACCGGACGCTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAc                                                                                                                     >  2:1059408/1‑150 (MQ=255)
                                   cACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAcc                                                                                                                    >  2:1041058/1‑150 (MQ=255)
                                    aCTTCAATTAATATTGGTGATTTCCATACAAGGCACCTTATATAACTACATACAGCCTCCCGGTAGGCTGATGTAAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTTGTGAGAGGGGAAAACCCGCAccc                                                                                                                   <  2:20131/150‑1 (MQ=255)
                                         aaTGCAAATTGGTGATTTGCATACAAGGCACCTTAATTAACGACTTCCAGCCTCCCGTGAGGCTGATGTCAAACCAAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAttt                                                                                                              <  2:1344550/150‑1 (MQ=255)
                                          aTGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt                                                                                                             >  2:1771482/1‑150 (MQ=255)
                                             cATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                                                                                          >  2:1089918/1‑150 (MQ=255)
                                               tatTGGTGATTTGAATAAAAGGCACCTTAATTAACCACATACACCCTCCCGTCAGGCTTATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                                                                                        <  1:985335/150‑1 (MQ=38)
                                                 ttGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGAACCCTATTTTCAGATCa                                                                                                      >  2:553638/1‑150 (MQ=255)
                                                  tGGTGATTTGCATACAAGGCACCTTAATTAACGCCATACAGCCTCCCGTGAGGCTGATGTCACACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAg                                                                                                     >  2:323348/1‑150 (MQ=255)
                                                      gATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTAGGCGAGACGGTTAGGGAGCGGGGAAAACCCGCCCCAGATTTTCAGAGAAGTTAt                                                                                                 >  2:1667925/1‑150 (MQ=255)
                                                       ttttGCTTACAAGGCACAGTAATTAACGTCATACATGCTCCCGTGTGGATTATGTCAAACCCAAAAACTTCACCGGCCGGTCCCCTCGCCCCTTTTGGGTGTTGGTTTTGTAGAGGGGAAAACCCGCAACCTATTTTCAGATCAGTTAtt                                                                                                <  2:232988/149‑1 (MQ=255)
                                                       aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAtt                                                                                                >  1:614279/1‑150 (MQ=255)
                                                        tttGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAttt                                                                                               >  2:1799974/1‑150 (MQ=255)
                                                          tGCCTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGGAGCGGGTTAGGGAGCGGGGAAAACCCGCCCCCGATTTGCAGATCCGTTAGTTCg                                                                                             >  2:1443822/1‑150 (MQ=255)
                                                            cATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGGGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                                                                           <  2:1659085/150‑1 (MQ=255)
                                                             aTACACGGCACCTTAATTAACGACAAACAGCCTCCCGTGAGCCTGATGTCAAACCCAAAAACTGCACCTGACGGTCCCCTCGCCCCTTTGGGGAGATGGTTAGGTAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAt                                                                                          <  1:289747/150‑1 (MQ=255)
                                                                cAAGGCACCTTAATAAACGACAAACCGCCGCCCGTGTGGCTTATGTAAAACCAAAAAACTCCACCGGACGGTCCCCTCGCCCCTTTGGGGAGTGGGTTTGGTAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTaa                                                                                       <  2:561851/150‑1 (MQ=255)
                                                                  aGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCTTTAatt                                                                                     >  2:1516223/1‑149 (MQ=255)
                                                                  aGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTAAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                                                                                     <  2:5653/150‑1 (MQ=255)
                                                                      aCCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACTGTCCCCTCGCCCATTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCACCCGATGTTCAGATCAGTTATTTCGCATTAATATCca                                                                                 >  1:164716/1‑150 (MQ=255)
                                                                           aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAACACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGa                                                                            >  1:213242/1‑150 (MQ=255)
                                                                            attaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTAGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                                                                           >  1:1484336/1‑150 (MQ=255)
                                                                               aaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCg                                                                        >  2:615919/1‑150 (MQ=255)
                                                                                 cGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCt                                                                      >  2:842021/1‑150 (MQ=255)
                                                                                    caaaCAGCCACCCGTGAGGCTGATGTAAAACCAAAAAACGGCACCGGACGGTCCCCTCGCCCCTTTGGGGTGAGGGTTCGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa                                                                   <  2:708713/147‑1 (MQ=255)
                                                                                     aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCCCCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa                                                                  >  1:1561861/1‑150 (MQ=255)
                                                                                      atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGCAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGCCCCGCTGCAAc                                                                 >  1:247216/3‑150 (MQ=255)
                                                                                       aCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCAATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAccc                                                                >  2:1168358/1‑150 (MQ=255)
                                                                                       aCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAAcc                                                                >  1:78980/1‑150 (MQ=255)
                                                                                        cAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCg                                                               >  2:1485793/1‑150 (MQ=255)
                                                                                          gCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTTTTTCGCATTAATATCCAGCAGACCCGCTGCACCCGCt                                                             >  1:1090065/1‑150 (MQ=39)
                                                                                            cTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAATTCGCATTAAAATCCAGCAGCCCCGCTGCAACCGCTgg                                                           >  1:1706830/1‑150 (MQ=255)
                                                                                               ccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCACCCGCTGGAGc                                                        >  2:455769/1‑150 (MQ=255)
                                                                                                cGTGAGGCTTATGTAAAACCCAAAAACAGCACCGGACGGTCCCCTCGCCCGTTGGGGGAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGcc                                                       <  1:556187/150‑1 (MQ=255)
                                                                                                       cTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCgg                                                >  1:522593/1‑150 (MQ=255)
                                                                                                        tGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCGCCTTTGGGGAGAGGGTTAGGGATAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCggg                                               <  2:544627/150‑1 (MQ=255)
                                                                                                          aTGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGgcg                                             <  1:1053847/150‑1 (MQ=255)
                                                                                                            gTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCt                                           >  2:484135/1‑150 (MQ=255)
                                                                                                                aaCCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACg                                       >  2:1352110/1‑150 (MQ=255)
                                                                                                                  cccAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCCACCGCTGGAGCCATGCCGGGCGCTAACggg                                     >  2:902829/1‑150 (MQ=255)
                                                                                                                  cccAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACggg                                     >  2:16104/1‑150 (MQ=255)
                                                                                                                   ccAAAAACTGCACCGGCCGGTCCCCTCGCCCCTATGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATATACAGATCAGTTATTTCGCAGTAATAACCAGCAGACCCGCTGCAACCGCTGGAGCCAGTCCGGGCTCTAACgggt                                    >  2:1152935/1‑149 (MQ=255)
                                                                                                                         aCTCCACCGTACTTTCCCCTCGCCCATTTGGGTAGTGTTTTTGTGATTGGTGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATAcgcg                              <  1:917623/150‑1 (MQ=255)
                                                                                                                          cTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCAGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCgg                             >  2:1788561/1‑150 (MQ=255)
                                                                                                                           ggCACCGGCCGGTCCCCTCCCCCCTTTGGGGCGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCggg                            <  2:1706830/149‑1 (MQ=255)
                                                                                                                                    cctaacccTCTCCCCAAAGGGGCGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCCCCAgg                   >  2:103162/6‑150 (MQ=255)
                                                                                                                                    cGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGCGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAgg                   >  2:1621693/1‑150 (MQ=255)
                                                                                                                                    cGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAgg                   <  1:1240051/150‑1 (MQ=255)
                                                                                                                                       tCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGTGATCACCAGGCaa                >  2:1909699/1‑150 (MQ=255)
                                                                                                                                        ccccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAt               >  1:45715/1‑150 (MQ=255)
                                                                                                                                        acccTCTCCCCAAAGGGGCGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAt               >  1:1522147/2‑150 (MQ=255)
                                                                                                                                          ccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgc             <  2:614279/150‑1 (MQ=255)
                                                                                                                                             cGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgcagc          >  2:1910318/1‑150 (MQ=255)
                                                                                                                                               ccccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGcagcag        >  2:240604/1‑150 (MQ=255)
                                                                                                                                                cccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAATGGGATACGCGGGATCACCAGGCAATGCAGCAgg       >  2:456963/1‑150 (MQ=255)
                                                                                                                                                 ccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCTGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGt      >  1:1719630/1‑150 (MQ=255)
                                                                                                                                                 ccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGt      >  1:1425181/1‑150 (MQ=255)
                                                                                                                                                     tGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATa  >  2:1291089/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATA  >  NC_000913/4070315‑4070613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: