Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,469 A→C 6.4% intergenic (‑68/+46) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4690AC 6.4% 190.4 / 2.9 73intergenic (‑68/+46)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base A (42/23);  new base C (2/3);  total (44/29)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATA  >  NC_000913/4070320‑4070617
                                                                                                                                                     |                                                                                                                                                    
aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                                      >  2:463868/1‑150 (MQ=255)
aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                                      <  2:1075837/150‑1 (MQ=255)
   cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                                                                                   <  1:1028346/150‑1 (MQ=255)
      ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                                                                                                <  2:444738/150‑1 (MQ=255)
      ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                                                                                                <  2:451903/150‑1 (MQ=255)
       cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGtt                                                                                                                                               <  1:1591283/150‑1 (MQ=255)
          ggCAAGTGAAACGGTCTGTCCACTTCAATGCATATTGGTTATTTGCATACAAGTCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTACCCTCTCCCCAAAGGGGCGAggggaccg                                                                                                                                            <  2:739839/150‑6 (MQ=255)
          ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTACCCTCTCCCCAAAGGGGCGAggggaccg                                                                                                                                            <  2:733086/150‑6 (MQ=255)
           gCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAggg                                                                                                                                           <  1:27792/150‑1 (MQ=255)
             aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgag                                                                                                                                         <  2:1511317/150‑1 (MQ=255)
             aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgag                                                                                                                                         <  2:1515279/150‑1 (MQ=255)
               gTGACACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                                       <  1:1896438/150‑1 (MQ=255)
               gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                                       >  1:309184/1‑150 (MQ=255)
                 gAAACGGTCTGTCCACTGCAATTCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAggg                                                                                                                                     <  2:1062394/150‑1 (MQ=255)
                  aaaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGTCACCTAAATTAACCACATACAGCCACCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGGCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgggg                                                                                                                                    <  1:1892676/150‑1 (MQ=39)
                   aaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGATAGGGTTAGGGAGAGGGGa                                                                                                                                   >  2:917623/1‑150 (MQ=255)
                      ggTCTGTCCTCTGCAATGCATATTGTTGTTTTGCATACAAGGCACCTTAATTAACGACCTACAGCCGCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGTGAGGGTTAGGGAGAGGGGaaaa                                                                                                                                <  2:914321/150‑1 (MQ=38)
                       gTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACCGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAAc                                                                                                                               <  1:467439/150‑1 (MQ=255)
                             ccACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACAGCACCGGACGCTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAc                                                                                                                         >  2:1059408/1‑150 (MQ=255)
                              cACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAcc                                                                                                                        >  2:1041058/1‑150 (MQ=255)
                               aCTTCAATTAATATTGGTGATTTCCATACAAGGCACCTTATATAACTACATACAGCCTCCCGGTAGGCTGATGTAAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTTGTGAGAGGGGAAAACCCGCAccc                                                                                                                       <  2:20131/150‑1 (MQ=255)
                                    aaTGCAAATTGGTGATTTGCATACAAGGCACCTTAATTAACGACTTCCAGCCTCCCGTGAGGCTGATGTCAAACCAAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAttt                                                                                                                  <  2:1344550/150‑1 (MQ=255)
                                     aTGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt                                                                                                                 >  2:1771482/1‑150 (MQ=255)
                                        cATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                                                                                              >  2:1089918/1‑150 (MQ=255)
                                          tatTGGTGATTTGAATAAAAGGCACCTTAATTAACCACATACACCCTCCCGTCAGGCTTATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                                                                                            <  1:985335/150‑1 (MQ=38)
                                            ttGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGAACCCTATTTTCAGATCa                                                                                                          >  2:553638/1‑150 (MQ=255)
                                             tGGTGATTTGCATACAAGGCACCTTAATTAACGCCATACAGCCTCCCGTGAGGCTGATGTCACACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAg                                                                                                         >  2:323348/1‑150 (MQ=255)
                                                 gATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTAGGCGAGACGGTTAGGGAGCGGGGAAAACCCGCCCCAGATTTTCAGAGAAGTTAt                                                                                                     >  2:1667925/1‑150 (MQ=255)
                                                  ttttGCTTACAAGGCACAGTAATTAACGTCATACATGCTCCCGTGTGGATTATGTCAAACCCAAAAACTTCACCGGCCGGTCCCCTCGCCCCTTTTGGGTGTTGGTTTTGTAGAGGGGAAAACCCGCAACCTATTTTCAGATCAGTTAtt                                                                                                    <  2:232988/149‑1 (MQ=255)
                                                  aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAtt                                                                                                    >  1:614279/1‑150 (MQ=255)
                                                   tttGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAttt                                                                                                   >  2:1799974/1‑150 (MQ=255)
                                                     tGCCTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGGAGCGGGTTAGGGAGCGGGGAAAACCCGCCCCCGATTTGCAGATCCGTTAGTTCg                                                                                                 >  2:1443822/1‑150 (MQ=255)
                                                       cATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGGGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                                                                               <  2:1659085/150‑1 (MQ=255)
                                                        aTACACGGCACCTTAATTAACGACAAACAGCCTCCCGTGAGCCTGATGTCAAACCCAAAAACTGCACCTGACGGTCCCCTCGCCCCTTTGGGGAGATGGTTAGGTAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAt                                                                                              <  1:289747/150‑1 (MQ=255)
                                                           cAAGGCACCTTAATAAACGACAAACCGCCGCCCGTGTGGCTTATGTAAAACCAAAAAACTCCACCGGACGGTCCCCTCGCCCCTTTGGGGAGTGGGTTTGGTAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTaa                                                                                           <  2:561851/150‑1 (MQ=255)
                                                             aGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCTTTAatt                                                                                         >  2:1516223/1‑149 (MQ=255)
                                                             aGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTAAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                                                                                         <  2:5653/150‑1 (MQ=255)
                                                                 aCCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACTGTCCCCTCGCCCATTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCACCCGATGTTCAGATCAGTTATTTCGCATTAATATCca                                                                                     >  1:164716/1‑150 (MQ=255)
                                                                      aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAACACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGa                                                                                >  1:213242/1‑150 (MQ=255)
                                                                       attaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTAGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                                                                               >  1:1484336/1‑150 (MQ=255)
                                                                          aaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCg                                                                            >  2:615919/1‑150 (MQ=255)
                                                                            cGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCt                                                                          >  2:842021/1‑150 (MQ=255)
                                                                               caaaCAGCCACCCGTGAGGCTGATGTAAAACCAAAAAACGGCACCGGACGGTCCCCTCGCCCCTTTGGGGTGAGGGTTCGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa                                                                       <  2:708713/147‑1 (MQ=255)
                                                                                aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCCCCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa                                                                      >  1:1561861/1‑150 (MQ=255)
                                                                                 atcAGCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGCAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGCCCCGCTGCAAc                                                                     >  1:247216/3‑150 (MQ=255)
                                                                                  aCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCAATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAccc                                                                    >  2:1168358/1‑150 (MQ=255)
                                                                                  aCAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAAcc                                                                    >  1:78980/1‑150 (MQ=255)
                                                                                   cAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCg                                                                   >  2:1485793/1‑150 (MQ=255)
                                                                                     gCCTCACGGGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTTTTTCGCATTAATATCCAGCAGACCCGCTGCACCCGCt                                                                 >  1:1090065/1‑150 (MQ=39)
                                                                                       cTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAATTCGCATTAAAATCCAGCAGCCCCGCTGCAACCGCTgg                                                               >  1:1706830/1‑150 (MQ=255)
                                                                                          ccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCACCCGCTGGAGc                                                            >  2:455769/1‑150 (MQ=255)
                                                                                           cGTGAGGCTTATGTAAAACCCAAAAACAGCACCGGACGGTCCCCTCGCCCGTTGGGGGAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGcc                                                           <  1:556187/150‑1 (MQ=255)
                                                                                                  cTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCgg                                                    >  1:522593/1‑150 (MQ=255)
                                                                                                   tGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCGCCTTTGGGGAGAGGGTTAGGGATAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCggg                                                   <  2:544627/150‑1 (MQ=255)
                                                                                                     aTGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGgcg                                                 <  1:1053847/150‑1 (MQ=255)
                                                                                                       gTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCt                                               >  2:484135/1‑150 (MQ=255)
                                                                                                           aaCCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACg                                           >  2:1352110/1‑150 (MQ=255)
                                                                                                             cccAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCCACCGCTGGAGCCATGCCGGGCGCTAACggg                                         >  2:902829/1‑150 (MQ=255)
                                                                                                             cccAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACggg                                         >  2:16104/1‑150 (MQ=255)
                                                                                                              ccAAAAACTGCACCGGCCGGTCCCCTCGCCCCTATGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATATACAGATCAGTTATTTCGCAGTAATAACCAGCAGACCCGCTGCAACCGCTGGAGCCAGTCCGGGCTCTAACgggt                                        >  2:1152935/1‑149 (MQ=255)
                                                                                                                    aCTCCACCGTACTTTCCCCTCGCCCATTTGGGTAGTGTTTTTGTGATTGGTGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATAcgcg                                  <  1:917623/150‑1 (MQ=255)
                                                                                                                     cTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCAGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCgg                                 >  2:1788561/1‑150 (MQ=255)
                                                                                                                      ggCACCGGCCGGTCCCCTCCCCCCTTTGGGGCGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCggg                                <  2:1706830/149‑1 (MQ=255)
                                                                                                                               cctaacccTCTCCCCAAAGGGGCGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCCCCAgg                       >  2:103162/6‑150 (MQ=255)
                                                                                                                               cGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGCGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAgg                       >  2:1621693/1‑150 (MQ=255)
                                                                                                                               cGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAgg                       <  1:1240051/150‑1 (MQ=255)
                                                                                                                                  tCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGTGATCACCAGGCaa                    >  2:1909699/1‑150 (MQ=255)
                                                                                                                                   ccccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAt                   >  1:45715/1‑150 (MQ=255)
                                                                                                                                   acccTCTCCCCAAAGGGGCGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAAt                   >  1:1522147/2‑150 (MQ=255)
                                                                                                                                     ccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgc                 <  2:614279/150‑1 (MQ=255)
                                                                                                                                        cGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgcagc              >  2:1910318/1‑150 (MQ=255)
                                                                                                                                          ccccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGcagcag            >  2:240604/1‑150 (MQ=255)
                                                                                                                                           cccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAATGGGATACGCGGGATCACCAGGCAATGCAGCAgg           >  2:456963/1‑150 (MQ=255)
                                                                                                                                            ccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCTGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGt          >  1:1719630/1‑150 (MQ=255)
                                                                                                                                            ccTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGt          >  1:1425181/1‑150 (MQ=255)
                                                                                                                                                tGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATa      >  2:1291089/1‑150 (MQ=255)
                                                                                                                                                    gagaGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGATATCGGGAGACGCGGGATCACCAGGCAATGCAGCAGGTGATAAata  >  1:1159162/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATA  >  NC_000913/4070320‑4070617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: