Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,277,225 G→A 5.2% Y53Y (TAC→TAT) ‡ soxS ← superoxide response regulon transcriptional activator; autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,2250GA 5.2% 436.1 / 8.3 136Y53Y (TAC→TAT) ‡soxSsuperoxide response regulon transcriptional activator; autoregulator
Reads supporting (aligned to +/- strand):  ref base G (68/60);  new base A (5/2);  total (73/63)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 138 total aligned reads displayed.

TAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTC  >  NC_000913/4277076‑4277372
                                                                                                                                                     |                                                                                                                                                   
tAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATg                                                                                                                                                     <  1:1135169/150‑1 (MQ=255)
tAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATg                                                                                                                                                     <  1:468453/150‑1 (MQ=255)
 aaTCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGt                                                                                                                                                    >  1:832033/1‑150 (MQ=255)
 aaTCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGt                                                                                                                                                    <  1:689345/150‑1 (MQ=255)
  aTCGCTGGGAGTGCGATCAAACTGCCGACGGAACACGCGGGAGAAGGTCTGCTGCGAGCCCTAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTa                                                                                                                                                   >  1:147687/1‑150 (MQ=255)
   tCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCACTAACAGGCGGCGTTGGCGAATGTaa                                                                                                                                                  >  1:915235/1‑150 (MQ=255)
      cTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCg                                                                                                                                               <  2:1254151/150‑1 (MQ=255)
      cTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCg                                                                                                                                               <  2:1235666/150‑1 (MQ=255)
           cgTGCGATCAAACTGCCGACGGAAAACGCGTGAGAAGGTCTGCTGCGATACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAg                                                                                                                                          <  1:322740/149‑1 (MQ=255)
           aGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAg                                                                                                                                          <  2:33559/150‑1 (MQ=255)
             tGCGAGCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACATTCGCCaacgc                                                                                                                                        <  1:819352/150‑4 (MQ=255)
              gCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCACCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGctt                                                                                                                                       >  1:758563/1‑148 (MQ=255)
              gCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCAATGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGt                                                                                                                                       >  1:1302220/1‑150 (MQ=255)
                gATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCt                                                                                                                                     >  2:973789/1‑150 (MQ=255)
                         gCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTAAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCgacc                                                                                                                            >  1:459424/1‑147 (MQ=255)
                         gCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGATCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCa                                                                                                                            <  2:138474/150‑1 (MQ=255)
                          ccGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAc                                                                                                                           >  1:124616/1‑150 (MQ=255)
                            gACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCg                                                                                                                         >  1:1868219/1‑150 (MQ=255)
                               ggAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGc                                                                                                                      >  1:1744141/1‑150 (MQ=255)
                               ggAAAACGCGGGAGAAGGGCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGc                                                                                                                      <  2:1851032/150‑1 (MQ=255)
                                gAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGAGATATCAAAAATCGTACGCTCGGTGGTGCGCAACTCAACGCCGGCCCGTAACAGGCGGCGTTCGCAAATGTAAACGCCAAGCGACTGATGCGTCCACGTGCg                                                                                                                     >  2:966858/1‑150 (MQ=255)
                                 aaaaCGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCgg                                                                                                                    <  2:58764/150‑1 (MQ=255)
                                 aaaaCGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCgg                                                                                                                    >  1:1288516/1‑150 (MQ=255)
                                    aCGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAc                                                                                                                 >  1:406663/1‑150 (MQ=255)
                                      gcgGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACAt                                                                                                               <  2:1630933/150‑1 (MQ=255)
                                          gagaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGt                                                                                                           <  1:505706/150‑1 (MQ=255)
                                                   tgctgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCGACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAc                                                                                                  >  2:379726/1‑150 (MQ=255)
                                                   tgctgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAc                                                                                                  >  2:42416/1‑150 (MQ=255)
                                                     ctgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCa                                                                                                <  2:268255/150‑1 (MQ=255)
                                                      tgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCAc                                                                                               >  1:1145437/1‑150 (MQ=255)
                                                       gcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCAGATGCGTCACCGTACGGAACATTCGTAGCAAGTACCACt                                                                                              >  1:293580/1‑150 (MQ=255)
                                                       gcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACt                                                                                              >  1:1031890/1‑150 (MQ=255)
                                                        cGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACtt                                                                                             >  2:1381941/1‑150 (MQ=255)
                                                           gaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAcc                                                                                                 >  2:356844/1‑143 (MQ=255)
                                                           gaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAcc                                                                                                 <  1:356844/143‑1 (MQ=255)
                                                           gaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGa                                                                                          >  1:1753019/1‑150 (MQ=255)
                                                             cATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAAt                                                                                        <  2:1516056/150‑1 (MQ=255)
                                                             cATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAAt                                                                                        >  2:1898951/1‑150 (MQ=255)
                                                               aaaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAg                                                                                      <  1:825056/149‑1 (MQ=255)
                                                                aaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGc                                                                                     <  1:130416/150‑1 (MQ=255)
                                                                  cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                   <  1:1764765/150‑1 (MQ=255)
                                                                  cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                   <  2:432192/150‑1 (MQ=255)
                                                                  cccAGGTCCATTGCGATATCAAAAATCGGACCCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAAAAGGCGGCGTTCGCGAATGTACTCGCCAAGCGTCTGAGGCGTCACCGAGCGGAACAATCGTTGCAAGTACCACTTTGAATAGCCt                                                                                   >  1:1547551/1‑150 (MQ=255)
                                                                   ccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTg                                                                                  <  2:1340415/150‑1 (MQ=255)
                                                                      ggTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAtt                                                                               >  2:1732777/1‑150 (MQ=255)
                                                                       gTCCATTGCGATATCAAAACTCGGCCGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                              <  2:1553405/150‑1 (MQ=255)
                                                                       gTCCAATGCGATCTCAAACATCGGACGCGCGGGGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGGCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                              <  2:909697/150‑1 (MQ=255)
                                                                            ttGCGATATCAAAATTCGGACTCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACATGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTAGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTg                                                                         <  1:1407920/150‑1 (MQ=255)
                                                                            ttGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTg                                                                         <  1:418175/150‑1 (MQ=255)
                                                                             tGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                        <  1:794931/150‑1 (MQ=255)
                                                                              gCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCg                                                                       <  2:589455/150‑1 (MQ=255)
                                                                                gATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGAc                                                                     <  1:1696282/150‑1 (MQ=255)
                                                                                 atatCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACACGCGGCGTTGGCGAATGTAAACGCCAAGCGTCTGATGCGTCACCGTGCGGCACATTCCTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACt                                                                    >  1:206946/1‑150 (MQ=255)
                                                                                  tatCAAAAATCGGACGCTCGGGGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTa                                                                   <  1:404743/150‑1 (MQ=255)
                                                                                    acAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTACTCGCCACGCGTCTGATGCGTCAACGTGCGGAACATTCGTTGCGAGTACCACTTTGAATAGCCTGAAGTTTTTGCGCCTAcc                                                                 >  1:1147498/2‑149 (MQ=255)
                                                                                     cAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTACTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGCCTACAt                                                                >  2:743732/1‑150 (MQ=255)
                                                                                     cAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACAt                                                                >  1:578162/1‑150 (MQ=255)
                                                                                     cAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACAt                                                                >  2:1316973/1‑150 (MQ=255)
                                                                                       aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCa                                                              >  1:927348/1‑150 (MQ=255)
                                                                                       aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCa                                                              <  2:19654/150‑1 (MQ=255)
                                                                                            cGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGtt                                                         >  2:1647713/1‑150 (MQ=255)
                                                                                             ggACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTa                                                        >  1:1676978/1‑150 (MQ=255)
                                                                                                cGCTCGGTGGTGCGCTACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                     <  2:1837244/150‑1 (MQ=255)
                                                                                                cGCTCGGTGGTGCGCATCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCTTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                     <  2:1361941/150‑1 (MQ=255)
                                                                                                 gCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCg                                                    >  1:1791481/1‑150 (MQ=255)
                                                                                                  cTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCgg                                                   >  2:673200/1‑150 (MQ=255)
                                                                                                        ggtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTc                                             <  1:1642249/150‑1 (MQ=255)
                                                                                                            ctctaCTCAACGGCGGCCAGTTAGAGGCTGCGTTGGCGAATGTATTCGCCAAGCGTCTGATGCGTCACCGTTCTGAACATTCGTTGCAAGTACCACTTTGAATATCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAata                                         <  1:276738/146‑1 (MQ=255)
                                                                                                            cgcTACTCAACGGCGGCCTGTAACAGGAGGCGTTGGCGAATTTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAGATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACCTCAATGTTAAGCGGCTGGTCAata                                         <  2:52427/150‑1 (MQ=255)
                                                                                                             gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                        <  1:71153/150‑1 (MQ=255)
                                                                                                             gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                        >  2:1644727/1‑150 (MQ=255)
                                                                                                                aCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                     <  2:1760039/150‑1 (MQ=255)
                                                                                                                  tCAACGGCGGCCAGTAACCGGCGGCGTTGGCGAATGTAAGCGCCAAGCGTCTGATGCGGCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCg                                   <  2:1144522/150‑1 (MQ=255)
                                                                                                                  tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCg                                   >  1:1379999/1‑150 (MQ=255)
                                                                                                                  tCAACGGCGGCCAGTAACAGGCGGCGTTGGAGAATGTAATCGCCAAGAGTATGATGAGTCACCGTGCGGAACATTAGTTGCAAGTTCGACTTTGAATATCCTTATTTTTTTGCGACTTCATCAATGTTAAGCGGCTGGGCAATATGCTCg                                   >  2:270086/1‑150 (MQ=255)
                                                                                                                   cAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGt                                  >  1:1378558/1‑150 (MQ=255)
                                                                                                                    aaCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTc                                 <  1:332862/150‑1 (MQ=255)
                                                                                                                    aaCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTc                                 <  2:1562931/150‑1 (MQ=255)
                                                                                                                      cggcggCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCaa                               <  2:1906736/150‑1 (MQ=255)
                                                                                                                          ggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCa                           <  1:1105720/150‑1 (MQ=255)
                                                                                                                           gcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTAGTCAATCCAt                          >  1:1174176/1‑150 (MQ=255)
                                                                                                                               gTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGCACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGa                      >  1:1601997/1‑150 (MQ=255)
                                                                                                                               gTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGa                      >  2:1294528/1‑150 (MQ=255)
                                                                                                                                tAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGAt                     <  2:1302220/150‑1 (MQ=255)
                                                                                                                                tAACAGGCGGCATTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATGCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGAt                     <  2:103498/150‑1 (MQ=255)
                                                                                                                                 aaCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATa                    >  2:54448/1‑150 (MQ=255)
                                                                                                                                     ggcggcGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGGGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCTTCCGATAAGAt                >  2:173092/1‑150 (MQ=255)
                                                                                                                                     ggcggcGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGAt                >  1:658346/1‑150 (MQ=255)
                                                                                                                                        ggcgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAACGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTAGTCAATCCATGCGATAAGATCCt             >  1:142357/1‑150 (MQ=255)
                                                                                                                                        acgcttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCt             >  1:1716493/5‑150 (MQ=255)
                                                                                                                                          cgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGa           >  2:497554/1‑150 (MQ=255)
                                                                                                                                           cttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaa          >  1:977047/2‑150 (MQ=255)
                                                                                                                                           cttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaa          >  1:1534353/2‑150 (MQ=255)
                                                                                                                                            ttGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaat         <  2:124616/150‑1 (MQ=255)
                                                                                                                                             tGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaata        >  2:739093/1‑150 (MQ=255)
                                                                                                                                              ggCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaataa       >  2:864943/1‑150 (MQ=255)
                                                                                                                                                cGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGCCTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAttt     >  1:1662056/1‑150 (MQ=255)
                                                                                                                                                  aaTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtttt   <  2:799244/150‑1 (MQ=255)
                                                                                                                                                   aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTc  <  1:1111568/150‑1 (MQ=255)
                                                                                                                                                   aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTc  >  1:599878/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTC  >  NC_000913/4277076‑4277372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: