Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,277,226 T→C 5.2% Y53C (TAC→TGC) ‡ soxS ← superoxide response regulon transcriptional activator; autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,2260TC 5.2% 416.1 / 8.9 134Y53C (TAC→TGC) ‡soxSsuperoxide response regulon transcriptional activator; autoregulator
Reads supporting (aligned to +/- strand):  ref base T (67/60);  new base C (5/2);  total (72/62)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 139 total aligned reads displayed.

AATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTCTGA  >  NC_000913/4277077‑4277375
                                                                                                                                                     |                                                                                                                                                     
aaTCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGt                                                                                                                                                       >  1:832033/1‑150 (MQ=255)
aaTCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGt                                                                                                                                                       <  1:689345/150‑1 (MQ=255)
 aTCGCTGGGAGTGCGATCAAACTGCCGACGGAACACGCGGGAGAAGGTCTGCTGCGAGCCCTAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTa                                                                                                                                                      >  1:147687/1‑150 (MQ=255)
  tCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCACTAACAGGCGGCGTTGGCGAATGTaa                                                                                                                                                     >  1:915235/1‑150 (MQ=255)
       gggAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGcc                                                                                                                                                <  2:31703/150‑1 (MQ=255)
          cgTGCGATCAAACTGCCGACGGAAAACGCGTGAGAAGGTCTGCTGCGATACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAg                                                                                                                                             <  1:322740/149‑1 (MQ=255)
            tGCGAGCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACATTCGCCaacgc                                                                                                                                           <  1:819352/150‑4 (MQ=255)
             gCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCACCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGctt                                                                                                                                          >  1:758563/1‑148 (MQ=255)
             gCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCAATGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGt                                                                                                                                          >  1:1302220/1‑150 (MQ=255)
               gATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCt                                                                                                                                        >  2:973789/1‑150 (MQ=255)
                   aaaCTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATg                                                                                                                                    <  1:367143/150‑1 (MQ=255)
                        gCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTAAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCgacc                                                                                                                               >  1:459424/1‑147 (MQ=255)
                        gCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGATCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCa                                                                                                                               <  2:138474/150‑1 (MQ=255)
                         ccGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAc                                                                                                                              >  1:124616/1‑150 (MQ=255)
                           gACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCg                                                                                                                            >  1:1868219/1‑150 (MQ=255)
                              ggAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTTGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGc                                                                                                                         <  1:1249736/150‑1 (MQ=255)
                              ggAAAACGCGGGAGAAGGGCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGc                                                                                                                         <  2:1851032/150‑1 (MQ=255)
                               gAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGAGATATCAAAAATCGTACGCTCGGTGGTGCGCAACTCAACGCCGGCCCGTAACAGGCGGCGTTCGCAAATGTAAACGCCAAGCGACTGATGCGTCCACGTGCg                                                                                                                        >  2:966858/1‑150 (MQ=255)
                                aaaaCGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCgg                                                                                                                       <  2:58764/150‑1 (MQ=255)
                                         gagaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGt                                                                                                              <  1:505706/150‑1 (MQ=255)
                                           gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTg                                                                                                            <  1:1378392/150‑1 (MQ=255)
                                              ggTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGGGGACCATTCGTTGCaa                                                                                                         >  1:1163845/1‑150 (MQ=255)
                                                  tgctgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCGACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAc                                                                                                     >  2:379726/1‑150 (MQ=255)
                                                  tgctgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAc                                                                                                     >  2:42416/1‑150 (MQ=255)
                                                    ctgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCa                                                                                                   <  2:268255/150‑1 (MQ=255)
                                                     tgcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCAc                                                                                                  >  1:1145437/1‑150 (MQ=255)
                                                      gcGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACt                                                                                                 >  1:1031890/1‑150 (MQ=255)
                                                          gaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTAcc                                                                                                    >  2:356844/1‑143 (MQ=255)
                                                          gaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGa                                                                                             >  1:1753019/1‑150 (MQ=255)
                                                            cATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAAt                                                                                           >  2:1898951/1‑150 (MQ=255)
                                                              tAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCACCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAg                                                                                         >  1:1494830/1‑150 (MQ=255)
                                                              aaaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAg                                                                                         <  1:825056/149‑1 (MQ=255)
                                                               aaCCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGc                                                                                        <  1:130416/150‑1 (MQ=255)
                                                                 cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                      <  1:1764765/150‑1 (MQ=255)
                                                                 cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCt                                                                                      <  2:432192/150‑1 (MQ=255)
                                                                 cccAGGTCCATTGCGATATCAAAAATCGGACCCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAAAAGGCGGCGTTCGCGAATGTACTCGCCAAGCGTCTGAGGCGTCACCGAGCGGAACAATCGTTGCAAGTACCACTTTGAATAGCCt                                                                                      >  1:1547551/1‑150 (MQ=255)
                                                                  ccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTg                                                                                     <  2:1340415/150‑1 (MQ=255)
                                                                     ggTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAtt                                                                                  >  2:1732777/1‑150 (MQ=255)
                                                                      gTCCATTGCGATATCAAAACTCGGCCGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                                 <  2:1553405/150‑1 (MQ=255)
                                                                      gTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGCTGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                                 >  1:306000/1‑150 (MQ=255)
                                                                      gTCCAATGCGATCTCAAACATCGGACGCGCGGGGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGGCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAttt                                                                                 <  2:909697/150‑1 (MQ=255)
                                                                           ttGCGATATCAAAATTCGGACTCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACATGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTAGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTg                                                                            <  1:1407920/150‑1 (MQ=255)
                                                                           ttGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTg                                                                            <  1:418175/150‑1 (MQ=255)
                                                                            tGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                           <  1:973789/150‑1 (MQ=255)
                                                                            tGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGc                                                                           <  1:794931/150‑1 (MQ=255)
                                                                             gCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCg                                                                          <  2:589455/150‑1 (MQ=255)
                                                                               gATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGAc                                                                        <  1:1696282/150‑1 (MQ=255)
                                                                                atatCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACACGCGGCGTTGGCGAATGTAAACGCCAAGCGTCTGATGCGTCACCGTGCGGCACATTCCTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACt                                                                       >  1:206946/1‑150 (MQ=255)
                                                                                 tatCAAAAATCGGACGCTCGGGGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTa                                                                      <  1:404743/150‑1 (MQ=255)
                                                                                   acAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTACTCGCCACGCGTCTGATGCGTCAACGTGCGGAACATTCGTTGCGAGTACCACTTTGAATAGCCTGAAGTTTTTGCGCCTAcc                                                                    >  1:1147498/2‑149 (MQ=255)
                                                                                    cAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTACTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGCCTACAt                                                                   >  2:743732/1‑150 (MQ=255)
                                                                                    cAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACaa                                                                   >  2:1505322/1‑149 (MQ=255)
                                                                                    cAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACAt                                                                   >  1:578162/1‑150 (MQ=255)
                                                                                           cGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGtt                                                            >  2:1647713/1‑150 (MQ=255)
                                                                                            ggACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAGCATTCGTTGCAAGTACCACTTTGAATAGCCTGATGTTTTTGCGAGTACATCATGGTTa                                                           >  2:1156963/1‑150 (MQ=255)
                                                                                            ggACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTa                                                           >  1:1676978/1‑150 (MQ=255)
                                                                                            ggACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTa                                                           >  1:41763/1‑150 (MQ=255)
                                                                                               cGCTCGGTGGTGCGCTACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                        <  2:1837244/150‑1 (MQ=255)
                                                                                               cGCTCGGTGGTGCGCATCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCTTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                        <  2:1361941/150‑1 (MQ=255)
                                                                                                gCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCg                                                       >  1:1791481/1‑150 (MQ=255)
                                                                                                 cTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCgg                                                      >  2:673200/1‑150 (MQ=255)
                                                                                                       ggtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTc                                                <  1:1642249/150‑1 (MQ=255)
                                                                                                           ctctaCTCAACGGCGGCCAGTTAGAGGCTGCGTTGGCGAATGTATTCGCCAAGCGTCTGATGCGTCACCGTTCTGAACATTCGTTGCAAGTACCACTTTGAATATCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAata                                            <  1:276738/146‑1 (MQ=255)
                                                                                                           cgcTACTCAACGGCGGCCTGTAACAGGAGGCGTTGGCGAATTTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAAGATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACCTCAATGTTAAGCGGCTGGTCAata                                            <  2:52427/150‑1 (MQ=255)
                                                                                                            gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                           <  1:71153/150‑1 (MQ=255)
                                                                                                            gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                           >  2:1644727/1‑150 (MQ=255)
                                                                                                               aCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCt                                        >  1:1871194/1‑150 (MQ=255)
                                                                                                                 tCAACGGCGGCCAGTAACCGGCGGCGTTGGCGAATGTAAGCGCCAAGCGTCTGATGCGGCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCg                                      <  2:1144522/150‑1 (MQ=255)
                                                                                                                 tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCg                                      >  1:1379999/1‑150 (MQ=255)
                                                                                                                 tCAACGGCGGCCAGTAACAGGCGGCGTTGGAGAATGTAATCGCCAAGAGTATGATGAGTCACCGTGCGGAACATTAGTTGCAAGTTCGACTTTGAATATCCTTATTTTTTTGCGACTTCATCAATGTTAAGCGGCTGGGCAATATGCTCg                                      >  2:270086/1‑150 (MQ=255)
                                                                                                                  cAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGt                                     >  1:1734098/1‑150 (MQ=255)
                                                                                                                   aaCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTc                                    <  2:486612/150‑1 (MQ=255)
                                                                                                                   aaCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTc                                    <  2:1562931/150‑1 (MQ=255)
                                                                                                                   aaCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTc                                    <  1:332862/150‑1 (MQ=255)
                                                                                                                   aaCGGCGGCCAGTAACAGGCGGCGGTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAGCGTGCGGAACATTCGTTGCAAGTCCCACTTAGAATAGCCTGATTTTTTTGCGACTACATCATTGTTAAGCGGCTGGTCAATATGCTCGTc                                    <  2:191793/150‑1 (MQ=255)
                                                                                                                     cggcggCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCaa                                  <  2:1906736/150‑1 (MQ=255)
                                                                                                                         ggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCa                              <  1:1105720/150‑1 (MQ=255)
                                                                                                                           ccAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                            >  2:1247477/1‑150 (MQ=255)
                                                                                                                           ccAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                            <  2:1744141/150‑1 (MQ=255)
                                                                                                                             aGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCg                          <  2:1379896/150‑1 (MQ=255)
                                                                                                                              gTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGCACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGa                         >  1:1601997/1‑150 (MQ=255)
                                                                                                                              gTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGa                         >  2:1294528/1‑150 (MQ=255)
                                                                                                                               tAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGAt                        <  2:1302220/150‑1 (MQ=255)
                                                                                                                               tAACAGGCGGCATTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATGCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGAt                        <  2:103498/150‑1 (MQ=255)
                                                                                                                                aaCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATa                       >  2:54448/1‑150 (MQ=255)
                                                                                                                                  cAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAg                     >  2:1311958/1‑150 (MQ=255)
                                                                                                                                    ggcggcGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGGGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCTTCCGATAAGAt                   >  2:173092/1‑150 (MQ=255)
                                                                                                                                    ggcggcGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGAt                   >  2:1617025/1‑150 (MQ=255)
                                                                                                                                    ggcggcGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGAt                   >  1:658346/1‑150 (MQ=255)
                                                                                                                                       ggcgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAACGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTAGTCAATCCATGCGATAAGATCCt                >  1:142357/1‑150 (MQ=255)
                                                                                                                                       acgcttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCt                >  1:1716493/5‑150 (MQ=255)
                                                                                                                                           ttGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaat            <  2:124616/150‑1 (MQ=255)
                                                                                                                                            tGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaata           >  2:739093/1‑150 (MQ=255)
                                                                                                                                             ggCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaataa          >  2:864943/1‑150 (MQ=255)
                                                                                                                                               cGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGCCTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAttt        >  1:1662056/1‑150 (MQ=255)
                                                                                                                                                 aaTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtttt      <  2:799244/150‑1 (MQ=255)
                                                                                                                                                  aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTc     <  1:1111568/150‑1 (MQ=255)
                                                                                                                                                  aTGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTc     >  1:599878/1‑150 (MQ=255)
                                                                                                                                                     tAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAAGCCATGCGATAAGATCCTGAATAATTTTCTGa  >  1:947869/1‑150 (MQ=255)
                                                                                                                                                     tAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACAGTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATAAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTCTGa  >  1:904609/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATTTTCTGA  >  NC_000913/4277077‑4277375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: