Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,283,228 T→G 6.7% intergenic (‑153/+25) yjcF ← / ← actP pentapeptide repeats protein/acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,283,2280TG 6.7% 240.9 / 2.0 117intergenic (‑153/+25)yjcF/actPpentapeptide repeats protein/acetate transporter
Reads supporting (aligned to +/- strand):  ref base T (55/52);  new base G (3/7);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGA  >  NC_000913/4283084‑4283377
                                                                                                                                                |                                                                                                                                                     
cTTATAATTAACAAGAAAGAAAATGCTTATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                  <  2:272104/150‑1 (MQ=255)
  tataATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgacga                                                                                                                                                >  1:500339/1‑150 (MQ=255)
     aaTTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCg                                                                                                                                             >  2:1250078/1‑150 (MQ=255)
        tAACAAGAAAGAAAAGGCATTTTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAATGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCgggg                                                                                                                                          <  2:1499283/150‑1 (MQ=255)
        tAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCgggg                                                                                                                                          <  2:1191644/150‑1 (MQ=255)
        tAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCgggg                                                                                                                                          <  2:774376/150‑1 (MQ=255)
        tAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCgggg                                                                                                                                          <  2:857627/150‑1 (MQ=255)
          aCAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGaa                                                                                                                                        >  1:289891/1‑150 (MQ=255)
           cAAGAAAGAAAAGGGATATTATCTGCATTTTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACTACCGGGGAAg                                                                                                                                       >  2:973645/1‑150 (MQ=255)
           cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAg                                                                                                                                       >  1:1158556/1‑150 (MQ=255)
             agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAggg                                                                                                                                     <  1:448051/150‑1 (MQ=255)
              caaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgggg                                                                                                                                    <  1:874450/149‑1 (MQ=255)
               aaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGga                                                                                                                                   >  2:298767/1‑150 (MQ=255)
                aagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgag                                                                                                                                  >  2:433395/1‑150 (MQ=255)
                 agaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgaga                                                                                                                                 >  2:818653/1‑150 (MQ=255)
                 agaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgaga                                                                                                                                 >  2:1138494/1‑150 (MQ=255)
                 agaaaAGGCATATTCTCTGCATTATCATTGTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGCTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgaga                                                                                                                                 <  1:282805/150‑1 (MQ=255)
                      aGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTa                                                                                                                            >  2:430485/1‑150 (MQ=255)
                       ggCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTaa                                                                                                                           >  2:435416/1‑150 (MQ=255)
                       ggCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTAAGACGACCGGGGAAGGGGAGAGATTaa                                                                                                                           >  2:484652/1‑150 (MQ=255)
                       gccATATTCTCTGCAATATCATTTTCTACGACTGCCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTaa                                                                                                                           <  1:607275/148‑1 (MQ=255)
                         cATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGTGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACGACCGGGGAAGGGGAGAGAATAATg                                                                                                                         >  2:936422/1‑150 (MQ=255)
                         cATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATg                                                                                                                         >  2:622157/1‑150 (MQ=255)
                               ctctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGcgcgcg                                                                                                                   <  1:839258/150‑1 (MQ=255)
                                   gCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCt                                                                                                               <  2:735250/150‑1 (MQ=255)
                                        aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAATGGGTGCGATTAATGCGCCCGGCCTTGCTc                                                                                                          >  1:960286/1‑150 (MQ=255)
                                        aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGATGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCGGCCTTGCta                                                                                                          >  1:1817460/1‑149 (MQ=255)
                                        aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTGTATTTGTTTAATATTATGGGAAAAGGTTATGCATTTGGGAGATGAACAGTATTCCACCTTCAGACCACCCGGGATGGGTAGAGATTAATTCGCGCCGCCTTGcag                                                                                                          >  2:830743/1‑148 (MQ=255)
                                         tCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGATAGGAAGAGTATTCCCCGGTCAGAAGACCGGGGAAGGGGAGAGATTAATTCTCGCGGCCTTTCTCa                                                                                                         >  1:1211078/1‑150 (MQ=255)
                                         tCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCTGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCa                                                                                                         >  1:1284290/1‑150 (MQ=255)
                                         tCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCa                                                                                                         >  2:718667/1‑150 (MQ=255)
                                           aTTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                       >  1:1772558/1‑150 (MQ=255)
                                           aTTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCCTCAGACCACCGGGGAAGGGGTGAGATTAATGCGCGCGGCCATGCTCaaa                                                                                                       >  2:634619/1‑149 (MQ=255)
                                                cTAAGACTTTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCaa                                                                                                  <  1:609125/150‑1 (MQ=255)
                                                  aCGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTAAGACGACCGGGGAAGGGGAGAGATTAATGAGCGCGGCCATGCTAAAAGCCaaaa                                                                                                >  1:1394681/1‑149 (MQ=255)
                                                   cGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCACGGTCAGACGAACGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTTCTCAACGCCacacc                                                                                               >  1:510670/1‑146 (MQ=255)
                                                      cTGTCAAAAATCGCACATATTTTAATGAGTTTATTTGTTAAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCgg                                                                                            <  1:72104/150‑1 (MQ=255)
                                                      cTGTAAAAACTCGCTAATTTTTTAATGAGTTTATTTGTTTATTATAATGGGAAAAGCTGTTGCAGTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGcctg                                                                                            <  2:18500/150‑3 (MQ=255)
                                                        gTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTc                                                                                          >  2:965122/1‑150 (MQ=255)
                                                             aaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCACCGCCAAAGCCGGTCTgggt                                                                                     >  1:907161/1‑149 (MQ=255)
                                                              aaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAg                                                                                    <  1:468513/150‑1 (MQ=255)
                                                                tCGCTCATTTTTTAATGTGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTAATCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  <  1:425950/150‑1 (MQ=255)
                                                                tCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  <  1:965122/150‑1 (MQ=255)
                                                                tCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  <  1:187225/150‑1 (MQ=255)
                                                                ccGCTCATTTTTAAATGAGTTTATTTTTTTAATATTATGGGAAAAGGTGATGCATTTGGGCTCGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  <  2:955740/149‑1 (MQ=255)
                                                                acGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCTGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  <  2:454737/149‑1 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                 <  2:289706/150‑1 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                 <  2:712269/150‑1 (MQ=255)
                                                                  gCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGa                                                                                <  1:1262564/150‑1 (MQ=255)
                                                                  gCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGa                                                                                <  2:1844598/150‑1 (MQ=255)
                                                                   cTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGCGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGAt                                                                               <  1:1185189/150‑1 (MQ=255)
                                                                   cTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGAt                                                                               <  1:101034/150‑1 (MQ=255)
                                                                    tCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATa                                                                              <  1:1237301/150‑1 (MQ=255)
                                                                    tCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCAGGTCAGACGAACGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATa                                                                              >  1:1044921/1‑150 (MQ=255)
                                                                     cATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCATAGCCGGTCTGGGAGCGGATaa                                                                             >  1:216739/1‑150 (MQ=255)
                                                                     cATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaa                                                                             >  2:1065861/1‑150 (MQ=255)
                                                                       ttttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAAc                                                                           <  2:1337608/150‑1 (MQ=255)
                                                                        tttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGTGAGATTAATTCGCGCGGCCATGCTCAACCCCAAAGCCGGTCTGGGAGCGGATaaaaa                                                                          >  2:884466/1‑148 (MQ=255)
                                                                            tAATGAGTTTATTCGGTTACTTTTATGGGAACCGGGCATGCAGGTGGGAGAGGACGAGGATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgcgc                                                                      <  2:946163/150‑1 (MQ=255)
                                                                             aaTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgcgcg                                                                     <  1:430485/150‑1 (MQ=255)
                                                                              aagAGTTTATTTGTTTAATATTATGGCAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCCGCCGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAATGCCGGTCTGGGAGTGGATAAACTgcgcgc                                                                    <  1:936422/148‑1 (MQ=255)
                                                                               tGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGcgcgcg                                                                   >  1:1546690/1‑150 (MQ=255)
                                                                               tGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGcgcgcg                                                                   <  1:1551395/150‑1 (MQ=255)
                                                                                  gTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACAGCGCGCGGaa                                                                >  1:1110242/1‑150 (MQ=255)
                                                                                      aTTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGt                                                            >  2:645297/1‑150 (MQ=255)
                                                                                          gTTTAATATTAAGGCAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcac                                                        <  1:1662279/150‑1 (MQ=255)
                                                                                               atatTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGTGTATTCCCCGGGCAGCCGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctca                                                   <  1:298767/150‑1 (MQ=255)
                                                                                               atatTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAATGGGTGAGATTAATGCGCGCGGCCATGCTCAACGCCAAAGCCGGTCTGGGTGCGGATAAACTTCGCGCGGAACAGTtaccgctca                                                   >  2:1656643/1‑150 (MQ=255)
                                                                                                tattatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctccc                                                  >  2:1290619/1‑148 (MQ=255)
                                                                                                tattatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctcac                                                  >  2:453406/1‑150 (MQ=255)
                                                                                                   tatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGCCCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCCACGCCAAAGCCGGTCTGGGGGCGGCTAAACTGCGCGGGGAACAGTTAACGCTCCcgcg                                               >  2:1405136/1‑150 (MQ=255)
                                                                                                     tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcgcg                                             <  1:1239007/150‑1 (MQ=255)
                                                                                                      gggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCCCGCTCACgcgcgc                                            >  2:1795570/1‑150 (MQ=255)
                                                                                                      gggAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACgcgcgc                                            <  2:555128/150‑1 (MQ=255)
                                                                                                       ggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGAACGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGTATAAACTGCGCGCGGAACAGTTCAAGCTCACGCGCGcc                                           >  2:1120812/1‑150 (MQ=255)
                                                                                                          aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGCCCGGGGAAGGGGAGAGATTAATTCGCGCGGCCATGCTCAACGCCAAAGCCGGTGTGGGGGCGGATAAACTGCGCGCGGCACAGTTCACGCTCCCCCGCGCCttg                                        >  1:1571565/1‑149 (MQ=255)
                                                                                                           aaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTcc                                       <  2:500339/150‑1 (MQ=255)
                                                                                                             ggTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGc                                     <  2:200599/150‑1 (MQ=255)
                                                                                                             ggTGAGGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGCCCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGc                                     <  1:1699762/150‑1 (MQ=255)
                                                                                                              gTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCt                                    <  2:887369/150‑1 (MQ=255)
                                                                                                               tGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTg                                   <  2:1769439/150‑1 (MQ=255)
                                                                                                                 aTGGATTTGGGATAGTAAGAGTATTCCCCGGGCAGACGACCTGGGAAGGGGAGAGATTTATGCGCGCGTCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                 <  1:1822941/150‑1 (MQ=255)
                                                                                                                 aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAGGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                 <  2:731505/150‑1 (MQ=255)
                                                                                                                 aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                 <  2:1135764/150‑1 (MQ=255)
                                                                                                                  tGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGt                                >  2:1623953/1‑150 (MQ=255)
                                                                                                                    cATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGAAAAATTCGCGCGGCCTTGCTCAAAGCCAAAGACCGTCTGGGAGCGGATAAACTGCGCGCGGAACATTTCACGCTCACGCGCGCCTTCCGCTGTGTTa                              >  2:924409/1‑150 (MQ=255)
                                                                                                                      tttGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATc                            <  1:818653/150‑1 (MQ=255)
                                                                                                                       ttGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCg                           <  1:59400/150‑1 (MQ=255)
                                                                                                                             gagGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGCAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTgac                     >  2:243818/1‑148 (MQ=255)
                                                                                                                              cggAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                    <  1:887525/149‑1 (MQ=255)
                                                                                                                                gAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCGTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                  <  1:194611/150‑1 (MQ=255)
                                                                                                                                  ctccccttCCCCGGTCGTCTGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaa                >  1:1010382/7‑150 (MQ=255)
                                                                                                                                  agagTATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaa                <  2:1272006/150‑1 (MQ=255)
                                                                                                                                   gagTATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaag               <  1:982323/150‑1 (MQ=255)
                                                                                                                                     gTATTCCCCGGTCAGACGACCGGGGATGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaagta             >  1:492717/1‑148 (MQ=255)
                                                                                                                                          ccccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGa        <  1:618953/150‑1 (MQ=255)
                                                                                                                                          ccccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGGCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGa        <  1:299820/150‑1 (MQ=255)
                                                                                                                                           cccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGAt       >  1:1869071/1‑150 (MQ=255)
                                                                                                                                               gTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCg   <  1:718667/150‑1 (MQ=255)
                                                                                                                                                tCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGa  >  2:1094208/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGA  >  NC_000913/4283084‑4283377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: