Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,297,195 A→T 5.6% intergenic (‑70/+24) yjcO ← / ← fdhF Sel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,297,1950AT 5.6% 390.3 / 9.4 139intergenic (‑70/+24)yjcO/fdhFSel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base A (71/60);  new base T (5/3);  total (76/63)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 143 total aligned reads displayed.

AAATACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCG  >  NC_000913/4297046‑4297343
                                                                                                                                                     |                                                                                                                                                    
aaaTACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGaa                                                                                                                                                      <  1:1258142/150‑1 (MQ=255)
 aaTACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGaaa                                                                                                                                                     >  2:714700/1‑150 (MQ=255)
       ggCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTATCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGc                                                                                                                                               >  2:895751/1‑150 (MQ=255)
        gCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATTAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCt                                                                                                                                              >  1:449662/1‑150 (MQ=255)
         ttGCCAGGCTCGGAGTCGTTGGCGTTGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGTGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTg                                                                                                                                             <  1:1280343/149‑1 (MQ=255)
          tgacAGGCTCGGAGTCGTTGGCGTGGTCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGt                                                                                                                                            <  1:1586315/147‑1 (MQ=255)
           gCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATATAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTa                                                                                                                                           <  1:45806/150‑1 (MQ=255)
            ccAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTAAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGTGTAGGGAGTAACCAGTATAATGAGTTCGAATGGATAAAAAAACAGACTCCGAAAGGAGGCTGTat                                                                                                                                          >  1:1510354/1‑149 (MQ=255)
             cAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGtttt                                                                                                                                         <  2:747061/150‑4 (MQ=255)
             cAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGtttt                                                                                                                                         <  1:1441122/150‑4 (MQ=255)
                gCTCGGAGTCGTTGGCGGGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATATTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAg                                                                                                                                      <  2:1719210/150‑1 (MQ=255)
                  tCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGa                                                                                                                                    <  1:1891195/150‑1 (MQ=255)
                   cGGAGTCGTTGGAGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGAc                                                                                                                                   >  2:436679/1‑150 (MQ=255)
                    ggAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTTATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACg                                                                                                                                  <  1:851070/150‑1 (MQ=255)
                     gAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACgg                                                                                                                                 <  2:1085571/150‑1 (MQ=255)
                     gAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACgg                                                                                                                                 <  2:1545690/150‑1 (MQ=255)
                     gAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACgg                                                                                                                                 <  1:1058012/150‑1 (MQ=255)
                       gTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTa                                                                                                                               <  2:1050225/150‑1 (MQ=255)
                        tCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATTATTTTTTTCATTTTGATAATACGATAGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACACCCTCCGAAAGGAGGCTGTAGAAAGTACGGTAt                                                                                                                              >  1:256278/1‑150 (MQ=255)
                        tCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAt                                                                                                                              <  1:988775/150‑1 (MQ=255)
                         cGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAtt                                                                                                                             >  2:1904498/1‑150 (MQ=255)
                          gTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTa                                                                                                                            >  2:129095/1‑150 (MQ=255)
                          gTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTa                                                                                                                            <  1:694075/150‑1 (MQ=255)
                             ggCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGc                                                                                                                         >  1:1081838/1‑150 (MQ=255)
                             ggCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGc                                                                                                                         >  1:1339238/1‑150 (MQ=255)
                                gTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAg                                                                                                                      >  2:209995/1‑150 (MQ=255)
                                  gggCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTg                                                                                                                    <  2:1519627/150‑1 (MQ=255)
                                   ggCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGc                                                                                                                   >  1:186386/1‑150 (MQ=255)
                                   ggCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGATGTAGAAAGGACGGGATTACGCCAGTGc                                                                                                                   >  2:345278/1‑150 (MQ=255)
                                    gCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGcc                                                                                                                  >  2:1807051/1‑150 (MQ=255)
                                     cAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTTATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCg                                                                                                                 >  2:482735/1‑150 (MQ=255)
                                     cAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCg                                                                                                                 >  1:1721518/1‑150 (MQ=255)
                                     cAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAAAACGATTGGGTAGGGAGTAACCACTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAATACGCCAGTGccc                                                                                                                 >  2:1752407/1‑149 (MQ=255)
                                      aaaGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGc                                                                                                                >  1:154625/1‑150 (MQ=255)
                                      aaaGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGACAGTGCCGc                                                                                                                >  1:736259/1‑150 (MQ=255)
                                         gAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACTGTATTACGCCAGTGCCGCtta                                                                                                             >  1:505412/1‑149 (MQ=255)
                                          aaTGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTACCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTcg                                                                                                            >  1:939479/1‑150 (MQ=255)
                                          aaTGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTcg                                                                                                            <  1:184551/150‑1 (MQ=255)
                                           aTGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTTGAAAGGACGGTATTACGCCAGTGCCGCTTcgc                                                                                                           >  2:133108/1‑150 (MQ=255)
                                             gTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCgcgc                                                                                                         >  2:512590/1‑150 (MQ=255)
                                              tCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCa                                                                                                        >  2:1537836/1‑150 (MQ=255)
                                              tCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCAGAATGGAGGCTGTAGAAAGTACGGTATTACGCCAGTGCAGCTTCGCGCa                                                                                                        >  2:65171/1‑150 (MQ=255)
                                               cAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAg                                                                                                       >  2:387975/1‑150 (MQ=255)
                                               cAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAAAACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAAAAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAg                                                                                                       >  2:833157/1‑150 (MQ=255)
                                                aaaaaCAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAgg                                                                                                      <  1:1599888/150‑1 (MQ=255)
                                                     caacaTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCAGCTTAGCGCAGGCGAGt                                                                                                 >  2:92857/1‑150 (MQ=255)
                                                       acaTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGttt                                                                                               <  2:556914/150‑1 (MQ=255)
                                                        caTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGtttt                                                                                              >  1:21246/1‑150 (MQ=255)
                                                        caTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGtttt                                                                                              >  2:874576/1‑150 (MQ=255)
                                                          tccaTGCGATAATTTTTATCATTATGATAATACGATTGGGTCGGGAGTAACCAGTCTACAGAGTTCGAATCGATAAAAAAACAGCCTCCGAACGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCa                                                                                            <  2:287917/147‑1 (MQ=255)
                                                          tCAATTCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCa                                                                                            <  1:1115433/150‑1 (MQ=255)
                                                          tCAATGCGATAATTTTATTCATTTTGCTAATACGAATGCGTCGGGAGTAACCAGTCTAAAGCGGTCGAATGGATAAAAAACCAGCCGCCGAACGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCa                                                                                            <  1:169624/150‑1 (MQ=255)
                                                           cAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCaa                                                                                           >  2:1567824/1‑150 (MQ=255)
                                                             aTGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACt                                                                                         <  1:1223342/150‑1 (MQ=255)
                                                               gCGATAATTTTTTTCATTTTGATAATACGATTGGGGAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttg                                                                                       <  2:1692416/150‑1 (MQ=255)
                                                                 taTAATTTTTTTCATTTTGATAATACGATTTGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgtt                                                                                     <  1:375522/149‑1 (MQ=255)
                                                                  aTAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgttg                                                                                    <  1:345547/150‑1 (MQ=255)
                                                                  aTAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgttg                                                                                    <  1:1248481/150‑1 (MQ=255)
                                                                  aTAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGAAGGCGAGTTTTCAACttgttt                                                                                    >  1:1435830/1‑149 (MQ=255)
                                                                   tAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAATGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTtgttgt                                                                                   >  1:320799/1‑150 (MQ=255)
                                                                     aTTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTAc                                                                                 >  2:1815284/1‑150 (MQ=255)
                                                                      tttttttCATTTTGATTATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTTCCGCTTCGCGCACGCGAGTTTTCAACTTGTTGTAca                                                                                >  1:1185200/1‑149 (MQ=255)
                                                                          tttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGATTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCACTGCCGCTAAGCGCAGGCGAGTTTTCCACATGTTGTACTCGTc                                                                            >  2:1180669/1‑150 (MQ=255)
                                                                          tttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTc                                                                            >  1:1625155/1‑150 (MQ=255)
                                                                             cATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAAt                                                                         >  2:818458/1‑150 (MQ=255)
                                                                               ttttGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCa                                                                       >  1:767850/1‑150 (MQ=255)
                                                                                 ttGATAATACGATTGGGTAGGGAGTAACCATTATAAAGAGTTCGAATGGATAAATAAACAGCCTCCGTAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACg                                                                     <  1:705684/150‑1 (MQ=255)
                                                                                        taCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCt                                                              <  1:619168/150‑1 (MQ=255)
                                                                                          cGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTcg                                                            >  1:849033/1‑150 (MQ=255)
                                                                                          cGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATAACGTACTGCTcg                                                            >  1:1163563/1‑150 (MQ=255)
                                                                                               gggTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCggcggc                                                       >  2:1443041/1‑150 (MQ=255)
                                                                                               gggTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCggcggc                                                       >  1:1670085/1‑150 (MQ=255)
                                                                                                 ttAGGGAGTAACCAGTATAAAGAGTTCTAATGGATAAAAAAACAGACTCAGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGgcgc                                                     <  2:617092/149‑1 (MQ=255)
                                                                                                 gTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGGCAATCACGTACTGCTCGGCGgcgc                                                     <  2:302438/150‑1 (MQ=255)
                                                                                                    gggAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCt                                                  <  2:1466887/150‑1 (MQ=255)
                                                                                                          aaCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcgg                                            >  2:1117009/1‑150 (MQ=255)
                                                                                                          aaCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcgg                                            >  2:757740/1‑150 (MQ=255)
                                                                                                          aaCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcgg                                            <  1:536132/150‑1 (MQ=255)
                                                                                                           tccAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggc                                           <  1:895751/149‑1 (MQ=255)
                                                                                                               gTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatc                                       <  2:1838132/150‑1 (MQ=255)
                                                                                                                tataAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcg                                      <  1:784501/150‑1 (MQ=255)
                                                                                                                  taAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcggc                                    <  1:209995/150‑1 (MQ=255)
                                                                                                                  taAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTAGTAGTACTCGGCAAGCACGTACTGCTCGGCGACGCGCAcatcggcgctcggc                                    >  1:929573/1‑150 (MQ=255)
                                                                                                                   aaaGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGGCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCt                                   <  2:1127241/150‑1 (MQ=255)
                                                                                                                    aaGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTc                                  <  1:1154865/150‑1 (MQ=255)
                                                                                                                       agTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCgcc                               >  2:1623994/1‑148 (MQ=255)
                                                                                                                               tGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACgg                       <  1:1360270/150‑1 (MQ=255)
                                                                                                                                 gATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGATAGGCCGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcg                     >  2:604502/1‑150 (MQ=255)
                                                                                                                                  aTAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcgc                    >  2:1464296/1‑150 (MQ=255)
                                                                                                                                  aTAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcgc                    >  1:1519559/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAg                  >  2:1017567/1‑150 (MQ=255)
                                                                                                                                     tttctaCAGCCTCCTTTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGt                 >  1:1675576/6‑150 (MQ=255)
                                                                                                                                     aaaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGt                 >  1:1755008/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCACGCGAGTTTTCAACTTGATGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGt                 >  1:691695/1‑150 (MQ=255)
                                                                                                                                         taCAGCCTCCTTTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAttt             >  2:959614/2‑150 (MQ=255)
                                                                                                                                          ccAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTg            <  1:312175/149‑1 (MQ=255)
                                                                                                                                           cAGCCTCCGGTCGGAGGCTGTAGAAAGGCCGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCCACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGCTCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTgg           >  1:70439/1‑149 (MQ=255)
                                                                                                                                               cTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCTGTATTTGTACTc       >  2:1889172/1‑150 (MQ=255)
                                                                                                                                                  cGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCTGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCgg    <  1:752911/150‑1 (MQ=255)
                                                                                                                                                    aaaGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCg  <  1:1537836/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAATACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCG  >  NC_000913/4297046‑4297343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑