Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,134 C→A 5.8% A75S (GCA→TCA) ‡ cutA ← divalent‑cation tolerance protein, copper sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,1340CA 5.8% 409.2 / 11.7 137A75S (GCA→TCA) ‡cutAdivalent‑cation tolerance protein, copper sensitivity
Reads supporting (aligned to +/- strand):  ref base C (76/52);  new base A (4/4);  total (80/57)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

Only 100 of 137 total aligned reads displayed.

GCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATCC  >  NC_000913/4364987‑4365282
                                                                                                                                                   |                                                                                                                                                    
gCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCct                                                                                                                                                    >  1:1736919/1‑150 (MQ=255)
gCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCct                                                                                                                                                    <  2:770442/150‑1 (MQ=255)
 cAAAAACGGAAGTGCTGCAAAGTAGCAGGATCATCGTAAAGATGCGTTGAGCCATGAGACGTAATCTGTGTCTCCGTGTGGCACAGGTAAACCCAGAAGTTCCGGGGTGTGATATGGATGATGCGACTTCATGCATTCCAGCAGTGCctg                                                                                                                                                   >  1:935877/1‑150 (MQ=255)
       cGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTg                                                                                                                                             >  1:576850/1‑150 (MQ=255)
         taaGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaatg                                                                                                                                           <  2:1876236/149‑5 (MQ=255)
         gAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCTTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGGGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTgag                                                                                                                                           <  1:1581087/150‑1 (MQ=255)
         gAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaatg                                                                                                                                           <  1:3560/150‑5 (MQ=255)
             tgctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATAc                                                                                                                                       <  1:555060/150‑1 (MQ=255)
              gctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACg                                                                                                                                      <  1:1570834/150‑1 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTACAGATGCGTTGAGCCATGAGAGGTCATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    <  2:1817952/150‑1 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    >  1:1040484/1‑150 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    >  2:1728313/1‑150 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    >  2:409472/1‑150 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    >  2:836856/1‑150 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    >  2:844127/1‑150 (MQ=255)
                tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACAAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                                    >  2:1022408/1‑150 (MQ=255)
                 gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                   >  1:775128/1‑150 (MQ=255)
                 gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                   >  1:1298110/1‑150 (MQ=255)
                 gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTGGATATGTATGGTGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                   >  1:1315064/1‑150 (MQ=255)
                  cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAg                                                                                                                                  >  2:1563585/1‑150 (MQ=255)
                    aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGtt                                                                                                                                >  2:862263/1‑150 (MQ=255)
                    aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGAGGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGtt                                                                                                                                <  1:1498950/150‑1 (MQ=255)
                      gTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGtttt                                                                                                                              >  1:1503120/1‑150 (MQ=255)
                      gTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGtttt                                                                                                                              >  1:686703/1‑150 (MQ=255)
                       tAGCAGGATCAGCGTAAAGATTCGTTGAGCCATGAGATGTAATCTGTGTATCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATTAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGttttt                                                                                                                             <  1:1185591/150‑1 (MQ=255)
                        aGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTa                                                                                                                            >  2:193907/1‑150 (MQ=255)
                          cAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTaaa                                                                                                                          <  1:1405930/150‑1 (MQ=255)
                            ggATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAat                                                                                                                        <  2:797750/150‑1 (MQ=255)
                                  gagTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTg                                                                                                                  <  2:1148556/148‑1 (MQ=255)
                                   cttAAAGATGCGTTGAGGCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTTATACCAGATGTTACGGGGTTTGATATGTATGATGAGACTTCAGGCATTCCAGCACTGGCTGCTGGTGAGATACGGTACTTTTTAAAATCATCTGc                                                                                                                 <  1:1865799/148‑1 (MQ=255)
                                   cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                                 <  2:1791880/150‑1 (MQ=255)
                                      aaaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACt                                                                                                              <  1:208010/150‑1 (MQ=255)
                                      aaaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACt                                                                                                              <  2:502019/150‑1 (MQ=255)
                                        aGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTc                                                                                                            <  2:313660/150‑1 (MQ=255)
                                           tGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTa                                                                                                         >  2:911111/1‑150 (MQ=255)
                                             cGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTAtt                                                                                                       >  1:721532/1‑150 (MQ=255)
                                              gTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTc                                                                                                      <  1:618601/150‑1 (MQ=255)
                                              gTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTc                                                                                                      <  2:1022053/150‑1 (MQ=255)
                                                  aGCCATGAGAGGTAATCTGTCTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGc                                                                                                  <  1:1786071/150‑1 (MQ=255)
                                                    ccATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTc                                                                                                >  2:49925/1‑150 (MQ=255)
                                                     cATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTcc                                                                                               <  2:127724/150‑1 (MQ=255)
                                                      gtGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCa                                                                                              >  1:408916/2‑150 (MQ=255)
                                                      aTGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCa                                                                                              >  1:1879177/1‑150 (MQ=255)
                                                       tGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAg                                                                                             <  1:1261051/150‑1 (MQ=255)
                                                        gagaGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGc                                                                                            <  2:223402/150‑1 (MQ=255)
                                                         agagGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCt                                                                                           <  1:1645628/150‑1 (MQ=255)
                                                            ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                        >  1:1644014/1‑150 (MQ=255)
                                                            ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                        <  1:1708517/150‑1 (MQ=255)
                                                            ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                        >  1:778388/1‑150 (MQ=255)
                                                             gTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAcc                                                                                       >  2:405389/1‑150 (MQ=255)
                                                              tAATCTGTGTCTCCATGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCt                                                                                      >  2:1340192/1‑150 (MQ=255)
                                                                aTCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTc                                                                                    >  2:1090463/1‑150 (MQ=255)
                                                                   tgtgTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCa                                                                                 <  1:501816/150‑1 (MQ=255)
                                                                   tgtgTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCATGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACATTACCa                                                                                 >  2:1219458/1‑150 (MQ=255)
                                                                    gtgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAg                                                                                <  1:1777740/150‑1 (MQ=255)
                                                                     tgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGt                                                                               <  2:1618417/150‑1 (MQ=255)
                                                                        ctcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAAt                                                                            >  1:1128316/1‑150 (MQ=255)
                                                                          ccGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATag                                                                          >  1:937632/1‑150 (MQ=255)
                                                                            gtgtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagag                                                                        <  1:81369/150‑1 (MQ=255)
                                                                            gtgtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagag                                                                        <  1:795502/150‑1 (MQ=255)
                                                                              gtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagag                                                                      >  1:1808056/1‑150 (MQ=255)
                                                                               tgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagaga                                                                     <  1:961704/150‑1 (MQ=255)
                                                                                gtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagagag                                                                    >  2:500765/1‑150 (MQ=255)
                                                                                  aaCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCAAGTAATATATAGAGGt                                                                  >  2:606646/1‑150 (MQ=255)
                                                                                   aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTa                                                                 >  1:1562878/1‑150 (MQ=255)
                                                                                   aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTa                                                                 >  1:270211/1‑150 (MQ=255)
                                                                                   aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTa                                                                 >  1:14071/1‑150 (MQ=255)
                                                                                           aaCCAGGAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCggg                                                         >  2:819945/1‑150 (MQ=255)
                                                                                              cagcagTTCCGTGGTTTGATATGGATTATGAGACTTCAGGCATTCCAGCAGTGCCTGATGGTGAGATACGGTAGTTTTTAACATCATCTGCCCTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGTGGAt                                                      <  2:900309/150‑1 (MQ=255)
                                                                                              cAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGAt                                                      >  1:496049/1‑150 (MQ=255)
                                                                                                gAAGTTCCTGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGAAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                    <  2:356357/150‑1 (MQ=255)
                                                                                                gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                    <  1:408858/150‑1 (MQ=255)
                                                                                                    ttCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGt                                                <  2:1006472/150‑1 (MQ=255)
                                                                                                        ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTcgcg                                            >  2:229934/1‑150 (MQ=255)
                                                                                                           gTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAg                                         >  1:1840337/1‑150 (MQ=255)
                                                                                                            gttGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAgg                                        <  2:1188718/149‑1 (MQ=255)
                                                                                                             ttGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgc                                       <  1:979125/150‑1 (MQ=255)
                                                                                                              tGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgcc                                      >  1:478530/1‑150 (MQ=255)
                                                                                                                 aatGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgccgcc                                   <  1:388758/149‑1 (MQ=255)
                                                                                                                          tgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCGCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTcc                          >  2:373833/1‑150 (MQ=255)
                                                                                                                          tgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTcc                          >  2:54817/1‑150 (MQ=255)
                                                                                                                          tgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTcc                          >  2:193554/1‑150 (MQ=255)
                                                                                                                          tgaGACTTAAGGCATTCCAGCAGGGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTcc                          <  2:246914/150‑1 (MQ=255)
                                                                                                                           gagaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCg                         >  2:1335502/1‑150 (MQ=255)
                                                                                                                           gagaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTATAAAATCATCAGCACAGCGTATTCTTGCTCCAGCTTCCCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCg                         <  1:325964/150‑1 (MQ=255)
                                                                                                                             gaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATTGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGcc                       >  1:1693998/1‑150 (MQ=255)
                                                                                                                             gaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGcc                       >  1:31947/1‑150 (MQ=255)
                                                                                                                               cTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAg                     >  1:1637825/1‑150 (MQ=255)
                                                                                                                                  cAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCAc                  >  1:22311/1‑150 (MQ=255)
                                                                                                                                      cATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg              >  2:1040163/1‑150 (MQ=255)
                                                                                                                                      cATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg              >  2:1647182/1‑150 (MQ=255)
                                                                                                                                       tcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAgg                                        >  1:1905621/4‑123 (MQ=255)
                                                                                                                                        caccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGATCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggc            >  2:1733552/3‑150 (MQ=255)
                                                                                                                                         tCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACATCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcg           >  1:376872/1‑150 (MQ=255)
                                                                                                                                         accAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcg           >  2:1218391/2‑150 (MQ=255)
                                                                                                                                         accAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcg           >  2:347164/2‑150 (MQ=255)
                                                                                                                                           cagcagTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcggc         >  1:1543850/1‑150 (MQ=255)
                                                                                                                                             gcagTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTa       >  1:1839759/1‑150 (MQ=255)
                                                                                                                                                  gCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATcc  >  2:1392070/1‑150 (MQ=255)
                                                                                                                                                  gCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATcc  <  2:1298110/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATCC  >  NC_000913/4364987‑4365282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: