BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	440,663	T→G	5.4%	S147R (AGC→CGC) ‡	ispA ←	geranyltranstransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	440,663	0	T	G	5.4%	305.7 / 5.0	106	S147R (AGC→CGC) ‡	ispA	geranyltranstransferase
Reads supporting (aligned to +/- strand): ref base T (47/53); new base G (2/4); total (49/57)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 107 total aligned reads displayed.

GCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGCAAA  >  NC_000913/440514‑440811

gcTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCt                                                                                                                                                      >  1:685318/1‑150 (MQ=255)

   tAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGc                                                                                                                                                   <  1:596483/150‑1 (MQ=255)

    actGCACCAAGGCGACCCGCGGCGAGAATCAATGCGCCGGGTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGATTCCCTTCCGCGTCTACATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGcc                                                                                                                                                  <  1:358531/148‑1 (MQ=255)

      tGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAg                                                                                                                                                >  1:1261833/1‑150 (MQ=255)

      tGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAg                                                                                                                                                <  2:595515/150‑1 (MQ=255)

       gCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGt                                                                                                                                               >  1:799324/1‑150 (MQ=255)

        cACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGAGCGCCAGtg                                                                                                                                              <  2:1321411/150‑2 (MQ=255)

         aCCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTc                                                                                                                                             <  1:4084/150‑1 (MQ=255)

           cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGAGCGCCAGtggta                                                                                                                                           <  1:437963/150‑5 (MQ=255)

           cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGAGCGCCAGtggta                                                                                                                                           <  1:936471/150‑5 (MQ=255)

            aaGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGa                                                                                                                                          <  2:537050/150‑1 (MQ=255)

               gCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCCCCGCCCATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGCAat                                                                                                                                       >  1:1399643/1‑150 (MQ=255)

                   aCGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcatc                                                                                                                                   <  1:1726560/150‑1 (MQ=255)

                    cggcggCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCg                                                                                                                                  <  2:331306/150‑1 (MQ=255)

                    cggcggCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCg                                                                                                                                  <  2:1115535/150‑1 (MQ=255)

                     ggcggcGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGATATCATCGa                                                                                                                                 >  1:1009266/1‑150 (MQ=255)

                          cgcgAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTc                                                                                                                            <  1:1466967/150‑1 (MQ=255)

                          cgcgAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGAGTCCAGAGGTACGTGATTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTc                                                                                                                            >  1:1801037/1‑150 (MQ=255)

                              aaTCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTc                                                                                                                        <  1:404756/150‑1 (MQ=255)

                               aTCAATGCGCCGGTATTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTcg                                                                                                                       >  1:1442306/1‑150 (MQ=255)

                                tCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTcgc                                                                                                                      <  2:1548774/150‑1 (MQ=255)

                                   aTGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGt                                                                                                                   <  1:299121/150‑1 (MQ=255)

                                       gcCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGa                                                                                                               <  1:747425/150‑1 (MQ=255)

                                               tATGACGATGAATACGCTCAAGCGCGGCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCg                                                                                                       <  1:42989/150‑1 (MQ=255)

                                                aTGACGATCAATACGCTCAAGCGCGTCCAGAGGAACGTGTTCGCCTGCCGCGTCTAAATCTACTGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCgg                                                                                                      <  2:1392369/150‑1 (MQ=255)

                                                        gAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCgg                                                                                              >  1:958980/1‑150 (MQ=255)

                                                         aaTACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGc                                                                                             <  2:844296/150‑1 (MQ=255)

                                                         aaTACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGc                                                                                             <  2:837007/150‑1 (MQ=255)

                                                            aCGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACAACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGGCCGACACTTCCGGCATATCGGCATc                                                                                          <  2:410923/150‑1 (MQ=255)

                                                               cTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCTATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCGGGCATATCGGCATCGCt                                                                                       >  2:1473380/1‑150 (MQ=255)

                                                               cTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCt                                                                                       <  2:1616587/150‑1 (MQ=255)

                                                               cTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCt                                                                                       >  2:1480626/1‑150 (MQ=255)

                                                               cTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCt                                                                                       >  2:1473379/1‑150 (MQ=255)

                                                                tCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCtt                                                                                      <  2:1286969/150‑1 (MQ=255)

                                                                  aaGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTaa                                                                                    <  1:1743608/150‑1 (MQ=255)

                                                                    gcgcGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTaaaa                                                                                  >  2:1307642/1‑150 (MQ=255)

                                                                     cgcgTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCTAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAAt                                                                                 <  1:1355232/150‑1 (MQ=255)

                                                                       cgTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGGAATACCACTGGCGCTCGCCAGTTCTGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCAGCGCTTAAAATCg                                                                               <  1:1229477/150‑1 (MQ=255)

                                                                        gTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCga                                                                              <  2:667586/150‑1 (MQ=255)

                                                                         tCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGCCCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAAGCgag                                                                             >  2:832022/1‑150 (MQ=255)

                                                                          ccAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCgaga                                                                            <  1:843627/150‑1 (MQ=255)

                                                                               ggTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCGAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGcc                                                                       <  1:156342/150‑1 (MQ=255)

                                                                               ggTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGcc                                                                       >  2:760946/1‑150 (MQ=255)

                                                                                 tACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAg                                                                     <  2:1678488/150‑1 (MQ=255)

                                                                                   cGTGTTTGCCTTCCGCGTCTAACTCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCg                                                                   <  2:1461999/150‑1 (MQ=255)

                                                                                   cGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCg                                                                   >  1:1660523/1‑150 (MQ=255)

                                                                                   cGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCAGTCTATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGCCACGTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCg                                                                   >  1:305865/1‑150 (MQ=255)

                                                                                      gtTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGttt                                                                <  2:1376515/150‑1 (MQ=255)

                                                                                       tttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTg                                                               <  2:210434/150‑1 (MQ=255)

                                                                                       tttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTg                                                               <  1:1691212/150‑1 (MQ=255)

                                                                                       tttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTg                                                               <  1:1797978/150‑1 (MQ=255)

                                                                                       tttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCACATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTg                                                               >  1:1711706/1‑150 (MQ=255)

                                                                                        ttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGt                                                              <  1:1397370/150‑1 (MQ=255)

                                                                                         tGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAATTCGAGAACGCCAGCGTTTGTa                                                             >  1:1677800/1‑150 (MQ=255)

                                                                                           ccTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACTCCCGCGTTTGTaaa                                                           >  1:1695155/1‑150 (MQ=255)

                                                                                            cTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAg                                                          >  2:1553723/1‑150 (MQ=255)

                                                                                                cgcgTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTc                                                      >  2:1858248/1‑150 (MQ=255)

                                                                                                cgcgTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTc                                                      >  1:670381/1‑150 (MQ=255)

                                                                                                  cgTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGc                                                    <  1:249657/150‑1 (MQ=255)

                                                                                                  cgTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCGCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGc                                                    <  2:809645/150‑1 (MQ=255)

                                                                                                     cTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGGCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAg                                                 <  2:685318/150‑1 (MQ=255)

                                                                                                      tAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGc                                                >  1:1250167/1‑150 (MQ=255)

                                                                                                       aaaTCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCg                                               >  1:444531/1‑150 (MQ=255)

                                                                                                         aTCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCgag                                             >  2:878010/1‑150 (MQ=255)

                                                                                                         aTCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCgag                                             <  2:452971/150‑1 (MQ=255)

                                                                                                          tCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCGTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCgaga                                            >  1:1500945/1‑150 (MQ=255)

                                                                                                           cTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGaa                                           >  2:473658/1‑150 (MQ=255)

                                                                                                               tGCCTGACCCCCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCATAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTCAAGCGTCGCCAGCGAGAATcgc                                       >  2:1540273/1‑150 (MQ=255)

                                                                                                               tGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATcgc                                       >  1:1614361/1‑150 (MQ=255)

                                                                                                               tGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATcgc                                       >  1:1351344/1‑150 (MQ=255)

                                                                                                               tGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATcgc                                       >  2:1049628/1‑150 (MQ=255)

                                                                                                                    gACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTg                                  >  2:1514591/1‑150 (MQ=255)

                                                                                                                    gACCACCGCACATTCCGGCAATACCACTGGCGATCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGATCCGCCACTTCCGGCATATCAGCATAGCTTAAAATCGAGAACGCCAGCGTTTCTAAAGCGTCGCCAGCGAGAATCGCGTATg                                  >  1:325316/1‑150 (MQ=255)

                                                                                                                     accaccGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGc                                 <  2:720094/150‑1 (MQ=255)

                                                                                                                        accGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTc                              >  2:1733855/1‑150 (MQ=255)

                                                                                                                        accGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTc                              <  2:1769992/150‑1 (MQ=255)

                                                                                                                         ccGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCg                             <  1:460529/150‑1 (MQ=255)

                                                                                                                         ccGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCg                             <  1:1858248/150‑1 (MQ=255)

                                                                                                                         ccGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGAATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCAACAGAGAGAATCGCGTTTGCTTCg                             >  1:929306/1‑150 (MQ=255)

                                                                                                                           gCACATTCCGGCAATACCTCTGGCGCTCGCCAGTTCAGACATCATCGACATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCGTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGcc                           <  2:307002/150‑1 (MQ=255)

                                                                                                                           gCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGcc                           >  1:558365/1‑150 (MQ=255)

                                                                                                                           gCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGcc                           <  2:817125/150‑1 (MQ=255)

                                                                                                                            cacaTTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGccc                          >  2:1851297/1‑149 (MQ=255)

                                                                                                                              caTTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGCCACTGCCGCCATATCGGCATCGCTTAAAAGAGAGACCGCCAGCGGTTGTAAAGCGTCGCCCGCGAGAATCGCGTTTGCTTCGACata                        >  1:239243/1‑148 (MQ=255)

                                                                                                                                ttCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGTCCTATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCTCCCTCGAGAATCGCGTTAGCTTCGCCAAACt                      >  1:278122/1‑150 (MQ=255)

                                                                                                                                ttCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACt                      >  2:1572364/1‑150 (MQ=255)

                                                                                                                                ttCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACt                      <  2:597526/150‑1 (MQ=255)

                                                                                                                                  ccGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCCGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCACACtgc                    >  1:1724588/1‑148 (MQ=255)

                                                                                                                                  ccGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTc                    <  2:870188/150‑1 (MQ=255)

                                                                                                                                  ccGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTc                    <  2:877809/150‑1 (MQ=255)

                                                                                                                                   cGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTca                   <  1:182190/150‑1 (MQ=255)

                                                                                                                                    ggCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTcac                  >  2:1900419/1‑150 (MQ=255)

                                                                                                                                         tACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGc             <  2:1085292/150‑1 (MQ=255)

                                                                                                                                          tgaaCTGGCGAGCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCa            >  2:1880018/4‑150 (MQ=255)

                                                                                                                                           gaaCTGGCGAGCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAg           >  2:1315554/3‑150 (MQ=255)

                                                                                                                                           ccACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAg           >  2:153954/1‑150 (MQ=255)

                                                                                                                                                 gcgcTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTGGCCTAACTTCACATGGCAGGTTGGc     <  2:459326/150‑1 (MQ=255)

                                                                                                                                                 gcgcTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGc     <  1:1536091/150‑1 (MQ=255)

                                                                                                                                                   gcTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGCaa   <  1:1309103/150‑1 (MQ=255)

                                                                                                                                                    cTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCTCCAAACTTCACATGGCAGGTTGGCaaa  >  1:1882453/1‑150 (MQ=255)

GCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGCAAA  >  NC_000913/440514‑440811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`