Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,473,9270CA100.0% 399.5 / NA 116A43S (GCC→TCC) bdcAc‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (47/69);  total (47/69)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 116 total aligned reads displayed.

GGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473780‑4474073
                                                                                                                                                   |                                                                                                                                                  
gggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAAt                                                                                                                                                  <  1:1749758/150‑1 (MQ=255)
gggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAAt                                                                                                                                                  <  2:727754/150‑1 (MQ=255)
  gCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCt                                                                                                                                                >  2:774754/1‑150 (MQ=255)
    cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCttt                                                                                                                                              <  1:1585892/150‑1 (MQ=255)
    cTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCttt                                                                                                                                              <  1:805425/150‑1 (MQ=255)
     tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  2:900644/150‑1 (MQ=255)
     tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGACTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTc                                                                                                                                             <  1:685108/150‑1 (MQ=255)
      cGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCg                                                                                                                                            <  2:915349/150‑1 (MQ=255)
       gCCAAAGACGCTAATTCCTGCATTTACCACCAGGATATCCAATGCGCCGATCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCTGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGa                                                                                                                                           <  2:1556054/150‑1 (MQ=255)
       gCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGa                                                                                                                                           <  1:1455896/150‑1 (MQ=255)
        ccAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAc                                                                                                                                          <  1:1488066/150‑1 (MQ=255)
             gACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGc                                                                                                                                     <  2:1357518/150‑1 (MQ=255)
             gACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTGTAGCGGAATCTTTCGACCCCGc                                                                                                                                     <  2:789315/150‑1 (MQ=255)
               cGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCAt                                                                                                                                   >  1:1882211/1‑150 (MQ=255)
                gCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATa                                                                                                                                  <  1:868495/150‑1 (MQ=255)
                 ccAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAg                                                                                                                                 >  2:1021300/1‑150 (MQ=255)
                    aTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACATCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTg                                                                                                                              <  1:603378/150‑1 (MQ=255)
                    aTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTg                                                                                                                              <  1:1710920/150‑1 (MQ=255)
                    aTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTg                                                                                                                              <  1:1008351/150‑1 (MQ=255)
                      aCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGaa                                                                                                                            <  1:1217557/150‑1 (MQ=255)
                       ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           >  2:1292732/1‑150 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         <  2:1370283/150‑1 (MQ=255)
                         tGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCg                                                                                                                         >  2:1294288/1‑150 (MQ=255)
                            aTTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTAc                                                                                                                      <  1:1013733/150‑1 (MQ=255)
                                 ccaccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTATAGCGGAATCTTTCGCCCCCGCATAGGTGAATCGTACATTgg                                                                                                                 >  2:107110/1‑150 (MQ=255)
                                   accaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGCATACTGCTGTCGCTCCAGTCGCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAACCCGCATAGGTGAATCGTACATTGGcc                                                                                                               >  2:1410671/1‑150 (MQ=255)
                                   accaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcc                                                                                                               >  1:1655567/1‑150 (MQ=255)
                                    ccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGccc                                                                                                              >  1:1024317/1‑150 (MQ=255)
                                    ccaGCATCTCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTAGCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTGTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGccc                                                                                                              <  1:149003/150‑1 (MQ=255)
                                     caGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGTCAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcccc                                                                                                             <  2:251066/150‑1 (MQ=255)
                                        gATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                                                                                                          <  1:700460/150‑1 (MQ=255)
                                        gATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                                                                                                          <  2:284103/150‑1 (MQ=255)
                                          tatCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCgg                                                                                                        >  2:1264606/1‑150 (MQ=255)
                                            tCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                      >  2:1653647/1‑150 (MQ=255)
                                            tCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                      <  1:1815931/150‑1 (MQ=255)
                                            tCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTCTCGCTCCAGTCTCTTCTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                                                                                                      <  1:285306/150‑1 (MQ=255)
                                             ccAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGa                                                                                                     >  2:225225/1‑150 (MQ=255)
                                              cAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGAc                                                                                                    >  2:556810/1‑150 (MQ=255)
                                                    gccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg                                                                                              <  2:1871724/150‑1 (MQ=255)
                                                       gcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgacg                                                                                           <  1:1002473/150‑1 (MQ=255)
                                                          ctTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAc                                                                                        <  1:30183/150‑1 (MQ=255)
                                                           ttACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACg                                                                                       <  1:557644/150‑1 (MQ=255)
                                                           ttACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACg                                                                                       >  1:879387/1‑150 (MQ=255)
                                                             acgaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGAt                                                                                     >  2:1823277/1‑150 (MQ=255)
                                                              cgaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGCCGTACGATa                                                                                    >  1:181016/1‑150 (MQ=255)
                                                              cgaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATa                                                                                    >  2:485898/1‑150 (MQ=255)
                                                                 acgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCg                                                                                 >  1:404506/1‑150 (MQ=255)
                                                                    ccctCAATGCCAGCGGCTCTGTCAGCACTCTCTGGGAATACGGCTGGCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGAACCCGCATAGGTGTATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCa                                                                              <  2:310178/147‑1 (MQ=255)
                                                                    aCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCGCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCa                                                                              <  1:107860/150‑1 (MQ=255)
                                                                      aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            <  1:1484179/150‑1 (MQ=255)
                                                                        cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCCCCGa                                                                          >  1:1540366/1‑150 (MQ=255)
                                                                        cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  1:223825/1‑150 (MQ=255)
                                                                         aaTGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt                                                                         >  2:19661/1‑150 (MQ=255)
                                                                                gCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGa                                                                  >  1:800576/1‑150 (MQ=255)
                                                                                 cGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGAGTTTAGCGGAATCTTTCGACACCGCATAGGTGAATCGTACATTGGCACCATCGGTGACGAAACGACGTAAGATAGCGGCACCGATACCACGaa                                                                 >  1:1334545/1‑149 (MQ=255)
                                                                                   tctcTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTg                                                               <  2:545464/150‑1 (MQ=255)
                                                                                     tctGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGcc                                                             >  2:25244/1‑150 (MQ=255)
                                                                                      ctGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGGCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCCTCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCa                                                            <  1:164418/150‑1 (MQ=255)
                                                                                        gTCAGCACTATCTGTGAATACTGCAGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAcc                                                          >  1:873002/1‑150 (MQ=255)
                                                                                        gTCAGCACTATCCGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAcc                                                          >  1:676275/1‑150 (MQ=255)
                                                                                          cAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGa                                                        >  2:1214025/1‑150 (MQ=255)
                                                                                          cAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGa                                                        <  2:1486023/150‑1 (MQ=255)
                                                                                           aGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAg                                                       <  1:709822/150‑1 (MQ=255)
                                                                                            gCACTATCTGTGAATACTGCTGTCTCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAgg                                                      <  1:864955/150‑1 (MQ=255)
                                                                                               cTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATg                                                   >  2:1034540/1‑150 (MQ=255)
                                                                                                 aTCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgag                                                 <  2:498653/150‑1 (MQ=255)
                                                                                                 aTCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgag                                                 <  2:1613459/150‑1 (MQ=255)
                                                                                                 aTCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGGGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgag                                                 <  2:1459155/150‑1 (MQ=255)
                                                                                                  tCTGTGTATACTGCTGTCGCTCCAGTCTGTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGAACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                <  2:1025782/150‑1 (MQ=255)
                                                                                                    tgtgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAAc                                              >  2:1302887/1‑150 (MQ=255)
                                                                                                     gtgAATACTGCTGTCGATCCAGTCTCTTGTGCCAGGAGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGACCCATCGGTGACGAAACGGCGTACGCTAGCGGCACCGATTCCACGACTGGCACCGAGGTTGAGAAcg                                             >  2:932009/1‑149 (MQ=255)
                                                                                                      tgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGCCTGCCACCGAGGATGAGAACTg                                            >  1:1853762/1‑150 (MQ=255)
                                                                                                       gAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGCCCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGCCGAAACGACGTACGATAGCGGCACCGATACCCCGCCTGCCACCGAGGATGAGAACTGt                                           >  2:1365198/1‑150 (MQ=255)
                                                                                                       gAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGt                                           <  1:1466329/150‑1 (MQ=255)
                                                                                                        aaTACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTc                                          <  1:1264606/150‑1 (MQ=255)
                                                                                                           aCTGCTGTCGCGCCAGGCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTa                                       <  1:938664/150‑1 (MQ=255)
                                                                                                            ctgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAc                                      >  1:1717825/1‑150 (MQ=255)
                                                                                                            ctgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACAACGACTGCCACCGAGGATGAGAACTGTCTTAc                                      >  1:1637773/1‑150 (MQ=255)
                                                                                                             tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     <  1:1418889/150‑1 (MQ=255)
                                                                                                              gctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCt                                    <  2:64895/150‑1 (MQ=255)
                                                                                                               ctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTg                                   <  2:1890028/150‑1 (MQ=255)
                                                                                                                 gTCGCTCCCGTCACTTGGGCCAGCCGTTTAGCGGAATCTTTCGACCCCGCATAGGGGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGCTAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTa                                 <  2:1196898/150‑1 (MQ=255)
                                                                                                                 gTCGCTCCAGTCTCTTGTGCCAGGCGTTATGCGGATTCTTTCGCCCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGAGGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGATCTGTCTTACCTGTa                                 <  1:400361/150‑1 (MQ=255)
                                                                                                                  gcGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTaa                                <  2:1063418/149‑1 (MQ=255)
                                                                                                                       ccAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgcg                           <  1:1021300/150‑1 (MQ=255)
                                                                                                                          ttctcTTGTGCCAGGCGTTTAGCGGAATCTTTCGAACCCGCATAGGTTAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGccc                        <  1:1009323/149‑1 (MQ=255)
                                                                                                                             tctTTTGCCAGTCGTTTAGCGGAATCTTTCAAACACGCATAGGTGAATCGTACATTGGCCACATCTGAGACGAAAAGACGTACGATATCGGCACCGATACCAAGACTGCCAACGAGTATGAGAACTCTCTTACCTGTAAAAGCTCCCATa                     >  1:110652/1‑150 (MQ=255)
                                                                                                                             tctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATa                     <  1:1604973/150‑1 (MQ=255)
                                                                                                                              ctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTACAAGCGCCCATaa                    >  1:210791/1‑150 (MQ=255)
                                                                                                                                tgtgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAgg                  <  2:1882211/150‑1 (MQ=255)
                                                                                                                                tgtgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATACCGGCACCGATACCACCACTGCCACCGAGGATGAGAACTGACTTACCTGTAAAAGTGCCAATTAgg                  >  2:64367/1‑150 (MQ=255)
                                                                                                                                  tgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACGGCCACCGAGGATGAGAACTGTCTTCCCTGTAAAAGCGCCCATAAGGAc                >  2:1792190/1‑150 (MQ=255)
                                                                                                                                   gCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACt               >  1:923535/1‑150 (MQ=255)
                                                                                                                                        gCGGTTAGCGGATTCTTTCGACCCCGCATAGGTGAATCGTACAATGGCCCCATCCGTGACGAAACGACGTACGATATCGGCACCGATACCACTACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAATGACTCCTTg          <  2:79906/150‑1 (MQ=255)
                                                                                                                                          gTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAt        <  1:604990/150‑1 (MQ=255)
                                                                                                                                            ttAGCTGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAttt      <  2:1593564/150‑1 (MQ=255)
                                                                                                                                            ttAGCGGAATCTTGCGCCCCCGCATAGGGGAAGCGTACATTGGCCCCATCGGGGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAttt      <  2:1530204/150‑1 (MQ=255)
                                                                                                                                               gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   >  2:621638/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  >  2:1563884/1‑150 (MQ=255)
                                                                                                                                                cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatta  <  1:556810/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473780‑4474073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: