Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 585,866 G→A 6.1% intergenic (‑233/‑191) ompT ← / → pauD DLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913585,8660GA 6.1% 356.8 / 7.4 113intergenic (‑233/‑191)ompT/pauDDLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER
Reads supporting (aligned to +/- strand):  ref base G (53/53);  new base A (5/2);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

Only 100 of 116 total aligned reads displayed.

TGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAG  >  NC_000913/585717‑586015
                                                                                                                                                     |                                                                                                                                                     
tGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTag                                                                                                                                                       <  1:1344226/150‑1 (MQ=255)
 gTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGATTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTaga                                                                                                                                                      >  2:602974/1‑150 (MQ=255)
    tataTTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATCTTTCGGTTTTCCTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGc                                                                                                                                                   <  2:935008/150‑1 (MQ=255)
    tataTTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGc                                                                                                                                                   <  1:566252/150‑1 (MQ=255)
       atTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCata                                                                                                                                                >  2:1428656/1‑150 (MQ=255)
       atTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCata                                                                                                                                                >  2:1145153/1‑150 (MQ=255)
          ttGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCatggca                                                                                                                                             <  1:1039424/150‑5 (MQ=255)
           tGTTTAGTATAGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTATTATGCTTTTGTGGCGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAg                                                                                                                                            <  2:1471351/150‑1 (MQ=255)
                   aTTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATa                                                                                                                                    >  2:366392/1‑150 (MQ=255)
                   aTTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATa                                                                                                                                    <  1:1644570/150‑1 (MQ=255)
                      gactGATTATCACAATGCGCTATCTGTTTTTGGTTTAAATATCTGTTAATGTTTCATATTTCGGGTGTACTGTGTGGGTTTTGTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCa                                                                                                                                 <  1:110261/147‑1 (MQ=255)
                      gAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCAAATAAGCGAAAATAGCa                                                                                                                                 >  2:763981/1‑150 (MQ=255)
                      gAAAGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTCTCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCa                                                                                                                                 <  2:1080884/150‑1 (MQ=255)
                          gATTATCACAATGCGCTATCTGTTTATGGTTTAATTATCTGTTATTGTTTCATATTACGGTATTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCATTTAGAGGCATATAAGCGAAAATAGCATGAg                                                                                                                             >  1:890897/1‑150 (MQ=255)
                           aTTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTGGAGGCATATAAGCGAAAATAGCATGAgg                                                                                                                            <  1:1912486/150‑1 (MQ=255)
                              aTCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGttt                                                                                                                         >  2:1110391/1‑150 (MQ=255)
                              aTCACAATGCGCTATCTGTTTTTGCTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGAATATAAGCTAAAATAGAATGAGGttt                                                                                                                         >  1:287039/1‑150 (MQ=255)
                               tCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTa                                                                                                                        >  2:253796/1‑150 (MQ=255)
                               tCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTa                                                                                                                        <  2:1027075/150‑1 (MQ=255)
                                cacaATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTAt                                                                                                                       >  2:253055/1‑150 (MQ=255)
                                    aTGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTc                                                                                                                   <  1:1308509/150‑1 (MQ=255)
                                     tGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCa                                                                                                                  >  1:658407/1‑150 (MQ=255)
                                         cTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCATCAATTa                                                                                                              >  1:977052/1‑150 (MQ=255)
                                             cTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATTAGGTTTATCCTCAATTACTAt                                                                                                          >  2:42930/1‑150 (MQ=255)
                                                 ttttGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCTTGAGGTTTATCCTCAATTACTATGttt                                                                                                      >  1:1753043/1‑150 (MQ=255)
                                                    tGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                                   <  2:857274/150‑1 (MQ=255)
                                                     ggTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTa                                                                                                  <  1:925623/150‑1 (MQ=255)
                                                     ggTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGAATATAAGCGAAAATAGAATGAGGTTTATCCTCAATTACTATGTTTTTTa                                                                                                  >  1:1516561/1‑150 (MQ=255)
                                                      gTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAg                                                                                                 >  1:1781955/1‑150 (MQ=255)
                                                      gTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAg                                                                                                 <  1:433302/150‑1 (MQ=255)
                                                      gTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAg                                                                                                 <  2:647007/150‑1 (MQ=255)
                                                       tttAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGt                                                                                                >  2:888395/1‑150 (MQ=255)
                                                       tttAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGt                                                                                                <  2:1359293/150‑1 (MQ=255)
                                                       tttAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATCTATTTTAGt                                                                                                >  2:880388/1‑150 (MQ=255)
                                                        ttAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCATCAATTACTATGTTTTTTAGTa                                                                                               >  1:1179196/1‑150 (MQ=255)
                                                           aTTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaa                                                                                            <  1:735122/150‑1 (MQ=255)
                                                            ttATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaa                                                                                           <  1:52349/150‑1 (MQ=255)
                                                              aTCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaaaa                                                                                         <  1:1890731/150‑1 (MQ=255)
                                                                cTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaaaaat                                                                                       >  2:1754314/1‑149 (MQ=255)
                                                                 tGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                                      >  2:238770/1‑150 (MQ=255)
                                                                  tttATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                     <  1:1741361/149‑1 (MQ=255)
                                                                      ttGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGAc                                                                                 <  2:1665854/150‑1 (MQ=255)
                                                                           tCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGACTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTATTACAAAAAAGAGGGACAAAAc                                                                            <  2:1800724/150‑1 (MQ=255)
                                                                            cATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACt                                                                           >  2:1494845/1‑150 (MQ=255)
                                                                             atatTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTg                                                                          <  2:1564377/150‑1 (MQ=255)
                                                                               atTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTgag                                                                        >  1:1312347/1‑150 (MQ=255)
                                                                               atTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTgag                                                                        >  2:228827/1‑150 (MQ=255)
                                                                                tttCTGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTgaga                                                                       <  2:1118165/150‑1 (MQ=255)
                                                                                tttCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACATAAAAGAGGGACAAAACTgagc                                                                       >  2:1861913/1‑149 (MQ=255)
                                                                                   cGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGacac                                                                    >  2:1753022/1‑150 (MQ=255)
                                                                                    ggTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGAcaca                                                                   <  2:387430/150‑1 (MQ=255)
                                                                                    ggTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGAcaca                                                                   >  2:1288002/1‑150 (MQ=255)
                                                                                    ggTTTTACTGTGTGGTTTTTTTATACTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGAcaca                                                                   >  2:175884/1‑150 (MQ=255)
                                                                                              tgttgtTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCg                                                         <  1:1262649/150‑1 (MQ=255)
                                                                                               gtgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGc                                                        >  2:607098/1‑150 (MQ=255)
                                                                                                tgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACATATAAGGCCTCGCa                                                       >  1:538206/1‑150 (MQ=255)
                                                                                                tgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCATCGCa                                                       >  1:1436547/1‑150 (MQ=255)
                                                                                                cgatctttttATGCTTTTGTGTTGCTTTTATCTATTTAAGTGCCATTCGTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATTTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCa                                                       <  2:1548664/145‑1 (MQ=255)
                                                                                                 ggTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                                      >  1:1322678/1‑150 (MQ=255)
                                                                                                 ggTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                                      >  1:1843624/1‑150 (MQ=255)
                                                                                                  gTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAAt                                                     >  1:1075965/1‑150 (MQ=255)
                                                                                                   tttttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATg                                                    <  1:473815/150‑1 (MQ=255)
                                                                                                    ttttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATgg                                                   <  2:1593009/150‑1 (MQ=255)
                                                                                                     tttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGc                                                  >  1:1758157/1‑150 (MQ=255)
                                                                                                      ttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaagatcg                                                 >  1:1548664/1‑145 (MQ=255)
                                                                                                      ttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCt                                                 >  1:697254/1‑150 (MQ=255)
                                                                                                        ttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTg                                               <  1:763377/150‑1 (MQ=255)
                                                                                                           tGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCaa                                            <  1:1858133/150‑1 (MQ=255)
                                                                                                            gCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAg                                           <  1:1486222/150‑1 (MQ=255)
                                                                                                            gCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAg                                           <  1:1114849/150‑1 (MQ=255)
                                                                                                            gCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATACTCTATTACTATGTTTTTTTGTACAAAAAAGAGGGACAAATCTGAGACACATAAGGCGTCGCAATGGCTTgaatg                                           >  1:1195820/1‑146 (MQ=255)
                                                                                                             cTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAgg                                          >  1:1521859/1‑150 (MQ=255)
                                                                                                             cTTTAGTGGGGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTGTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAACGCCTCGCAATGGCTTGCAAgg                                          <  2:213279/150‑1 (MQ=255)
                                                                                                                ttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCtt                                       <  1:44258/150‑1 (MQ=255)
                                                                                                                ttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCtt                                       <  1:440469/150‑1 (MQ=255)
                                                                                                                  gtggtgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATACAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTa                                     <  1:5912/150‑1 (MQ=255)
                                                                                                                    ggtgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACa                                   >  1:509135/1‑150 (MQ=255)
                                                                                                                       gCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGtt                               >  1:1185949/1‑150 (MQ=255)
                                                                                                                            tATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAATAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGATTGCAAGGATTTACATGTTTTGa                           >  1:255450/1‑150 (MQ=255)
                                                                                                                            tATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGttttta                           >  1:1353767/1‑148 (MQ=255)
                                                                                                                            tATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                           <  2:658407/150‑1 (MQ=255)
                                                                                                                            tATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                           <  1:697326/150‑1 (MQ=255)
                                                                                                                            tATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGATTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                           <  1:280538/150‑1 (MQ=255)
                                                                                                                             aTCTAATTAAGTGCCATGCCTTTAGAGGCATATAACCGAAAATAGCATGAGGTGTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAg                          <  2:289828/150‑1 (MQ=255)
                                                                                                                              tCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAgg                         >  2:1831163/1‑150 (MQ=255)
                                                                                                                                tATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTa                       >  2:47966/1‑150 (MQ=255)
                                                                                                                                    tAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTgg                   >  1:533311/1‑150 (MQ=255)
                                                                                                                                     cgcttataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTggg                  >  2:929308/8‑150 (MQ=255)
                                                                                                                                     aaGTGCCATGCCTTTAGAGGCATATAAGCGAATATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGGCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTggg                  <  2:542642/150‑1 (MQ=255)
                                                                                                                                     aaGAGCCATTACTTTAGATGCATATAAGCGAAAATAGCATGAGGTTTCTCCTCAATTACTATGTTTTTTAGTACAAACAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTggg                  <  2:287039/150‑1 (MQ=255)
                                                                                                                                      gcttataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGa                 >  2:1800653/7‑150 (MQ=255)
                                                                                                                                      aGTGCCATTCCTTTAGATGCATATAAGCGAAAATTGCATGAGTTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGa                 <  2:1268358/150‑1 (MQ=255)
                                                                                                                                       gTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGAc                >  1:1728030/1‑150 (MQ=255)
                                                                                                                                        tGCGATGCATTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCGTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACg               <  2:890897/150‑1 (MQ=255)
                                                                                                                                         gCCATGCATTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgt              >  2:1408291/1‑150 (MQ=255)
                                                                                                                                          ccATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgtg             >  1:885792/1‑150 (MQ=255)
                                                                                                                                            attccTTTAGAGGCATATAAGCGAAAATAGCTTGAGGTTTATCCTCAATTACTATGTTTTTTAGTAGAAACTAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGggtg           <  1:306726/147‑1 (MQ=255)
                                                                                                                                            aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGtgtg           <  2:1841782/150‑1 (MQ=255)
                                                                                                                                             tGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGa          >  1:318774/1‑150 (MQ=255)
                                                                                                                                                  ttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCa     <  1:1460173/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAG  >  NC_000913/585717‑586015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: