Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 603,391 C→G 5.6% intergenic (+56/+25) pheP → / ← ybdG phenylalanine transporter/mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913603,3910CG 5.6% 414.0 / 12.7 138intergenic (+56/+25)pheP/ybdGphenylalanine transporter/mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (56/72);  new base G (6/2);  total (64/74)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 143 total aligned reads displayed.

GTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCAT  >  NC_000913/603242‑603540
                                                                                                                                                     |                                                                                                                                                     
gtgCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGTCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATcc                                                                                                                                                       >  2:402266/1‑150 (MQ=255)
        tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATg                                                                                                                                               <  1:377827/150‑1 (MQ=255)
          gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgag                                                                                                                                             <  2:1101960/150‑1 (MQ=255)
           atgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgaga                                                                                                                                            <  1:691661/150‑1 (MQ=255)
           atgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgaga                                                                                                                                            >  2:1592880/1‑150 (MQ=255)
            tgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGAATTTAAAACGCTGCGTCGGAAATAAGGCATTCACTCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGagag                                                                                                                                           <  2:173355/150‑1 (MQ=255)
             gatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGc                                                                                                                                          <  2:444637/150‑1 (MQ=255)
              atatGCGCTTGTCAGCGATCCTGCTGCCGGTGGGGATTGTATTCCTGTTTATGGAATTAAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACTATGTCAACCATCCGAAACCGCTCACATCCCTTCGATGAGAGCg                                                                                                                                         >  1:1899856/1‑150 (MQ=255)
               tatGCTGTTGTCAGCGTTCCTGCTGACGGTGTGGATTGTATTCATGTTTATTGCATTTAAAACGCTGCGTCGGAATTTAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCgg                                                                                                                                        <  2:349315/150‑1 (MQ=255)
               tatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCgg                                                                                                                                        >  1:1807890/1‑150 (MQ=255)
                    gcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTTATCCATTCGATGAGAGCGGttttt                                                                                                                                   <  2:825514/150‑1 (MQ=255)
                    gcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttt                                                                                                                                   >  1:1376589/1‑150 (MQ=255)
                     cTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  <  2:1477384/150‑1 (MQ=255)
                      ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttttt                                                                                                                                 <  2:560549/150‑1 (MQ=255)
                      ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttttt                                                                                                                                 <  1:479876/150‑1 (MQ=255)
                      ttGGCAGCGATCGTGCTGCAGGTGTGTATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGTAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttttt                                                                                                                                 <  2:150587/150‑1 (MQ=255)
                       tGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTa                                                                                                                                <  2:782462/150‑1 (MQ=255)
                       tGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTa                                                                                                                                <  2:633593/150‑1 (MQ=255)
                          cAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAAtt                                                                                                                             >  2:1547001/1‑150 (MQ=255)
                           aGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTa                                                                                                                            >  1:1590296/1‑150 (MQ=255)
                               aTCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGc                                                                                                                        <  1:1593941/150‑1 (MQ=255)
                                 ccTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTGTAATTACTGCtt                                                                                                                      >  2:107223/1‑150 (MQ=255)
                                 ccTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGATGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAACCGATGTCAACCATCCGAAACCGATAGCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGAtt                                                                                                                      >  2:598039/1‑150 (MQ=255)
                                  ctgctgCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTa                                                                                                                     >  2:748408/1‑150 (MQ=255)
                                   tgctgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTaa                                                                                                                    >  2:1400821/1‑150 (MQ=255)
                                      tgcCGGTGTTGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATg                                                                                                                 <  1:1118903/150‑1 (MQ=255)
                                      tgcCGGTGTTGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATg                                                                                                                 <  2:676526/150‑1 (MQ=255)
                                      ggcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATg                                                                                                                 <  1:1531062/149‑1 (MQ=255)
                                       gcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGc                                                                                                                <  2:37666/150‑1 (MQ=255)
                                       gcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGc                                                                                                                >  2:1355693/1‑150 (MQ=255)
                                          ggTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAATCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCAcc                                                                                                             >  2:1892447/1‑150 (MQ=255)
                                             gtgGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGc                                                                                                          <  2:1487571/150‑1 (MQ=255)
                                                 aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTGCATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCaga                                                                                                      <  1:517096/150‑1 (MQ=255)
                                                  ttGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagag                                                                                                     <  1:1099081/150‑1 (MQ=255)
                                                  ttGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATACACGCTACATCCGACAAAACGATGGCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagag                                                                                                     <  2:796235/150‑1 (MQ=255)
                                                    gTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCagagag                                                                                                   >  2:1512483/1‑150 (MQ=255)
                                                        tCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGaa                                                                                               <  1:289488/150‑1 (MQ=255)
                                                         ccTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAat                                                                                              <  1:1300355/150‑1 (MQ=255)
                                                           tGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAatat                                                                                            <  2:991425/150‑1 (MQ=255)
                                                           tGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAata                                                                                             >  1:71085/1‑149 (MQ=255)
                                                               tATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                        >  1:1634893/1‑150 (MQ=255)
                                                               tATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                        <  2:1903923/150‑1 (MQ=255)
                                                                aTGGCATTTATACCGCTGCGTCGTAAATAAGTCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTg                                                                                       <  2:1223630/150‑1 (MQ=255)
                                                                aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGTGCGAATATCATTg                                                                                       >  1:783374/1‑150 (MQ=255)
                                                                aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTg                                                                                       >  2:1859972/1‑150 (MQ=255)
                                                                aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTg                                                                                       >  2:468818/1‑150 (MQ=255)
                                                                 tGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCACGCATCCGAAACCGCTCTCTTCGATATGAGGAGAGCGGTGTTTTTAATTACTGCTTTTATGCACCAGCCAGAGAGCGATTCTGATTGc                                                                                      >  2:1655923/1‑150 (MQ=255)
                                                                   gCATTTAAAACGCTGCGTCGGAAATAAGGCATTCCCGCTACATCCGACTAAGCGATGTCAACCATCCGAAACCGCTCTCAGGCAGTCGATGAGAGCGGTTTTTTTACTTACTGCTTAAATGCACCCGCCAGAGAGAGCATTTGATTGCCg                                                                                    >  2:307271/1‑150 (MQ=255)
                                                                    cATTTAAAACGCTGCGTCGGAAATAAGTCATTCAAGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCACATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCgg                                                                                   <  2:909076/150‑1 (MQ=255)
                                                                            aCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGAc                                                                           <  2:1036730/150‑1 (MQ=255)
                                                                            aCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGAc                                                                           <  2:893160/150‑1 (MQ=255)
                                                                             cGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACt                                                                          <  1:1704615/150‑1 (MQ=255)
                                                                              gCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtg                                                                         <  1:1781789/150‑1 (MQ=255)
                                                                                 gagTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtgatg                                                                      <  1:143667/148‑1 (MQ=255)
                                                                                      ggAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGCGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTc                                                                 >  2:1228363/1‑150 (MQ=255)
                                                                                      ggAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTc                                                                 >  2:100411/1‑150 (MQ=255)
                                                                                       gAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAGATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCg                                                                <  2:1328758/150‑1 (MQ=255)
                                                                                       gAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCg                                                                >  2:1712999/1‑150 (MQ=255)
                                                                                        aaaTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGc                                                               <  1:1400821/150‑1 (MQ=255)
                                                                                         aaTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                              <  1:1331366/150‑1 (MQ=255)
                                                                                           tAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGTTTTTTTTAATTAATGCTAAAATGCACCCGCCAGACATCTAATATCATTGCAGGTTGGCGACTGATGAAGTccatga                                                            >  1:378017/1‑145 (MQ=255)
                                                                                            aaGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGAc                                                           <  2:487915/150‑1 (MQ=255)
                                                                                               gCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaa                                                        <  2:1698630/150‑1 (MQ=255)
                                                                                                cATTCACGCTACATCCGACAAGACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaaa                                                       >  1:862858/1‑150 (MQ=255)
                                                                                                cATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaaa                                                       >  2:722656/1‑150 (MQ=255)
                                                                                                cATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaaa                                                       <  2:1807890/150‑1 (MQ=255)
                                                                                                 aTTCACCCGACATCCGACAAAACGAAGTCAACCCTCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAAc                                                      <  2:1175739/150‑1 (MQ=255)
                                                                                                     aCGCTACTACCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTAAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCtt                                                  <  1:364112/150‑1 (MQ=255)
                                                                                                      cGCTACATCCGTCAAAAATATGTCAACCATCGGAAACAGGTCTCATCCATTCGATGATAGCGGTTTTTTTAATTACTGGTTAAATGCACCCGCCAGATATCGAATATCTTTGCCGGTTGGATAATTATGAAGGCGCAGACCAAACTCTTc                                                 <  2:212145/150‑1 (MQ=255)
                                                                                                      cGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc                                                 <  1:1021812/150‑1 (MQ=255)
                                                                                                       gCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCg                                                <  2:1736324/150‑1 (MQ=255)
                                                                                                            aTCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAAt                                           <  2:1477272/150‑1 (MQ=255)
                                                                                                              ccGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCg                                         >  1:785814/1‑150 (MQ=255)
                                                                                                              ccGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAATCGATGAGAGCGGTTTTTTTAATTACTGCTTTAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCg                                         <  1:1347682/150‑1 (MQ=255)
                                                                                                               cGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGc                                        <  2:1426917/150‑1 (MQ=255)
                                                                                                                  cAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCTATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAAGATCATTGCCGGTTGGCGCCTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCaaa                                     >  1:1208181/1‑150 (MQ=255)
                                                                                                                      aCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtg                                 <  2:594860/150‑1 (MQ=255)
                                                                                                                           gTCAACCATCCTAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATTCACACGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATc                            <  1:1807411/150‑1 (MQ=255)
                                                                                                                           gTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATc                            >  1:1471456/1‑150 (MQ=255)
                                                                                                                             cAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGa                          >  2:193557/1‑150 (MQ=255)
                                                                                                                              aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGaa                         <  1:621651/150‑1 (MQ=255)
                                                                                                                                ccATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       >  1:1362908/1‑150 (MQ=255)
                                                                                                                                ccATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAAAATGTGATCGAata                       <  1:754610/150‑1 (MQ=255)
                                                                                                                                     cGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTAGACAATCGCAAATATGTGATCGAATATATCa                  >  1:1820240/1‑150 (MQ=255)
                                                                                                                                      gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAg                 <  2:932576/150‑1 (MQ=255)
                                                                                                                                       aaaCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGATTATCATTGCGGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGc                >  1:42909/1‑150 (MQ=255)
                                                                                                                                         aCCGCTCTCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCt              >  1:575043/1‑150 (MQ=255)
                                                                                                                                         aCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCt              <  1:207709/150‑1 (MQ=255)
                                                                                                                                           cGCTCTCATCGCATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAAGATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa            >  1:435851/1‑150 (MQ=255)
                                                                                                                                            gCTCTCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGaa           >  1:1257238/1‑150 (MQ=255)
                                                                                                                                             ctctCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAGTATGTGATCGAATATATCAGCCTGAAt          <  1:1512483/150‑1 (MQ=255)
                                                                                                                                              tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGATCGAATATCATTGCCGGTTGTCTACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATg         <  1:88274/150‑1 (MQ=255)
                                                                                                                                               ctcATCCATTCGATGAGAGCGGTTTTTTTCATTACTGCTTCAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAAAGc        >  2:726816/1‑150 (MQ=255)
                                                                                                                                                 cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCtt      >  2:457326/1‑150 (MQ=255)
                                                                                                                                                 cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCtt      >  2:350744/1‑150 (MQ=255)
                                                                                                                                                 cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCtt      >  2:1276684/1‑150 (MQ=255)
                                                                                                                                                 cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCACAAATATGTGATCGAATATATCAGCCTGAATGCtt      >  1:1211909/1‑150 (MQ=255)
                                                                                                                                                  aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCttt     <  2:875008/150‑1 (MQ=255)
                                                                                                                                                  aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCttt     >  2:406666/1‑150 (MQ=255)
                                                                                                                                                     cATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat  <  1:1495212/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCAT  >  NC_000913/603242‑603540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: