Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 641,360 C→T 5.2% intergenic (+42/+79) ahpF → / ← uspG alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913641,3600CT 5.2% 369.9 / 4.2 113intergenic (+42/+79)ahpF/uspGalkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12
Reads supporting (aligned to +/- strand):  ref base C (58/49);  new base T (3/3);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CTGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGG  >  NC_000913/641216‑641509
                                                                                                                                                |                                                                                                                                                     
cTGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGATGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAAcc                                                                                                                                                  >  1:442181/1‑150 (MQ=255)
    acgccgGTTCCGTACCAGCCGATCATCATCGCCACAGGCGAAGGTGCCAAAGCCTCTCTGAGGGCGTTTGACTACCTGCTTCGCACCAAACCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                              <  1:170556/150‑1 (MQ=255)
      gacgGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggcgg                                                                                                                                            >  2:1400726/1‑150 (MQ=255)
       acgGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggcggc                                                                                                                                           >  1:1596541/1‑150 (MQ=255)
       acgGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCAGGTTTGACCCCggcggc                                                                                                                                           <  1:1321663/150‑1 (MQ=255)
        cgGTTCCGTACAAGCAGCTCATCATCGCCCCTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGACGAAGTAAGATTCACCTGCAAGTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                          <  1:1023189/150‑1 (MQ=255)
        cgGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                          <  1:1875744/150‑1 (MQ=255)
        cgGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                          >  1:1735495/1‑150 (MQ=255)
        cgGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCAGGTTTGACCCCGGCGGCt                                                                                                                                          <  2:889161/150‑1 (MQ=255)
          gTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCttt                                                                                                                                        >  2:913012/1‑150 (MQ=255)
           ttCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCtttt                                                                                                                                       <  2:1520919/150‑1 (MQ=255)
              cGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCttttttt                                                                                                                                    >  1:347688/1‑149 (MQ=255)
              cGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                    >  2:1304467/1‑150 (MQ=255)
               gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTAt                                                                                                                                   <  1:837401/150‑1 (MQ=255)
                tACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATg                                                                                                                                  >  1:1521339/1‑150 (MQ=255)
                tACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATg                                                                                                                                  >  2:555079/1‑150 (MQ=255)
                  cAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGc                                                                                                                                >  1:419801/1‑150 (MQ=255)
                  cAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGc                                                                                                                                >  2:536821/1‑150 (MQ=255)
                  cAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAACCTGGCGGGGTTTTTATGGCa                                                                                                                               >  1:1660505/1‑150 (MQ=255)
                    aGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAt                                                                                                                              >  1:1455737/1‑150 (MQ=255)
                    aGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTAGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAt                                                                                                                              <  2:1264058/150‑1 (MQ=255)
                          tcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGCCTACCTGATTCGCACCAAAACTGCATAAGAAGAAGAAAGATTCACCTGCAATTGCTAAGCCGCCGGGGTCAAACCTGGCGGCTTGTTAATGGCATTaaaaa                                                                                                                        >  2:939865/1‑150 (MQ=255)
                           catcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAg                                                                                                                       >  2:1908279/1‑150 (MQ=255)
                           catcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAg                                                                                                                       >  1:1370777/1‑150 (MQ=255)
                           caccttcGCCAGTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAg                                                                                                                       >  1:150911/6‑150 (MQ=255)
                              catcGCCACTGGCGAAGGTGCCAAAGCCTCTATGAGTGCTGTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCCCCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGATTTTTTATGGAATTAAAAAGccc                                                                                                                    >  1:1521366/1‑150 (MQ=255)
                                  gCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgcc                                                                                                                <  2:506109/150‑1 (MQ=255)
                                       tGGCGAAGGTGCCAACGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTATCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAATCCCCGCCGGGAt                                                                                                           >  1:1171422/1‑150 (MQ=255)
                                        ggCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATg                                                                                                          <  2:1254500/150‑1 (MQ=255)
                                        ggCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACATGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATg                                                                                                          >  2:505715/1‑150 (MQ=255)
                                          cGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGc                                                                                                        <  2:1564903/150‑1 (MQ=255)
                                             aGGTGCCAAAGCCTCTCAGAGTGCTTTTGACTACCTGATTCGCACCAACACTGCATAAGACGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTcc                                                                                                     <  1:399063/150‑1 (MQ=255)
                                              ggTGCCAAAGCCTCTCTGAGTGCTTTTGCCTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCg                                                                                                    >  1:743395/1‑150 (MQ=255)
                                              ggTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCg                                                                                                    >  1:1495812/1‑150 (MQ=255)
                                                tGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCggg                                                                                                  >  1:757525/1‑150 (MQ=255)
                                                   cAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                               >  2:1543206/1‑150 (MQ=255)
                                                   cAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                               >  1:1767972/1‑150 (MQ=255)
                                                    aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAgg                                                                                              <  2:1887227/150‑1 (MQ=255)
                                                     aaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAggg                                                                                             <  1:410635/150‑1 (MQ=255)
                                                        ccTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCgg                                                                                          <  2:1520097/150‑1 (MQ=255)
                                                          tctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                        <  2:1452459/150‑1 (MQ=255)
                                                           ctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGAt                                                                                       >  1:834763/1‑150 (MQ=255)
                                                             ctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCTGCTTATTTATGGCATTAAAAACCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                     >  1:345417/1‑150 (MQ=255)
                                                             ctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                     <  1:787108/150‑1 (MQ=255)
                                                               taGTGCTTTTGACAACCATAATCGACCCAAAAGTGCATAAGAAGAAGTAACATTGACCTGCAATTGCTTACCCGCCGGGCTTAAACCTCGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCTCGCAGGGCGGGATACtt                                                                                   <  2:941459/149‑1 (MQ=255)
                                                               gAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACtt                                                                                   <  1:1847937/150‑1 (MQ=255)
                                                                     ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGCTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTg                                                                             <  2:1481783/150‑1 (MQ=255)
                                                                     ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTg                                                                             <  2:1138647/150‑1 (MQ=255)
                                                                       ttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGc                                                                           <  2:616391/150‑1 (MQ=255)
                                                                        tGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCa                                                                          <  2:386921/150‑1 (MQ=255)
                                                                        tGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCa                                                                          <  1:1218795/150‑1 (MQ=255)
                                                                         gACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCaa                                                                         >  1:596059/1‑150 (MQ=255)
                                                                         gACTACCTGATTCCCACCAAAACTGCATCAGAACAAGAAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCaa                                                                         <  1:62237/150‑1 (MQ=255)
                                                                            tACCTGATTCGCACCAATTCTGCATAAGAAGTAGTATGATTCAGCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTa                                                                      <  2:931632/150‑1 (MQ=255)
                                                                             aCCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTac                                                                     >  1:70305/1‑149 (MQ=255)
                                                                                   ttCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGcaca                                                               >  1:1403987/1‑150 (MQ=255)
                                                                                   ttCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGcaca                                                               <  1:934366/150‑1 (MQ=255)
                                                                                      gCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAcc                                                            <  1:848367/150‑1 (MQ=255)
                                                                                       cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCa                                                           >  2:1332154/1‑150 (MQ=255)
                                                                                        aCCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAg                                                          >  2:1054668/1‑150 (MQ=255)
                                                                                            aaaCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCAcc                                                      <  2:406430/150‑1 (MQ=255)
                                                                                              aCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                    <  1:913012/150‑1 (MQ=255)
                                                                                               cTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGc                                                   <  2:195741/150‑1 (MQ=255)
                                                                                                tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCa                                                  <  1:1029333/150‑1 (MQ=255)
                                                                                                  cATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCATGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGa                                                >  1:1165048/1‑150 (MQ=255)
                                                                                                  cATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCCCCGGCAGa                                                >  1:1559082/1‑150 (MQ=255)
                                                                                                   aTAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGAt                                               >  2:619801/1‑150 (MQ=255)
                                                                                                       gaagaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCGTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGTATACTTATTCTGGCAATTAACGCACAACCCGCACCGGCAGATtggc                                           >  2:846193/1‑150 (MQ=255)
                                                                                                          gaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtg                                        >  2:1712930/1‑150 (MQ=255)
                                                                                                             gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggcg                                     >  1:701794/1‑150 (MQ=255)
                                                                                                             gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggca                                     <  2:1477329/150‑2 (MQ=255)
                                                                                                              tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGTGCAGGGCGGGCTACTTATTCTGGCAATTAACGCACAACCAGCCACGGCAGAGTGGCGTGGCgg                                    >  2:1730867/1‑150 (MQ=255)
                                                                                                              tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCgg                                    <  2:524721/150‑1 (MQ=255)
                                                                                                               aaGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAATAAAAAGCCCCGCCGGGGTGGCTCCGGGCAGGGCGGGATACTTATTATGGCAAATAACGCACAACCAGCACCGGCAGAATGGCGTGGCggt                                   >  1:1563710/1‑149 (MQ=255)
                                                                                                                aGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATTGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGCTTGGCGTGGCGGAt                                  >  1:763013/1‑150 (MQ=255)
                                                                                                                aGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGAt                                  <  2:345417/150‑1 (MQ=255)
                                                                                                                aGATTCACCTGCAATTGCTTAGCCACCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCGCCGGCAGATTGGCGTGGCGGAt                                  >  1:200851/1‑150 (MQ=255)
                                                                                                                    tCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACg                              <  2:757525/150‑1 (MQ=255)
                                                                                                                    tCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAGTAAAAAGCCCCGCCGGGAGGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCTCATCCAGCACCGGCAGATTGGCGGGGCGGATCACg                              <  1:46452/150‑1 (MQ=255)
                                                                                                                      aCCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAGGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACCACCAGCCCCGGCAGATTGGCGTGGCGGATCACGcg                            >  1:1529231/1‑149 (MQ=255)
                                                                                                                          gCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAg                        >  2:1704185/1‑150 (MQ=255)
                                                                                                                          gCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCGCGAg                        >  1:683290/1‑150 (MQ=255)
                                                                                                                               tGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGtt                   <  2:932957/150‑1 (MQ=255)
                                                                                                                                gCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                  <  2:1370777/150‑1 (MQ=255)
                                                                                                                                gCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                  <  2:596059/150‑1 (MQ=255)
                                                                                                                                gCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                  >  2:559021/1‑150 (MQ=255)
                                                                                                                                  ttAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGa                >  1:871380/1‑150 (MQ=255)
                                                                                                                                   aaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGCTTGGCGTGGCGGATCACGCTCGAGGCGTTAGaa               >  1:527972/2‑150 (MQ=255)
                                                                                                                                    aGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAc              >  2:1847558/1‑150 (MQ=255)
                                                                                                                                     gccgccGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc             >  1:1801398/1‑150 (MQ=255)
                                                                                                                                     gccgccGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc             >  2:478643/1‑150 (MQ=255)
                                                                                                                                     gccgccGGGGGCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc             <  2:936000/150‑1 (MQ=255)
                                                                                                                                      ccgccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTTTTCTGGCAATTAACGCACCACCAGCACCGGCAGATTGGCGTGGCGGATCACGCGCGAGGCGTTAGAAccg            >  1:1872957/1‑149 (MQ=255)
                                                                                                                                      ccgccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCt            <  1:870747/150‑1 (MQ=255)
                                                                                                                                        gccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGGGGCGGATCACGCACGAGGCGATAGAACCGaa          >  2:320277/1‑150 (MQ=255)
                                                                                                                                          cgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACa        <  2:1664604/150‑1 (MQ=255)
                                                                                                                                              ggcAAACCTGGCGGCATTTTTATGGCAATAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGCATACTTAGTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCGCGAGGCGTTAGAACCTAACAGATg    <  2:101469/148‑1 (MQ=255)
                                                                                                                                              gTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGAGGGCTCCGGGCAGGGCGGGATACTTATTCAGGCAATTAACGCACAACCACCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGACCCTAACAGATg    <  2:621006/150‑1 (MQ=255)
                                                                                                                                               tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATgg   >  2:1711400/1‑150 (MQ=255)
                                                                                                                                                cAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATggg  >  1:1849833/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGG  >  NC_000913/641216‑641509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: