Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 645,073 T→C 6.5% intergenic (‑70/+44) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913645,0730TC 6.5% 371.0 / 12.0 123intergenic (‑70/+44)rna/citTribonuclease I/citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base T (46/69);  new base C (2/6);  total (48/75)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

Only 100 of 126 total aligned reads displayed.

TGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATA  >  NC_000913/644924‑645221
                                                                                                                                                     |                                                                                                                                                    
tGCAACGCTAAGGCGTTGGAAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGTCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCt                                                                                                                                                      <  2:507947/150‑1 (MQ=255)
 gCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCtt                                                                                                                                                     >  2:1178549/1‑150 (MQ=255)
    aCGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTAcc                                                                                                                                                  <  2:1547510/150‑1 (MQ=255)
    aCGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTAcc                                                                                                                                                  <  1:651168/150‑1 (MQ=255)
    aCGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTAcc                                                                                                                                                  >  1:1587585/1‑150 (MQ=255)
     cGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCt                                                                                                                                                 >  1:969546/1‑150 (MQ=255)
     cGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCt                                                                                                                                                 >  1:1635319/1‑150 (MQ=255)
      gCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGCCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTg                                                                                                                                                >  2:911169/1‑150 (MQ=255)
       cTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTgg                                                                                                                                               >  1:1394495/1‑150 (MQ=255)
         aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                             <  2:1873832/150‑1 (MQ=255)
          aGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgg                                                                                                                                            >  2:654337/1‑150 (MQ=255)
          aGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgg                                                                                                                                            <  1:132403/150‑1 (MQ=255)
           ggCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcggg                                                                                                                                           <  1:244166/150‑1 (MQ=255)
           ggCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGGGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcggg                                                                                                                                           <  2:392153/150‑1 (MQ=255)
           cgCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGCGCCACGCGGCGGTACGCCAGACTGCTTGCATACTGGGTGGGGAACGCATACCTCCACTGAATACTATCTATTAACTCCTAAAACAGCCCGCCAGGTGGGCTTACCTggcggg                                                                                                                                           <  1:1169190/149‑1 (MQ=255)
              gTTGGCAGACGACAAGGGAAGCAGAGAACCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATCCTTACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGt                                                                                                                                        <  1:1473755/150‑1 (MQ=255)
                 ggCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAAAGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGtgat                                                                                                                                     >  1:1376297/1‑150 (MQ=255)
                   cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcgggctttttta                                                                                                                                   <  1:1105386/150‑8 (MQ=255)
                   cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcgggctttttta                                                                                                                                   <  2:771810/150‑8 (MQ=255)
                    agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTgatgat                                                                                                                                  <  2:996977/150‑1 (MQ=255)
                    agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTgatgat                                                                                                                                  >  2:895070/1‑150 (MQ=255)
                    agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATAAACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTgatgat                                                                                                                                  <  2:1797365/150‑1 (MQ=255)
                    agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATCCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCAAAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTgatgat                                                                                                                                  <  1:125491/150‑1 (MQ=255)
                           aagGGCAGCAGAGAACCCGCGAGCAACGCGGCGTTACGCCAGACTGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCa                                                                                                                           <  1:1699164/150‑1 (MQ=255)
                            agGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATTCTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAg                                                                                                                          <  2:659107/150‑1 (MQ=255)
                             gggAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGCCTTACCTGGCGGGCGTGATGATTTATTCacc                                                                                                                         >  2:728571/1‑148 (MQ=255)
                               gAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGt                                                                                                                       <  2:1656975/150‑1 (MQ=255)
                                  gCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTg                                                                                                                    >  1:1159541/1‑150 (MQ=255)
                                    agagAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGc                                                                                                                  <  2:456384/150‑1 (MQ=255)
                                     gagaAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCg                                                                                                                 >  1:662588/1‑150 (MQ=255)
                                        aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                              <  1:541251/150‑1 (MQ=255)
                                         aaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACg                                                                                                             <  2:1320850/150‑1 (MQ=255)
                                         aaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACg                                                                                                             <  1:1081825/150‑1 (MQ=255)
                                         aaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACg                                                                                                             <  2:1636312/150‑1 (MQ=255)
                                           ccGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTa                                                                                                           <  1:1823361/150‑1 (MQ=255)
                                             gcgAGCAACGGGGGGTTACGCCAGAATGCTTTGATAATGGGGGTGGATCTCATACATACACTGAATACTATCTATTAACTCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                         <  2:1618672/150‑1 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                         <  1:1463974/150‑1 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                         <  2:865864/150‑1 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                         <  2:1613204/150‑1 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACAAGCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                         <  2:1256773/150‑1 (MQ=255)
                                              cgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACTCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTa                                                                                                        <  1:242765/150‑1 (MQ=255)
                                                aGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGTCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGt                                                                                                      >  1:1536470/1‑150 (MQ=255)
                                                aGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGt                                                                                                      >  2:191788/1‑150 (MQ=255)
                                                aGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGt                                                                                                      >  2:1037392/1‑150 (MQ=255)
                                                aGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGt                                                                                                      >  2:1123897/1‑150 (MQ=255)
                                                  cAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACCTAATAGtta                                                                                                    >  2:1551236/1‑149 (MQ=255)
                                                      gcggcgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCcaca                                                                                                <  2:970404/150‑1 (MQ=255)
                                                         gcgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATgg                                                                                             <  1:52073/150‑1 (MQ=255)
                                                         gcgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATgg                                                                                             <  1:1043162/150‑1 (MQ=255)
                                                            ttACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                          <  2:186634/150‑1 (MQ=255)
                                                             tACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCgag                                                                                         >  1:1652064/1‑150 (MQ=255)
                                                              aCGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCCCATGGCgaga                                                                                        >  2:1630365/1‑150 (MQ=255)
                                                                   aGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGGGTGATGATTTATTCAGAGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATAgg                                                                                   >  2:64463/1‑150 (MQ=255)
                                                                   aGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCgg                                                                                   >  1:649985/1‑150 (MQ=255)
                                                                     aaTGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgcc                                                                                 >  2:581009/1‑150 (MQ=255)
                                                                       tGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                               <  2:842273/150‑1 (MQ=255)
                                                                       tGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                               <  1:1713824/150‑1 (MQ=255)
                                                                       tGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                               <  1:584566/150‑1 (MQ=255)
                                                                       tGCTTTCATAATGGGGGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                               <  1:814174/150‑1 (MQ=255)
                                                                         cTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcc                                                                             >  1:785859/1‑150 (MQ=255)
                                                                         cTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcc                                                                             <  1:370995/150‑1 (MQ=255)
                                                                         cTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcc                                                                             >  1:1750469/1‑150 (MQ=255)
                                                                         cTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCAAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcc                                                                             >  1:536587/1‑150 (MQ=255)
                                                                              aTAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATAATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTATGGCGAACGTATTAGTTCCACATGGCGAGAATCTGCCAGcaaacca                                                                        >  1:1203756/1‑150 (MQ=255)
                                                                                  tGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAg                                                                    <  1:222016/150‑1 (MQ=255)
                                                                                       gtgGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGGGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACa                                                               <  1:1665042/150‑1 (MQ=255)
                                                                                        tgGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACAt                                                              <  2:1635319/150‑1 (MQ=255)
                                                                                         ggAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATa                                                             >  2:882845/1‑150 (MQ=255)
                                                                                         ggAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCAGGCGGGCGTGATGATTTATTCAGCGTTTGTCGAACGTATTAGTTCCACAAGGCGAGAATCGGCCAGCCAACCAACAGCAACATa                                                             >  1:677565/1‑150 (MQ=255)
                                                                                             cTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAgaga                                                         <  2:1414574/150‑1 (MQ=255)
                                                                                               catacataCACTGAATACTATCTAATAAATCATAAAAAAGTCCGCCCGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATg                                                       <  2:939971/150‑1 (MQ=255)
                                                                                                atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                      <  2:1776726/150‑1 (MQ=255)
                                                                                                 tacatacaCTGATTACTTTCTATTAATTCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTa                                                     <  1:360862/150‑1 (MQ=255)
                                                                                                        acTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcc                                              >  2:1579715/1‑150 (MQ=255)
                                                                                                           gAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCg                                           <  2:407750/150‑1 (MQ=255)
                                                                                                                   tctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgc                                   >  2:1853174/1‑150 (MQ=255)
                                                                                                                   tctatAAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgc                                   <  2:395455/150‑1 (MQ=255)
                                                                                                                     tatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCACCCAACAGCAACATAGACATGTAAATCACCCCGATGATTgcgc                                 >  2:1632584/1‑150 (MQ=255)
                                                                                                                     tatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGGTTGGCGAACGGATTAGTTCCACAGGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAAGCCCCCCGAAGATTgcgc                                 >  1:947704/1‑150 (MQ=255)
                                                                                                                      atTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGcc                                <  1:9158/150‑1 (MQ=255)
                                                                                                                           aTCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGAc                           >  1:422116/1‑150 (MQ=255)
                                                                                                                             cATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGc                         >  2:1035681/1‑150 (MQ=255)
                                                                                                                             cATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGc                         >  2:1714156/1‑150 (MQ=255)
                                                                                                                              aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCATCATAGAGATGTAAATCCCCCCGATGATTGCGCCAAGACGcc                        >  1:363308/1‑150 (MQ=255)
                                                                                                                              aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGcc                        <  2:645840/150‑1 (MQ=255)
                                                                                                                              aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGcc                        >  1:1069540/1‑150 (MQ=255)
                                                                                                                              aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGcc                        <  2:1687684/150‑1 (MQ=255)
                                                                                                                              aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGcc                        >  2:1600997/1‑150 (MQ=255)
                                                                                                                               aaaaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                       <  2:277947/149‑1 (MQ=255)
                                                                                                                                 aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                     <  1:1206448/150‑1 (MQ=255)
                                                                                                                                    aaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAAACGGCCAGCCAACCAACAGCAAAATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGCCGCCAGTAAt                  <  1:941559/150‑1 (MQ=255)
                                                                                                                                     cgCCCGCCAGGTAAGCCCACCTGGCGGGCGTGATGATTTAATCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGATTCGGCCAGCCACCCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGCCGACAGTAAta                 >  1:1450271/2‑149 (MQ=255)
                                                                                                                                          gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTg            <  2:144683/150‑1 (MQ=255)
                                                                                                                                          gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTg            <  2:1139273/150‑1 (MQ=255)
                                                                                                                                           ccAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGa           >  2:1375600/1‑150 (MQ=255)
                                                                                                                                             agggtggCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCTAACGTATTAGTTCCACATGGCGACAATCGGACAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAtt         <  1:64463/145‑1 (MQ=255)
                                                                                                                                               gTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc       <  2:1536470/150‑1 (MQ=255)
                                                                                                                                                  ggCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTcaca    <  1:669629/150‑1 (MQ=255)
                                                                                                                                                   gCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAt   <  2:873993/150‑1 (MQ=255)
                                                                                                                                                   gCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAt   <  1:998831/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACATA  >  NC_000913/644924‑645221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: