Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,376 G→T 8.1% intergenic (+36/‑207) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3760GT 8.1% 371.3 / 16.9 138intergenic (+36/‑207)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base G (69/57);  new base T (4/8);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 142 total aligned reads displayed.

TCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAA  >  NC_000913/729228‑729525
                                                                                                                                                    |                                                                                                                                                     
  tGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGaaa                                                                                                                                                    >  1:1785370/1‑150 (MQ=255)
   ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                   >  2:103093/1‑150 (MQ=255)
        gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                              >  2:1833172/1‑150 (MQ=255)
        gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                              <  1:1520986/150‑1 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                            >  2:981706/1‑150 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                            <  1:698252/150‑1 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTAGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                            <  1:400774/150‑1 (MQ=255)
           cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCgg                                                                                                                                           <  2:1033331/150‑1 (MQ=255)
            gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGt                                                                                                                                          <  2:444109/150‑1 (MQ=255)
            gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGt                                                                                                                                          <  2:437000/150‑1 (MQ=255)
             cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACAAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                         <  2:1451538/150‑1 (MQ=255)
               cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttt                                                                                                                                       >  1:1166872/1‑150 (MQ=255)
                ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                      >  2:7036/1‑150 (MQ=255)
                ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                      >  2:1114915/1‑150 (MQ=255)
                 cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                                                     <  2:1781020/150‑1 (MQ=255)
                  gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                                                    <  2:656145/150‑1 (MQ=255)
                  gCTTTTACAGCTACCTGCATCGCGGCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                                                    <  1:1617312/150‑1 (MQ=255)
                       tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCa                                                                                                                               >  1:299620/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCTTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                                             <  2:808949/150‑1 (MQ=255)
                           gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGaa                                                                                                                           >  1:581496/1‑150 (MQ=255)
                            cTACCTGCATCGCGTCTGGTCGAAAAGCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                                          <  2:15069/150‑1 (MQ=255)
                             tACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGa                                                                                                                         >  2:493262/1‑150 (MQ=255)
                               ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCAGGAAGAtt                                                                                                                       >  1:1798498/1‑149 (MQ=255)
                                cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                                                      >  2:1117966/1‑150 (MQ=255)
                                 tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                                                     >  2:399682/1‑150 (MQ=255)
                                  gCATCGCGTCTGGTCGAAATCCATCGATAAACCGGTGTGGATGGATCACGCCGAAAAGGCAACTGGTGATTTTTATTGATTTCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                    <  2:1332908/150‑1 (MQ=255)
                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                    <  1:1547714/150‑1 (MQ=255)
                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                    <  2:943732/150‑1 (MQ=255)
                                     tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAACCGCTGGCGGTTATTTGTATCAGGAAGATGTTCaga                                                                                                                 >  2:887652/1‑150 (MQ=255)
                                              gccGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                        <  2:735596/148‑1 (MQ=255)
                                               tCGAAAACCAGCGATTAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCTCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                       <  2:1807459/150‑1 (MQ=255)
                                               tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                       >  1:470624/1‑150 (MQ=255)
                                                cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                      <  1:1101264/150‑1 (MQ=255)
                                                      ccAGCGATAAACCTGTGTGGATGGATCAGGCCGAAAAGGCAACTGGCGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                                                <  1:1144379/150‑1 (MQ=255)
                                                       cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                               >  1:777774/1‑150 (MQ=255)
                                                         gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                             <  2:543562/150‑1 (MQ=255)
                                                          cGATAAACCTGGGTGGTTGGATCTGGCCGAAAATGCTACTTGTGATTTTTATTGATTGCTGTGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtat                                                                                            <  1:201483/150‑1 (MQ=255)
                                                           gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGTAAAATGTTCAGAGGAAAATTTAGACATCGTtata                                                                                           >  1:318987/1‑150 (MQ=255)
                                                            aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAc                                                                                          >  1:162199/1‑150 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                         <  1:1127723/150‑1 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                         >  1:10083/1‑150 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                         >  2:1175647/1‑150 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                         <  2:1346502/150‑1 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                         >  1:413381/1‑150 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                         >  1:490926/1‑150 (MQ=255)
                                                              aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGTCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCa                                                                                        >  2:1208841/1‑150 (MQ=255)
                                                                 ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATc                                                                                     <  1:1284457/150‑1 (MQ=255)
                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                   <  2:1627091/150‑1 (MQ=255)
                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                   >  1:1009646/1‑150 (MQ=255)
                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                   >  1:605317/1‑150 (MQ=255)
                                                                    gtgtGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaa                                                                                  <  1:536847/150‑1 (MQ=255)
                                                                              atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAtta                                                                        >  1:890177/1‑149 (MQ=255)
                                                                              atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTc                                                                        >  2:1815032/1‑150 (MQ=255)
                                                                               tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                                       >  2:436681/1‑150 (MQ=255)
                                                                                 attccGAATAGGCAAATTGTGTTTTTTTATGATTTTTGAGTTTTTAATAAATTGTTAACCGCCAGATGAATAGCTGGCTGTTTTTTGAATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAATCTTATATTCATg                                                                     <  1:69779/147‑1 (MQ=255)
                                                                                  ggCGGAAATGGCATCTGGTGAGTTTTCTTGAGTGCTGAGTTTTGAGGACGTTGAAAACCGCCAGCGGAACGGCTGGCGGTTTTTTGTATCAGGAAGATGTTGAGAGGTATATTTAGACATCGTTATAGCATTCGAATCATATATTCATGa                                                                    >  2:288624/1‑150 (MQ=255)
                                                                                  ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGa                                                                    <  2:1618648/150‑1 (MQ=255)
                                                                                    cctaaaaGTCAACTTGTGATTTTTATTGATTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaaa                                                                  <  1:184142/147‑1 (MQ=255)
                                                                                    ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaaa                                                                  <  2:1555121/150‑1 (MQ=255)
                                                                                     cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGGTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAat                                                                 >  2:851929/1‑150 (MQ=255)
                                                                                      gAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAata                                                                >  2:1496123/1‑150 (MQ=255)
                                                                                        aaaGGCAACTGGTGATTTTTATTGATAGCGGAGTATTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAACATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATACATCAAaaata                                                              >  1:1270442/1‑150 (MQ=255)
                                                                                          aGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatata                                                            <  1:1320216/150‑1 (MQ=255)
                                                                                                  ggTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtt                                                    >  2:410709/1‑150 (MQ=255)
                                                                                                   gTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                                                   <  1:1902888/150‑1 (MQ=255)
                                                                                                   gTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                                                   >  1:1816199/1‑150 (MQ=255)
                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                 <  2:1384322/150‑1 (MQ=255)
                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                 >  1:1079138/1‑150 (MQ=255)
                                                                                                       tttttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGATGTCGGTTTTTTTTATCAGTAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCt                                               >  1:254545/1‑150 (MQ=255)
                                                                                                         tttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTaa                                             >  2:775014/1‑150 (MQ=255)
                                                                                                            atttattgCTGAGTTTTGAATACATTTAAAACACCCACCTGAAACGCTTGCTGTTTTTTGTATCAGTAAGATGTTCAGAGTAATATTTAGACATAGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                                          <  1:329014/150‑1 (MQ=255)
                                                                                                            attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                                          >  1:1375028/1‑150 (MQ=255)
                                                                                                            attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAGCGTTATTCCATTCGAAACATATATTCATGATATATATATAAATATTTTCCTAATTg                                          >  2:1638178/1‑150 (MQ=255)
                                                                                                             ttgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGt                                         <  2:651633/150‑1 (MQ=255)
                                                                                                               tattgCTTAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                                       <  2:378292/149‑1 (MQ=255)
                                                                                                               tattgCTGAGTTTTTAATACATAGAAAACCGCCATCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGAGTAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTCATTGTTc                                       <  1:294323/149‑1 (MQ=255)
                                                                                                                tttgCTGAGTTTTGATTACATTGAAAACCGCAAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGATTATTTAGACATCGTTATACCACTCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                      <  2:1692013/149‑1 (MQ=255)
                                                                                                                attgCTGAGTTTTGAATACATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                      <  2:1559753/150‑1 (MQ=255)
                                                                                                                attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAACAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                      >  1:1489528/1‑150 (MQ=255)
                                                                                                                     tGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTTTtattatat                                 >  2:1748304/1‑148 (MQ=255)
                                                                                                                         ttttTAATACATTTAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACa                             <  1:840001/150‑1 (MQ=255)
                                                                                                                          tttGAATACATTGAAAAACGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                            <  1:338024/150‑1 (MQ=255)
                                                                                                                             taaTTCATTGAAAACAGCCATCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGGTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATATTCTTGAATTATATATATATATTTTCCTAATTGTTCTTATCTGACAGata                         <  1:1322578/149‑1 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                       <  1:1451528/150‑1 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                       <  1:786829/150‑1 (MQ=255)
                                                                                                                                 aCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtctc                     <  1:1529559/150‑1 (MQ=255)
                                                                                                                                  tacaaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATTTAAATATTTTACTAATTGTTCTTATCTGACAGATATGTCa                    >  2:1618209/6‑150 (MQ=255)
                                                                                                                                    caaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACt                  >  2:1499499/4‑150 (MQ=255)
                                                                                                                                     tGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACtt                 >  1:1030024/1‑150 (MQ=255)
                                                                                                                                      gAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa                >  2:254229/1‑150 (MQ=255)
                                                                                                                                        aaaCCTACAGCGGAAACGATGGCGGTTTTTTGTATCAGGAATATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg              <  1:680410/150‑1 (MQ=255)
                                                                                                                                        aaaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg              <  1:538380/150‑1 (MQ=255)
                                                                                                                                         aaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgg             >  2:1842417/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt           >  1:265096/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAggat           >  2:1259244/1‑148 (MQ=255)
                                                                                                                                                 gCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta     <  2:668533/150‑1 (MQ=255)
                                                                                                                                                   ggAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTtata   >  1:517636/1‑150 (MQ=255)
                                                                                                                                                    gAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAGATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa  <  2:1618791/150‑1 (MQ=255)
                                                                                                                                                    gAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa  >  1:82405/1‑150 (MQ=255)
                                                                                                                                                    gAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATAAATTCATGAAATATATATAAATATTTTCCTAATAGTTCTTATCTGACAGATAACTCACATAAGGCTTAATTATaa  >  1:1723276/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAA  >  NC_000913/729228‑729525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: