Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,377 A→T 6.3% intergenic (+37/‑206) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3770AT 6.3% 395.6 / 12.0 140intergenic (+37/‑206)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (71/60);  new base T (4/4);  total (75/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

Only 100 of 146 total aligned reads displayed.

TCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAA  >  NC_000913/729228‑729526
                                                                                                                                                     |                                                                                                                                                      
tCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGa                                                                                                                                                        <  1:276925/150‑1 (MQ=255)
  tGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGaaa                                                                                                                                                      >  1:1785370/1‑150 (MQ=255)
   ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                     >  2:103093/1‑150 (MQ=255)
        gACCGCGCCCGCTTTTACTGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGCGTGGAGGGATCTGGCCGAAAAGGCAACTGGAGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                <  1:1315943/150‑1 (MQ=255)
        gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                >  2:1833172/1‑150 (MQ=255)
        gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                <  1:1520986/150‑1 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                              >  2:981706/1‑150 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                              <  1:698252/150‑1 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTAGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                              <  1:400774/150‑1 (MQ=255)
           cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCgg                                                                                                                                             <  2:1033331/150‑1 (MQ=255)
           cgcgCCCGCTGTTACAGCTACCTGCATCGCGTCAGGGCGAAAACCAGCGATAATCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTGTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  1:302551/150‑1 (MQ=255)
            gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGt                                                                                                                                            <  2:444109/150‑1 (MQ=255)
                ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                        >  2:7036/1‑150 (MQ=255)
                ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                        >  2:1114915/1‑150 (MQ=255)
                 cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                                                       <  2:1781020/150‑1 (MQ=255)
                  gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                                                      <  2:656145/150‑1 (MQ=255)
                       tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCa                                                                                                                                 >  1:299620/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCTTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                                               <  2:808949/150‑1 (MQ=255)
                           gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGaa                                                                                                                             >  1:581496/1‑150 (MQ=255)
                             tACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGa                                                                                                                           >  2:493262/1‑150 (MQ=255)
                               ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGGTGGCGGTTTTTTGTATCAGGAAGAtt                                                                                                                         >  1:1791338/1‑149 (MQ=255)
                                cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                                                        >  2:1117966/1‑150 (MQ=255)
                                cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                                                        <  1:794740/150‑1 (MQ=255)
                                 tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                                                       >  2:399682/1‑150 (MQ=255)
                                  gCATCGCGTCTGGTCGAAATCCATCGATAAACCGGTGTGGATGGATCACGCCGAAAAGGCAACTGGTGATTTTTATTGATTTCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                      <  2:1332908/150‑1 (MQ=255)
                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                      <  2:943732/150‑1 (MQ=255)
                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                      <  1:1547714/150‑1 (MQ=255)
                                     tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAACCGCTGGCGGTTATTTGTATCAGGAAGATGTTCaga                                                                                                                   >  2:887652/1‑150 (MQ=255)
                                         gTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGaa                                                                                                               >  1:1490698/1‑150 (MQ=255)
                                               tCGAAAACCAGCGATTAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCTCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         <  2:1807459/150‑1 (MQ=255)
                                               tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         >  1:470624/1‑150 (MQ=255)
                                                cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                        <  1:1101264/150‑1 (MQ=255)
                                                    aaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                                                    >  2:1364353/1‑150 (MQ=255)
                                                      ccAGCGATAAACCTGTGTGGATGGATCAGGCCGAAAAGGCAACTGGCGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                                                  <  1:1144379/150‑1 (MQ=255)
                                                      ccAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                                                  >  2:1553078/1‑150 (MQ=255)
                                                        aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                                >  1:1533781/1‑150 (MQ=255)
                                                         gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAACCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                               >  1:57749/1‑150 (MQ=255)
                                                         gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                               <  2:543562/150‑1 (MQ=255)
                                                          cGATAAACCTGGGTGGTTGGATCTGGCCGAAAATGCTACTTGTGATTTTTATTGATTGCTGTGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtat                                                                                              <  1:201483/150‑1 (MQ=255)
                                                            aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGATGAATATTTAGACATCGTtataa                                                                                            >  2:724671/1‑149 (MQ=255)
                                                            aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAc                                                                                            >  1:162199/1‑150 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           <  1:1127723/150‑1 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  2:1175647/1‑150 (MQ=255)
                                                             tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  1:10083/1‑150 (MQ=255)
                                                              aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGTCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCa                                                                                          >  2:1208841/1‑150 (MQ=255)
                                                               aaCCGGTGTGGATGGATCAGGCCGAACAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                                                         <  1:1362094/150‑1 (MQ=255)
                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                     <  2:1627091/150‑1 (MQ=255)
                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                     >  1:1009646/1‑150 (MQ=255)
                                                                    gtgtGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaa                                                                                    <  1:536847/150‑1 (MQ=255)
                                                                           tggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                                             >  2:434986/1‑150 (MQ=255)
                                                                            ggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                                           >  2:1601645/1‑150 (MQ=255)
                                                                              atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTc                                                                         >  2:1815032/1‑150 (MQ=255)
                                                                               tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                                        >  2:436681/1‑150 (MQ=255)
                                                                                  ggCGGAAATGGCATCTGGTGAGTTTTCTTGAGTGCTGAGTTTTGAGGACGTTGAAAACCGCCAGCGGAACGGCTGGCGGTTTTTTGTATCAGGAAGATGTTGAGAGGTATATTTAGACATCGTTATAGCATTCGAATCATATATTCATGa                                                                     >  2:288624/1‑150 (MQ=255)
                                                                                  ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGa                                                                     <  2:1618648/150‑1 (MQ=255)
                                                                                     cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGGTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAat                                                                  >  2:851929/1‑150 (MQ=255)
                                                                                      gAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAata                                                                 >  2:1496123/1‑150 (MQ=255)
                                                                                        aaaGGCAACTGGTGATTTTTATTGATAGCGGAGTATTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAACATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATACATCAAaaata                                                               >  1:1270442/1‑150 (MQ=255)
                                                                                          aGGCAACTTGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAGACGCTGGCGGTTTTGTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCATTCGAAACATATATTCATGAAatatata                                                             >  2:66760/1‑150 (MQ=255)
                                                                                          aGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatata                                                             <  1:1320216/150‑1 (MQ=255)
                                                                                             cAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaa                                                          >  1:523401/1‑150 (MQ=255)
                                                                                                  ggTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAATATTTTCATAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtt                                                     >  1:1224048/1‑150 (MQ=255)
                                                                                                   gTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                                                    <  1:1902888/150‑1 (MQ=255)
                                                                                                   gTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                                                    >  1:1816199/1‑150 (MQ=255)
                                                                                                    tGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAATATGTTCAGAGGAATATTTAGACATCGTTATACCAATAGAAACATATATTCATGAAATATATATAAATAtttt                                                   >  2:1459468/1‑150 (MQ=255)
                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                  <  2:1384322/150‑1 (MQ=255)
                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                  >  1:1079138/1‑150 (MQ=255)
                                                                                                         tttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTaa                                              >  2:775014/1‑150 (MQ=255)
                                                                                                            atttattgCTGAGTTTTGAATACATTTAAAACACCCACCTGAAACGCTTGCTGTTTTTTGTATCAGTAAGATGTTCAGAGTAATATTTAGACATAGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                                           <  1:329014/150‑1 (MQ=255)
                                                                                                            attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTg                                           >  1:1375028/1‑150 (MQ=255)
                                                                                                            attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAGCGTTATTCCATTCGAAACATATATTCATGATATATATATAAATATTTTCCTAATTg                                           >  2:1638178/1‑150 (MQ=255)
                                                                                                               tattgCTTAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                                        <  2:378292/149‑1 (MQ=255)
                                                                                                                tttgCTGAGTTTTGATTACATTGAAAACCGCAAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGATTATTTAGACATCGTTATACCACTCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       <  2:1692013/149‑1 (MQ=255)
                                                                                                                attgCTGAGTTTTGAATACATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       <  2:1559753/150‑1 (MQ=255)
                                                                                                                attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       >  1:635790/1‑150 (MQ=255)
                                                                                                                  tgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTa                                     <  1:204155/150‑1 (MQ=255)
                                                                                                                   gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAt                                    >  2:44409/1‑150 (MQ=255)
                                                                                                                     tGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTTTtattatat                                  >  2:1748304/1‑148 (MQ=255)
                                                                                                                         ttttTAATACATTTAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACa                              <  1:840001/150‑1 (MQ=255)
                                                                                                                          tttGAATACATTGAAAAACGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                             <  1:338024/150‑1 (MQ=255)
                                                                                                                             gAATACATTGAAAACCGCCAGCGGAAACGCTTGCGGTTTTTTGTATAAGGAAGATGTTCACAGGAATTTTTATACATCGTTATAAAAATAGAAACATATATTAATGTAAAATATTTAAATATTTTAATTATTGTTCTTATCTGAAAGatt                          >  1:1623516/1‑149 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                        <  1:1451528/150‑1 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                        <  1:786829/150‑1 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGatata                        >  1:873390/1‑149 (MQ=255)
                                                                                                                                 aCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtctc                      <  1:455295/150‑1 (MQ=255)
                                                                                                                                   aTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCAc                    <  1:1821165/150‑1 (MQ=255)
                                                                                                                                    caaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACt                   >  2:1499499/4‑150 (MQ=255)
                                                                                                                                     tGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACtt                  >  1:1030024/1‑150 (MQ=255)
                                                                                                                                      gAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa                 >  2:254229/1‑150 (MQ=255)
                                                                                                                                      gAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa                 >  1:723080/1‑150 (MQ=255)
                                                                                                                                        aaaCCTACAGCGGAAACGATGGCGGTTTTTTGTATCAGGAATATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg               <  1:680410/150‑1 (MQ=255)
                                                                                                                                        aaaCCGCCAGCGGAAACGCTGGCGGTTTTTGTATCAGGAAGATGTTCAGAGAAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgg              >  2:1273640/1‑150 (MQ=255)
                                                                                                                                         aaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgg              >  2:1842417/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt            >  1:265096/1‑150 (MQ=255)
                                                                                                                                                 gCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta      <  2:581496/150‑1 (MQ=255)
                                                                                                                                                   ggAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTtata    >  1:517636/1‑150 (MQ=255)
                                                                                                                                                    gAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAGATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa   <  2:1618791/150‑1 (MQ=255)
                                                                                                                                                     aaaCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaaa  <  1:970399/150‑1 (MQ=255)
                                                                                                                                                     aaaCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa   <  2:1533065/150‑1 (MQ=255)
                                                                                                                                                     aaaCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaaa  <  1:376752/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAA  >  NC_000913/729228‑729526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: