Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,378 A→T 6.7% intergenic (+38/‑205) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3780AT 6.7% 390.5 / 14.0 140intergenic (+38/‑205)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (70/60);  new base T (4/5);  total (75/65)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 145 total aligned reads displayed.

TGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAA  >  NC_000913/729230‑729526
                                                                                                                                                    |                                                                                                                                                     
tGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGaaa                                                                                                                                                      >  1:1785370/1‑150 (MQ=255)
 ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                     >  2:103093/1‑150 (MQ=255)
      gACCGCGCCCGCTTTTACTGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGCGTGGAGGGATCTGGCCGAAAAGGCAACTGGAGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                <  1:1315943/150‑1 (MQ=255)
      gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                >  2:1833172/1‑150 (MQ=255)
      gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                <  1:1520986/150‑1 (MQ=255)
        ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                              >  2:981706/1‑150 (MQ=255)
        ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                              <  1:698252/150‑1 (MQ=255)
         cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  2:1091198/150‑1 (MQ=255)
         cgcgCCCGCTGTTACAGCTACCTGCATCGCGTCAGGGCGAAAACCAGCGATAATCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTGTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  1:302551/150‑1 (MQ=255)
          gctcccTCTTTTACAGCTACCTGCATCGCGGCTGGTCCAAAACCATCGATAAAGCGGTGTGGATGGATCACGCCGAAAAGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTGACTACATTGAAAACCGCCAGCGTGTCCGCTGGCGGt                                                                                                                                            <  2:117426/147‑1 (MQ=255)
          gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGt                                                                                                                                            <  2:444109/150‑1 (MQ=255)
           cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACAAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                           <  2:1451538/150‑1 (MQ=255)
              ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                        >  2:7036/1‑150 (MQ=255)
              ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                        >  2:1114915/1‑150 (MQ=255)
               cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                                                       <  2:1781020/150‑1 (MQ=255)
                gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                                                      <  2:656145/150‑1 (MQ=255)
                       cAGCTACCTGCATCGCTTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                                               <  2:808949/150‑1 (MQ=255)
                         gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGaa                                                                                                                             >  1:581496/1‑150 (MQ=255)
                          cTACCTGCATCGCGTCTGGTCGAAAAGCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                                            <  2:15069/150‑1 (MQ=255)
                           tACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGa                                                                                                                           >  2:493262/1‑150 (MQ=255)
                             ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGGTGGCGGTTTTTTGTATCAGGAAGAtt                                                                                                                         >  1:1791338/1‑149 (MQ=255)
                             ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCAGGAAGAtt                                                                                                                         >  1:1798498/1‑149 (MQ=255)
                               tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                                                       >  2:399682/1‑150 (MQ=255)
                                gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                      <  1:1547714/150‑1 (MQ=255)
                                gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                      <  2:943732/150‑1 (MQ=255)
                                   tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAACCGCTGGCGGTTATTTGTATCAGGAAGATGTTCaga                                                                                                                   >  2:887652/1‑150 (MQ=255)
                                       gTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGaa                                                                                                               >  1:1490698/1‑150 (MQ=255)
                                             tcgattaCCTGCGTTAAACCTGTGTGGATGTATCACGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTTAAAACCTCCAGCTGAAACGCTGTCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         <  1:368866/144‑1 (MQ=255)
                                             tCGAAAACCAGCGATTAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCTCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         <  2:1807459/150‑1 (MQ=255)
                                              cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                        <  1:1101264/150‑1 (MQ=255)
                                                aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAc                                                                                                      >  2:1821165/1‑150 (MQ=255)
                                                  aaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAt                                                                                                    >  2:1364353/1‑150 (MQ=255)
                                                    ccAGCGATAAACCTGTGTGGATGGATCAGGCCGAAAAGGCAACTGGCGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                                                  <  1:1144379/150‑1 (MQ=255)
                                                     cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                                 >  1:777774/1‑150 (MQ=255)
                                                      aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                                >  1:1533781/1‑150 (MQ=255)
                                                       gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                               <  2:543562/150‑1 (MQ=255)
                                                        cGATAAACCTGGGTGGTTGGATCTGGCCGAAAATGCTACTTGTGATTTTTATTGATTGCTGTGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtat                                                                                              <  1:201483/150‑1 (MQ=255)
                                                         gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGTAAAATGTTCAGAGGAAAATTTAGACATCGTtata                                                                                             >  1:318987/1‑150 (MQ=255)
                                                          aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGATGAATATTTAGACATCGTtataa                                                                                            >  2:724671/1‑149 (MQ=255)
                                                          aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAc                                                                                            >  1:162199/1‑150 (MQ=255)
                                                           tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           <  1:1127723/150‑1 (MQ=255)
                                                           tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  1:10083/1‑150 (MQ=255)
                                                           tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  2:1175647/1‑150 (MQ=255)
                                                           tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           <  2:1346502/150‑1 (MQ=255)
                                                           tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  1:413381/1‑150 (MQ=255)
                                                           tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  1:490926/1‑150 (MQ=255)
                                                                 ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                     <  2:1627091/150‑1 (MQ=255)
                                                                 ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                     >  1:1009646/1‑150 (MQ=255)
                                                                 ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                     >  1:605317/1‑150 (MQ=255)
                                                                  gtgtGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaa                                                                                    <  1:536847/150‑1 (MQ=255)
                                                                         tggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                                             >  2:434986/1‑150 (MQ=255)
                                                                            atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑tta                                                                         >  1:890177/1‑149 (MQ=255)
                                                                             tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCa                                                                        >  2:436681/1‑150 (MQ=255)
                                                                               attccGAATAGGCAAATTGTGTTTTTTTATGATTTTTGAGTTTTTAATAAATTGTTAACCGCCAGATGAATAGCTGGCTGTTTTTTGAATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAATCTTATA‑TTCATg                                                                      <  1:69779/147‑1 (MQ=255)
                                                                                ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGa                                                                     <  2:1618648/150‑1 (MQ=255)
                                                                                  cctaaaaGTCAACTTGTGATTTTTATTGATTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGaaa                                                                   <  1:184142/147‑1 (MQ=255)
                                                                                  ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGaaa                                                                   <  2:1555121/150‑1 (MQ=255)
                                                                                   cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGGTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAat                                                                  >  2:851929/1‑150 (MQ=255)
                                                                                    gAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAata                                                                 >  2:1496123/1‑150 (MQ=255)
                                                                                      aaaGGCAACTGGTGATTTTTATTGATAGCGGAGTATTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAACATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TACATCAAaaata                                                               >  1:1270442/1‑150 (MQ=255)
                                                                                        aGGCAACTTGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAGACGCTGGCGGTTTTGTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCATTCGAAACATATA‑TTCATGAAatatata                                                             >  2:66760/1‑150 (MQ=255)
                                                                                        aGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAatatata                                                             <  1:1320216/150‑1 (MQ=255)
                                                                                                ggTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAATATTTTCATAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATAtt                                                     >  1:1224048/1‑150 (MQ=255)
                                                                                                  tGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTTTTGTATCAGGAATATGTTCAGAGGAATATTTAGACATCGTTATACCAATAGAAACATATA‑TTCATGAAATATATATAAATAtttt                                                   >  2:1459468/1‑150 (MQ=255)
                                                                                                   gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTc                                                  >  1:1079138/1‑150 (MQ=255)
                                                                                                       tttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTaa                                              >  2:775014/1‑150 (MQ=255)
                                                                                                          atttattgCTGAGTTTTGAATACATTTAAAACACCCACCTGAAACGCTTGCTGTTTTTTGTATCAGTAAGATGTTCAGAGTAATATTTAGACATAGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTg                                           <  1:329014/150‑1 (MQ=255)
                                                                                                          attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTg                                           >  1:1375028/1‑150 (MQ=255)
                                                                                                          attgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACAGCGTTATTCCATTCGAAACATATA‑TTCATGATATATATATAAATATTTTCCTAATTg                                           >  2:1638178/1‑150 (MQ=255)
                                                                                                           ttgattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGt                                          <  2:651633/150‑1 (MQ=255)
                                                                                                             tattgCTTAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTc                                        <  2:378292/149‑1 (MQ=255)
                                                                                                             tattgCTGAGTTTTTAATACATAGAAAACCGCCATCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATTTTCAGAGTAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTCATTGTTc                                        <  1:294323/149‑1 (MQ=255)
                                                                                                              tttgCTGAGTTTTGATTACATTGAAAACCGCAAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGATTATTTAGACATCGTTATACCACTCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       <  2:1692013/149‑1 (MQ=255)
                                                                                                              attgCTGAGTTTTGAATACATTGAAACCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       <  2:1559753/150‑1 (MQ=255)
                                                                                                              attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       >  1:635790/1‑150 (MQ=255)
                                                                                                              attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       <  2:1886723/150‑1 (MQ=255)
                                                                                                                tgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTa                                     <  1:204155/150‑1 (MQ=255)
                                                                                                                 gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAt                                    >  2:44409/1‑150 (MQ=255)
                                                                                                                 gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAt                                    >  2:376752/1‑150 (MQ=255)
                                                                                                                   tGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTTTtattatat                                  >  2:1748304/1‑148 (MQ=255)
                                                                                                                       ttttTAATACATTTAAAACCGCCAGCGTAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACa                              <  1:840001/150‑1 (MQ=255)
                                                                                                                           taaTTCATTGAAAACAGCCATCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGGTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCTTGAATTATATATATATATTTTCCTAATTGTTCTTATCTGACAGata                          <  1:1322578/149‑1 (MQ=255)
                                                                                                                           gAATACATTGAAAACCGCCAGCGGAAACGCTTGCGGTTTTTTGTATAAGGAAGATGTTCACAGGAATTTTTATACATCGTTATAAAAATAGAAACATATA‑TTAATGTAAAATATTTAAATATTTTAATTATTGTTCTTATCTGAAAGatt                          >  1:1623516/1‑149 (MQ=255)
                                                                                                                             aTACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                        <  1:1451528/150‑1 (MQ=255)
                                                                                                                             aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                        <  1:786829/150‑1 (MQ=255)
                                                                                                                               aCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtctc                      <  1:455295/150‑1 (MQ=255)
                                                                                                                                tacaaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATTTAAATATTTTACTAATTGTTCTTATCTGACAGATATGTCa                     >  2:1618209/6‑150 (MQ=255)
                                                                                                                                 aTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCAc                    <  1:1821165/150‑1 (MQ=255)
                                                                                                                                   tGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACtt                  >  1:1030024/1‑150 (MQ=255)
                                                                                                                                    gAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa                 >  2:254229/1‑150 (MQ=255)
                                                                                                                                      aaaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg               <  1:538380/150‑1 (MQ=255)
                                                                                                                                      aaaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg               <  2:1624519/150‑1 (MQ=255)
                                                                                                                                      aaaCCGCCAGCGGAAACGCTGGCGG‑TTTTTGTATCAGGAAGATGTTCAGAGAAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgg              >  2:1273640/1‑150 (MQ=255)
                                                                                                                                         ccGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt            >  1:265096/1‑150 (MQ=255)
                                                                                                                                         ccGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAggat            >  2:1259244/1‑148 (MQ=255)
                                                                                                                                                 ggAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTtata    >  1:517636/1‑150 (MQ=255)
                                                                                                                                                  gAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa   >  1:82405/1‑150 (MQ=255)
                                                                                                                                                   aaaCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaaa  <  1:970399/150‑1 (MQ=255)
                                                                                                                                                   aaaCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa   <  2:1533065/150‑1 (MQ=255)
                                                                                                                                                   aaaCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaaa  <  1:376752/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATA‑TTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAA  >  NC_000913/729230‑729526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑