Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,331 G→T 15.1% G142V (GGT→GTT)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,3310GT15.1% 320.4 / 34.9 137G142V (GGT→GTT) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (58/57);  new base T (14/7);  total (72/65)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGG  >  NC_000913/756182‑756477
                                                                                                                                                     |                                                                                                                                                  
gCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCgg                                                                                                                                                    >  2:1105116/1‑150 (MQ=255)
gCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCgg                                                                                                                                                    >  2:1450476/1‑150 (MQ=255)
  cgcaaGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTc                                                                                                                                                  <  1:1428017/147‑1 (MQ=255)
  cGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTc                                                                                                                                                  <  2:476420/150‑1 (MQ=255)
     aaGCGATTCTGGAACTCGAACCCATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGGTACGGTCagc                                                                                                                                               <  1:682203/150‑3 (MQ=255)
     aaGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACg                                                                                                                                               <  2:1044556/150‑1 (MQ=255)
             cTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                                                       >  2:847861/1‑150 (MQ=255)
             cTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                                                       <  2:1793707/150‑1 (MQ=255)
             cTGGAACTCGAACACATGGGCCTGCCGTGCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                                                       <  1:1410425/150‑1 (MQ=255)
              tGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGCTGATGGTCGTATCTTTCAACGTCCGTTTGGCTTTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGc                                                                                                                                      <  1:915370/150‑1 (MQ=255)
              tGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGCCCGTACCGTTCACGCACTGTTGc                                                                                                                                      >  1:1474793/1‑150 (MQ=255)
              tGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGc                                                                                                                                      <  2:1406687/150‑1 (MQ=255)
               ggAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcc                                                                                                                                     >  1:1617963/1‑149 (MQ=255)
                  aCTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCacac                                                                                                                                  <  1:220918/150‑1 (MQ=255)
                     cgaccccaTGCGCCTGCCGTTCTCGCGTCTCGATGAGGGCCGTATCTATCAACGTCCGTTTGGCGGTCAGCCGCAAACCTTCGGCGGCGAGCAGGCGGCACGCAATGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCt                                                                                                                               <  2:300259/144‑1 (MQ=255)
                     cGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCt                                                                                                                               <  1:682874/150‑1 (MQ=255)
                     cGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCt                                                                                                                               <  1:1489382/150‑1 (MQ=255)
                     cGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCt                                                                                                                               >  2:610383/1‑150 (MQ=255)
                      gAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCtt                                                                                                                              >  2:716075/1‑150 (MQ=255)
                          ccctGGGCCTGCCGTTCTCGCGTCTCGATCATGGTCGTATCTATCAACGTCCGTATGGCGTTCAGTGGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATc                                                                                                                          <  2:630758/147‑1 (MQ=255)
                          acaTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATc                                                                                                                          >  1:580993/1‑150 (MQ=255)
                           caTGGGCCTGCCGTTCTCGCGTCTCGATGCTGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATca                                                                                                                         >  1:963473/1‑150 (MQ=255)
                           caTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCCGCAGCTGCCCGTACCGGTCACGCACTGTTGCCCCCGCTTTATca                                                                                                                         >  1:957137/1‑150 (MQ=255)
                           caTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATca                                                                                                                         >  2:1841574/1‑150 (MQ=255)
                            aggggCCTGCCGTTCTCGCGTCTCGATGATTGTCGAAGCTATCAACGTCCGTGTGGCGGTCAGTCGAAACACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATcag                                                                                                                        <  1:274103/148‑1 (MQ=255)
                             tGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAATCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagc                                                                                                                       <  1:1612848/150‑1 (MQ=255)
                                gCCTGCCGTTATCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGa                                                                                                                    >  1:389741/1‑150 (MQ=255)
                                   tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAcc                                                                                                                 <  1:1054091/150‑1 (MQ=255)
                                   tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGACAGACGAAAAACTTCGGCGGCGCGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGACACGCACTCTAGCACACGCTTTATCAGCAGAAcc                                                                                                                 >  1:322685/1‑150 (MQ=255)
                                     ccGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTg                                                                                                               <  2:464710/150‑1 (MQ=255)
                                     ccGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTg                                                                                                               <  1:805315/150‑1 (MQ=255)
                                          ctcGCGTCTCGATGTTGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGCCCGTACCGTTCCCGCACTGTTGCCCACGCTTTATCAGCAGAACCTGaaaac                                                                                                          >  2:1007919/1‑149 (MQ=255)
                                            cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacc                                                                                                        <  1:1850219/150‑1 (MQ=255)
                                            cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacc                                                                                                        >  1:1235784/1‑150 (MQ=255)
                                               gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccaca                                                                                                     <  2:968672/150‑1 (MQ=255)
                                                gctcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacac                                                                                                    <  2:1229292/149‑1 (MQ=255)
                                                 ctcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacacc                                                                                                   >  2:1415027/1‑150 (MQ=255)
                                                  tcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacacca                                                                                                  <  1:1009602/150‑1 (MQ=255)
                                                   cGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCCGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGCACCTGAAAAaccacaccac                                                                                                 >  2:1754827/1‑150 (MQ=255)
                                                   cGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacaccac                                                                                                 >  1:609417/1‑150 (MQ=255)
                                                   cGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacaccac                                                                                                 >  1:544609/1‑150 (MQ=255)
                                                     atgatgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACAccacca                                                                                               >  1:539673/1‑150 (MQ=255)
                                                      tgatgGTCGTAGCTCTCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAt                                                                                              <  2:449875/150‑1 (MQ=255)
                                                      tgaggGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAt                                                                                              <  2:1115296/150‑1 (MQ=255)
                                                         tgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAtttt                                                                                           >  1:1296047/1‑150 (MQ=255)
                                                          ggTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGGAAAACCACACCACCATTTtc                                                                                          <  2:1585539/150‑1 (MQ=255)
                                                            tCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTtctc                                                                                        <  1:1855888/150‑1 (MQ=255)
                                                              gTATCTATCAACGTCCGTTTTGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCTGCAGCAGCTGACCTTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCg                                                                                      <  2:1319934/150‑1 (MQ=255)
                                                                  ctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGaaa                                                                                                            <  1:726175/124‑1 (MQ=255)
                                                                   tatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACTCGCTTTATCAGCTGAACGTGAAAACCCACACCACCATTTTCTCCGAGTgg                                                                                 >  2:333106/1‑150 (MQ=255)
                                                                   tatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTgg                                                                                 <  1:812026/150‑1 (MQ=255)
                                                                   tatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTgg                                                                                 >  1:1701098/1‑150 (MQ=255)
                                                                      cAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTAt                                                                              >  2:644721/1‑150 (MQ=255)
                                                                          gTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATgcgc                                                                          <  2:550629/150‑1 (MQ=255)
                                                                             cGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGCACCTGAAAAACCACACCACCATATTCGCCGAGTTGTAGGCGCTgg                                                                       >  2:126035/1‑150 (MQ=255)
                                                                             cGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTgg                                                                       <  2:1580876/150‑1 (MQ=255)
                                                                                 tGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCt                                                                   >  1:17368/1‑150 (MQ=255)
                                                                                  ggCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGACCCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTg                                                                  >  2:590996/1‑150 (MQ=255)
                                                                                  ggCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTg                                                                  >  2:572126/1‑150 (MQ=255)
                                                                                    cGGTCAGTCGAAAATCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGt                                                                >  2:1047593/1‑150 (MQ=255)
                                                                                    cGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGt                                                                <  2:1857056/150‑1 (MQ=255)
                                                                                      ggcAGTCGAAAACCTTCTGAGGTTAGCAGGCGGCACTGACTGGGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCTGCTGAACCTGAAAAACCACACCTCCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGa                                                              <  2:89843/148‑1 (MQ=255)
                                                                                        cAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaa                                                            >  2:571206/1‑150 (MQ=255)
                                                                                         aGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaaa                                                           >  2:753253/1‑150 (MQ=255)
                                                                                           tCGAAAATATTCGGCGGCGTGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGAACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAAc                                                         <  2:1671548/150‑1 (MQ=255)
                                                                                           tCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACCCCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAAc                                                         >  2:1670866/1‑150 (MQ=255)
                                                                                             gAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGAAGCAGACCGTACCTGTCACGAACTTATGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAGTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCa                                                       >  2:776890/1‑150 (MQ=255)
                                                                                             gAAAAACTTCGGCGGCGAGCAGGCGGAACGCACTGCGGCAGCAGCTGACAGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACAATTTTCTCCGAGTGGTATGCGCTGGATCTGGTCAAAAACCa                                                       >  1:1786890/1‑150 (MQ=255)
                                                                                              aaaaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCCCCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg                                                      >  2:1009368/1‑150 (MQ=255)
                                                                                              aaaaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg                                                      <  2:1909164/150‑1 (MQ=255)
                                                                                               aaaaCTTCGGCGGCGCGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCCCGCCCTGTTGCCCACGCTTTATCAGCAGAACCTGAAACACCACACCACCCTTTTCTCCGAGTGGTATGCGCTGGAGCTGGTGACAAACCCgg                                                     >  1:1005584/1‑150 (MQ=255)
                                                                                               aaaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAgg                                                     <  1:730544/150‑1 (MQ=255)
                                                                                                aaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGa                                                    >  2:321927/1‑150 (MQ=255)
                                                                                                   cTTCGGCGGCGAGCAGGCGGAACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATAGGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATgg                                                 >  1:1047270/1‑150 (MQ=255)
                                                                                                    atccgcggcgAGCAGGCGGCACGCAGTGCGGCAGCAGCTGACCGTACCGGGCACGCACAGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGgc                                                <  1:360257/146‑1 (MQ=255)
                                                                                                         cggcgAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCTGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggt                                           <  2:553848/150‑1 (MQ=255)
                                                                                                            cgAGCAGGCGGCACGCACTGAGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGACCCTGAAAAACCACACCACCAATTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtggt                                        >  2:830677/1‑150 (MQ=255)
                                                                                                               ccAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTggg                                     <  1:1885063/149‑1 (MQ=255)
                                                                                                                cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                    <  1:1841574/150‑1 (MQ=255)
                                                                                                                 aGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGtt                                   <  2:1541797/150‑1 (MQ=255)
                                                                                                                 aGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGtt                                   >  1:521207/1‑150 (MQ=255)
                                                                                                                  ggcgtcACGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg                                  <  1:1135268/150‑1 (MQ=255)
                                                                                                                  ggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg                                  >  2:454382/1‑150 (MQ=255)
                                                                                                                  ggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCACTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg                                  >  2:152859/1‑150 (MQ=255)
                                                                                                                   gcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGt                                 >  1:72435/1‑150 (MQ=255)
                                                                                                                           cacTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTGCCGCACt                         >  2:994821/1‑150 (MQ=255)
                                                                                                                              tGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTAGCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGTGTGGTATGCGCTGGATCTGGTGAAACACCAGGATGGCGCGGTGGTGGGTTGTACCGCACtgtg                      <  1:70187/150‑1 (MQ=255)
                                                                                                                              ggCGGCAGCAGCGGACCGCACGCGTCACGCACTGTGCCACACGCTTTAACCCCACAACCTCAAAACCCCCCCCCCCATTTTCTCCGAGTGGTATGCGCTGCTTCTGGTTAAAACCCTGGTGGGGGCGGGGGGGGGTTGTACCGCACtgtg                      <  2:963473/149‑1 (MQ=255)
                                                                                                                                cGGCAGCAGCTGCCCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGGGGTATGCGCTGGATCTGGTGAAAAACCAGGCTGGCGCGGTGGTGGGTTGTACCGCACTGTGCa                    >  1:1298242/1‑150 (MQ=255)
                                                                                                                                 ggCAGCAGCTGACCGTACCGGGCACGCACTGTTGCACACGCTTTATCCGCAGACCCTGAAAACCCACACCCCCATGTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCAt                   <  1:1291393/150‑1 (MQ=255)
                                                                                                                                    agcagcTGACCGTACCGTTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGAGGGTTGTCCCGCCCTTGTCATCGa                >  2:689190/1‑150 (MQ=255)
                                                                                                                                      cagcTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGaca              >  2:915348/1‑148 (MQ=255)
                                                                                                                                        gcTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAAcc            <  2:761110/150‑1 (MQ=255)
                                                                                                                                          tGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCgg          <  2:1617963/150‑1 (MQ=255)
                                                                                                                                           gACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt         >  2:1039925/1‑150 (MQ=255)
                                                                                                                                             ccGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGa       >  1:1218141/1‑150 (MQ=255)
                                                                                                                                             ccGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGAATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGa       <  2:710725/150‑1 (MQ=255)
                                                                                                                                                tACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGt    >  1:1063501/1‑150 (MQ=255)
                                                                                                                                                tACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGt    >  1:6242/1‑150 (MQ=255)
                                                                                                                                                  ccGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTgg  >  1:1500225/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGG  >  NC_000913/756182‑756477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑