Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,576 T→C 7.6% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5760TC 7.6% 330.1 / 12.9 120intergenic (+21/+220)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base T (70/39);  new base C (6/3);  total (76/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

Only 100 of 121 total aligned reads displayed.

ATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAA  >  NC_000913/857427‑857721
                                                                                                                                                     |                                                                                                                                                 
aTCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAt                                                                                                                                                   <  1:988358/150‑1 (MQ=255)
  cTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTTATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATAc                                                                                                                                                 >  2:116409/1‑150 (MQ=255)
    ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCGGAACGCGTGATCGACTTTAGCGGTAATTACTAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGt                                                                                                                                              >  2:888387/1‑150 (MQ=255)
      gTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTc                                                                                                                                             >  1:1469172/1‑150 (MQ=255)
        ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGTAATGACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgg                                                                                                                                           >  1:993994/1‑150 (MQ=255)
        ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATTACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgg                                                                                                                                           >  1:1702021/1‑150 (MQ=255)
        ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgg                                                                                                                                           >  2:626221/1‑150 (MQ=255)
         tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGAGCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTCTCggg                                                                                                                                          >  1:265180/1‑150 (MQ=255)
          cacaCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                         >  1:189749/1‑150 (MQ=255)
          cacaCGACCGTGACTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACGTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                         <  2:562715/150‑1 (MQ=255)
           acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                        >  2:1217749/1‑150 (MQ=255)
           acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                        <  2:1878986/150‑1 (MQ=255)
            cacGACCGTGAGTTCGTAAGCTCCCTGGAGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGCCTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACACCGATACTGTCGGGGtt                                                                                                                                       >  1:98547/1‑149 (MQ=255)
             acGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGa                                                                                                                                      >  1:1184310/1‑150 (MQ=255)
               gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcg                                                                                                                                    <  1:1381417/150‑1 (MQ=255)
               gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcg                                                                                                                                    <  2:1775469/150‑1 (MQ=255)
                  cGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcg                                                                                                                                 <  2:938105/150‑1 (MQ=255)
                    tGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACggcggc                                                                                                                               >  1:1800144/1‑150 (MQ=255)
                     gAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCt                                                                                                                              <  2:579683/150‑1 (MQ=255)
                       gTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtga                                                                                                                            >  1:1827369/1‑150 (MQ=255)
                        ttCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgat                                                                                                                           <  2:23632/150‑1 (MQ=255)
                           gTAAGCTCCCTGGCGACCCGCATTCTGGATATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAc                                                                                                                        <  2:96896/150‑1 (MQ=255)
                            tAAGCTCCCTTGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACa                                                                                                                       <  1:1121720/150‑1 (MQ=255)
                            tAAGCTCCCGGCCCACCCGATTTCTGTAATACACCCCGCACCGGGTTTTCGACTTTAGGGTTAATTCCCAATATAACCTGCTTTTTAACGGTTTCGTTTAAGTTATTACCCGCCACCCCCAAACTGTCGGGGTGACGGCGGCTGATGACa                                                                                                                       <  2:606308/150‑1 (MQ=255)
                                  cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                 >  1:1106950/1‑150 (MQ=255)
                                  cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                 >  2:690690/1‑150 (MQ=255)
                                  cccTGGCGACCCGCATTCTGGAAAACACCCCGGAACGCGTGATCGACTTAAGCGGTAATTACGAATATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                 <  1:774988/150‑1 (MQ=255)
                                   ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaa                                                                                                                >  1:212646/1‑150 (MQ=255)
                                      ggCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACTAAGATTACCTGCGTAGTAAAGGGATCGAGAATGTTATTATCCGACACCCCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAAt                                                                                                             <  1:1896801/150‑1 (MQ=255)
                                           cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTTGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGAATAATAGCCt                                                                                                        >  2:80691/1‑150 (MQ=255)
                                               cATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATg                                                                                                    <  2:35492/150‑1 (MQ=255)
                                                     ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTAc                                                                                              >  1:1623917/1‑150 (MQ=255)
                                                        aaTCCCCCGGTAACGGGTTATCGACTTTAGGGTTAATTACCAATATTACTTGGTTTTTAATGGTATCGTTTATGTTTTTTTCGGTCACCCTTATACTGTGGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCt                                                                                           <  2:1696535/150‑1 (MQ=255)
                                                        aaTCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGAGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCt                                                                                           >  2:1671098/1‑150 (MQ=255)
                                                           cACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTAt                                                                                        >  1:341004/1‑150 (MQ=255)
                                                           cACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTAt                                                                                        >  1:552756/1‑150 (MQ=255)
                                                             ccccGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCTAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCa                                                                                      <  2:545684/150‑1 (MQ=255)
                                                              cccGGACCGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAg                                                                                     <  2:1774617/150‑1 (MQ=255)
                                                              cccGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAg                                                                                     >  1:448047/1‑150 (MQ=255)
                                                               ccGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATACCCTGATGCCCAACCATTATCAgg                                                                                    >  1:1159920/1‑150 (MQ=255)
                                                                            tCGACTTTAGCGGTATTTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTagc                                                                       <  1:1217749/150‑3 (MQ=255)
                                                                            tCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTAcc                                                                       <  2:1532710/150‑1 (MQ=255)
                                                                             cGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCt                                                                      >  2:1620776/1‑150 (MQ=255)
                                                                              gACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTg                                                                     >  1:586464/1‑150 (MQ=255)
                                                                                      cGGTAATTACAAAGATTACCAGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTAGGGGTAACGGCGGCTGATGACAAATTCAAAAAATCCTGATTCCATACGCTTAAAAGACCTACGCAGTACCAGAAaaatat                                                             >  2:1152485/1‑150 (MQ=255)
                                                                                             tACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCTTCACCCCGATAATGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCATGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCt                                                      >  2:14040/1‑150 (MQ=255)
                                                                                             tACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCt                                                      >  1:1325118/1‑150 (MQ=255)
                                                                                             tACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACACCGATACTGTCGGGGTGACGGCTGCTGATGACAAAAGAAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCt                                                      >  2:496301/1‑150 (MQ=255)
                                                                                               cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCAGCCCGTTACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                    <  2:311954/150‑1 (MQ=255)
                                                                                               cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAATTGCAAAATAGCCTGCTGCGCTACGCTTATCAGGCCTACGCCGTACCTGCAATATATTGAATCTGc                                                    >  1:126245/1‑150 (MQ=255)
                                                                                               cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                    <  2:1247080/150‑1 (MQ=255)
                                                                                               cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                    >  1:1067907/1‑150 (MQ=255)
                                                                                                gAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCa                                                   >  2:1051453/1‑150 (MQ=255)
                                                                                                 aaGATTACCTGCGTAGTAAAGGGATCGAGTATTTGATTATACGTCACCCCGATACTGTCGGTGTGACGTAGGCTGATGCGATATGCAAAATAGCATGATGCGCTAAGCTTATCAGGCCTACGAAGTACATGCAATTTATTGAATCTGCAg                                                  >  1:107097/1‑150 (MQ=255)
                                                                                                        ccTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTg                                           >  1:1663145/1‑150 (MQ=255)
                                                                                                        ccTGCGTAGAAAAGGGAGCTCGTAAGTTATAAGCCGTCCCCCCCATACTGGGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTTCCTGCAATATATTGAATCTGCAGGACTTTg                                           <  1:626221/150‑1 (MQ=25)
                                                                                                         cTGCGTAGTAAACGGATCGAGTAAGTGATTATACGTCACCCCCATACTGTCGGGGTGACGGCGGCTGATGACAAAAGCAAAATACCCTGATGAGATACGCTTATCAGGCCTACGCAGTAACTCCAATATATTTAATCTCCAGGACTTTga                                          >  2:619127/1‑149 (MQ=38)
                                                                                                          tGCGTAGTAAAGGTTGCGAGTATGTGATTATCCGTCACCCCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                         <  1:1186737/150‑1 (MQ=38)
                                                                                                          tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                         >  1:45422/1‑150 (MQ=255)
                                                                                                            cGTTGTAAAGGGATCGAGTAAGTGTTTATCCGTCACCCAGATTCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCGTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGATTCTGCAGGACTTTGTAgg                                       <  1:34195/150‑1 (MQ=38)
                                                                                                               agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                    >  2:772920/1‑150 (MQ=255)
                                                                                                               agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                    >  2:1103234/1‑150 (MQ=255)
                                                                                                                 taAAGGGATCTATTAAGTGATTATCCTTCACCCCTATACTGTCGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGa                                  <  2:189749/150‑1 (MQ=37)
                                                                                                                   aaGTGATCTAGAAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                <  1:911827/150‑1 (MQ=255)
                                                                                                                       gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAGAAGGc                            >  2:1327927/1‑150 (MQ=255)
                                                                                                                        aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                           >  1:875517/1‑150 (MQ=255)
                                                                                                                             gTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAGTTTGTAGGCCGGATAAGGCGTTAAc                      >  2:1610759/1‑150 (MQ=255)
                                                                                                                                aGTGATACTCCGACACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                   <  2:1843301/150‑1 (MQ=255)
                                                                                                                                 ttGAATATCCGTCACCCCGACACTGTCGGGGTGACGGCGGCTGAGGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATAGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                  <  2:107872/149‑1 (MQ=255)
                                                                                                                                 gTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                  <  1:690690/150‑1 (MQ=255)
                                                                                                                                  tGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                 <  2:1903513/150‑1 (MQ=255)
                                                                                                                                   gATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCCTGATGCCCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAGTTTGTAGGCCGGATAAGGCGTTAACCCCGCa                >  2:1749618/1‑150 (MQ=255)
                                                                                                                                   gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                >  2:77476/1‑150 (MQ=255)
                                                                                                                                    aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAata                                                                <  2:315364/101‑1 (MQ=255)
                                                                                                                                    aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTACAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAAGCCGCAt               >  1:1281750/1‑150 (MQ=255)
                                                                                                                                      tATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa             >  2:1518900/1‑150 (MQ=255)
                                                                                                                                      tATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa             >  2:618638/1‑150 (MQ=255)
                                                                                                                                      tATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa             >  2:1511221/1‑150 (MQ=255)
                                                                                                                                      tATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa             >  1:698423/1‑150 (MQ=255)
                                                                                                                                       aTCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAGAAGGCGTTAACGCCGCATCAg            >  1:292186/1‑150 (MQ=255)
                                                                                                                                       aTCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACATGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAAAAGGCGTTATCGCCtcatcat            >  2:1797652/1‑149 (MQ=255)
                                                                                                                                        tCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg           >  2:1248108/1‑150 (MQ=255)
                                                                                                                                        gccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg           >  1:112116/2‑150 (MQ=255)
                                                                                                                                         ccGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc          >  1:481320/1‑150 (MQ=255)
                                                                                                                                         ccGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc          <  1:1135333/150‑1 (MQ=255)
                                                                                                                                          cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa         >  2:925833/1‑150 (MQ=255)
                                                                                                                                          cGTCACCCCGATACTGTCGCGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCCCATCAGGCa         >  2:1810425/1‑150 (MQ=255)
                                                                                                                                           gTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt        >  2:1362313/1‑150 (MQ=255)
                                                                                                                                            tCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAtt       <  2:1469172/150‑1 (MQ=255)
                                                                                                                                            ccACCCCCATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAtt       <  1:1758916/149‑1 (MQ=255)
                                                                                                                                             cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa      >  2:397934/1‑150 (MQ=255)
                                                                                                                                             cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAAGCTGCAGGACTTTGTAGGCCGGCTAAGGCGTTAACGCCGCATCAGGCATTa      >  1:1202183/1‑150 (MQ=255)
                                                                                                                                              aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTaa     >  1:1792357/1‑150 (MQ=255)
                                                                                                                                              aCCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTaa     >  2:1648176/1‑150 (MQ=255)
                                                                                                                                              aCCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTGATCAAGACTACGCAGTACCTGCAATAAATTGAGGATGCAGGACAGTGTCGGCCGCAGACGGCGGTAACGCCGCAGCAggaagtca     >  2:295092/1‑144 (MQ=255)
                                                                                                                                               ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTATGCCGTATAAGGCGTTAACGCCGCATCAGGCATTAAc    >  2:965198/1‑150 (MQ=255)
                                                                                                                                               ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc    >  1:813899/1‑150 (MQ=255)
                                                                                                                                               ccccGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc    >  2:1109223/1‑150 (MQ=255)
                                                                                                                                                cccGATACTTTCGGTGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACa   <  2:993994/150‑1 (MQ=255)
                                                                                                                                                 ccGATACTGTCGGTGTGACGGCGGCTGATGACAAATGCAAAATAGCATGATGCGCTACGCTTATCAGACCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGCTAACGCCGCATCAGGCATTAACaa  <  1:116409/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAA  >  NC_000913/857427‑857721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: