Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 900,208 T→A 5.1% D123V (GAT→GTT) ‡ artJ ← arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913900,2080TA 5.1% 322.8 / 4.8 109D123V (GAT→GTT) ‡artJarginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (57/45);  new base A (2/4);  total (59/50)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 109 total aligned reads displayed.

AAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAC  >  NC_000913/900060‑900354
                                                                                                                                                    |                                                                                                                                                  
aaaTACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATc                                                                                                                                                   <  2:1609452/150‑1 (MQ=255)
aaaTACCCAATCAATACGAACATTTTTCATATCTATAAAGGCATTCTGATAACTGTCATAAGAGACTGTTTTCACTTCCGGGTGCTTATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCGATCCCAATACGTTTGCCTTTCAGATc                                                                                                                                                   <  1:388611/150‑1 (MQ=255)
  aTACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCgg                                                                                                                                                 <  1:553542/150‑1 (MQ=255)
     ccccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaa                                                                                                                                              >  2:539823/1‑150 (MQ=255)
        cATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACg                                                                                                                                           <  2:681827/150‑1 (MQ=255)
        cATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATAATTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACg                                                                                                                                           <  2:1714800/150‑1 (MQ=255)
           cAATACGACCATTTTTCAGATCGATAAAGGCATTCTTATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCAAACGtat                                                                                                                                        <  1:1061238/150‑3 (MQ=255)
           cAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGTTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGttt                                                                                                                                        >  2:270513/1‑150 (MQ=255)
           aaaTACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTCCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCAAACGtat                                                                                                                                        <  1:115999/149‑3 (MQ=255)
            aaTACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGtttt                                                                                                                                       <  2:558016/150‑1 (MQ=255)
            aaTACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCAAACGtatt                                                                                                                                       <  2:458658/150‑1 (MQ=255)
               aCGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTTAATATATTTCTGGTGCGTAGTAACGTTTTCCATCCCAATACGTTTGACTTTCAGATCGGCAAACGTTTTGTa                                                                                                                                    >  1:256158/1‑150 (MQ=255)
                cGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTTCCTTTCAGATCGGCAAACGTTTTCTAg                                                                                                                                   >  2:174393/1‑150 (MQ=255)
                cGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAg                                                                                                                                   <  1:1312579/150‑1 (MQ=255)
                 tatcATTTTTCAGATCTATAAAGTCATTCTGATAACTGTCATAAGAGTCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAgg                                                                                                                                  <  1:1603411/147‑1 (MQ=255)
                 gACCATTTTTCAGATCGATAAAGGCATTCTGATAAATGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAgg                                                                                                                                  <  1:918311/150‑1 (MQ=255)
                 gACCATTTTTCAGATCGAAAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCAAACGTATTgggat                                                                                                                                  <  2:350919/150‑5 (MQ=38)
                       ttttCAGATCGATAAAGGCTTTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtt                                                                                                                            <  1:449081/150‑1 (MQ=255)
                       ttttCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtt                                                                                                                            >  1:998905/1‑150 (MQ=255)
                         ttCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttt                                                                                                                          >  1:958265/1‑150 (MQ=255)
                           cAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                        >  2:194227/1‑150 (MQ=255)
                            aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTg                                                                                                                       <  2:1119665/150‑1 (MQ=255)
                             gATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                      >  1:1777941/1‑150 (MQ=255)
                             gATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAATACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                      <  2:386298/150‑1 (MQ=255)
                              aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgga                                                                                                                     >  2:653706/1‑149 (MQ=255)
                              aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGc                                                                                                                     <  1:1489065/150‑1 (MQ=255)
                              aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGc                                                                                                                     >  2:799118/1‑150 (MQ=255)
                               tCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCa                                                                                                                    <  2:462611/150‑1 (MQ=255)
                                 gATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAAt                                                                                                                  >  2:1351427/1‑150 (MQ=255)
                                   tAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCa                                                                                                                >  1:1045545/1‑150 (MQ=255)
                                     aaGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCacg                                                                                                              >  1:1498242/1‑150 (MQ=255)
                                      aGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTAACGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCacga                                                                                                             <  2:1056136/150‑1 (MQ=255)
                                          aTTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCATGACGGc                                                                                                         >  1:1843639/1‑150 (MQ=255)
                                              tGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAg                                                                                                     >  2:383678/1‑150 (MQ=255)
                                                  aaCTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGtttt                                                                                                 <  2:1060558/150‑1 (MQ=255)
                                                   aCTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTc                                                                                                <  1:195635/150‑1 (MQ=255)
                                                    cTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTGTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCa                                                                                               >  2:1477547/1‑150 (MQ=255)
                                                     tGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAt                                                                                              >  2:898534/1‑150 (MQ=255)
                                                       tCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAtag                                                                                            >  1:1571836/1‑150 (MQ=255)
                                                         aTAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAtagta                                                                                          >  1:566723/1‑150 (MQ=255)
                                                          tAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAtagtag                                                                                         <  1:1440909/150‑1 (MQ=255)
                                                           aaGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAgg                                                                                        >  2:1820861/1‑150 (MQ=255)
                                                            agagACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAggg                                                                                       >  2:1766210/1‑150 (MQ=255)
                                                            agagACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAggg                                                                                       <  2:1401330/150‑1 (MQ=255)
                                                            agagACAGTTTTCACTTACGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAggg                                                                                       <  2:1393711/150‑1 (MQ=255)
                                                               gaCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGt                                                                                    >  2:458385/1‑150 (MQ=255)
                                                               gaCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGt                                                                                    >  1:805642/1‑150 (MQ=255)
                                                               gaCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGt                                                                                    >  2:1498461/1‑150 (MQ=255)
                                                               gaCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGt                                                                                    >  2:572586/1‑150 (MQ=255)
                                                                 aaGTTTTCAATTCCGGGTGCTGCTCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATTCGTTTGCCTTTCAGATCGTCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTgg                                                                                  <  2:177432/149‑1 (MQ=255)
                                                                   gTTTTCATTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGCCGGCTGAGTTTTCATAGTAGGGCGTGGTa                                                                                >  2:1386561/1‑150 (MQ=255)
                                                                   gTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTATGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTa                                                                                >  2:1274415/1‑150 (MQ=255)
                                                                     tttCACTTCCGGGTGCTGCTCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGGTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTaaa                                                                              >  2:512231/1‑150 (MQ=255)
                                                                      ttCACTTCCGGGTGCTGATCCTGAATATATTCCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTAAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACTGCTGAGTTTTCTTAGTAGGTCGTGGTaatc                                                                             >  1:160093/1‑148 (MQ=255)
                                                                          cTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATa                                                                         <  1:1901945/150‑1 (MQ=255)
                                                                            tCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATAcc                                                                       <  2:1859979/150‑1 (MQ=255)
                                                                            tCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATAcc                                                                       >  1:1229945/1‑150 (MQ=255)
                                                                                ggTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGGGTTTTCATAGTAGGGCGTGGTAAACGATACCTGtt                                                                   <  2:1886149/150‑1 (MQ=255)
                                                                                ggTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGATAAACGATACCTGtt                                                                   <  1:1002104/150‑1 (MQ=255)
                                                                                  tGCTGATCCTGAATATATTTATGGTGCGTAGTCCCGGTGTCCCTCCCAATACGTTTGCCTTTCTGATCTGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGCCTGAGTTTTCATAGTAGGGCGGGGTAAACGATACCTGTTTg                                                                 <  2:307444/150‑1 (MQ=255)
                                                                                    cTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAATCGTTTTGTAGGTATCTTTTTTGGCAATCACGACGTCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCt                                                               >  1:314976/1‑150 (MQ=255)
                                                                                     tGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                              >  1:676597/1‑150 (MQ=255)
                                                                                      gATCCTTAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTAc                                                             <  1:1790268/150‑1 (MQ=255)
                                                                                           tGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTGAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCAAGACGGCTGAGTTTTCATAGTAGGGCGTTGTAAACGATACCTGTTTGCTACGCTcc                                                        >  1:357108/1‑150 (MQ=255)
                                                                                            gaattaatTTCTAGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGGGGTAAACGATACCTGTTTGCTACGCTCCg                                                       <  1:101393/144‑1 (MQ=255)
                                                                                            gAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCg                                                       >  1:135339/1‑150 (MQ=255)
                                                                                            gAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCg                                                       <  1:146646/150‑1 (MQ=255)
                                                                                            gAATAGATTTCTGGAGCGTAGTACAGTTTTCCATCCCTATACGTGTGCCTATCAGCGCGGCAAACGTTTTGTATGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGGGTGGTCAACGATACCTGTTTGCTACGCTCCg                                                       <  1:105909/150‑1 (MQ=255)
                                                                                              atatatTTCTGGTGCGTAGTACGGTTTGCCTTCCCAATACGGTTGCCTTTCAGATCGGAAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCggg                                                     <  2:1683571/150‑1 (MQ=255)
                                                                                                atatTTCTGGTGCGTAGTACCGTTTTCCATCCCAATATGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGt                                                   <  2:538623/150‑1 (MQ=255)
                                                                                                atatTTCTGGTGAGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGt                                                   <  1:1168560/150‑1 (MQ=255)
                                                                                                      cTGGTGCGTAGTACCGGTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGTTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATc                                             <  1:828806/150‑1 (MQ=255)
                                                                                                        ggTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGTGGTGATATCCa                                           >  1:1403667/1‑150 (MQ=255)
                                                                                                        ggTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCa                                           >  2:946301/1‑150 (MQ=255)
                                                                                                        ggTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCa                                           >  2:401682/1‑150 (MQ=255)
                                                                                                           gCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATAc                                        >  1:1694508/1‑150 (MQ=255)
                                                                                                             gtagtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTATACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCg                                      >  2:44719/1‑150 (MQ=255)
                                                                                                              tagtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCgg                                     <  1:1094645/150‑1 (MQ=255)
                                                                                                               agtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGga                                    <  1:539823/150‑1 (MQ=255)
                                                                                                                 taCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGgaga                                  >  1:803830/1‑150 (MQ=255)
                                                                                                                   ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGAtt                                >  1:1156310/1‑150 (MQ=255)
                                                                                                                   ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGAtt                                >  1:951540/1‑150 (MQ=255)
                                                                                                                   ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGAtt                                >  1:1831575/1‑150 (MQ=255)
                                                                                                                     gTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTAc                              >  1:885472/1‑150 (MQ=255)
                                                                                                                      ttttCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACg                             <  1:1338762/150‑1 (MQ=255)
                                                                                                                       tttCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACgg                            <  2:1777941/150‑1 (MQ=255)
                                                                                                                         tCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCg                          <  1:458385/150‑1 (MQ=255)
                                                                                                                         tCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCg                          >  1:1261104/1‑150 (MQ=255)
                                                                                                                           cATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTc                        <  2:1214783/150‑1 (MQ=255)
                                                                                                                               ccAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCatat                    >  2:1809884/1‑150 (MQ=255)
                                                                                                                               acAAAACGTTTGCCGATCTGAAAGGCAAACGTATTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAATCGATACCTGTTTGCTACGCTCCGGGGTGATATCCATTCCGGAGATTTCGGCGTCatat                    >  1:1303216/2‑150 (MQ=38)
                                                                                                                                cAAAACGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTATTCATAGTACGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATAtt                   >  1:1256573/1‑150 (MQ=255)
                                                                                                                                     cGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTg              >  1:1665711/1‑150 (MQ=255)
                                                                                                                                       tttGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGaa            >  2:1855465/1‑150 (MQ=255)
                                                                                                                                           cgtttCTGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTc        <  2:1498242/148‑1 (MQ=255)
                                                                                                                                              ttCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAgg     <  1:676242/150‑1 (MQ=255)
                                                                                                                                                cAGATCGGCAAACGTTTTGTAGGTATCTTATTTGGCAATAACGACGGATGAGATTACATAGTAGGGCGTGGTAAACGATACCTGTTTTCTAATCTCCTGGGTGATATCCATACAGGAGATTAATGCCTCATATTTTATGAATATCAggtt   >  1:1474/1‑148 (MQ=255)
                                                                                                                                                 aGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAc  >  2:146796/1‑150 (MQ=255)
                                                                                                                                                 aGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAc  >  2:806295/1‑150 (MQ=255)
                                                                                                                                                 aGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGATTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACTGCGTCATATTTTCTGAATTTCAGGGAc  >  1:1409020/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAC  >  NC_000913/900060‑900354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑