Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 987,560 G→C 10.2% intergenic (‑578/+25) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913987,5600GC10.2% 352.7 / 29.5 118intergenic (‑578/+25)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (56/50);  new base C (6/6);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-02

Only 100 of 119 total aligned reads displayed.

ATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACC  >  NC_000913/987411‑987707
                                                                                                                                                     |                                                                                                                                                   
aTCCGCAATAAGAATGCGTATTAGTTTTTGCGAGAAAATGTTGCAATGTAATATTACGTAACAGGATGATCGATATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACTGTTAACATGACAGAAAAGATAAAAAAAGAGCCAGCgg                                                                                                                                                     <  1:322347/150‑1 (MQ=255)
   cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGTGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  <  2:368453/150‑1 (MQ=255)
   cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGTGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  <  2:363034/150‑1 (MQ=255)
   cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  >  2:19925/1‑150 (MQ=255)
        tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGc                                                                                                                                             >  2:1576047/1‑150 (MQ=255)
        tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGc                                                                                                                                             <  2:1125166/150‑1 (MQ=255)
          aGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGctc                                                                                                                                           >  2:1526140/1‑150 (MQ=255)
           taaTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGctct                                                                                                                                          <  2:126170/149‑1 (MQ=255)
           gAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGctct                                                                                                                                          <  1:1036975/150‑1 (MQ=255)
            aaTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACATAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCtt                                                                                                                                         <  1:831369/150‑1 (MQ=255)
                cGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCGTGAAg                                                                                                                                     >  2:1654672/1‑150 (MQ=255)
                cGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGAAACAGTATGATCGTTATCGCGGGTTTGCAAATACATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGCTCtttttt                                                                                                                                     <  1:211358/150‑5 (MQ=255)
                 ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAGCCAGCGGGCGCTGGCTCTTGAAGa                                                                                                                                    <  1:876116/150‑1 (MQ=255)
                 ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGa                                                                                                                                    >  2:719849/1‑150 (MQ=255)
                 ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGCTCttttttt                                                                                                                                    <  1:100976/150‑6 (MQ=255)
                  gATTAGTTTTTGCGCGTAAATGTTGCTATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGAAAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGAc                                                                                                                                   >  2:1093189/1‑150 (MQ=255)
                  gATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGAc                                                                                                                                   >  1:457691/1‑150 (MQ=255)
                      aGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGCCGCTGGCTCTTGAAGACGCTa                                                                                                                               <  1:50209/150‑1 (MQ=255)
                       ttttttGAGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCTGTGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTaa                                                                                                                              <  2:1895010/149‑1 (MQ=255)
                       gTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTaa                                                                                                                              <  1:1308141/150‑1 (MQ=255)
                        tttttGCGCTTAAATGTTGAAATGTAATAATCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAGCCAGCGGGCGCGGGCTCTTGAAGACGCTaaa                                                                                                                             <  1:266783/150‑1 (MQ=255)
                         ttttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAAt                                                                                                                            <  1:706438/150‑1 (MQ=255)
                           ttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTa                                                                                                                          <  1:1216797/150‑1 (MQ=255)
                             gcgcGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGa                                                                                                                        <  2:1866166/150‑1 (MQ=255)
                              cgcgTAAATGTTGCAATGTAATATTCCGTAACAGGTTGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGaa                                                                                                                       <  1:1633600/150‑1 (MQ=255)
                               gcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAg                                                                                                                      >  2:1543606/1‑150 (MQ=255)
                                cgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGc                                                                                                                     >  1:227576/1‑150 (MQ=255)
                                   aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTgg                                                                                                                  >  2:1480048/1‑150 (MQ=255)
                                      tGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGt                                                                                                               <  2:375384/150‑1 (MQ=255)
                                       gTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGtt                                                                                                              >  1:1605166/1‑150 (MQ=255)
                                       gTTGAAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTACAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGtt                                                                                                              <  1:490656/150‑1 (MQ=255)
                                          gCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAACATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAcg                                                                                                           <  1:1605268/150‑1 (MQ=255)
                                           gaaTGTAATATTCAGTAACAGGATGATCGTTATGGCGGGGTTGCTAATAGATTGCTTGACTTAACAAACAGATAAGTTGACAGTATAGATAAAAAATGAGCCAGCGGGGGCTGGGTCTTGATGACGCTAAATTAGAAGCTGGCGTTAcgc                                                                                                          <  2:781520/149‑1 (MQ=255)
                                           cAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAcgc                                                                                                          >  1:1732387/1‑150 (MQ=255)
                                             aTGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAATATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCgg                                                                                                        <  1:842469/150‑1 (MQ=255)
                                                tAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGt                                                                                                     >  2:228728/1‑150 (MQ=255)
                                                tAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGt                                                                                                     >  2:1804407/1‑150 (MQ=255)
                                                      tCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTgg                                                                                               <  1:869976/150‑1 (MQ=255)
                                                        cGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGa                                                                                             >  2:770081/1‑150 (MQ=255)
                                                           aaCAGGATGATCGTTATGGCGGGGTTGCAACTAGTTTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAACAGCGCCAGCGGGCGCGGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          <  2:1013405/150‑1 (MQ=255)
                                                           aaCAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          <  2:1504326/150‑1 (MQ=255)
                                                             cAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGa                                                                                        >  2:1576329/1‑150 (MQ=255)
                                                                      cGTTATGGCGGGGTTTCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatcacatca                                                                               <  2:1748959/150‑1 (MQ=255)
                                                                       gTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          >  2:327766/1‑138 (MQ=255)
                                                                        ttctGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacg                                                                             <  2:489917/147‑1 (MQ=255)
                                                                          aTGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgta                                                                           >  2:851531/1‑150 (MQ=255)
                                                                           tGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCATATCacgtac                                                                          >  2:2076/1‑150 (MQ=255)
                                                                            ggCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgtacg                                                                         >  1:791060/1‑150 (MQ=255)
                                                                                  gTTGCAAATAGATTGCTTGCCTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAGCCAGCGGGCGCTGGCTCTTGACGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAAGCACATCACGTACGTTTTGc                                                                   >  2:964924/1‑150 (MQ=255)
                                                                                  gTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGc                                                                   <  2:714353/150‑1 (MQ=255)
                                                                                  gTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGc                                                                   >  2:599178/1‑150 (MQ=255)
                                                                                   ttGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCa                                                                  <  2:492399/150‑1 (MQ=255)
                                                                                     gCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACg                                                                >  1:81931/1‑150 (MQ=255)
                                                                                      cAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGc                                                               >  1:813915/1‑150 (MQ=255)
                                                                                      cAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGc                                                               >  1:1499622/1‑150 (MQ=255)
                                                                                      cAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGc                                                               >  2:932448/1‑150 (MQ=255)
                                                                                       aaaTAGATTGCTTGACGTAACAAACAGATAACTTGACCGAAAAGATAAAACACGAGCCAGCGGGCGCTGGCTCGTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGcc                                                              <  2:917184/150‑1 (MQ=255)
                                                                                            taTTGCTTGACTTACCACACAGATAACTTGACAGAAAAGATAAAAAACGTGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTa                                                         <  2:689175/149‑1 (MQ=255)
                                                                                                gCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACTCGGAGTACGTCGTAACTGAATCACATAACGTACGTTTTGCACGCCAGTTACGTa                                                     >  1:1177758/1‑150 (MQ=255)
                                                                                                gCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTa                                                     >  2:572242/1‑150 (MQ=255)
                                                                                                   tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc                                                  >  1:1610310/1‑150 (MQ=255)
                                                                                                   tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc                                                  >  1:1714911/1‑150 (MQ=255)
                                                                                                   tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTCGCACGCCAGTTACGTAAGc                                                  <  1:717645/150‑1 (MQ=255)
                                                                                                   ggACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc                                                  <  1:1420615/149‑1 (MQ=255)
                                                                                                    gACTTAACAAACAGAAAACATGACATAAAAGATAAAAACAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAAGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACTTAAGCa                                                 <  1:1099912/150‑1 (MQ=255)
                                                                                                       ttAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATc                                              <  2:1760893/150‑1 (MQ=255)
                                                                                                        tAACAAACAGATAACTTGACGGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAAGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCa                                             >  1:1544633/1‑150 (MQ=255)
                                                                                                        tAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCa                                             <  2:36424/150‑1 (MQ=255)
                                                                                                         aacaaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAg                                            >  1:1751207/1‑150 (MQ=255)
                                                                                                           caaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGAc                                          >  1:1761912/1‑150 (MQ=255)
                                                                                                            aaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACg                                         <  1:489826/150‑1 (MQ=255)
                                                                                                              acaGATAACTTGACAGAAAATATAAAAAAAGAGCAAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGCAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGtt                                       <  2:307075/150‑1 (MQ=255)
                                                                                                               caGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTc                                      <  2:776086/150‑1 (MQ=255)
                                                                                                                  aTAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCaaa                                   <  2:1803155/150‑1 (MQ=255)
                                                                                                                  aTAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCaaa                                   <  2:505767/150‑1 (MQ=255)
                                                                                                                      cTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGATTCACATCACGTACGTTTTGCACGGTAGTTACGTAAGAAATCAGAGGTTCATCACCa                               >  2:347825/1‑150 (MQ=255)
                                                                                                                        tGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAg                             >  1:746174/1‑150 (MQ=255)
                                                                                                                         taCAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGa                            <  1:1576545/149‑1 (MQ=255)
                                                                                                                          aCAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGAc                           >  1:413391/1‑150 (MQ=255)
                                                                                                                          aCAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGAc                           >  1:1831974/1‑150 (MQ=255)
                                                                                                                            aGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGATGCTGGCGTTACGCGGAGTTCGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGGTCAAAACCAAGaacc                         >  2:100625/1‑147 (MQ=255)
                                                                                                                            aGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCCCGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCg                         >  2:1139312/1‑150 (MQ=255)
                                                                                                                               aaaGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGaaa                      <  2:227576/150‑1 (MQ=255)
                                                                                                                                   aTAAAAAAAGAGCCAGTGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCAGGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTg                  <  2:1751347/150‑1 (MQ=255)
                                                                                                                                   aTAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTg                  >  1:1485083/1‑150 (MQ=255)
                                                                                                                                    tAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGa                 <  1:1526140/150‑1 (MQ=255)
                                                                                                                                    tAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGATACGTAAGCAATCAGCCGTTCAAAACCAAGACCGCAACCTGa                 >  2:83576/1‑150 (MQ=255)
                                                                                                                                    gtcttcaaGAGCCAGCGCCCGCTGGCTCTTGAAGACGCTTAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGa                 >  2:1508993/7‑150 (MQ=255)
                                                                                                                                    gtcttcaaGAGCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAACAATCAGACGTTGAACACCAAGCCCGAAACCTGa                 >  1:1070234/7‑150 (MQ=255)
                                                                                                                                     aaaaaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGaa                >  1:115938/1‑150 (MQ=255)
                                                                                                                                      cttcaaGAGCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAAt               >  2:1870517/5‑150 (MQ=255)
                                                                                                                                      aaaaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAAt               <  1:1137436/150‑1 (MQ=255)
                                                                                                                                       aaaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATg              >  1:104831/1‑150 (MQ=255)
                                                                                                                                        aaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACCTCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAACACAAAGACCGAAACCTGAATGc             >  2:165175/1‑150 (MQ=255)
                                                                                                                                        aaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCAGGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGc             >  1:1226860/1‑150 (MQ=255)
                                                                                                                                          aaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTGCGCGGAGTACGTGGGACCGGAATCACATCAACTACGTTTTGCACGCCAGTTACGTAAGCAATCAGCCGTTCAAAACCAAGACCGAAACCTGATTGCgg           >  1:93174/1‑150 (MQ=255)
                                                                                                                                           agagCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGa          >  2:1706802/1‑150 (MQ=255)
                                                                                                                                           agagCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGAAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGa          >  1:1083961/1‑150 (MQ=255)
                                                                                                                                             agCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAAc        <  2:700037/150‑1 (MQ=255)
                                                                                                                                                   cGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTAcc  <  1:19925/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACC  >  NC_000913/987411‑987707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: