Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 987,561 G→C 12.2% intergenic (‑579/+24) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913987,5610GC12.2% 343.4 / 30.4 113intergenic (‑579/+24)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (52/47);  new base C (7/7);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 118 total aligned reads displayed.

CGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACC  >  NC_000913/987414‑987707
                                                                                                                                                   |                                                                                                                                                  
cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGTGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  <  2:368453/150‑1 (MQ=255)
cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGTGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  <  2:363034/150‑1 (MQ=255)
cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  >  2:19925/1‑150 (MQ=255)
cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                                                                  >  1:1241576/1‑150 (MQ=255)
 gCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcga                                                                                                                                                 >  1:825512/1‑149 (MQ=255)
     tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGc                                                                                                                                             >  2:1576047/1‑150 (MQ=255)
     tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGc                                                                                                                                             <  2:1125166/150‑1 (MQ=255)
       aGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGctc                                                                                                                                           >  2:1526140/1‑150 (MQ=255)
        taaTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGctct                                                                                                                                          <  2:126170/149‑1 (MQ=255)
        gAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGctct                                                                                                                                          <  1:1036975/150‑1 (MQ=255)
         aaTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACATAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCtt                                                                                                                                         <  1:831369/150‑1 (MQ=255)
          aTGCGGTTTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTg                                                                                                                                        <  1:987569/150‑1 (MQ=255)
           tGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGCTCtttt                                                                                                                                       <  2:1226858/150‑3 (MQ=255)
            gCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTACCAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGCCGCTGGCTCATGaa                                                                                                                                      >  2:174878/1‑150 (MQ=255)
             cGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGAAACAGTATGATCGTTATCGCGGGTTTGCAAATACATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGCTCtttttt                                                                                                                                     <  1:211358/150‑5 (MQ=255)
              ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAGCCAGCGGGCGCTGGCTCTTGAAGa                                                                                                                                    <  1:876116/150‑1 (MQ=255)
              ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGa                                                                                                                                    >  2:719849/1‑150 (MQ=255)
              ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGCCCGCTGGCTCttttttt                                                                                                                                    <  1:100976/150‑6 (MQ=255)
               gATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGAc                                                                                                                                   >  1:457691/1‑150 (MQ=255)
                   aGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGCCGCTGGCTCTTGAAGACGCTa                                                                                                                               <  1:50209/150‑1 (MQ=255)
                    ttttttGAGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCTGTGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTaa                                                                                                                              <  2:1895010/149‑1 (MQ=255)
                     tttttGCGCTTAAATGTTGAAATGTAATAATCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAGCCAGCGGGCGCGGGCTCTTGAAGACGCTaaa                                                                                                                             <  1:266783/150‑1 (MQ=255)
                      ttttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAAt                                                                                                                            <  1:706438/150‑1 (MQ=255)
                        ttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTa                                                                                                                          <  1:1216797/150‑1 (MQ=255)
                          gcgcGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGa                                                                                                                        <  2:1866166/150‑1 (MQ=255)
                           cgcgTAAATGTTGCAATGTAATATTCCGTAACAGGTTGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGaa                                                                                                                       <  1:1633600/150‑1 (MQ=255)
                            gcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAg                                                                                                                      >  2:1543606/1‑150 (MQ=255)
                             cgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGc                                                                                                                     >  1:227576/1‑150 (MQ=255)
                                aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTgg                                                                                                                  >  2:1480048/1‑150 (MQ=255)
                                   tGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGt                                                                                                               <  2:375384/150‑1 (MQ=255)
                                    gTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGtt                                                                                                              >  1:1605166/1‑150 (MQ=255)
                                    gTTGAAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTACAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGtt                                                                                                              <  1:490656/150‑1 (MQ=255)
                                       gCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAACATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAcg                                                                                                           <  1:1605268/150‑1 (MQ=255)
                                        gaaTGTAATATTCAGTAACAGGATGATCGTTATGGCGGGGTTGCTAATAGATTGCTTGACTTAACAAACAGATAAGTTGACAGTATAGATAAAAAATGAGCCAGCGGGGGCTGGGTCTTGATGACGCTAAATTAGAAGCTGGCGTTAcgc                                                                                                          <  2:781520/149‑1 (MQ=255)
                                        cAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAcgc                                                                                                          >  1:1732387/1‑150 (MQ=255)
                                          aTGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACATATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGATGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCgg                                                                                                        <  1:1680296/150‑1 (MQ=255)
                                          aTGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAATATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCgg                                                                                                        <  1:842469/150‑1 (MQ=255)
                                             tAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGt                                                                                                     >  2:228728/1‑150 (MQ=255)
                                             tAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGt                                                                                                     >  2:1804407/1‑150 (MQ=255)
                                                    ccGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTggg                                                                                              >  2:501631/1‑150 (MQ=255)
                                                     cGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGa                                                                                             >  2:770081/1‑150 (MQ=255)
                                                        aaCAGGATGATCGTTATGGCGGGGTTGCAACTAGTTTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAACAGCGCCAGCGGGCGCGGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          <  2:1013405/150‑1 (MQ=255)
                                                        aaCAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          <  2:1504326/150‑1 (MQ=255)
                                                          cAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGa                                                                                        >  2:1576329/1‑150 (MQ=255)
                                                                gatCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatcaca                                                                                  >  1:703355/1‑150 (MQ=255)
                                                                   cGTTATGGCGGGGTTTCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatcacatca                                                                               <  2:1748959/150‑1 (MQ=255)
                                                                    gTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          >  2:327766/1‑138 (MQ=255)
                                                                    gTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACg                                                                                          <  1:327766/138‑1 (MQ=255)
                                                                     ttctGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacg                                                                             <  2:489917/147‑1 (MQ=255)
                                                                       aTGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgta                                                                           >  2:851531/1‑150 (MQ=255)
                                                                         ggCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgtacg                                                                         >  1:791060/1‑150 (MQ=255)
                                                                               gTTGCAAATAGATTGCTTGCCTTAACAAACAGATAACTTGACAGAAAAGATAAAAAACGAGCCAGCGGGCGCTGGCTCTTGACGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAAGCACATCACGTACGTTTTGc                                                                   >  2:964924/1‑150 (MQ=255)
                                                                               gTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGc                                                                   <  2:714353/150‑1 (MQ=255)
                                                                               gTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGc                                                                   <  2:714292/150‑1 (MQ=255)
                                                                               gTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGc                                                                   >  2:599178/1‑150 (MQ=255)
                                                                                  gCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACg                                                                >  1:81931/1‑150 (MQ=255)
                                                                                   cAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGc                                                               >  2:932448/1‑150 (MQ=255)
                                                                                   cAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGc                                                               >  1:813915/1‑150 (MQ=255)
                                                                                   cAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGc                                                               >  1:1499622/1‑150 (MQ=255)
                                                                                    aaaTAGATTGCTTGACGTAACAAACAGATAACTTGACCGAAAAGATAAAACACGAGCCAGCGGGCGCTGGCTCGTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGcc                                                              <  2:917184/150‑1 (MQ=255)
                                                                                         taTTGCTTGACTTACCACACAGATAACTTGACAGAAAAGATAAAAAACGTGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTa                                                         <  2:689175/149‑1 (MQ=255)
                                                                                             gCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACTCGGAGTACGTCGTAACTGAATCACATAACGTACGTTTTGCACGCCAGTTACGTa                                                     >  1:1177758/1‑150 (MQ=255)
                                                                                             gCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTa                                                     >  2:572242/1‑150 (MQ=255)
                                                                                                tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc                                                  >  1:1610310/1‑150 (MQ=255)
                                                                                                tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTCGCACGCCAGTTACGTAAGc                                                  <  1:717645/150‑1 (MQ=255)
                                                                                                ggACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc                                                  <  1:1420615/149‑1 (MQ=255)
                                                                                                 gACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCCGCGGGCGCTGGCTCTTGAAGACGCTAAAATAGAAGCGGGCGTTACTCGGAGGACGTGGGGACGGAATCCCATCACGTACGTTTTGCACGCCAGTTACGTAAGCa                                                 >  2:361163/1‑150 (MQ=255)
                                                                                                 gACTTAACAAACAGAAAACATGACATAAAAGATAAAAACAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTAAGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACTTAAGCa                                                 <  1:1099912/150‑1 (MQ=255)
                                                                                                    ttAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATc                                              <  2:1760893/150‑1 (MQ=255)
                                                                                                     tAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCa                                             <  2:36424/150‑1 (MQ=255)
                                                                                                      aacaaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAg                                            >  1:1751207/1‑150 (MQ=255)
                                                                                                        caaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGAc                                          >  1:1761912/1‑150 (MQ=255)
                                                                                                           acaGATAACTTGACAGAAAATATAAAAAAAGAGCAAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGCAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGtt                                       <  2:307075/150‑1 (MQ=255)
                                                                                                            caGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTc                                      <  2:776086/150‑1 (MQ=255)
                                                                                                               aTAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCaaa                                   <  2:505767/150‑1 (MQ=255)
                                                                                                                  aCTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAAcc                                >  2:1543416/1‑150 (MQ=255)
                                                                                                                      taCAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGa                            <  1:1576545/149‑1 (MQ=255)
                                                                                                                       aCAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGAc                           >  1:413391/1‑150 (MQ=255)
                                                                                                                       aCAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGAc                           >  1:1831974/1‑150 (MQ=255)
                                                                                                                         aGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGATGCTGGCGTTACGCGGAGTTCGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGGTCAAAACCAAGaacc                         >  2:100625/1‑147 (MQ=255)
                                                                                                                         aGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCCCGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCg                         >  2:1139312/1‑150 (MQ=255)
                                                                                                                         aGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACAACACGTACGTTTTGCACGCGAGTGACGTATGCAATCAGACGTTCATCCCCAAGCCCg                         >  1:153004/1‑150 (MQ=255)
                                                                                                                            aaaGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGaaa                      <  2:227576/150‑1 (MQ=255)
                                                                                                                                aTAAAAAAAGAGCCAGTGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCAGGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTg                  <  2:1751347/150‑1 (MQ=255)
                                                                                                                                aTAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTg                  >  1:1485083/1‑150 (MQ=255)
                                                                                                                                 tAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGa                 <  1:1526140/150‑1 (MQ=255)
                                                                                                                                 tAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGATACGTAAGCAATCAGCCGTTCAAAACCAAGACCGCAACCTGa                 >  2:83576/1‑150 (MQ=255)
                                                                                                                                 gtcttcaaGAGCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAACAATCAGACGTTGAACACCAAGCCCGAAACCTGa                 >  1:1070234/7‑150 (MQ=255)
                                                                                                                                  aaaaaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGaa                >  1:115938/1‑150 (MQ=255)
                                                                                                                                   cttcaaGAGCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAAt               >  2:1870517/5‑150 (MQ=255)
                                                                                                                                   aaaaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAAt               <  1:1137436/150‑1 (MQ=255)
                                                                                                                                    aaaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATg              >  1:104831/1‑150 (MQ=255)
                                                                                                                                     aaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACCTCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAACACAAAGACCGAAACCTGAATGc             >  2:165175/1‑150 (MQ=255)
                                                                                                                                     aaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCAGGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGc             >  1:1226860/1‑150 (MQ=255)
                                                                                                                                     aaaaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGc             >  2:21225/1‑150 (MQ=255)
                                                                                                                                       aaGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTGCGCGGAGTACGTGGGACCGGAATCACATCAACTACGTTTTGCACGCCAGTTACGTAAGCAATCAGCCGTTCAAAACCAAGACCGAAACCTGATTGCgg           >  1:93174/1‑150 (MQ=255)
                                                                                                                                        agagCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAACCCTGAATGCGGa          >  1:1862006/1‑150 (MQ=255)
                                                                                                                                        agagCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGa          >  2:1706802/1‑150 (MQ=255)
                                                                                                                                        agagCCAGCGCCCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGAAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGa          >  1:1083961/1‑150 (MQ=255)
                                                                                                                                            ccAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAg      <  2:1860944/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCGAAACCTGAATGCGGAACAGTACC  >  NC_000913/987414‑987707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: