Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,074,894 G→T 5.2% intergenic (+14/+26) rutR → / ← putA rut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,074,8940GT 5.2% 415.0 / 10.1 134intergenic (+14/+26)rutR/putArut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (65/62);  new base T (4/3);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

Only 100 of 140 total aligned reads displayed.

TCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAA  >  NC_000913/1074746‑1075043
                                                                                                                                                    |                                                                                                                                                     
tCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGaggagg                                                                                                                                                      >  1:1750319/1‑150 (MQ=255)
   aCTCATCATTACGCCGATTTCGCCCGTCAGGTGGAGGCGGTGAGAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGTCCACGTTAAAGATGCCGTAGGAGGTTg                                                                                                                                                   <  1:1220527/150‑1 (MQ=255)
   aCTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTg                                                                                                                                                   >  2:660427/1‑150 (MQ=255)
   aCTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTCAAAGATGCCGGAGGAGGTTg                                                                                                                                                   >  2:464196/1‑150 (MQ=255)
    cTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                                  >  2:516690/1‑150 (MQ=255)
    cTCAACATTACGCCGATTTCGCCCCTCAGGGGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGTTTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                                  <  1:1847391/150‑1 (MQ=255)
     tCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTa                                                                                                                                                 >  2:1302812/1‑150 (MQ=255)
      cAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTaa                                                                                                                                                <  1:1301783/150‑1 (MQ=255)
        aCATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACa                                                                                                                                              <  1:920011/150‑1 (MQ=255)
           ttACGCCGATTTCGCCCCTCAGGGGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcc                                                                                                                                           <  2:1273931/150‑1 (MQ=255)
                ccGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCgg                                                                                                                                      <  2:1339714/150‑1 (MQ=255)
                ccGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCgg                                                                                                                                      >  1:1879877/1‑150 (MQ=255)
                ccGATTTCGCCCCGCCGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGATGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCgg                                                                                                                                      <  2:326431/150‑1 (MQ=255)
                 cGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGc                                                                                                                                     <  1:1263865/150‑1 (MQ=255)
                       cGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCGGcatcttt                                                                                                                               <  2:894267/150‑7 (MQ=255)
                       cGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTg                                                                                                                               <  2:117137/150‑1 (MQ=255)
                        gCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGt                                                                                                                              >  1:1158607/1‑150 (MQ=255)
                        gCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGt                                                                                                                              >  1:1027817/1‑150 (MQ=255)
                         ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGtt                                                                                                                             <  2:784783/150‑1 (MQ=255)
                         ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGtt                                                                                                                             >  2:603894/1‑150 (MQ=255)
                           ccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTa                                                                                                                           >  1:906140/1‑150 (MQ=255)
                            cTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTaa                                                                                                                          >  1:706909/1‑150 (MQ=255)
                            cTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTaa                                                                                                                          <  2:290450/150‑1 (MQ=255)
                              cAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAcc                                                                                                                        >  2:632452/1‑150 (MQ=255)
                                       gCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAt                                                                                                               <  1:409476/150‑1 (MQ=255)
                                        cGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAtt                                                                                                              >  1:664759/1‑150 (MQ=255)
                                            gACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGc                                                                                                          <  1:1844504/150‑1 (MQ=255)
                                            gACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGc                                                                                                          <  2:1546076/150‑1 (MQ=255)
                                                 gcgcGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCg                                                                                                     <  2:766063/150‑1 (MQ=255)
                                                      aCGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTAcc                                                                                                <  2:680828/150‑1 (MQ=255)
                                                        gTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGGTAACGc                                                                                              <  1:430510/150‑1 (MQ=255)
                                                         ttGCGCGATGGGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGcc                                                                                             <  2:1751613/150‑1 (MQ=255)
                                                         ttGAGCGATGAGGTATTTTTCAATCAAACTGTTGAAAACGTGCATCGGATTATTATTGAGGGTATGAGACCACGTTAAAGATGCCGGAGGAGGTGGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGcc                                                                                             <  2:1513761/150‑1 (MQ=255)
                                                               gATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggc                                                                                       >  1:894668/1‑150 (MQ=255)
                                                                 tGAGGTAGTTTTCATTCATCCTGTGGACAACGTGCAGCGGATTTTTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGATGTAACCTCCTCCGGCTTCCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcag                                                                                     <  1:370585/150‑1 (MQ=255)
                                                                  gAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagc                                                                                    <  2:886437/150‑1 (MQ=255)
                                                                    ggTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTAAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                                  >  1:1695997/1‑150 (MQ=255)
                                                                    ggTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTAGTATTGAGGGGATTCGACCACGTTTAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTTACCTATAGTCATTAAGCTGGCGTTTCCGCcagcggcagcgg                                                                                  >  1:315812/1‑150 (MQ=255)
                                                                     gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGt                                                                                 <  1:947857/150‑1 (MQ=255)
                                                                      tATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTa                                                                                <  1:1302812/150‑1 (MQ=255)
                                                                       aTTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAt                                                                               >  1:1774474/1‑150 (MQ=255)
                                                                        tttttCTATCAAACGGGTGAAAACGTGCAGCGGATTATTATTGAGGTGATTCTACCAGGTTAAAGATGCCGGTGAGGTTGTAACATGCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTc                                                                             <  2:1663343/150‑1 (MQ=255)
                                                                         ttttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTc                                                                             <  1:1021634/150‑1 (MQ=255)
                                                                          tttCAATCATACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                            <  1:812367/150‑1 (MQ=255)
                                                                          tttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                            <  2:1628303/150‑1 (MQ=255)
                                                                           ttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTcac                                                                           >  2:1304006/1‑150 (MQ=255)
                                                                             caatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTcaccc                                                                         >  2:358459/1‑148 (MQ=255)
                                                                              aatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATTCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACt                                                                        >  1:1651059/1‑150 (MQ=255)
                                                                              aatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACt                                                                        >  2:1734352/1‑150 (MQ=255)
                                                                               atcaaACGGTGGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTc                                                                       <  1:951084/150‑1 (MQ=255)
                                                                                tcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                      >  1:720975/1‑150 (MQ=255)
                                                                                tcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                      >  2:628384/1‑150 (MQ=255)
                                                                                tcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                      <  2:1880605/150‑1 (MQ=255)
                                                                                  aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGc                                                                    >  2:172625/1‑150 (MQ=255)
                                                                                  aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGc                                                                    <  1:268507/150‑1 (MQ=255)
                                                                                  aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGCCAGCGGTATTCACACTCAGc                                                                    >  1:1833535/1‑150 (MQ=255)
                                                                                  aaaCGGTTGAAAACGTGAAGCGGATTATTAAGGAGTGGATTCGCCGACGGTAAAGATGCCGGAGGAGGTTGGAACATCCTGCGGCTACATGCTGACACTATAGTCATTACTCTGCCGTTACAGACACCGTCTGCGGTAGTCACACTcccc                                                                    >  2:808375/1‑147 (MQ=255)
                                                                                      ggTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCACCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAc                                                                <  1:434362/150‑1 (MQ=255)
                                                                                       gTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACg                                                               <  1:603894/150‑1 (MQ=255)
                                                                                         tGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCt                                                             >  2:1470042/1‑150 (MQ=255)
                                                                                          gAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                            >  2:120838/1‑150 (MQ=255)
                                                                                           aaaaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCg                                                           >  2:1321710/1‑150 (MQ=255)
                                                                                             aaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGat                                                         <  2:202687/150‑1 (MQ=255)
                                                                                             aaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGat                                                         <  1:667449/150‑1 (MQ=255)
                                                                                               cGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTTAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGatat                                                       <  1:481583/150‑1 (MQ=255)
                                                                                               cGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGatat                                                       <  2:1879877/150‑1 (MQ=255)
                                                                                                  gCAGCGGATTATTTTTGTGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACa                                                    <  2:1335623/150‑1 (MQ=255)
                                                                                                  gCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACa                                                    >  2:276916/1‑150 (MQ=255)
                                                                                                    aGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGc                                                  <  1:1852802/150‑1 (MQ=255)
                                                                                                       ggATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGt                                               >  1:697709/1‑150 (MQ=255)
                                                                                                       ggATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGATCGATATACAGCCGt                                               >  2:1151233/1‑150 (MQ=255)
                                                                                                        gATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGtt                                              <  1:1372049/150‑1 (MQ=255)
                                                                                                          ttattattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTcc                                            <  2:1502165/150‑1 (MQ=255)
                                                                                                            attattAAGGGGATTCGACCACGTTAAAGATGACGGAGGAGGTAGTAACATCCTCCGGCTACCTGTTTAACATATATACATTAAGCTCGCGTTACCGCAAGCGGCAGCGGTATTCACACTCAGAGAACGCTCGATATACAGCCGTTCCAg                                          >  1:807108/1‑150 (MQ=255)
                                                                                                             ttattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGa                                         >  2:759493/1‑150 (MQ=255)
                                                                                                                 tGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGa                                     <  2:903694/150‑1 (MQ=255)
                                                                                                                 tGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGa                                     <  2:567961/150‑1 (MQ=255)
                                                                                                                 tGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTGGTAACATCCTCCGGCTAACTGTTTAACCTATAGTCATTCAGCTGGCGGTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGa                                     <  2:567964/150‑1 (MQ=255)
                                                                                                                  gAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTACCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGat                                    <  2:1470157/150‑1 (MQ=255)
                                                                                                                       gATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGc                               >  1:313710/1‑150 (MQ=255)
                                                                                                                           cGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                           >  1:745511/1‑150 (MQ=255)
                                                                                                                           cGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                           >  1:1355385/1‑150 (MQ=255)
                                                                                                                            gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                          <  1:755973/150‑1 (MQ=255)
                                                                                                                            gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                          <  1:1581076/150‑1 (MQ=255)
                                                                                                                             aCCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGc                         <  1:1383334/150‑1 (MQ=255)
                                                                                                                                 cGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATACACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                     <  2:460659/150‑1 (MQ=255)
                                                                                                                                    tAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGc                  >  1:740587/1‑150 (MQ=255)
                                                                                                                                    tAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGc                  >  1:344454/1‑150 (MQ=255)
                                                                                                                                       ggtagCCGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCaaa               >  1:1210228/5‑150 (MQ=255)
                                                                                                                                          tGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGTGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAcc            >  1:1698143/1‑150 (MQ=255)
                                                                                                                                          tGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAcc            >  1:963739/1‑150 (MQ=255)
                                                                                                                                           gCCGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAccc           >  2:960631/1‑150 (MQ=255)
                                                                                                                                           gCCGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCAATAGTCATTAAGCTGGCGTTACCTCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAccc           >  2:698489/1‑150 (MQ=255)
                                                                                                                                           gCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAAGCTCGATATACCGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGcttcaccc           >  2:1244652/1‑143 (MQ=255)
                                                                                                                                            ccGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCCGCGGCAGCGGTAGTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCTCGGGCAGAGcccg          >  1:114389/1‑149 (MQ=255)
                                                                                                                                            ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          <  2:883191/150‑1 (MQ=255)
                                                                                                                                            ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          >  1:374067/1‑150 (MQ=255)
                                                                                                                                            ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          >  1:661551/1‑150 (MQ=255)
                                                                                                                                              ggaggaGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGc        >  1:727708/1‑150 (MQ=255)
                                                                                                                                                aggaggTTGAAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGAGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAc      >  1:1106701/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAA  >  NC_000913/1074746‑1075043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: