Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,074,898 G→A 5.0% intergenic (+18/+22) rutR → / ← putA rut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,074,8980GA 5.0% 454.6 / 7.3 139intergenic (+18/+22)rutR/putArut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (66/65);  new base A (4/3);  total (71/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAA  >  NC_000913/1074749‑1075047
                                                                                                                                                     |                                                                                                                                                     
aCTCATCATTACGCCGATTTCGCCCGTCAGGTGGAGGCGGTGAGAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGTCCACGTTAAAGATGCCGTAGGAGGTTg                                                                                                                                                       <  1:1220527/150‑1 (MQ=255)
aCTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTCAAAGATGCCGGAGGAGGTTg                                                                                                                                                       >  2:464196/1‑150 (MQ=255)
 cTCAACATTACGCCGATTTCGCCCCTCAGGGGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGTTTCGACCACGTTAAAGATGCCGGAGGAGGTTGt                                                                                                                                                      <  1:1847391/150‑1 (MQ=255)
  tCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTa                                                                                                                                                     <  1:1866271/150‑1 (MQ=255)
   cAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTaa                                                                                                                                                    <  1:1301783/150‑1 (MQ=255)
     aCATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACa                                                                                                                                                  >  2:18955/1‑150 (MQ=255)
        ttACGCCGATTTCGCCCCTCAGGGGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcc                                                                                                                                               <  2:1273931/150‑1 (MQ=255)
             ccGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCgg                                                                                                                                          <  2:1339714/150‑1 (MQ=255)
             ccGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCgg                                                                                                                                          >  1:1879877/1‑150 (MQ=255)
             ccGATTTCGCCCCGCCGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGATGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCgg                                                                                                                                          <  2:326431/150‑1 (MQ=255)
                    cGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCGGcatcttt                                                                                                                                   <  2:894267/150‑7 (MQ=255)
                    cGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTg                                                                                                                                   <  2:117137/150‑1 (MQ=255)
                     gCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGt                                                                                                                                  >  1:1027817/1‑150 (MQ=255)
                     gCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGt                                                                                                                                  >  1:1158607/1‑150 (MQ=255)
                      ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGtt                                                                                                                                 >  2:603894/1‑150 (MQ=255)
                      ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGtt                                                                                                                                 >  2:1477887/1‑150 (MQ=255)
                       cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGttt                                                                                                                                >  1:408329/1‑150 (MQ=255)
                        ccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTa                                                                                                                               >  1:906140/1‑150 (MQ=255)
                         cTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTaa                                                                                                                              >  1:706909/1‑150 (MQ=255)
                         cTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTaa                                                                                                                              <  2:290450/150‑1 (MQ=255)
                           cAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAcc                                                                                                                            >  2:632452/1‑150 (MQ=255)
                             ggTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCta                                                                                                                          >  1:903694/1‑150 (MQ=255)
                                 gAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGt                                                                                                                      <  2:602726/150‑1 (MQ=255)
                                    gCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAt                                                                                                                   <  1:409476/150‑1 (MQ=255)
                                     cGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAtt                                                                                                                  >  1:1687241/1‑150 (MQ=255)
                                     cGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAtt                                                                                                                  >  1:664759/1‑150 (MQ=255)
                                         gACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGc                                                                                                              <  1:1844504/150‑1 (MQ=255)
                                         gACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGc                                                                                                              <  2:1546076/150‑1 (MQ=255)
                                           cAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAACTCGTGGAGATGATTATTAGTGATGGGATTCGCCAACGGTAAAGATGCCGGAGGAGGAATTAACATCCTCGCTCTACCTGTGTACCAGATAGGAATTAATCtt                                                                                                            >  2:1312202/1‑149 (MQ=255)
                                              gcgcGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCTGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCGGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATTGTCATTAAGCTGGCg                                                                                                         <  2:1176010/150‑1 (MQ=255)
                                              gcgcGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCg                                                                                                         <  2:766063/150‑1 (MQ=255)
                                                   aCGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTAcc                                                                                                    <  2:680828/150‑1 (MQ=255)
                                                     gTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGGTAACGc                                                                                                  <  1:430510/150‑1 (MQ=255)
                                                      ttGCGCGATGGGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGcc                                                                                                 <  2:1751613/150‑1 (MQ=255)
                                                          gcgATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcg                                                                                             <  1:1454224/150‑1 (MQ=255)
                                                           cgATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcgg                                                                                            >  1:1291366/1‑150 (MQ=255)
                                                              tGAGGTAGTTTTCATTCATCCTGTGGACAACGTGCAGCGGATTTTTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGATGTAACCTCCTCCGGCTTCCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcag                                                                                         <  1:370585/150‑1 (MQ=255)
                                                               gAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagc                                                                                        <  2:886437/150‑1 (MQ=255)
                                                                 ggTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                                      >  1:462230/1‑150 (MQ=255)
                                                                 ggTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                                      <  2:1302765/150‑1 (MQ=255)
                                                                 ggTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTAAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcgg                                                                                      >  1:1695997/1‑150 (MQ=255)
                                                                 ggTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTAGTATTGAGGGGATTCGACCACGTTTAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTTACCTATAGTCATTAAGCTGGCGTTTCCGCcagcggcagcgg                                                                                      >  1:315812/1‑150 (MQ=255)
                                                                  gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGt                                                                                     <  2:873417/150‑1 (MQ=255)
                                                                  gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGt                                                                                     <  1:947857/150‑1 (MQ=255)
                                                                  gTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGAGTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGTCGTTACCGCCAGCGGCAGCGGt                                                                                     >  1:346088/1‑150 (MQ=255)
                                                                   tATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTa                                                                                    <  1:1302812/150‑1 (MQ=255)
                                                                    aTTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAt                                                                                   >  1:1774474/1‑150 (MQ=255)
                                                                     tttttCTATCAAACGGGTGAAAACGTGCAGCGGATTATTATTGAGGTGATTCTACCAGGTTAAAGATGCCGGTGAGGTTGTAACATGCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTc                                                                                 <  2:1663343/150‑1 (MQ=255)
                                                                      ttttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTc                                                                                 <  1:1021634/150‑1 (MQ=255)
                                                                       tttCAATCATACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                                <  1:812367/150‑1 (MQ=255)
                                                                        ttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTcac                                                                               >  2:1304006/1‑150 (MQ=255)
                                                                          caatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTcaccc                                                                             >  2:358459/1‑148 (MQ=255)
                                                                          caatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCacac                                                                             >  2:1604687/1‑150 (MQ=255)
                                                                          caatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCacac                                                                             <  2:686972/150‑1 (MQ=255)
                                                                           aatcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACt                                                                            >  2:1734352/1‑150 (MQ=255)
                                                                            atcaaACGGTGGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTc                                                                           <  1:951084/150‑1 (MQ=255)
                                                                             tcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                          <  2:1880605/150‑1 (MQ=255)
                                                                               aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGc                                                                        <  1:268507/150‑1 (MQ=255)
                                                                               aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGCCAGCGGTATTCACACTCAGc                                                                        >  1:1833535/1‑150 (MQ=255)
                                                                               aaaCGGTTGAAAACGTGAAGCGGATTATTAAGGAGTGGATTCGCCGACGGTAAAGATGCCGGAGGAGGTTGGAACATCCTGCGGCTACATGCTGACACTATAGTCATTACTCTGCCGTTACAGACACCGTCTGCGGTAGTCACACTcccc                                                                        >  2:808375/1‑147 (MQ=255)
                                                                                       taaaaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                                <  1:638781/149‑1 (MQ=255)
                                                                                          taggTGCTGCGGATTATTATTGAGGGTATTCGACCACGTTATAGATGGAGGAGGAGGTTGTAAAATCCTCCGGCTACATGTTTAACCTATAGTCATTAAGAGGGCGTTACCGCAAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGat                                                             <  1:93175/147‑1 (MQ=255)
                                                                                          aaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGat                                                             <  1:667449/150‑1 (MQ=255)
                                                                                            cGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTTAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGatat                                                           <  1:481583/150‑1 (MQ=255)
                                                                                               gCAGCGGATTATTTTTGTGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACa                                                        <  2:1335623/150‑1 (MQ=255)
                                                                                               gCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACa                                                        >  2:276916/1‑150 (MQ=255)
                                                                                                     gATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGtt                                                  <  1:1372049/150‑1 (MQ=255)
                                                                                                       ttattattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTcc                                                <  2:1502165/150‑1 (MQ=255)
                                                                                                          ttattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGa                                             >  2:759493/1‑150 (MQ=255)
                                                                                                              tGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGa                                         <  2:903694/150‑1 (MQ=255)
                                                                                                              tGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGa                                         <  2:567961/150‑1 (MQ=255)
                                                                                                               gagtcgATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGat                                        <  2:556711/145‑1 (MQ=255)
                                                                                                               gAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTACCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGat                                        <  2:1470157/150‑1 (MQ=255)
                                                                                                                 ggggATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGatat                                      >  1:696064/1‑150 (MQ=255)
                                                                                                                    gATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGc                                   >  1:313710/1‑150 (MQ=255)
                                                                                                                        cGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                               >  1:1355385/1‑150 (MQ=255)
                                                                                                                        cGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                               >  1:712059/1‑150 (MQ=255)
                                                                                                                        cGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                               >  2:1104605/1‑150 (MQ=255)
                                                                                                                        cGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                               >  1:745511/1‑150 (MQ=255)
                                                                                                                         gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                              <  1:1581076/150‑1 (MQ=255)
                                                                                                                         gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                              <  1:755973/150‑1 (MQ=255)
                                                                                                                         gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                              >  2:633660/1‑150 (MQ=255)
                                                                                                                              cGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATACACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                         <  2:460659/150‑1 (MQ=255)
                                                                                                                                 tAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGc                      >  1:740587/1‑150 (MQ=255)
                                                                                                                                    ggtagCCGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCaaa                   >  1:1210228/5‑150 (MQ=255)
                                                                                                                                       tGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGTGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAcc                >  1:1698143/1‑150 (MQ=255)
                                                                                                                                        gCCGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAccc               >  2:960631/1‑150 (MQ=255)
                                                                                                                                        gCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAAGCTCGATATACCGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGcttcaccc               >  2:1244652/1‑143 (MQ=255)
                                                                                                                                         ccGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCCGCGGCAGCGGTAGTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCTCGGGCAGAGcccg              >  1:114389/1‑149 (MQ=255)
                                                                                                                                         ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGGCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt              <  2:1837826/150‑1 (MQ=255)
                                                                                                                                           ggaggaGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGc            >  1:727708/1‑150 (MQ=255)
                                                                                                                                             aggaggTTGAAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGAGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAc          >  1:1106701/1‑150 (MQ=255)
                                                                                                                                              ggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAcc         <  1:759493/150‑1 (MQ=255)
                                                                                                                                              ggaggTTGTAACAACCTCCGGCTACCTGTATAAACTATAGTCATTAAGCTCGCGTTACCGCCAGCGGCAGCGGTAGTCTCACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGAAATTGCTTTCGCCACGGGCACACCCCAGCAcc         >  2:77011/1‑150 (MQ=255)
                                                                                                                                                 gggtGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCTTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAAGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGaa      <  2:333210/147‑1 (MQ=255)
                                                                                                                                                 ggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGTTATTCAGCCGTTCCAGATCGATATTGCTGTCGCCACGGGCAAAACCCTGCAACGaa      >  2:172042/1‑150 (MQ=255)
                                                                                                                                                  gTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGaaa     <  1:660427/150‑1 (MQ=255)
                                                                                                                                                   ttGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAAc    >  2:57566/1‑150 (MQ=255)
                                                                                                                                                   ttGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAAc    >  1:848735/1‑150 (MQ=255)
                                                                                                                                                     gTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACCCTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCCCGGGCAAAACCCTGCGCCGAAACaa  >  2:109055/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAA  >  NC_000913/1074749‑1075047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: