Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,141,656 T→A 5.2% E904D (GAA→GAT rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,6560TA 5.2% 348.9 / 4.6 128E904D (GAA→GATrneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (65/54);  new base A (3/4);  total (69/59)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 130 total aligned reads displayed.

GTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTG  >  NC_000913/1141507‑1141805
                                                                                                                                                     |                                                                                                                                                     
gTCGCTTCCTGCGGTTCAACCTCCGGTTCTGCTTGTTCTTCCACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGt                                                                                                                                                       <  1:582505/150‑1 (MQ=255)
  ctctTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTGCATGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGTATGCGTCGTTTCAACCAATTCCGGTTGCGGTTc                                                                                                                                                     >  1:1447824/1‑150 (MQ=255)
    ctTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCgg                                                                                                                                                   <  1:1018791/150‑1 (MQ=255)
    ctTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCgg                                                                                                                                                   <  2:1646627/150‑1 (MQ=255)
    ctTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCgg                                                                                                                                                   <  2:702070/150‑1 (MQ=255)
    ctTCCTGCGGTTCAACAACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCCTATCAACCACTTCCGGTTGCGGTTCgg                                                                                                                                                   >  2:864803/1‑150 (MQ=255)
      tCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCa                                                                                                                                                 >  1:1308414/1‑150 (MQ=255)
         tGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAAcc                                                                                                                                              <  1:1729635/150‑1 (MQ=255)
          gCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCCGAACCGCAAccg                                                                                                                                             <  1:1035041/150‑2 (MQ=255)
            ggTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGa                                                                                                                                           >  1:1639060/1‑150 (MQ=255)
            ggTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGCTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCCGAACCGCAACCGGa                                                                                                                                           <  1:205819/150‑1 (MQ=255)
                cAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACgg                                                                                                                                       >  1:634289/1‑150 (MQ=255)
                 aaCCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACggg                                                                                                                                      >  1:942816/1‑150 (MQ=255)
                    caccGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGc                                                                                                                                   <  2:623089/150‑1 (MQ=255)
                      ccGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCtt                                                                                                                                 >  1:1097955/1‑150 (MQ=255)
                         gTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGAAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGATGCGGTTCGGCAACCTGAACGGGGGCTTCTa                                                                                                                              <  1:1212968/150‑1 (MQ=255)
                          ttCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTAc                                                                                                                             <  1:647177/150‑1 (MQ=255)
                           gcTGCGTGTGCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTCATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCATGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACg                                                                                                                            <  1:294385/149‑1 (MQ=255)
                            cTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTACTCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCCGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGa                                                                                                                           <  1:89013/150‑1 (MQ=255)
                            cTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGa                                                                                                                           >  2:1356216/1‑150 (MQ=255)
                             tGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTg                                                                                                                                            <  2:1334978/132‑1 (MQ=255)
                             tGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGac                                                                                                                          >  2:1568327/1‑150 (MQ=255)
                             tGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGac                                                                                                                          >  2:1418024/1‑150 (MQ=255)
                             tGCGTGTGCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGTGGCTTCTACGac                                                                                                                          <  1:376006/150‑1 (MQ=255)
                               cTTGTTCTGCAACTTCCTGTGCTACGGCAACTTCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGacac                                                                                                                        <  2:140393/150‑1 (MQ=255)
                                ttGTTCTGCAACTTCCTGGGCTACGGCAACATCTGACTCGGTTATCACCTGCGGCTGTTCAGTTACCGCGGCTGCGAGCTCTTCAGGATGCGTCGTTTCAACCTCTTCCGGTTGCGGTTCGGCACCCTGCCCGGGGGATTCTACGACAcc                                                                                                                       >  1:106249/1‑150 (MQ=255)
                                ttGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACAcc                                                                                                                       >  1:508090/1‑150 (MQ=255)
                                 tGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTTCCGCGGCAGCGATCACTTCAGGATGCGTCGATACAACCACTTCCGGTTGAGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCg                                                                                                                      >  1:63603/1‑150 (MQ=255)
                                 tGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCACCCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCg                                                                                                                      >  1:175087/1‑150 (MQ=255)
                                  gTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCgg                                                                                                                     <  2:241064/150‑1 (MQ=255)
                                    tCTGCTACTTCCTGGGCTACGGCAACATCAGCCTCGGTAATCACCAGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGcc                                                                                                                   <  2:344904/150‑1 (MQ=255)
                                    tCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGcc                                                                                                                   <  2:1776765/150‑1 (MQ=255)
                                    tCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGcc                                                                                                                   <  2:329759/150‑1 (MQ=255)
                                     cagCAACTTGCTGGGCTACGGCAACATCAGACTCCGTAAACACCTGCGGCTGTTCAGTTACAGCGGAAACGATCACTGCAGGATGCGTCGTTTCATCCACTTCCGGTTTCTGTTCGGCAAACTGAACGGGTGCTTCTACGACAACGGcgt                                                                                                                  >  2:838957/3‑148 (MQ=255)
                                     cTGCAACTTCCTGGGCTACGGCAACTTCAGACTCGGTAATGACCTGCGGCTGTTCAGTTACCGCGGCAGCTATCACTTCTGGATGCGTCGTTTCAACCACTTCCGGTTTCGGTGCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCa                                                                                                                  <  2:1108680/150‑1 (MQ=255)
                                     cTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCATCCACTTCCGGTTGCGGTTCGGCACCCTGAACGGGGGCTTCTACGACACCGGcgc                                                                                                                  >  2:831654/1‑148 (MQ=255)
                                     cTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCa                                                                                                                  >  2:1317926/1‑150 (MQ=255)
                                      tGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCCACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCAc                                                                                                                 >  2:1355746/1‑150 (MQ=255)
                                       gCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCAct                                                                                                                <  1:851801/150‑1 (MQ=255)
                                       gCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCAct                                                                                                                >  2:1353999/1‑150 (MQ=255)
                                       gCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACGTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGCGCTTCTACGACACCGGCCAct                                                                                                                >  2:1574001/1‑150 (MQ=255)
                                         aaCTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCActtc                                                                                                              <  2:1355258/150‑1 (MQ=255)
                                         aaCTACCTGGGCTACGGCAACATCAGACTCGCGACTCACCTGCGGCGGGTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCActtc                                                                                                              <  1:85973/150‑1 (MQ=255)
                                            ttCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACttcttc                                                                                                           >  2:1060738/1‑150 (MQ=255)
                                             tCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTca                                                                                                          >  2:1721430/1‑150 (MQ=255)
                                               cTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTcaac                                                                                                        >  2:199923/1‑150 (MQ=255)
                                                      aCGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGgc                                                                                                 >  2:1379456/1‑150 (MQ=255)
                                                       cGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGgcg                                                                                                >  1:1192913/1‑150 (MQ=255)
                                                          cAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGCCACCGGCCACTTCTTCAACAACTGgcgcgc                                                                                             >  2:156874/1‑150 (MQ=255)
                                                          cAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTAACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAAAGGGGGCTTCAACGAAACCGCCCACTTCTTAAACAACTTgcgcgc                                                                                             >  1:191762/1‑150 (MQ=255)
                                                              aTCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTaac                                                                                         >  2:1092226/1‑150 (MQ=255)
                                                               tCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTaaca                                                                                        >  2:1095266/1‑150 (MQ=255)
                                                               tCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTaaca                                                                                        >  2:660898/1‑150 (MQ=255)
                                                                  gACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAccc                                                                                     <  2:1869388/150‑1 (MQ=255)
                                                                  gACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAAcc                                                                                     <  1:327365/150‑1 (MQ=255)
                                                                  gACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAAcc                                                                                     >  2:1448354/1‑150 (MQ=255)
                                                                     tCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGt                                                                                  <  1:1677524/150‑1 (MQ=255)
                                                                      cGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCGACGCCACCGGGCCCGTCTTCAACTACTTGCGCGCTAACAACCTGtt                                                                                 >  1:952626/1‑150 (MQ=255)
                                                                        gTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCg                                                                               <  1:719681/150‑1 (MQ=255)
                                                                        gTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCg                                                                               <  2:1858442/150‑1 (MQ=255)
                                                                           aTCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATa                                                                            >  1:16595/1‑150 (MQ=255)
                                                                             cACCTGCGGCTGTTCAGTTACCGTGGCAGCGATGACTTCAGGCGGGGTCTTTTCAACAACTTCCGGTTGCGGGTCGGCAACCTGAACGGGGGCTTCTAAGACATAGGACACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAgc                                                                          <  2:1227606/150‑1 (MQ=255)
                                                                               ccTGCGGCTGTTCAGTTACCGCGGCAGAGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTGGATTgcgg                                                                        >  1:918971/1‑150 (MQ=255)
                                                                                 tgaggCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAgcggcg                                                                      <  2:553077/147‑1 (MQ=255)
                                                                                 tGCGGCTGTTCAGTTACCGCGGCAGAGATCACTTCAGGATGCGTCGTTTCCACCACTTCCGGTTGCGGTTCGGCCACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTACCAACCGGGTCGATAgcggcg                                                                      >  1:1352869/1‑150 (MQ=255)
                                                                                         tttaGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAgg                                                              >  1:678507/4‑150 (MQ=255)
                                                                                           aaGTTACCGCGGCAGCGATCACTTCAGGATGCGGCGTTTCTACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGa                                                            <  2:891192/149‑1 (MQ=255)
                                                                                                aCCGCGGCAGCGATAACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGTATCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCa                                                       <  2:58735/150‑1 (MQ=255)
                                                                                                 ccGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAg                                                      <  2:1909156/150‑1 (MQ=255)
                                                                                                 ccGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAg                                                      >  1:189714/1‑150 (MQ=255)
                                                                                                 ccGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAg                                                      >  2:1592525/1‑150 (MQ=255)
                                                                                                 ccGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCCGCCACTTATGCAACACCTCGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGCACCTCAg                                                      >  1:1327691/1‑150 (MQ=255)
                                                                                                  cgcgGCAGCGATCACATCAGGATGCGTCGTCGCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGCGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGt                                                     <  1:236668/150‑1 (MQ=255)
                                                                                                     ggCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCAc                                                  <  1:1317926/150‑1 (MQ=255)
                                                                                                      gCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCAcc                                                 >  1:1260296/1‑150 (MQ=255)
                                                                                                          cGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCg                                             >  2:859422/1‑150 (MQ=255)
                                                                                                            aTCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTTACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGc                                           <  1:717376/150‑1 (MQ=255)
                                                                                                                cTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACCCCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCAGCGGCTGcc                                       >  2:1252784/1‑149 (MQ=255)
                                                                                                                cTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGca                                       >  1:521624/1‑150 (MQ=255)
                                                                                                                cTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGca                                       >  1:569263/1‑150 (MQ=255)
                                                                                                                  tCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCCCCATCGGCTGcaca                                     >  1:1462127/1‑150 (MQ=255)
                                                                                                                        tGCGTCGTTTCACACACTTCCGGTTGCGGTTCGGCAACATGAAAGGGGGATTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACAGTTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATAGTCTGCACATGTAct                               >  1:994924/1‑150 (MQ=255)
                                                                                                                         gCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTActt                              >  2:1365430/1‑150 (MQ=255)
                                                                                                                           gtcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCTTCGGCTGCACATGTtcttct                            >  1:737612/1‑150 (MQ=255)
                                                                                                                             cgtTTCAACCACTTCCGGTTGCGGTTCGGCACCCTGAACGGGGGCTTCTCCGACCCCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACGATCGGCTGCACATGTACgtcttc                          >  2:1334797/1‑150 (MQ=255)
                                                                                                                              gtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTcc                         <  2:1097955/150‑1 (MQ=255)
                                                                                                                              gtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTcc                         <  1:1356216/150‑1 (MQ=255)
                                                                                                                              gtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTcc                         >  2:529803/1‑150 (MQ=255)
                                                                                                                                ttCAACCACTTCCGGTTGCGGTTCTGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCAAACAACCTGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTg                       <  1:1686283/150‑1 (MQ=255)
                                                                                                                                 tccaCCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGt                      <  2:1228662/147‑1 (MQ=255)
                                                                                                                                      cACTTCCGGTTGCGGTTCGGCAACCGGAACGTGGGCTTCTTCGACACCGGCCACTTCTACAACACCTGGCGCGCGAACAACCGGGGCGATTGCGGGGGCGGCAGGGCCCTGTGTCGGATTGGGCTGGAGATGTACTTCTTGCTGGTcggg                 >  2:1381309/1‑148 (MQ=255)
                                                                                                                                       cgttCAGGTTGCCGAACCGCAACCGGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCgcgc                >  1:26666/3‑150 (MQ=255)
                                                                                                                                        gttCAGGTTGCCGAACCGCAACCGGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACACCTGGCGCGCTAACACCCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACGTCTTCCTGTTCGCgct               >  1:728917/2‑150 (MQ=255)
                                                                                                                                           ccGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCgctgct            <  2:687814/150‑1 (MQ=255)
                                                                                                                                             ggTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTACCAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGctct          <  1:614380/150‑1 (MQ=255)
                                                                                                                                               ttGCGGTGCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTc        <  2:223746/150‑1 (MQ=255)
                                                                                                                                                tGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCATCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGGCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCa       <  2:788946/150‑1 (MQ=255)
                                                                                                                                                   ggTTCGGCAACCGGAACGGGGGCATCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAAcc    <  2:332593/150‑1 (MQ=255)
                                                                                                                                                    gTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCt   >  2:1864504/1‑150 (MQ=255)
                                                                                                                                                     ttCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTg  <  1:3439/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTG  >  NC_000913/1141507‑1141805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: