Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,192,685 A→C 5.1% R363R (CGT→CGG mnmA ← tRNA(Gln,Lys,Glu) U34 2‑thiouridylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,192,6850AC 5.1% 300.2 / 3.8 105R363R (CGT→CGGmnmAtRNA(Gln,Lys,Glu) U34 2‑thiouridylase
Reads supporting (aligned to +/- strand):  ref base A (56/43);  new base C (3/3);  total (59/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 105 total aligned reads displayed.

TCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCT  >  NC_000913/1192537‑1192833
                                                                                                                                                    |                                                                                                                                                    
tCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGAc                                                                                                                                                     <  2:128769/150‑1 (MQ=255)
     aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACgctgc                                                                                                                                                <  2:1843657/150‑1 (MQ=255)
     aTGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACgctgc                                                                                                                                                >  1:1680682/1‑150 (MQ=255)
      tGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGTCTGCCgctgcc                                                                                                                                               <  1:782940/150‑2 (MQ=255)
      tGCCCCTGGTGAGCGAGTTGATGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGTCTGCCgctgcc                                                                                                                                               <  1:1303517/150‑2 (MQ=255)
       gCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTc                                                                                                                                              <  1:955454/150‑1 (MQ=255)
        ccccTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCa                                                                                                                                             <  1:489889/150‑1 (MQ=255)
          ccTGGTGAGCGCGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGTCTGCCgctgccggtc                                                                                                                                           <  2:582083/150‑6 (MQ=255)
           cTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCaata                                                                                                                                          <  1:328765/150‑1 (MQ=255)
             ggTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCaataat                                                                                                                                        <  1:894703/150‑1 (MQ=255)
                  gCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGc                                                                                                                                   <  2:1439980/150‑1 (MQ=255)
                    taGTTGTTGCACCAGGCGTGCCGACTGAAAAATACAGGCAAGGGCGAGGGTTATTTCATAGTAATTCTTTGCCACGTTAACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCa                                                                                                                                 <  2:375359/149‑1 (MQ=255)
                      gttgttGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTCTAAGTAAAGATACTAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCAcc                                                                                                                               <  2:294224/150‑1 (MQ=255)
                         gttgCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAg                                                                                                                            >  2:578637/1‑150 (MQ=255)
                           tgCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCAGCAGACGCTGCTCAATAATACCGCCACCGAGGc                                                                                                                          <  2:592395/150‑1 (MQ=255)
                            gCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGca                                                                                                                         <  1:666754/150‑1 (MQ=255)
                             cACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGcac                                                                                                                        >  1:1405187/1‑150 (MQ=255)
                                  ggCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTc                                                                                                                   <  2:443203/150‑1 (MQ=255)
                                       gCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGGCATAGTAATTCTTTGCCACGTTAACTGCTTCCTTGTTTTAAGTATAGATAATAATCAGACCGGCAGCGGCAGCCGCTGCTCAATAATGCCGACACCGAGGCACACTTCACCGt                                                                                                              >  2:114049/1‑150 (MQ=255)
                                        ccGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGtt                                                                                                             <  1:535581/150‑1 (MQ=255)
                                         cGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTta                                                                                                            >  2:1567499/1‑150 (MQ=255)
                                          gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATAGCGCCACCGAGGCACACTTCACCGTtat                                                                                                           <  1:42850/150‑1 (MQ=255)
                                              gACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGCCGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATagaa                                                                                                       >  2:1780448/1‑150 (MQ=255)
                                              gACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATagaa                                                                                                       >  2:11497/1‑150 (MQ=255)
                                              gACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACAGTTATagaa                                                                                                       >  2:1170877/1‑150 (MQ=255)
                                                cAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATagaagc                                                                                                     >  1:1619596/1‑149 (MQ=255)
                                                  aaTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACTCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACg                                                                                                   <  2:1173213/150‑1 (MQ=255)
                                                  aaTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCATCGAGGCACACTTCACCGTTATAGAAGACg                                                                                                   <  2:1787846/150‑1 (MQ=255)
                                                   aTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACgg                                                                                                  >  1:1544371/1‑150 (MQ=255)
                                                   aTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGATCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACgg                                                                                                  >  2:177278/1‑150 (MQ=255)
                                                    tACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGc                                                                                                 <  1:418332/150‑1 (MQ=255)
                                                     aCCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGATTCCTTGTTTTAAGTAAAGATAATAATCAGACAGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGATATAGAAGACGGCa                                                                                                >  2:1892872/1‑150 (MQ=255)
                                                     aCCGGCCAGGGCGAGGGTGATGTCATAGAAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACAGAGGCACACTTCACCGTTATAGAAGACGGCa                                                                                                >  2:369053/1‑150 (MQ=255)
                                                      ccGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAg                                                                                               >  1:1574537/1‑150 (MQ=255)
                                                      ccGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAg                                                                                               >  2:179707/1‑150 (MQ=255)
                                                        ggCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGAc                                                                                             <  2:1675412/150‑1 (MQ=255)
                                                         gCCAGGGCGAGGGAGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCATACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACt                                                                                            <  2:1098403/150‑1 (MQ=255)
                                                          ccAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGCCCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTg                                                                                           >  2:383383/1‑150 (MQ=255)
                                                          ccAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTg                                                                                           >  2:606702/1‑150 (MQ=255)
                                                            aGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGc                                                                                         >  1:1076747/1‑150 (MQ=255)
                                                                  aGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCg                                                                                   <  2:1580718/150‑1 (MQ=255)
                                                                  aGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCg                                                                                   <  1:618604/150‑1 (MQ=255)
                                                                  aGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCg                                                                                   <  1:1582141/150‑1 (MQ=255)
                                                                   gggTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGt                                                                                  >  1:783140/1‑150 (MQ=255)
                                                                          gTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcggcg                                                                           >  2:1790946/1‑150 (MQ=255)
                                                                          gTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcggcg                                                                           <  1:1071005/150‑1 (MQ=255)
                                                                           tCATAGTAATTCTTTGCCACGTTCACTGCTTCATTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGCCGGCAGCCTGGCCCGGCGTCAcggcgg                                                                          >  2:1144772/1‑150 (MQ=255)
                                                                            cATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACggcggc                                                                         <  2:1405187/150‑1 (MQ=255)
                                                                            cATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACggcggc                                                                         >  2:909649/1‑150 (MQ=255)
                                                                            cATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACggcggc                                                                         >  2:127393/1‑150 (MQ=255)
                                                                                gTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCCCCGAGGCACACTTCACCGTTATAGAAGACGGCAGCCTGGCCCGGCGTCCCGGCGGCAAcc                                                                     >  2:1088237/1‑150 (MQ=255)
                                                                                gTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCCCGGCGGCAAcc                                                                     >  2:1278311/1‑150 (MQ=255)
                                                                                  aaTTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCgg                                                                   >  2:1587015/1‑150 (MQ=255)
                                                                                    ttCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGtt                                                                 <  2:598545/150‑1 (MQ=255)
                                                                                    ttCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGtt                                                                 <  2:1544371/150‑1 (MQ=255)
                                                                                     tCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTc                                                                >  2:180991/1‑150 (MQ=255)
                                                                                     tCTTTGCCACGGTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTc                                                                <  1:780519/150‑1 (MQ=255)
                                                                                        ttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc                                                             <  1:1084876/150‑1 (MQ=255)
                                                                                         tggcACGTTCACTGCTTCCTTTTTTTAATTAAAGATAATAATCAGACCGGCATCGGCAGACGGTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATATAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCg                                                            <  2:1605711/147‑1 (MQ=255)
                                                                                         tGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCg                                                            >  2:739222/1‑150 (MQ=255)
                                                                                           ccACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTAATAGAAGACGGCAGACTTGCCCGGCGTCACGGCGGCAACCGGTTCATCGaa                                                          >  2:1838488/1‑150 (MQ=255)
                                                                                           ccACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTACACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCCGCAACCGGTTCATCGaa                                                          >  1:62637/1‑150 (MQ=255)
                                                                                                  cACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCTAAAATCACt                                                   >  1:1551998/1‑150 (MQ=255)
                                                                                                    cTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGTCACACTTCACCGTTATAGAATCCGGGCGACTGGCCCGTCGTCACGGCGGCATCCGGTTGATCGAAAATcccgta                                                 >  1:93008/1‑145 (MQ=255)
                                                                                                     tGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACAATTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCACCCGGTTCATCGAAAATCACTTCa                                                >  1:1616599/1‑150 (MQ=255)
                                                                                                       cTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCACCCGGTTCATCGAAACTCACTTCAAt                                              >  2:1403104/1‑150 (MQ=255)
                                                                                                       cTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAAt                                              >  2:1612905/1‑150 (MQ=255)
                                                                                                       cTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGGTCATCGAAAATCACTTCAAt                                              >  2:474088/1‑150 (MQ=255)
                                                                                                       cTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCAACGTTATAGAGGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCaaa                                              >  1:1659783/1‑149 (MQ=255)
                                                                                                             tGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatc                                        <  1:1612905/150‑1 (MQ=255)
                                                                                                             tGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatc                                        >  1:837742/1‑150 (MQ=255)
                                                                                                               ttttAAGTAAAGATAATAAACCGACCGGCAGCGGCAGACGCTGCTCTATAATACCGCCACCGCGGCACACTTCACCGTTATAGAAGAAGGCAGACTGGCCCGGCGTCACCGCGGCAACAGGTTCATCGAAAATAACATCAATGCTatcat                                      >  2:126942/1‑150 (MQ=255)
                                                                                                                tttAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcatc                                     >  1:1873547/1‑150 (MQ=255)
                                                                                                                   aaGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTc                                  <  1:184301/150‑1 (MQ=255)
                                                                                                                    aGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTcc                                 <  2:1765157/150‑1 (MQ=255)
                                                                                                                       aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAGCCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAgc                              >  1:455514/1‑150 (MQ=255)
                                                                                                                         aGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGAGCATCGTCCAgcgc                            >  2:735347/1‑150 (MQ=255)
                                                                                                                          gATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGCCTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGcc                           >  2:1364751/1‑150 (MQ=255)
                                                                                                                           ataataATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCt                          <  2:1729825/150‑1 (MQ=255)
                                                                                                                            taataaTCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCtt                         <  1:1567499/150‑1 (MQ=255)
                                                                                                                             aataatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTg                        <  1:1806833/150‑1 (MQ=255)
                                                                                                                             aataatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCGTg                        >  2:757521/1‑150 (MQ=255)
                                                                                                                                aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACg                     >  1:433804/1‑150 (MQ=255)
                                                                                                                                aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATAGTCCAGCGCCTTGaag                     >  2:1047612/1‑148 (MQ=255)
                                                                                                                                aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGGTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACg                     >  1:1346299/1‑150 (MQ=255)
                                                                                                                                aatCAGACCGGCAGCGACAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGACAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACg                     <  2:1230655/150‑1 (MQ=255)
                                                                                                                                   cAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTg                  >  1:955770/1‑150 (MQ=255)
                                                                                                                                   cAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTg                  <  2:1728745/150‑1 (MQ=255)
                                                                                                                                    tattgagcagcgtctgCCGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGc                 >  2:1204142/7‑150 (MQ=255)
                                                                                                                                    cgACCGGCCGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGc                 <  2:838609/149‑1 (MQ=255)
                                                                                                                                    aGACCGGCCGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGc                 <  2:173488/150‑1 (MQ=255)
                                                                                                                                        cggcagcggcagACGCTGCTCAATAATACCGCCACCGAGTCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAgg             <  2:1680682/150‑1 (MQ=255)
                                                                                                                                        cggcagcggcagACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAgg             <  1:959246/150‑1 (MQ=255)
                                                                                                                                        cggcagcggcagACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAgg             <  2:862167/150‑1 (MQ=255)
                                                                                                                                             gcggcagACGCTGCTCAATAATACCGCCCCCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCCCTTCAATGCGCTCATCGGCCAGCGCCTTGACGGGGCAAGGGatat        >  2:684709/1‑150 (MQ=255)
                                                                                                                                             gcggcagACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGatat        >  1:598164/1‑150 (MQ=255)
                                                                                                                                              cggcagACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATc       >  2:1362594/1‑150 (MQ=255)
                                                                                                                                               ggcagACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCg      >  2:1151729/1‑150 (MQ=255)
                                                                                                                                                 cagACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGt    >  1:1673645/1‑150 (MQ=255)
                                                                                                                                                   gACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCt  >  2:59867/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCT  >  NC_000913/1192537‑1192833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: